draft risk evaluation for perchloroethylene (ethene, 1,1,2,2 … · 2020-04-27 · united states...
TRANSCRIPT
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United States Office of Chemical Safety and Environmental Protection Agency Pollution Prevention
Draft Risk Evaluation for Perchloroethylene
(Ethene, 1,1,2,2-Tetrachloro)
CASRN: 127-18-4
Systematic Review Supplemental File: Data Quality Evaluation of Ecological Hazard Studies
NOTICE: This information is distributed solely for the purpose of pre-dissemination peer review under applicable information quality guidelines. It has not been formally disseminated by EPA. It does not represent and should not be construed to represent any Agency determination or policy. It is being circulated for review of its technical accuracy and science policy implications.
April 2020, DRAFT
PEER REVIEW DRAFT. DO NOT CITE OR QUOTE
-
Tableof
Contents
HERO
IDD
ata
Typ
eR
efer
ence
1
7508
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Leb
lan
c,G
.A
..1980.
Acu
teto
xic
ity
of
pri
ori
typ
ollu
tants
tow
ate
rfl
ea(D
ap
h-
nia
magn
a).
Bu
llet
inof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
24:6
84-
691
1
12017
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Bro
der
ius,
S.,K
ah
l,M
..1985.
Acu
teto
xic
ity
of
org
an
icch
emic
al
mix
ture
sto
the
fath
ead
min
now
.A
qu
ati
cT
oxic
olo
gy
6:3
07-3
22
4
12017
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
Bro
der
ius,
S.,
Kah
l,M
..1985.
Acu
teto
xic
ity
of
org
an
icch
emic
al
mix
ture
sto
the
fath
ead
min
now
.A
qu
ati
cT
oxic
olo
gy
6:3
07-3
22
6
18050
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
Barr
ow
s,M
.E
.,P
etro
celli,
S.
R.,M
ace
k,
K.
J.,C
arr
oll,
J.
J..
1980.
Bio
con
cen
-tr
ati
on
an
del
imin
ati
on
of
sele
cted
wate
rp
ollu
tants
by
blu
egillsu
nfi
sh(L
epom
ism
acr
och
iru
s).
8
18064
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Bu
ccafu
sco,
R.
J.,E
lls,
S.
J.,L
ebla
nc,
G.
A..
1981.
Acu
teto
xic
ity
of
pri
ori
typ
ollu
tants
tob
lueg
ill
(Lep
om
ism
acr
och
iru
s).
Bu
llet
inof
Envir
on
men
tal
Con
-ta
min
ati
on
an
dT
oxic
olo
gy
26:4
46-4
52
11
18110
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Hei
tmu
ller
,P
.T
.,H
ollis
ter,
T.
A.,P
arr
ish
,P
.R
..1981.
Acu
teto
xic
ity
of
54
ind
ust
rial
chem
icals
tosh
eep
shea
dm
inn
ow
s(C
yp
rin
od
on
vari
egatu
s).
Bu
llet
inof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
27:5
96-6
04
14
32169
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Gei
ger
,D
.L
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ort
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tt,
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E.,C
all,
D.
J.,B
rooke,
L.
T.
eds.
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Acu
teto
xi-
citi
esof
org
an
icch
emic
als
tofa
thea
dm
inn
ow
s(P
imep
hale
sp
rom
elas)
:volu
me
II.
16
42313
Oth
er;
Ter
rest
rial;
Die
tz,
A.
C.,S
chn
oor,
J.
L..
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Phyto
toxic
ity
of
chlo
rin
ate
dalip
hati
csto
hyb
rid
pop
lar
(Pop
ulu
sd
elto
ides
xn
igra
DN
34).
Envir
on
men
tal
Toxic
olo
gy
an
dC
hem
istr
y20:3
89-3
93
19
48608
Acu
te(0
-96
hou
r);
Ter
rest
rial;
San
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,S
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yev
alu
ati
on
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chem
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com
mon
lyfo
un
dat
haza
rdou
sin
du
stri
al
wast
esi
tes.
DN
AR
epair
224:4
37-4
45
21
58126
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Ale
xan
der
,H
.C
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cCart
y,W
.M
.,B
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,E
.A
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Toxic
ity
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-ch
loro
ethyle
ne,
tric
hlo
roet
hyle
ne,
1,1
,1-t
rich
loro
eth
an
e,an
dm
ethyle
ne
chlo
rid
eto
fath
ead
min
now
s.B
ullet
inof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
20:3
44-3
52
23
64580
Oth
er;
Ter
rest
rial;
Ber
nard
,A
.M
.,d
eR
uss
is,R
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orm
an
d,J.C
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auw
erys,
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ati
on
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the
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acu
ten
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roto
xic
ity
of
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oh
exan
ean
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erin
du
stri
al
solv
ents
inth
efe
male
Sp
ragu
e-D
aw
ley
rat.
Toxic
olo
gy
Let
ters
45:2
71-2
80
25
i
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
76052
Ch
ron
ic(>
21
days)
;T
erre
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enin
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Dev
elop
men
tal
toxic
ity.
Jou
rnal
of
Toxic
olo
gy
an
dE
nvi-
ron
men
tal
Hea
lth
45:1
45-1
71
27
94468
Oth
er;
Ter
rest
rial;
Elo
vaara
,E
.,H
emm
inki,
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ain
io,
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Eff
ects
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met
hyle
ne
chlo
rid
e,tr
ich
loro
eth
an
e,tr
ich
loro
ethyle
ne,
tetr
ach
loro
ethyle
ne
an
dto
luen
eon
the
de-
vel
op
men
tof
chic
kem
bry
os.
Toxic
olo
gy
12:1
11-1
19
29
95201
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Sm
ith
,A
.D
.,B
hara
th,
A.,M
allard
,C
.,O
rr,
D.,S
mit
h,
K.,S
utt
on
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an
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cCart
y,L
.S
.,O
zbu
rn,
G.
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Th
eacu
tean
dch
ron
icto
xic
ity
of
ten
chlo
rin
ate
dorg
an
icco
mp
ou
nd
sto
the
Am
eric
an
flagfi
sh(J
or-
dan
ella
flori
dae)
.A
rch
ives
of
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
20:9
4-1
02
31
200570
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
San
chez
-Fort
un
,S
.,S
an
z,F
.,S
anta
-Mari
a,
A.,R
os,
J.
M.,D
eV
icen
te,
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nas,
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,E
.,B
ara
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a,
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Acu
tese
nsi
tivit
yof
thre
eage
class
esof
Art
emia
salin
ala
rvae
tose
ven
chlo
rin
ate
dso
lven
ts.
Bu
l-le
tin
of
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
59:4
45-4
51
33
629907
Acu
te(0
-96
hou
r);
Ter
rest
rial;
Vale
nci
a,
R.,M
aso
n,
J.
M.,W
ood
ruff
,R
.C
.,Z
imm
erin
g,
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Ch
emic
al
mu
tagen
esis
test
ing
inD
roso
ph
ila.
III.
Res
ult
sof
48
cod
edco
mp
ou
nd
ste
sted
for
the
Nati
on
al
Toxic
olo
gy
Pro
gra
m.
Envir
on
men
tal
Mu
tagen
esis
7:3
25-3
48
36
632863
Oth
er;
Aqu
ati
c;F
ish
Sp
ence
r,H
.B
.,H
uss
ein
,W
.R
.,T
chou
nw
ou
,P
.B
..2002.
Eff
ects
of
tetr
a-
chlo
roet
hyle
ne
on
the
via
bilit
yan
dd
evel
op
men
tof
emb
ryos
of
the
Jap
an
ese
med
aka,
Ory
zias
lati
pes
.A
rch
ives
of
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxi-
colo
gy
42:4
63-4
69
38
632863
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Sp
ence
r,H
.B
.,H
uss
ein
,W
.R
.,T
chou
nw
ou
,P
.B
..2002.
