Download - Plants and the Environment
![Page 1: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/1.jpg)
Plants and the EnvironmentYang Zhong & Yufang Zheng
(School of Life Sciences, Fudan University)
![Page 2: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/2.jpg)
About the Course
• BIOL 107 Plants and the environment• Undergraduates from Pepperdine University• Shanghai, September 20 – December 6, 2010• Professors Yang Zhong and Yufang Zheng• TA: Dr Nian Liu• Textbook: Graham et al. 2006, Plant Biology (2nd
edition)• Class time: Monday 13:15-15:00; 15:30-17:00
![Page 3: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/3.jpg)
September 20: Introduction
• About the course• About the teachers• What are plants (types, ranks, names …)• Uses of plants (food, medicine, furniture …)• Cells & Molecules of life• Botany vs. Plant Biology (pure and applied botany)• Key points for the courses (biodiversity, evolution,
visit to botanical garden, DIY for specimen and DNA…)
![Page 4: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/4.jpg)
September 27: Plant Structure and Function
• Cells and cell division
• Plant structure, growth, and development
• Stems and materials transport
• Roots and plant nutrition
• Leaves and their functions
• Plant behavior
![Page 5: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/5.jpg)
October 11: Plant Reproduction and Genetics
• Plant reproduction
• Genetics and model plants
• Genetic engineering (plant breeding, GMO…)
![Page 6: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/6.jpg)
October 25: Diversity of Plants& Mid-term exam
• Various plants in the world
• Biodiversity and endangered plants
• Classification and nomenclature of plants
![Page 7: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/7.jpg)
•Understanding alive plants
•Take pictures and collect samples (for specimen and DNA) !
November 1: Visit to Shanghai Botanical Garden
![Page 8: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/8.jpg)
November 8: Biological Evolution
• Variations in plants (morphological and molecular levels)
• Phylogenetic tree and “Tree of Life”
November 15: Plant-animal Co-evolution
• What is Co-evolution?
• Case studies (e.g., butterflies and plants)
![Page 9: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/9.jpg)
November 29: Plant Ecology
• Ecology and the biosphere
• Ecosystems
• Case studies (Arid, aquatic…)
December 6: Exam
• Submission of report
• Final exam
![Page 10: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/10.jpg)
“Lab work”
1. Make specimens
2. Extract plant DNAs
![Page 11: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/11.jpg)
Requirements and Grading
• 1 report (2-3 pages) 20 pts• 1 photo show (plants in botanical garden) 10 pts• lab work 10 pts• Mid-term exam 25 pts• Final exam 35 pts
Total: 100 pts
![Page 12: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/12.jpg)
![Page 13: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/13.jpg)
![Page 14: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/14.jpg)
![Page 15: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/15.jpg)
Requirements and Grading
• 1 report (2-3 pages) 20 pts• 1 photo show (plants in botanical garden) 10 pts• lab work 10 pts• Mid-term exam 25 pts• Final exam 35 pts
Total: 100 pts
![Page 16: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/16.jpg)
About the Teachers
Yang Zhong ( 钟 扬 )
B. E., Electronic Engineering (The Gifted Young Class), University of Science and Technology of China
Ph. D., Biology, The Graduate University for Advanced Studies, Japan
Professor, School of Life Sciences, Fudan University, Shanghai
Director, Institute of Biodiversity Science and Geobiology, Tibet University, Lhasa
Research Professor, Wuhan Institute of Botany, Academia Sinica
Visiting Scholar, University of California-Berkeley and Michigan State University
Research interests: Mangrove ecosystem (aquatic plants), Tibetan plants, Molecular evolution, Bioinformatics
![Page 17: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/17.jpg)
About the Teachers
Yufang Zheng ( 郑煜芳 )
• B. S. & M.S., Biophysics, Tsinghua University, Beijing, China
• Ph. D., Physiology & Biophysics, Graduate School of Weill Medical College of Cornell University, NY, USA
• Associate Professor, School of Life Sciences, Fudan University, Shanghai
• Research interests: Brain development
![Page 18: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/18.jpg)
Mangroves: important ecological plants
“A Forest in the Sea”
Total 24 families, 30 genera, and about 74 species, distributed from tropical to subtropical coastal regions worldwide
China: Guangdong, Hainan, Taiwan, Hong Kong, Fujian …
USA: Florida
![Page 19: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/19.jpg)
Significant adaptive characters of mangroves
The highly adapted plants growing in coastal ecosystems
vivipary
salinityroot system
![Page 20: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/20.jpg)
Hainan Island
Field-investigation
![Page 21: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/21.jpg)
Okinawa, Japan
![Page 22: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/22.jpg)
Tibet
![Page 23: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/23.jpg)
A way for studying science
![Page 24: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/24.jpg)
Lotus ( 莲)(Nelumbo nucifera)
Waterlily (睡莲)(Nymphaea tetragona)
Stick out of water Floating leaves
Names of Plants
![Page 25: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/25.jpg)
Magnolia denudata
白玉兰
Shanghai City Flower / Tree
![Page 26: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/26.jpg)
California State Flower
![Page 27: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/27.jpg)
The California redwood (Seq
uoia sempervirens, Sequoia g
igantea) is the official state tr
ee
California State Tree
![Page 28: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/28.jpg)
Binomial nomenclature
Karl von Linne or Carl von Linné or Carolus Linnaeus (1707–1778), a Swedish botanist, invented the modern system of binomial nomenclature.
