ec 50 calculation prism

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PREPARING AND INTEPRETING CYTOTOXICITY DATA 11175-A FLINTKOTE AVENUE, SAN DIEGO, CA 92121 TELEPHONE (858) 642-1988 • FAX (858) 642-1989 WWW.ATSBIO.COM • [email protected] ADVANCED TARGETING SYSTEMS After 30 minutes in the incubator, read your plates on a plate reader using SoftMax Pro (or equivalent) software. If the mean A490 of the control wells on plate is >0.250, the assay is complete. If the mean A490 of the control wells on plate is <0.250, return the plates to the incubator for another 30 minutes, then read again. Continue reading the plates every 25-35 minutes until the mean A490 of the control wells on plate is >0.250. This is a standard readout using SOFTmax Pro. (You should notice a color change in the wells when the assay is complete. See “Cytotoxicity Assay for Targeted Toxins In Vitro”) Highlight the “% Control” and “CV%” columns and “Copy.” Use the software to calculate “Result,” “% Control,” Standard deviation “stddev,” and Coefficient of Variation “CV%.” 4/24/2008 Page 1 of 4

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Page 1: EC 50 Calculation Prism

7/27/2019 EC 50 Calculation Prism

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