Eff
ects
of
tetr
a-
chlo
roet
hyle
ne
on
the
via
bilit
yan
dd
evel
op
men
tof
emb
ryos
of
the
Jap
an
ese
med
aka,
Ory
zias
lati
pes
.A
rch
ives
of
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxi-
colo
gy
42:4
63-4
69
40
632864
Oth
er;
Aqu
ati
c;F
ish
Sp
ence
r,H
.B
.,H
uss
ein
,W
.R
.,T
chou
nw
ou
,P
.B
..2006.
Gro
wth
inh
ibit
ion
inJap
an
ese
med
aka
(Ory
zias
lati
pes
)fi
shex
pose
dto
tetr
ach
loro
ethyle
ne.
Jou
rnal
of
Envir
on
men
tal
Bio
logy
27
42
632864
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Sp
ence
r,H
.B
.,H
uss
ein
,W
.R
.,T
chou
nw
ou
,P
.B
..2006.
Gro
wth
inh
ibit
ion
inJap
an
ese
med
aka
(Ory
zias
lati
pes
)fi
shex
pose
dto
tetr
ach
loro
ethyle
ne.
Jou
rnal
of
Envir
on
men
tal
Bio
logy
27
45
657898
Acu
te(0
-96
hou
r);
Ter
rest
rial;
Cre
bel
li,
R.,
An
dre
oli,
C.,C
are
re,
A.,C
onti
,L
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roch
i,B
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a-R
am
usi
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enig
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Toxic
olo
gy
ofh
alo
gen
ate
dalip
hati
chyd
roca
rbon
s:S
tru
c-tu
ral
an
dm
ole
cula
rd
eter
min
ants
for
the
dis
turb
an
ceof
chro
moso
me
segre
ga-
tion
an
dth
ein
du
ctio
nof
lip
idp
eroxid
ati
on
.C
hem
ico-B
iolo
gic
al
Inte
ract
ion
s98:1
13-1
29
47
ii
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
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660091
Oth
er;
Ter
rest
rial;
Hu
lzeb
os,
E.
M.,A
dem
a,
D.
M.,D
irven
-Van
Bre
emen
,E
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enze
n,
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Van
Dis
,W
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erb
old
,H
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Phyto
toxic
ity
stu
die
sw
ith
Lact
uca
sati
va
inso
ilan
dnu
trie
nt
solu
tion
.E
nvir
on
men
tal
Toxic
olo
gy
an
dC
hem
istr
y12:1
079-1
094
49
660790
Acu
te(0
-96
hou
r);
Aqu
ati
c;P
lants
Bra
ck,
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ran
k,
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Ch
loro
phyll
afl
uore
scen
ce:
Ato
ol
for
the
inves
-ti
gati
on
of
toxic
effec
tsin
the
ph
oto
synth
etic
ap
para
tus.
Eco
toxic
olo
gy
an
dE
nvir
on
men
tal
Safe
ty40:3
4-4
1
51
661061
Acu
te(0
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hou
r);
Aqu
ati
c;P
lants
Bra
ck,
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ott
ler,
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Toxic
ity
test
ing
of
hig
hly
vola
tile
chem
icals
wit
hgre
enalg
ae:
An
ewass
ay.
1:2
23-2
28
54
661834
Acu
te(0
-96
hou
r);
Ter
rest
rial;
Miy
agaw
a,
M.,T
akasa
wa,
H.,S
ugiy
am
a,
A.,In
ou
e,Y
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ura
ta,
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no,
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osh
ikaw
a,
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1995.
Th
ein
viv
o-i
nvit
rore
plica
tive
DN
Asy
nth
esis
(RD
S)
test
wit
hh
epato
cyte
sp
rep
are
dfr
om
male
B6C
3F
1m
ice
as
an
earl
yp
red
ic-
tion
ass
ay
for
pu
tati
ve
non
gen
oto
xic
(Am
es-n
egati
ve)
mou
seh
epato
carc
inogen
s.M
uta
tion
Res
earc
h:
Gen
etic
Toxic
olo
gy
343:1
57-1
83
57
676758
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Yosh
ioka,
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Ose
,Y
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ato
,T
..1985.
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tin
gfo
rth
eto
xic
ity
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icals
wit
hT
etra
hym
ena
pyri
form
is.
Sci
ence
of
the
Tota
lE
nvir
on
men
t43:1
49-1
57
59
700434
Oth
er;
Aqu
ati
c;oth
eram
ph
ibia
n-
wood
frog
an
dgre
enfr
og
McD
an
iel,
T.,M
art
in,
P.,R
oss
,N
.,B
row
n,
S.,L
esage,
S.,P
auli
,B
..2004.
Eff
ects
of
chlo
rin
ate
dso
lven
tson
fou
rsp
ecie
sof
Nort
hA
mer
ican
am
ph
ibia
ns.
Arc
hiv
esof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
47:1
01-1
09
62
700434
Oth
er;
Aqu
ati
c;oth
eram
ph
ibia
n-
am
eric
an
toad
McD
an
iel,
T.,M
art
in,
P.,R
oss
,N
.,B
row
n,
S.,L
esage,
S.,P
auli
,B
..2004.
Eff
ects
of
chlo
rin
ate
dso
lven
tson
fou
rsp
ecie
sof
Nort
hA
mer
ican
am
ph
ibia
ns.
Arc
hiv
esof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
47:1
01-1
09
67
700434
Oth
er;
Aqu
ati
c;oth
eram
ph
ibia
n-
spott
edsa
lam
der
McD
an
iel,
T.,M
art
in,
P.,R
oss
,N
.,B
row
n,
S.,L
esage,
S.,P
auli
,B
..2004.
Eff
ects
of
chlo
rin
ate
dso
lven
tson
fou
rsp
ecie
sof
Nort
hA
mer
ican
am
ph
ibia
ns.
Arc
hiv
esof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
47:1
01-1
09
72
700434
Acu
te(0
-96
hou
r);
Aqu
ati
c;oth
erA
mp
hib
ian
McD
an
iel,
T.,M
art
in,
P.,R
oss
,N
.,B
row
n,
S.,L
esage,
S.,P
auli
,B
..2004.
Eff
ects
of
chlo
rin
ate
dso
lven
tson
fou
rsp
ecie
sof
Nort
hA
mer
ican
am
ph
ibia
ns.
Arc
hiv
esof
Envir
on
men
tal
Conta
min
ati
on
an
dT
oxic
olo
gy
47:1
01-1
09
78
707209
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Nie
der
leh
ner
,B
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air
ns,
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mit
h,
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Mod
elin
gacu
tean
dch
ron
icto
xi-
city
of
non
pola
rn
arc
oti
cch
emic
als
an
dm
ixtu
res
toC
erio
dap
hn
iad
ub
ia.
Eco
-to
xic
olo
gy
an
dE
nvir
on
men
tal
Safe
ty39:1
36-1
46
80
707209
Oth
er;
Aqu
ati
c;In
ver
teb
rate
sN
ied
erle
hn
er,
B.,C
air
ns,
J.,S
mit
h,
E..
1998.
Mod
elin
gacu
tean
dch
ron
icto
xi-
city
of
non
pola
rn
arc
oti
cch
emic
als
an
dm
ixtu
res
toC
erio
dap
hn
iad
ub
ia.
Eco
-to
xic
olo
gy
an
dE
nvir
on
men
tal
Safe
ty39:1
36-1
46
83
1059985
Acu
te(0
-96
hou
r);
Aqu
ati
c;P
lants
Lab
ra,
M.,M
att
ia,
F.,B
ern
asc
on
i,M
.,B
erta
cch
i,D
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rass
i,F
.,B
run
i,I.