![Page 29: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/29.jpg)
Taxonomy: Hierarchy
• Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Proteales; Nelumbonaceae; Nelumbo
• Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; basal Magnoliophyta; Nymphaeales; Nymphaeaceae; Nymphaea
![Page 30: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/30.jpg)
Zhong et al., 1996, Taxon (following International Code of Plant Nomenclature)
![Page 31: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/31.jpg)
Tree of Life
![Page 32: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/32.jpg)
Three domains of “Tree of Life”The Three-Domain Hypothesis is the idea that life can be divided into three distinct (monophyletic) domains: archaebacteria, bacteria, and eukaryotes. An important part of the hypothesis is that eukaryotes are descended from ancient archaebacteria.
![Page 33: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/33.jpg)
Doolittle's Scientific American article "Uprooting the Tree of Life" (February 2000).
© Scientific American
![Page 34: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/34.jpg)
Placental mammals
![Page 35: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/35.jpg)
Pegasoferae, an unexpected mammalian clade revealed by tracking ancient retroposon insertions
from Proceedings of National Academy of Sciences of the United States, 2006
![Page 36: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/36.jpg)
Understanding plants
• Structure and function:• Molecules --- Molecular biology• Cell --- Cell biology (cytobiology)• Photosynthesis/growth… --- Plant physiology• Reproduction --- Genetics/Developmental biology• Variation/plant-animal relation --- Evolutionary biology• Name and classification --- Taxonomy • Plant-environment relation --- Ecology• Others, e.g., endangered species --- Conservation biology• e.g., diseases --- Pathology
![Page 37: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/37.jpg)
Cells
• All life-forms are composed of cells
• The eukaryotic cells of algae, protozoa, fungi, plants, and animals are more structurally complex than prokaryotic cells
![Page 38: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/38.jpg)
A Plant Cell
![Page 39: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/39.jpg)
mtDNA
![Page 40: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/40.jpg)
![Page 42: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/42.jpg)
Prokaryotes Eukaryotes
Typical organisms bacteria, archaea protists, fungi, plants, animals
Typical size ~ 1-10 µm ~ 10-100 µm (sperm cells, apart from the tail, are smaller)
Type of nucleusnucleoid region; no real
nucleusreal nucleus with double membrane
DNA circular (usually) linear molecules (chromosomes) with histone proteins
RNA-/protein-synthesis
coupled in cytoplasmRNA-synthesis inside the nucleus
protein synthesis in cytoplasm
Ribosomes 50S+30S 60S+40S
Cytoplasmatic structure
very few structures highly structured by endomembranes and a cytoskeleton
Cell movement flagella made of flagellinflagella and cilia containing microtubules; lamellipodia and filopodi
a containing actin
Mitochondria none one to several thousand (though some lack mitochondria)
Chloroplasts none in algae and plants
Organization usually single cellssingle cells, colonies, higher multicellular organisms with specialize
d cells
Cell divisionBinary fission (simple
division)Mitosis (fission or budding)Meiosis
![Page 43: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/43.jpg)
Cell Division
• All organisms are composed of one or more cells; all cells pass through the cell cycle
• Cell cycle and cell division are defining attributes of life
• Prokaryotic organisms and cell organelles reproduce by binary fission
• Eukaryotic organisms have separate processes of nuclear division and cytoplasmic division
![Page 44: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/44.jpg)
![Page 46: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/46.jpg)
![Page 47: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/47.jpg)
Electron MicroscopesTransmission Electron Microscope (TEM): 0.5 nm
Scanning Electron Microscope (SEM): less than 1 nm ~ 20 nm
Scanning Transmission Electron Microscope (STEM)
A scanning transmission electron microscope (STEM) is a type of transmission electron microscope. With it, the electrons pass through the specimen, but, as in scanning electron microscopy, the electron optics focus the beam into a narrow spot which is scanned over the sample in a raster. The STEM rasters a focused incident probe across a specimen that (as with the TEM) has been thinned to facilitate detection of electrons scattered through the specimen. The high resolution of the TEM is thus possible in STEM. The focusing action (and aberrations) occur before the electrons hit the specimen in the STEM, but afterward in the TEM. The STEM's use of SEM-like beam rastering simplifies annular dark-field imaging, and other analytical techniques, but also means that image data is acquired in serial rather than in parallel fashion.