,Cit
teri
o,
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Th
eC
om
bin
edT
oxic
an
dG
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xic
Eff
ects
of
Ch
rom
ium
an
dV
ola
tile
Org
an
icC
onta
min
ants
toP
seu
dokir
chn
erie
lla
sub
cap
itata
.W
ate
r,A
ir,an
dS
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on
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0
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INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
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1916722
Oth
er;
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echt,
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lain
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Rap
idb
ioass
essm
ent
met
hod
sfo
rass
essi
ng
veg
etati
on
toxic
ity
at
the
Savan
nah
Riv
erS
ite
-ger
min
ati
on
test
san
dro
ot
elon
gati
on
tria
ls.
88
2127844
Acu
te(0
-96
hou
r);
Aqu
ati
c;P
lants
Bacs
i,I.
,Toer
oek
,T
.,B
-Ber
es,
V.,T
oer
oek
,P
.,T
oth
mer
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A.S
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s,G
..2013.
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ora
tory
an
dm
icro
cosm
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erim
ents
test
ing
the
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ity
of
chlo
-ri
nate
dhyd
roca
rbon
son
acy
an
ob
act
eriu
mst
rain
(Syn
ech
oco
ccu
sP
CC
6301)
an
don
natu
ral
phyto
pla
nkto
nass
emb
lages
.H
yd
rob
iolo
gia
710:1
89-2
03
90
2298399
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Sm
ith
,A
.D
.,B
hara
th,
A.,M
allard
,C
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rr,
D.,S
mit
h,
K.,S
utt
on
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A.,V
ukm
an
ich
,J.,M
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y,L
.S
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zbu
rn,
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Th
eacu
tean
dch
ron
icto
xic
ity
of
10
chlo
rin
ate
dorg
an
ic-c
om
pou
nd
sto
the
am
eric
an
flagfi
sh(j
ord
an
ella
-flori
dae)
.A
rch
ives
of
Envir
on
men
talC
onta
min
ati
on
an
dT
oxic
olo
gy
20:9
4-1
02
93
2298399
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
Sm
ith
,A
.D
.,B
hara
th,
A.,M
allard
,C
.,O
rr,
D.,
Sm
ith
,K
.,S
utt
on
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A.,V
ukm
an
ich
,J.,M
cCart
y,L
.S
.,O
zbu
rn,
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Th
eacu
tean
dch
ron
icto
xic
ity
of
10
chlo
rin
ate
dorg
an
ic-c
om
pou
nd
sto
the
am
eric
an
flagfi
sh(j
ord
an
ella
-flori
dae)
.A
rch
ives
of
Envir
on
men
talC
onta
min
ati
on
an
dT
oxic
olo
gy
20:9
4-1
02
96
3298076
Acu
te(0
-96
hou
r);
Aqu
ati
c;P
lants
Bacs
i,I.
,Gon
da,
S.,B
-Ber
es,
V.,N
ovak,
Z.,N
agy,
S.
A.,V
asa
s,G
..2015.
Alt
er-
ati
on
sof
phyto
pla
nkto
nass
emb
lages
trea
ted
wit
hch
lori
nate
dhyd
roca
rbon
s:ef
-fe
cts
of
dom
inant
spec
ies
sen
siti
vit
yan
din
itia
ld
iver
sity
.E
coto
xic
olo
gy
24:8
23-
834
99
3559784
Acu
te(0
-96
hou
r);
Aqu
ati
c;oth
erso
ilfu
ngi
Ste
iman
,R
.,S
eigle
mu
ran
di,
F.,G
uir
au
d,P
.,B
enoit
gu
yod
,J.L
..1995.
TE
ST
ING
OF
CH
LO
RIN
AT
ED
SO
LV
EN
TS
ON
MIC
RO
FU
NG
I.E
nvir
on
men
talT
oxic
ol-
ogy
an
dW
ate
rQ
uality
10:2
83-2
85
102
3559784
Acu
te(0
-96
hou
r);
Aqu
ati
c;oth
erfu
ngi
Ste
iman
,R
.,S
eigle
mu
ran
di,
F.,G
uir
au
d,P
.,B
enoit
gu
yod
,J.L
..1995.
TE
ST
ING
OF
CH
LO
RIN
AT
ED
SO
LV
EN
TS
ON
MIC
RO
FU
NG
I.E
nvir
on
men
talT
oxic
ol-
ogy
an
dW
ate
rQ
uality
10:2
83-2
85
104
3616526
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
Loek
le,
D.
M.,S
chec
ter,
A.
J.,C
hri
stia
n,
J.
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Eff
ects
of
Ch
loro
form
,T
etra
chlo
roet
hyle
ne,
an
dT
rich
loro
ethyle
ne
on
Su
rviv
al,
Gro
wth
,an
dL
iver
of
Poec
ilia
sph
enop
s.30:1
99-2
05
106
3617731
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Horn
e,J.
D.,S
wir
sky,
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A.,H
ollis
ter,
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A.,O
bla
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R.,K
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edy,
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Aqu
ati
cT
oxic
ity
Stu
die
sof
Fiv
eP
riori
tyP
ollu
tants
.108
3617731
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te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Horn
e,J.
D.,S
wir
sky,
M.
A.,H
ollis
ter,
T.
A.,
Obla
d,
B.
R.,K
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edy,
J.
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Aqu
ati
cT
oxic
ity
Stu
die
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Fiv
eP
riori
tyP
ollu
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3617735
Acu
te(0
-96
hou
r);
Aqu
ati
c;H
ollis
ter,
T.
A.,P
ark
er,
A.
H.,
Jr.
,Parr
ish
,P
.R
..1968.
Acu
tean
dC
hro
nic
Toxic
ity
of
Fiv
eC
hem
icals
toM
ysi
dS
hri
mp
(Mysi
dop
sis
bah
ia).
112
3617735
Ch
ron
ic(>
21
days)
;A
qu
ati
c;In
-ver
teb
rate
sH
ollis
ter,
T.
A.,
Park
er,
A.
H.,
Jr.
,Parr
ish
,P
.R
..1968.
Acu
tean
dC
hro
nic
Toxic
ity
of
Fiv
eC
hem
icals
toM
ysi
dS
hri
mp
(Mysi
dop
sis
bah
ia).
114
iv
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
3617735
Oth
er;
Aqu
ati
c;In
ver
teb
rate
sH
ollis
ter,
T.
A.,P
ark
er,
A.
H.,
Jr.
,Parr
ish
,P
.R
..1968.
Acu
tean
dC
hro
nic
Toxic
ity
of
Fiv
eC
hem
icals
toM
ysi
dS
hri
mp
(Mysi
dop
sis
bah
ia).
116
3617749
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Yosh
ioka,
Y.,
Ose
,Y
.,S
ato
,T
..1986.
Corr
elati
on
of
the
Fiv
eT
est
Met
hod
sto
Ass
ess
Ch
emic
al
Toxic
ity
an
dR
elati
on
toP
hysi
cal
Pro
per
ties
.12:1
5-2
1118
3617749
Oth
er;
Aqu
ati
c;In
ver
teb
rate
sY
osh
ioka,
Y.,O
se,
Y.,S
ato
,T
..1986.
Corr
elati
on
of
the
Fiv
eT
est
Met
hod
sto
Ass
ess
Ch
emic
al
Toxic
ity
an
dR
elati
on
toP
hysi
cal
Pro
per
ties
.12:1
5-2
1121
3617749
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Yosh
ioka,
Y.,O
se,
Y.,S
ato
,T
..1986.
Corr
elati
on
of
the
Fiv
eT
est
Met
hod
sto
Ass
ess
Ch
emic
al
Toxic
ity
an
dR
elati
on
toP
hysi
cal
Pro
per
ties
.12:1
5-2
1125
3617867
Acu
te(0
-96
hou
r);
Aqu
ati
c;P
lants
Tsa
i,K
.P
.,C
hen
,C
.Y
..2007.
An
Alg
al
Toxic
ity
Data
base
of
Org
an
icT
oxi-
cants
Der
ived
by
aC
lose
d-S
yst
emT
ech
niq
ue.
Envir
on
men
tal
Toxic
olo
gy
an
dC
hem
istr
y26:1
931-1
939
128
3625336
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Shu
bat,
P.
J.,P
oir
ier,
S.
H.,K
nu
th,
M.
L.,B
rooke,
L.
T..
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Acu
teT
oxic
-it
yof
Tet
rach
loro
ethyle
ne
an
dT
etra
chlo
roet
hyle
ne
wit
hD
imet
hylf
orm
am
ide
toR
ain
bow
Tro
ut
(Salm
ogair
dn
eri)
.28
131
3625489
Oth
er;
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ati
c;F
ish
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ell,
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D.
J..
1987.
Inte
ract
ion
sof
Halo
gen
ate
dH
yd
roca
rbon
Mix
ture
sin
the
Em
bry
oof
the
Japan
ese
Med
aka
(Ory
zias
lati
pes
).134
3625621
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
De
Foe,
D.
L..
1980.
Tet
rach
loro
ethyle
ne
Bio
ass
ay
Res
ult
s.136
3634174
Ch
ron
ic(>
21
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;A
qu
ati
c;In
-ver
teb
rate
sR
ichte
r,J.
E.,P
eter
son
,S
.F
.,K
lein
er,
C.
F..
1983.
Acu
tean
dC
hro
nic
Toxic
ity
of
som
eC
hlo
rin
ate
dB
enze
nes
,C
hlo
rin
ate
dE
than
es,
an
dT
etra
chlo
roet
hyle
ne
toD
ap
hn
iam
agn
a.
12:6
79-6
84
(OE
CD
GD
ata
File)
138
3634174
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Ric
hte
r,J.
E.,P
eter
son
,S
.F
.,K
lein
er,
C.
F..
1983.
Acu
tean
dC
hro
nic
Toxic
ity
of
som
eC
hlo
rin
ate
dB
enze
nes
,C
hlo
rin
ate
dE
than
es,
an
dT
etra
chlo
roet
hyle
ne
toD
ap
hn
iam
agn
a.
12:6
79-6
84
(OE
CD
GD
ata
File)
141
3634370
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Call,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
.,R
ichte
r,J.
E..
1983.
Toxic
ity
an
dM
etab
olism
Stu
die
sw
ith
EP
A(E
nvir
on
men
talP
rote
ctio
nA
gen
cy)
Pri
ori
tyP
ol-
luta
nts
an
dR
elate
dC
hem
icals
inF
resh
wate
rO
rgan
ism
s.
144
3634370
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Call,
D.
J.,
Bro
oke,
L.
T.,A
hm
ad
,N
.,R
ichte
r,J.
E..
1983.
Toxic
ity
an
dM
etab
olism
Stu
die
sw
ith
EP
A(E
nvir
on
men
talP
rote
ctio
nA
gen
cy)
Pri
ori
tyP
ol-
luta
nts
an
dR
elate
dC
hem
icals
inF
resh
wate
rO
rgan
ism
s.
146
3634370
Acu
te(0
-96
hou
r);
Aqu
ati
c;oth
erfi
shan
din
ver
tC
all,
D.
J.,
Bro
oke,
L.
T.,A
hm
ad
,N
.,R
ichte
r,J.
E..
1983.
Toxic
ity
an
dM
etab
olism
Stu
die
sw
ith
EP
A(E
nvir
on
men
talP
rote
ctio
nA
gen
cy)
Pri
ori
tyP
ol-
luta
nts
an
dR
elate
dC
hem
icals
inF
resh
wate
rO
rgan
ism
s.
148
3634375
Ch
ron
ic(>
21
days)
;A
qu
ati
c;In
-ver
teb
rate
sC
all,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
..1980.
Toxic
ity,
Bio
con
centr
ati
on
,an
dM
etab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.150
v
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
3634375
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Call,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
..1980.
Toxic
ity,
Bio
con
centr
ati
on
,an
dM
etab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.152
3634375
Oth
er;
Aqu
ati
c;In
ver
teb
rate
sC
all,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
..1980.
Toxic
ity,
Bio
con
centr
ati
on
,an
dM
etab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.154
3634391
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Call,
D.
J.,
Bro
oke,
L.
T.,A
hm
ad
,N
..1979.
Toxic
ity,
Bio
con
centr
ati
on
an
dM
etab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.156
3634391
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Call,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
..1979.
Toxic
ity,
Bio
con
centr
ati
on
and
Met
ab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.158
3634391
Acu
te(0
-96
hou
r);
Aqu
ati
c;oth
erin
ver
t,fi
sh,a
lgae
Call,
D.
J.,B
rooke,
L.
T.,A
hm
ad
,N
..1979.
Toxic
ity,
Bio
con
centr
ati
on
and
Met
ab
olism
of
Sel
ecte
dC
hem
icals
inA
qu
ati
cO
rgan
ism
s.160
3634436
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Bro
oke,
L..
1987.
Rep
ort
of
the
Flo
w-T
hro
ugh
an
dS
tati
cA
cute
Tes
tC
om
-p
ari
son
sw
ith
Fath
ead
Min
now
san
dA
cute
Tes
tsw
ith
an
Am
ph
ipod
an
da
Cla
doce
ran
.
162
3689695
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Ah
mad
,N
.,B
enoit
,D
.,B
rooke,
L.,C
all,
D.,C
arl
son
,A
.,D
efoe,
D.,H
uot,
J.,M
ori
ari
ty,
A.,R
ichte
r,J.,S
hu
bat,
P.,
Vei
th,
G.,W
all
bri
dge,
C..
1984.
Aqu
ati
cT
oxic
ity
Tes
tsto
Ch
ara
cter
ize
the
Haza
rdof
Vola
tile
Org
an
icC
hem
icals
inW
ate
r:A
Toxic
ity
Data
Su
mm
ary
–P
art
sI
an
dII
.
164
3689695
Ch
ron
ic(>
21
days)
;A
qu
ati
c;F
ish
Ah
mad
,N
.,B
enoit
,D
.,B
rooke,
L.,C
all
,D
.,C
arl
son
,A
.,D
efoe,
D.,H
uot,
J.,M
ori
ari
ty,
A.,R
ichte
r,J.,S
hu
bat,
P.,
Vei
th,
G.,W
all
bri
dge,
C..
1984.
Aqu
ati
cT
oxic
ity
Tes
tsto
Ch
ara
cter
ize
the
Haza
rdof
Vola
tile
Org
an
icC
hem
icals
inW
ate
r:A
Toxic
ity
Data
Su
mm
ary
–P
art
sI
an
dII
.
166
3689695
;A
qu
ati
c;F
ish
Ah
mad
,N
.,B
enoit
,D
.,B
rooke,
L.,C
all,
D.,C
arl
son
,A
.,D
efoe,
D.,H
uot,
J.,M
ori
ari
ty,
A.,R
ichte
r,J.,S
hu
bat,
P.,
Vei
th,
G.,W
all
bri
dge,
C..
1984.
Aqu
ati
cT
oxic
ity
Tes
tsto
Ch
ara
cter
ize
the
Haza
rdof
Vola
tile
Org
an
icC
hem
icals
inW
ate
r:A
Toxic
ity
Data
Su
mm
ary
–P
art
sI
an
dII
.
168
4214225
Acu
te(0
-96
hou
r);
Aqu
ati
c;In
ver
-te
bra
tes
Dow
Ch
emC
o.