![Page 48: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/48.jpg)
Scanning Electron Microscope (1 - 20 nm)
pollen grains from Helianthus annuus
![Page 49: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/49.jpg)
Transmission Electron Microscope (0.5 nm)
transverse section through a diatom, Phaeodactylum tricornutum. (a silicon algae )
![Page 50: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/50.jpg)
Usage of Plants
• Food, energy, construction, decoration, etc
• Sources of starch
• Sources of sugar
• Sources of oils
• Sources of oxygen
![Page 51: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/51.jpg)
• Photosynthesis provides the food and fuel
that power life on Earth
• The interaction between light and pigments
is crucial to the capture of solar energy
![Page 52: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/52.jpg)
Path of energy flow
![Page 53: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/53.jpg)
Photosynthesis and respiration• Life on Earth is solar powered• Photosynthesis is the process by which cells trap t
he energy in sunlight as chemical bonds in simple sugars
• Plant cells and those of other photosynetic organisms construct the complex carbohydrates, lipids, nucleic acids, and proteins of life from simple sugars
• Respiration is a series of chemical reactions that release energy from chemical bonds in the organic molecules which were produced by photosynthesis
![Page 54: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/54.jpg)
Flaming Mountain at Turfan (Turpan) , Xin Jiang
(>47.8 °C, Gound surface >70 °C)
![Page 55: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/55.jpg)
Grape valley
![Page 56: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/56.jpg)
A sunny day in Tibet
![Page 57: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/57.jpg)
Plants in Tibet
![Page 58: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/58.jpg)
Flowers in Tibet
![Page 59: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/59.jpg)
Lhasa, the city of sunshine (over 3000 hours per year)
![Page 60: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/60.jpg)
Silicon boards for solar energy (Lhasa)
![Page 61: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/61.jpg)
Chloroplast
![Page 62: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/62.jpg)
Photosynthesis
![Page 63: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/63.jpg)
Cooperation between two photosystems
![Page 64: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/64.jpg)
Calvin cycle: The C3
pathway
![Page 65: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/65.jpg)
Photorespiration
![Page 66: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/66.jpg)
The C4 pathway
![Page 67: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/67.jpg)
![Page 68: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/68.jpg)
Examples of CAM plants (a) Snake plant (Sansevieria) and (b) Crassula rupestris, a stonecrop
![Page 69: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/69.jpg)
![Page 70: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/70.jpg)
Respiration and fermentation
release energy for cellular metabolism
![Page 71: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/71.jpg)
![Page 72: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/72.jpg)
Beers in Shanghai
![Page 73: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/73.jpg)
Brewing Beers
• Malt
• Hops
• Yeast
• Water
![Page 74: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/74.jpg)
Barley
Yunnan Yuanyang Rice Terraces
The Botany of Beer
• Malt provided by barley, rice, …
• (Starch -> sugar -> alcohol …)
![Page 75: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/75.jpg)
Hops: flowers from Humulus species (Cannabaceae family, hemp family)
HopsHumulus lupulus
![Page 76: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/76.jpg)
Sources of starchMajor: rice, wheat, zea, potato, barley, oat, rye, sorghum
Additional: Jerusalem artichoke, taro, sweet potato, water chestnut, lily, kudzu
![Page 77: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/77.jpg)
Sources of sugar
• Major: sugarcane, sugar beet
![Page 78: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/78.jpg)
Additional: Luo-han-guo
Distributed in Guangxi
themogrosides are about 300 times sweeter than sugar
![Page 79: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/79.jpg)
Sources of oils
• Major: oil rape, peanut, sunflower, soybean
• Additional: olive oil, camellia (tea) oil, cotton seed oil
![Page 80: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/80.jpg)
Bio-energyCorn
![Page 81: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/81.jpg)
Bio-energySugarcane (Rum)
![Page 82: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/82.jpg)
Gardens in Suzhou
![Page 83: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/83.jpg)
Decoration: Primula
![Page 84: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/84.jpg)
Herbal remedy
St John's wort, is the plant species Hypericum perforatum, and is widely known as an herbal treatment for depression.