1979.
TO
XIC
ITY
OF
PE
RC
HL
OR
OE
TH
YL
EN
ET
OD
AP
H-
NID
S.
170
4214249
Acu
te(0
-96
hou
r);
Aqu
ati
c;F
ish
Cib
a-G
eigy
Corp
.1980.
96
HO
UR
ST
AT
ICF
ISH
BIO
AS
SA
YT
ES
TW
ITH
AT
TA
CH
ME
NT
S(A
TT
AC
HM
EN
T59).
172
vi
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
Stu
dy
Cit
ati
on:
Leb
lanc,
G.
A..
1980.
Acu
teto
xic
ity
of
pri
ori
typ
olluta
nts
tow
ate
rflea
(Daphnia
magna).
Bullet
inof
Envir
onm
enta
lC
onta
min
ati
on
and
Tox
icolo
gy
24:6
84-6
91
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;In
ver
tebra
tes
Her
oID
:7508
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
1:
Tes
tSubst
ance
Met
ric
1:
Tes
tSubst
ance
Iden
tity
Hig
h×
22
Met
ric
2:
Tes
tSubst
ance
Sourc
eL
ow×
13
study
says
all
chem
icals
test
ed
were
purc
hase
dfr
om
com
merc
ial
chem
ical
suppliers
,but
does
not
specif
yw
here
tetr
ach
loro
eth
yle
ne
cam
efr
om
.
Met
ric
3:
Tes
tSubst
ance
Puri
tyL
ow×
13
Stu
dy
rep
ort
sa
min
imum
puri
tyof
80
perc
ent
for
all
chem
icals
test
ed,
but
does
not
specif
yw
hat
the
puri
tyis
for
tetr
ach
loro
eth
yle
ne.
Dom
ain
2:
Tes
tD
esig
nM
etri
c4:
Neg
ati
ve
Contr
ols
Hig
h×
22
Met
ric
5:
Neg
ati
ve
Contr
ol
Res
ponse
Hig
h×
11
Met
ric
6:
Random
ized
Alloca
tion
Hig
h×
11
Dom
ain
3:
Exp
osu
reC
hara
cter
izati
on
Met
ric
7:
Exp
erim
enta
lSyst
em/T
est
Med
iaP
repara
-ti
on
Low
×2
6It
app
ears
the
vola
tility
of
tetr
ach
loro
eth
yle
ne
was
taken
into
account
inth
ete
stm
eth
ods,
but
it’s
un-
cle
ar
as
the
study
rep
ort
sgenera
lly
”T
he
test
sw
ere
als
oconducte
din
unre
plicate
d500
mL
solu
tions
con-
tain
ing
15
daphnid
sif
div
idin
gth
eso
luti
on
into
trip
-licate
test
vess
els
pre
sente
da
risk
of
the
loss
of
the
test
subst
ance
thro
ugh
vola
tilizati
on
or
ifvap
ors
of
the
subst
ance
pose
da
hig
hhealt
hri
skto
the
invest
i-gato
rs.
Inaddit
ion,
these
vess
els
were
covere
dw
ith
pla
stic
wra
pse
cure
dw
ith
an
ela
stic
band.”
Met
ric
8:
Consi
sten
cyof
Exp
osu
reA
dm
inis
trati
on
Med
ium
×1
2only
min
or
uncert
ain
ties
Met
ric
9:
Mea
sure
men
tof
Tes
tSubst
ance
Conce
ntr
a-
tion
Low
×1
3m
easu
rem
ents
were
not
rep
ort
ed
and
the
test
sub-
stance
isvola
tile
Met
ric
10:
Exp
osu
reD
ura
tion
and
Fre
quen
cyH
igh
×2
2M
etri
c11:
Num
ber
of
Exp
osu
reG
roups/
Spaci
ng
of
Ex-
posu
reL
evel
sM
ediu
m×
12
5-8
exp
osu
regro
ups
were
use
dfo
reach
chem
ical.
no
range
findin
gw
as
conducte
dto
dete
rmin
ean
appro
-pri
ate
exp
osu
re,
but
itapp
ears
they
were
appro
pri
-ate
enough
toest
ablish
an
LD
50.
Continued
on
nextpage...
1
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Leb
lanc,
G.
A..
1980.
Acu
teto
xic
ity
of
pri
ori
typ
olluta
nts
tow
ate
rflea
(Daphnia
magna).
Bullet
inof
Envir
onm
enta
lC
onta
min
ati
on
and
Tox
icolo
gy
24:6
84-6
91
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;In
ver
tebra
tes
Her
oID
:7508
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Met
ric
12:
Tes
ting
at
or
Bel
owSolu
bilit
yL
imit
Hig
h×
11
Dom
ain
4:
Tes
tO
rganis
mM
etri
c13:
Tes
tO
rganis
mC
hara
cter
isti
csH
igh
×2
2M
etri
c14:
Acc
lim
itiz
ati
on
and
Pre
trea
tmen
tC
ondit
ions
Low
×1
3st
udy
did
n’t
rep
ort
wheth
er
test
org
anis
ms
were
ac-
clim
ati
zed.
Met
ric
15:
Num
ber
of
Org
anis
ms
and
Rep
lica
tes
per
Gro
up
Low
×1
3It
app
ears
there
were
15
daphnia
ineach
test
con-
centr
ati
on
for
tetr
ach
loro
eth
yle
ne
and
no
replicate
sto
avoid
losi
ng
tetr
ach
loro
eth
yle
ne
tovit
alizati
on.
OE
CD
recom
mends
at
least
20
and
separa
ted
into
4diff
ere
nt
vess
els
.
Met
ric
16:
Adeq
uacy
of
Tes
tC
ondit
ions
Hig
h×
11
Dom
ain
5:
Outc
om
eA
sses
smen
tM
etri
c17:
Outc
om
eA
sses
smen
tM
ethodolo
gy
Hig
h×
22
Met
ric
18:
Consi
sten
cyof
Outc
om
eA
sses
smen
tH
igh
×1
1
Dom
ain
6:
Confo
undin
g/
Vari
able
Contr
ol
Met
ric
19:
Confo
undin
gV
ari
able
sin
Tes
tD
esig
nand
Pro
cedure
sH
igh
×2
2
Met
ric
20:
Outc
om
esU
nre
late
dto
Exp
osu
reH
igh
×1
1
Dom
ain
7:
Data
Pre
senta
tion
and
Analy
sis
Met
ric
21:
Sta
tist
ical
Met
hods
Hig
h×
11
Met
ric
22:
Rep
ort
ing
of
Data
Med
ium
×2
4D
ata
for
most
but
not
all
outc
om
es
by
study
gro
up
were
rep
ort
ed
but
these
min
or
uncert
ain
ties
or
lim
i-ta
tions
are
unlikely
tohave
asu
bst
anti
al
impact
on
resu
lts.
Met
ric
23:
Expla
nati
on
of
Unex
pec
ted
Outc
om
esH
igh
×1
1
Over
all
Quality
Det
erm
inati
on‡
Hig
h1.6
Extr
act
edY
es
Continued
on
nextpage...
2
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Leb
lanc,
G.
A..
1980.
Acu
teto
xic
ity
of
pri
ori
typ
olluta
nts
tow
ate
rflea
(Daphnia
magna).
Bullet
inof
Envir
onm
enta
lC
onta
min
ati
on
and
Tox
icolo
gy
24:6
84-6
91
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;In
ver
tebra
tes
Her
oID
:7508
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
?M
WF
=M
etri
cW
eighti
ng
Fact
or
†H
igh
=1;
Med
ium
=2;
Low
=3;
Un
acc
epta
ble
=4;
N/A
has
no
valu
e.‡
Th
eover
all
rati
ng
isca
lcu
late
das
nec
essa
ry.