![Page 85: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/85.jpg)
Have a rest
![Page 86: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/86.jpg)
Molecules of Life
![Page 87: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/87.jpg)
Molecules of Life• Chemical elements
– 1) All physical matter is composed of chemical elements;
– 2) An element is a substance composed of only one kind of atom;
– 3) Over 90 elements occur in nature (from hydrogen, the lightest to uranium, the heaviest)
• Macronutrients– Nine elements account for 99.95% of the dry weight of
plants • Micronutrients
– trace elements (required in very small amount)
![Page 88: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/88.jpg)
Essential Elements
Organic fertilizers
• N: fabaceous herbs and muck;
• P: phosphate rock;• K: plant ash;• Ca: limestone.
![Page 89: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/89.jpg)
Left: Soybean (Glycine max), one of major nitrogen-fixing plants;
Right: Rhizobia (Rhizobium leguminosarum)
![Page 90: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/90.jpg)
Sea Buckthorn (Hippophae rhamnoides),
one of non-legume nitrogen fixing plants
Frankia is a genus of nitrogen fixing filam
entous bacteria that live in symbiosis with
actinorhizal plants, similar to Rhizobia. Ba
cteria of this genus form root nodules
![Page 91: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/91.jpg)
Nauru
Between Hawaii and Australia
22 km2
Population size about 12,000
“Country of phosphate” (38, 000, 000 tons)
From rich to poor
![Page 92: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/92.jpg)
Karst (limestone)
Stone Forest Yangshuo
Mining area
![Page 93: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/93.jpg)
Organic molecules: presence of the element carbon
• Carbohydrates (Sugars, starches, cellulose…)• Lipids (fats, oils, waxes…)• Proteins (large molecules compounds of amino
acids)• Nucleic acids (DNA, RNA…)
Four types of primary compounds
![Page 94: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/94.jpg)
![Page 95: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/95.jpg)
Molecules produced by plants, algae, or fungi that are not found in all species
• Terpenes (e.g., rose extracts)
• Phenolics (e.g., vanilla extracts)
• Flavonoids (pigments)
• Alkaloids (e.g., nicotine from tabacco)
Secondary compounds
![Page 96: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/96.jpg)
Damascan rose (Rosa damascene)
![Page 97: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/97.jpg)
Camphor tree (Cinnamomum camphora)
![Page 98: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/98.jpg)
It was first introduced to Britain from Yichang County, Hubei Province, China in 1880 and rapidly became established as a popular ornamental plant throughout Europe and the United States as well as other countries allergic contact dermatitis
An example: Primula
![Page 99: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/99.jpg)
Detection of the chemical compounds
![Page 100: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/100.jpg)
A significant cause of allergic contact dermatitis ?
In a number of sensitization experiments, 2-methoxy-6-alkyl-1,4-b
enzoquinones with branch side chain length C1-C15, including primin,
have showed an increase of the sensitizing capacity with increasing le
ngth of the alkyl side chain from C-1 to C-13 ( reaching maximum acti
vity at an alkyl chain of 10 to 11 carbons), but the sensitizing potency
decreased beyond 13 carbons and the sensitization hardly occurred at
C-1 to C-3.