EP
Am
ay
not
alw
ays
pro
vid
ea
com
men
tfo
ra
met
ric
that
has
bee
nca
tegori
zed
as
Hig
h.
Over
all
rati
ng
=
4if
any
met
ric
isU
nacc
epta
ble
⌊ ∑ i(M
etri
cS
core
i×
MW
Fi)/∑ jM
WFj
⌉ 0.1(r
ou
nd
toth
en
eare
stte
nth
)oth
erw
ise
,
wh
ere
Hig
h:≥
1to
<1.7
;M
ediu
m:≥
1.7
to<
2.3
;L
ow
:≥
2.3
to≤
3.
Ifth
ere
vie
wer
det
erm
ines
that
the
over
all
rati
ng
nee
ds
ad
just
men
t,th
eori
gin
al
rati
ng
iscr
oss
edou
tan
dan
arr
ow
poin
tsto
the
new
rati
ng.
††M
etri
csth
at
are
rate
d’H
igh
’m
etth
ecr
iter
iafo
rh
igh
con
fid
ence
as
exp
ecte
dfo
rth
isty
pe
of
stu
dy,
an
dm
ay
not
requ
ire
ad
dit
ion
al
com
men
ts.
3
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
Stu
dy
Cit
ati
on:
Bro
der
ius,
S.,K
ahl,
M..
1985.
Acu
teto
xic
ity
of
org
anic
chem
ical
mix
ture
sto
the
fath
ead
min
now
.A
quati
cT
oxic
olo
gy
6:3
07-3
22
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;F
ish
Her
oID
:12017
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
1:
Tes
tSubst
ance
Met
ric
1:
Tes
tSubst
ance
Iden
tity
Hig
h×
22
Met
ric
2:
Tes
tSubst
ance
Sourc
eH
igh
×1
1M
etri
c3:
Tes
tSubst
ance
Puri
tyH
igh
×1
1
Dom
ain
2:
Tes
tD
esig
nM
etri
c4:
Neg
ati
ve
Contr
ols
Hig
h×
22
Met
ric
5:
Neg
ati
ve
Contr
ol
Res
ponse
Hig
h×
11
Met
ric
6:
Random
ized
Alloca
tion
Hig
h×
11
Dom
ain
3:
Exp
osu
reC
hara
cter
izati
on
Met
ric
7:
Exp
erim
enta
lSyst
em/T
est
Med
iaP
repara
-ti
on
Hig
h×
22
Met
ric
8:
Consi
sten
cyof
Exp
osu
reA
dm
inis
trati
on
Hig
h×
11
Met
ric
9:
Mea
sure
men
tof
Tes
tSubst
ance
Conce
ntr
a-
tion
Hig
h×
11
Met
ric
10:
Exp
osu
reD
ura
tion
and
Fre
quen
cyH
igh
×2
2M
etri
c11:
Num
ber
of
Exp
osu
reG
roups/
Spaci
ng
of
Ex-
posu
reL
evel
sH
igh
×1
1
Met
ric
12:
Tes
ting
at
or
Bel
owSolu
bilit
yL
imit
Hig
h×
11
Dom
ain
4:
Tes
tO
rganis
mM
etri
c13:
Tes
tO
rganis
mC
hara
cter
isti
csH
igh
×2
2M
etri
c14:
Acc
lim
itiz
ati
on
and
Pre
trea
tmen
tC
ondit
ions
Hig
h×
11
Met
ric
15:
Num
ber
of
Org
anis
ms
and
Rep
lica
tes
per
Gro
up
Hig
h×
11
Met
ric
16:
Adeq
uacy
of
Tes
tC
ondit
ions
Hig
h×
11
Dom
ain
5:
Outc
om
eA
sses
smen
tM
etri
c17:
Outc
om
eA
sses
smen
tM
ethodolo
gy
Hig
h×
22
Met
ric
18:
Consi
sten
cyof
Outc
om
eA
sses
smen
tH
igh
×1
1
Continued
on
nextpage...
4
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Bro
der
ius,
S.,K
ahl,
M..
1985.
Acu
teto
xic
ity
of
org
anic
chem
ical
mix
ture
sto
the
fath
ead
min
now
.A
quati
cT
oxic
olo
gy
6:3
07-3
22
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;F
ish
Her
oID
:12017
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
6:
Confo
undin
g/
Vari
able
Contr
ol
Met
ric
19:
Confo
undin
gV
ari
able
sin
Tes
tD
esig
nand
Pro
cedure
sH
igh
×2
2
Met
ric
20:
Outc
om
esU
nre
late
dto
Exp
osu
reH
igh
×1
1
Dom
ain
7:
Data
Pre
senta
tion
and
Analy
sis
Met
ric
21:
Sta
tist
ical
Met
hods
Hig
h×
11
Met
ric
22:
Rep
ort
ing
of
Data
Hig
h×
22
Met
ric
23:
Expla
nati
on
of
Unex
pec
ted
Outc
om
esH
igh
×1
1
Over
all
Quality
Det
erm
inati
on‡
Hig
h1.0
Extr
act
edY
es
?M
WF
=M
etri
cW
eighti
ng
Fact
or
†H
igh
=1;
Med
ium
=2;
Low
=3;
Un
acc
epta
ble
=4;
N/A
has
no
valu
e.‡
Th
eover
all
rati
ng
isca
lcu
late
das
nec
essa
ry.
EP
Am
ay
not
alw
ays
pro
vid
ea
com
men
tfo
ra
met
ric
that
has
bee
nca
tegori
zed
as
Hig
h.
Over
all
rati
ng
=
4if
any
met
ric
isU
nacc
epta
ble
⌊ ∑ i(M
etri
cS
core
i×
MW
Fi)/∑ jM
WFj
⌉ 0.1(r
ou
nd
toth
en
eare
stte
nth
)oth
erw
ise
,
wh
ere
Hig
h:≥
1to
<1.7
;M
ediu
m:≥
1.7
to<
2.3
;L
ow
:≥
2.3
to≤
3.
Ifth
ere
vie
wer
det
erm
ines
that
the
over
all
rati
ng
nee
ds
ad
just
men
t,th
eori
gin
al
rati
ng
iscr
oss
edou
tan
dan
arr
ow
poin
tsto
the
new
rati
ng.
††M
etri
csth
at
are
rate
d’H
igh
’m
etth
ecr
iter
iafo
rh
igh
con
fid
ence
as
exp
ecte
dfo
rth
isty
pe
of
stu
dy,
an
dm
ay
not
requ
ire
ad
dit
ion
al
com
men
ts.
5
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
Stu
dy
Cit
ati
on:
Bro
der
ius,
S.,K
ahl,
M..
1985.
Acu
teto
xic
ity
of
org
anic
chem
ical
mix
ture
sto
the
fath
ead
min
now
.A
quati
cT
oxic
olo
gy
6:3
07-3
22
Data
Typ
e:C
hro
nic
(>21
day
s);
Aquati
c;F
ish
Her
oID
:12017
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
1:
Tes
tSubst
ance
Met
ric
1:
Tes
tSubst
ance
Iden
tity
Hig
h×
22
Met
ric
2:
Tes
tSubst
ance
Sourc
eH
igh
×1
1M
etri
c3:
Tes
tSubst
ance
Puri
tyH
igh
×1
1
Dom
ain
2:
Tes
tD
esig
nM
etri
c4:
Neg
ati
ve
Contr
ols
Hig
h×
22
Met
ric
5:
Neg
ati
ve
Contr
ol
Res
ponse
Hig
h×
11
Met
ric
6:
Random
ized
Alloca
tion
Hig
h×
11
Dom
ain
3:
Exp
osu
reC
hara
cter
izati
on
Met
ric
7:
Exp
erim
enta
lSyst
em/T
est
Med
iaP
repara
-ti
on
Hig
h×
22
Met
ric
8:
Consi
sten
cyof
Exp
osu
reA
dm
inis
trati
on
Hig
h×
11
Met
ric
9:
Mea
sure
men
tof
Tes
tSubst
ance
Conce
ntr
a-
tion
Hig
h×
11
Met
ric
10:
Exp
osu
reD
ura
tion
and
Fre
quen
cyH
igh
×2
2M
etri
c11:
Num
ber
of
Exp
osu
reG
roups/
Spaci
ng
of
Ex-
posu
reL
evel
sH
igh
×1
1
Met
ric
12:
Tes
ting
at
or
Bel
owSolu
bilit
yL
imit
Hig
h×
11
Dom
ain
4:
Tes
tO
rganis
mM
etri
c13:
Tes
tO
rganis
mC
hara
cter
isti
csH
igh
×2
2M
etri
c14:
Acc
lim
itiz
ati
on
and
Pre
trea
tmen
tC
ondit
ions
Hig
h×
11
Met
ric
15:
Num
ber
of
Org
anis
ms
and
Rep
lica
tes
per
Gro
up
Hig
h×
11
Met
ric
16:
Adeq
uacy
of
Tes
tC
ondit
ions
Hig
h×
11
Dom
ain
5:
Outc
om
eA
sses
smen
tM
etri
c17:
Outc
om
eA
sses
smen
tM
ethodolo
gy
Hig
h×
22
Met
ric
18:
Consi
sten
cyof
Outc
om
eA
sses
smen
tH
igh
×1
1
Continued
on
nextpage...
6
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Bro
der
ius,
S.,K
ahl,
M..
1985.
Acu
teto
xic
ity
of
org
anic
chem
ical
mix
ture
sto
the
fath
ead
min
now
.A
quati
cT
oxic
olo
gy
6:3
07-3
22
Data
Typ
e:C
hro
nic
(>21
day
s);
Aquati
c;F
ish
Her
oID
:12017
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
6:
Confo
undin
g/
Vari
able
Contr
ol
Met
ric
19:
Confo
undin
gV
ari
able
sin
Tes
tD
esig
nand
Pro
cedure
sH
igh
×2
2
Met
ric
20:
Outc
om
esU
nre
late
dto
Exp
osu
reH
igh
×1
1
Dom
ain
7:
Data
Pre
senta
tion
and
Analy
sis
Met
ric
21:
Sta
tist
ical
Met
hods
Hig
h×
11
Met
ric
22:
Rep
ort
ing
of
Data
Hig
h×
22
Met
ric
23:
Expla
nati
on
of
Unex
pec
ted
Outc
om
esH
igh
×1
1
Over
all
Quality
Det
erm
inati
on‡
Hig
h1.0
Extr
act
edN
o
?M
WF
=M
etri
cW
eighti
ng
Fact
or
†H
igh
=1;
Med
ium
=2;
Low
=3;
Un
acc
epta
ble
=4;
N/A
has
no
valu
e.‡
Th
eover
all
rati
ng
isca
lcu
late
das
nec
essa
ry.
EP
Am
ay
not
alw
ays
pro
vid
ea
com
men
tfo
ra
met
ric
that
has
bee
nca
tegori
zed
as
Hig
h.
Over
all
rati
ng
=
4if
any
met
ric
isU
nacc
epta
ble
⌊ ∑ i(M
etri
cS
core
i×
MW
Fi)/∑ jM
WFj
⌉ 0.1(r
ou
nd
toth
en
eare
stte
nth
)oth
erw
ise
,
wh
ere
Hig
h:≥
1to
<1.7
;M
ediu
m:≥
1.7
to<
2.3
;L
ow
:≥
2.3
to≤
3.
Ifth
ere
vie
wer
det
erm
ines
that
the
over
all
rati
ng
nee
ds
ad
just
men
t,th
eori
gin
al
rati
ng
iscr
oss
edou
tan
dan
arr
ow
poin
tsto
the
new
rati
ng.
††M
etri
csth
at
are
rate
d’H
igh
’m
etth
ecr
iter
iafo
rh
igh
con
fid
ence
as
exp
ecte
dfo
rth
isty
pe
of
stu
dy,
an
dm
ay
not
requ
ire
ad
dit
ion
al
com
men
ts.
7
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
Stu
dy
Cit
ati
on:
Barr
ows,
M.
E.,P
etro
celli,
S.
R.,M
ace
k,
K.
J.,C
arr
oll,
J.
J..
1980.
Bio
conce
ntr
ati
on
and
elim
inati
on
of
sele
cted
wate
rp
olluta
nts
by
blu
egill
sunfish
(Lep
om
ism
acr
och
irus)
.D
ata
Typ
e:C
hro
nic
(>21
day
s);
Aquati
c;F
ish
Her
oID
:18050
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
1:
Tes
tSubst
ance
Met
ric
1:
Tes
tSubst
ance
Iden
tity
Hig
h×
22
Met
ric
2:
Tes
tSubst
ance
Sourc
eH
igh
×1
1M
etri
c3:
Tes
tSubst
ance
Puri
tyL
ow×
13
no
puri
tyof
test
chem
ical
was
rep
ort
ed,
but
liquid
gas
chro
mato
gra
phy
was
perf
orm
ed
duri
ng
the
ex-
peri
ment
and
puri
tyof
the
chem
ical
could
be
dete
r-m
ined
then
alt
hough
itw
asn
’tre
port
ed
inth
epap
er.
Dom
ain
2:
Tes
tD
esig
nM
etri
c4:
Neg
ati
ve
Contr
ols
Hig
h×
22
Met
ric
5:
Neg
ati
ve
Contr
ol
Res
ponse
Hig
h×
11
Met
ric
6:
Random
ized
Alloca
tion
Low
×1
3m
eth
od
for
all
ocati
on
was
not
rep
ort
ed.
Dom
ain
3:
Exp
osu
reC
hara
cter
izati
on
Met
ric
7:
Exp
erim
enta
lSyst
em/T
est
Med
iaP
repara
-ti
on
Hig
h×
22
Met
ric
8:
Consi
sten
cyof
Exp
osu
reA
dm
inis
trati
on
Hig
h×
11
Met
ric
9:
Mea
sure
men
tof
Tes
tSubst
ance
Conce
ntr
a-
tion
Hig
h×
11
Met
ric
10:
Exp
osu
reD
ura
tion
and
Fre
quen
cyH
igh
×2
2M
etri
c11:
Num
ber
of
Exp
osu
reG
roups/
Spaci
ng
of
Ex-
posu
reL
evel
sH
igh
×1
1
Met
ric
12:
Tes
ting
at
or
Bel
owSolu
bilit
yL
imit
Hig
h×
11
Dom
ain
4:
Tes
tO
rganis
mM
etri
c13:
Tes
tO
rganis
mC
hara
cter
isti
csM
ediu
m×
24
Min
or
rese
rvati
ons
ab
out
the
sourc
eof
fish
.T
hre
ep
opula
tions
of
blu
egill
sunfish
(Lep
om
ism
acro
chir
us)
were
obta
ined
from
acom
merc
ial
fish
farm
er
inC
onnecti
cut.
May
not
all
be
the
sam
eage,
but
length
and
weig
ht
was
docum
ente
d,and
age
may
not
be
abig
facto
rin
dete
rmin
ing
BC
F.
Continued
on
nextpage...
8
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Barr
ows,
M.
E.,P
etro
celli,
S.
R.,M
ace
k,
K.
J.,C
arr
oll,
J.
J..
1980.
Bio
conce
ntr
ati
on
and
elim
inati
on
of
sele
cted
wate
rp
olluta
nts
by
blu
egill
sunfish
(Lep
om
ism
acr
och
irus)
.D
ata
Typ
e:C
hro
nic
(>21
day
s);
Aquati
c;F
ish
Her
oID
:18050
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Met
ric
14:
Acc
lim
itiz
ati
on
and
Pre
trea
tmen
tC
ondit
ions
Med
ium
×1
2F
ish
were
main
tain
ed
inth
ehold
ing
facilit
ies
for
am
inim
um
of
30
days
pri
or
toth
ein
itia
tion
of
the
study.
Min
or
uncert
ain
ties
inth
edeta
ils
pro
vid
ed.
Met
ric
15:
Num
ber
of
Org
anis
ms
and
Rep
lica
tes
per
Gro
up
Med
ium
×1
2st
udy
start
ed
wit
h100
org
anis
ms
per
exp
osu
regro
up,
and
took
fish
out
5fish
on
each
sam
pling
day.
OE
CD
recom
mends
havin
genough
tore
move
at
least
4.
Unsu
reth
enum
ber
of
replicate
s.
Met
ric
16:
Adeq
uacy
of
Tes
tC
ondit
ions
Low
×1
3re
com
mended
tem
pfo
rblu
egil
lis
20-2
5degre
es
Cand
this
study
was
conducte
dat
16
degre
es
Cw
hic
hcould
have
low
ere
dm
eta
bolism
infish
.
Dom
ain
5:
Outc
om
eA
sses
smen
tM
etri
c17:
Outc
om
eA
sses
smen
tM
ethodolo
gy
Low
×2
6B
CF
sand
half
-liv
es
were
rep
ort
ed
for
each
of
the
chem
icals
.A
ssess
ment
was
not
as
sensi
tive
as
itsh
ould
be
for
calc
ula
ting
aB
CF
-O
EC
Dre
com
-m
ends
noti
ng
ifb
oth
sexes
are
use
d,
diff
ere
nces
ingro
wth
and
lipid
conte
nt
betw
een
sexes
should
be
docum
ente
dto
be
non-s
ignifi
cant
befo
reth
est
art
of
the
exp
osu
re,
inpart
icula
rif
itis
anti
cip
ate
dth
at
pooling
of
male
and
fem
ale
fish
will
be
necess
ary
toensu
redete
cta
ble
subst
ance
concentr
ati
ons
and/or
lipid
conte
nt.
This
was
not
note
d.
Met
ric
18:
Consi
sten
cyof
Outc
om
eA
sses
smen
tM
ediu
m×
12
incom
ple
tere
port
ing
of
min
or
deta
ils
of
outc
om
eas-
sess
ment
pro
tocol
executi
on
Dom
ain
6:
Confo
undin
g/
Vari
able
Contr
ol
Met
ric
19:
Confo
undin
gV
ari
able
sin
Tes
tD
esig
nand
Pro
cedure
sL
ow×
26
OE
CD
recom
mends
noti
ng
Ifb
oth
sexes
are
use
d,
diff
ere
nces
ingro
wth
and
lipid
conte
nt
betw
een
sexes
should
be
docum
ente
dto
be
non-s
ignifi
cant
befo
reth
est
art
of
the
exp
osu
re,
inpart
icula
rif
itis
anti
c-
ipate
dth
at
pooling
of
male
and
fem
ale
fish
will
be
necess
ary
toensu
redete
cta
ble
subst
ance
concentr
a-
tions
and/or
lipid
conte
nt.
This
was
not
note
d.
Met
ric
20:
Outc
om
esU
nre
late
dto
Exp
osu
reM
ediu
m×
12
data
on
att
riti
on
and
healt
houtc
om
es
unre
late
dto
exp
osu
rew
ere
not
rep
ort
ed
for
each
study
gro
up.
Dom
ain
7:
Data
Pre
senta
tion
and
Analy
sis
Met
ric
21:
Sta
tist
ical
Met
hods
Hig
h×
11
Continued
on
nextpage...
9
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
...continued
from
pre
viouspage
Stu
dy
Cit
ati
on:
Barr
ows,
M.
E.,P
etro
celli,
S.
R.,M
ace
k,
K.
J.,C
arr
oll,
J.
J..
1980.
Bio
conce
ntr
ati
on
and
elim
inati
on
of
sele
cted
wate
rp
olluta
nts
by
blu
egill
sunfish
(Lep
om
ism
acr
och
irus)
.D
ata
Typ
e:C
hro
nic
(>21
day
s);
Aquati
c;F
ish
Her
oID
:18050
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Met
ric
22:
Rep
ort
ing
of
Data
Med
ium
×2
4N
ot
all
regre
ssio
ns,
lipid
conte
nt,
and
weig
hts
were
rep
ort
ed,
but
BC
Fs
and
half
lives
were
rep
ort
ed
for
all
chem
icals
.
Met
ric
23:
Expla
nati
on
of
Unex
pec
ted
Outc
om
esH
igh
×1
1
Over
all
Quality
Det
erm
inati
on‡
Med
ium
1.7
Extr
act
edN
o
?M
WF
=M
etri
cW
eighti
ng
Fact
or
†H
igh
=1;
Med
ium
=2;
Low
=3;
Un
acc
epta
ble
=4;
N/A
has
no
valu
e.‡
Th
eover
all
rati
ng
isca
lcu
late
das
nec
essa
ry.
EP
Am
ay
not
alw
ays
pro
vid
ea
com
men
tfo
ra
met
ric
that
has
bee
nca
tegori
zed
as
Hig
h.
Over
all
rati
ng
=
4if
any
met
ric
isU
nacc
epta
ble
⌊ ∑ i(M
etri
cS
core
i×
MW
Fi)/∑ jM
WFj
⌉ 0.1(r
ou
nd
toth
en
eare
stte
nth
)oth
erw
ise
,
wh
ere
Hig
h:≥
1to
<1.7
;M
ediu
m:≥
1.7
to<
2.3
;L
ow
:≥
2.3
to≤
3.
Ifth
ere
vie
wer
det
erm
ines
that
the
over
all
rati
ng
nee
ds
ad
just
men
t,th
eori
gin
al
rati
ng
iscr
oss
edou
tan
dan
arr
ow
poin
tsto
the
new
rati
ng.
††M
etri
csth
at
are
rate
d’H
igh
’m
etth
ecr
iter
iafo
rh
igh
con
fid
ence
as
exp
ecte
dfo
rth
isty
pe
of
stu
dy,
an
dm
ay
not
requ
ire
ad
dit
ion
al
com
men
ts.
10
INTERAGENCY DRAFT. DO NOT CITE OR QUOTE
-
Stu
dy
Cit
ati
on:
Bucc
afu
sco,
R.
J.,E
lls,
S.
J.,L
ebla
nc,
G.
A..
1981.
Acu
teto
xic
ity
of
pri
ori
typ
olluta
nts
toblu
egill
(Lep
om
ism
acr
och
irus)
.B
ullet
inof
Envir
onm
enta
lC
onta
min
ati
on
and
Tox
icolo
gy
26:4
46-4
52
Data
Typ
e:A
cute
(0-9
6hour)
;A
quati
c;F
ish
Her
oID
:18064
Dom
ain
Met
ric
Rati
ng†
MW
F?
Sco
reC
om
men
ts††
Dom
ain
1:
Tes
tSubst
ance
Met
ric
1:
Tes
tSubst
ance
Iden
tity
Hig
h×
22
Met
ric
2:
Tes
tSubst
ance
Sourc
eL
ow×
13
Test
chem
icals
,list
ed
inT
able
1,w
ere
pro
cure
dfr
om
those
com
merc
ials
ourc
es
able
topro
vid
eth
epure
st9ra
de
available