![Page 101: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/101.jpg)
DNA : Genetic material
Watson and Crick
(1953)
![Page 102: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/102.jpg)
Chromosome
![Page 103: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/103.jpg)
DNA Sequence
Arabidopsis thaliana APP mRNA, complete cds aaagctttca agggaagcca tcgatgaaga agaaaacgaa gaagaagact cttcaaatgc tcgcgcgaac tcacttctga cgaaa
accat acttcctcag tctcattccc tttccgacga actattctcc tgaagaagaa gacgaaaatg gcgaacaagc tcaaagtcga cgaactccgt ttaaaactcg ccgagcgtgg actcagtact actggagtca aagccgttct ggtggagagg cttgaagagg ctatcgcaga agacactaag aaggaagaat caaagagcaa gaggaaaaga aattcttcta atgatactta tgaatcgaac aaattgattg caattggcga atttcgtggg atgattgtga aggaattgcg tgaggaagct attaagagag gcttagatac aacaggaacc aaaaaggatc ttcttgagag gctttgcaat gatgctaata acgtttccaa tgcaccagtc aaatccagta atgggacaga tgaagctgaa gatgacaaca atggctttga agaagaaaag aaagaagaga aaatcgtaac cgcgacaaag aagggtgcag cggtgttaga tcagtggatt cctgatgaga taaagagtca gtaccatgtt ctacaaaggg gtgatgatgt ttatgatgct atcttaaatc agacaaatgt cagggataat aataacaagt tctttgtcct acaagtccta gagtcggata gtaaaaagac atacatggtt tacaccagat ggggaagagt tggtgtgaaa ggacaaagta agctagatgg gccttatgac tcatgggatc gtgcgataga gatatttacc aataagttca atgacaagac aaagaattat tggtctgaca gaaaggagtt tatcccacat cccaagtcct atacatggct cgaaatggat tacggaaaag aggaaaatga ttcaccggtc aataatgata ttccgagttc atcttccgaa gttaaacctg aacaatcaaa actagatact cgggttgcca agttcatctc tcttatatgt aatgtcagca tgatggcaca gcatatgatg gaaataggat ataacgctaa caaattgcca ctcggcaaga taagcaagtc cacaatttca aagggttatg aagtgctgaa gagaatatcg gaggtgattg atcggtatga tagaacgagg cttgaggaac tgagtggaga gttctacaca gtgatacctc atgattttgg ttttaagaaa atgagccagt ttgttataga cactcctcaa aagttgaaac agaaaattga aatggttgaa gcattaggtg agattgaact cgcaacaaag ttgttgtccg tcgacccggg attgcaggat gatcctttat attatcacta ccagcaactt aattgtggtt tgacgccagt aggaaatgat tcagaggagt tctctatggt tgctaattac atggagaaca ctcatgcaaa gacgcattcg ggatatacgg ttgagattgc tcaactattt agagcttcga gagctgttga agctgatcga ttccaacagt tttcaagttc gaagaacagg atgctactct ggcacggttc acgtctcact aactgggctg gtattttatc tcaaggtctg cgaatagctc ctcctgaagc gcctgtaact ggttacatgt ttggaaaagg ggtttacttt gcggatatgt tctccaagag tgcgaactat tgctatgcca acactggcgc taatgatggc gttctgctcc tctgcgaggt tgctttggga gacatgaatg aacttctgta ttcagattat aacgcggata atctaccccc gggaaagcta agcacaaaag gtgtggggaa aacagcacca aacccatcag aggctcaaac actagaagac ggtgttgttg ttccacttgg caaaccagtg gaacgttcat gctccaaggg gatgttgttg tacaacgaat atatagtcta caatgtggaa caaatcaaga tgcgttatgt gatccaagtc aaattcaact acaagcacta aaacttatgt atattagctt ttgaacatca actaattatc caaaaatcag cgttttattg tatttctttc aaactccttc atctctgatt ttgcacggtt cactcg
![Page 104: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/104.jpg)
Arabidopsis is native to Europe, Asia, and northwestern Africa. It is an annual (rarely biennial) plant usually growing to 20–25 cm tall. The flowers are 3 mm in diameter. The fruit is a siliqua 5–20 mm long, containing 20–30 seeds.
Brassicacaea family
![Page 105: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/105.jpg)
Use as a model organism
• Arabidopsis is widely used as one of the model organisms for studying plant sciences, including genetics and plant development. It plays the role for agricultural sciences that mice and fruit flies (Drosophila) play in animal biology. Although Arabidopsis thaliana has little direct significance for agriculture, it has several traits that make it a useful model for understanding the genetic, cellular, and molecular biology of flowering plants.
• The small size of its genome make Arabidopsis thaliana useful for genetic mapping and sequencing — with about 157 million base pairs and five chromosomes, Arabidopsis has one of the smallest genomes among plants. It was the first plant genome to be sequenced, completed in 2000 by the Arabidopsis Genome Initiative. Much work has been done to assign functions to its 27,000 genes and the 35,000 proteins they encode.
![Page 106: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/106.jpg)
• The plant's small size and rapid life cycle are also advantageous for research. Having specialized as a spring ephemeral, it has been used to found several laboratory strains that take about six weeks from germination to mature seed. The small size of the plant is convenient for cultivation in a small space and it produces many seeds. Further, the selfing nature of this plant assists genetic experiments. Also, as an individual plant can produce several thousand seeds, each of the above criteria leads to Arabidopsis thaliana being valued as a genetic model organism.
• Plant transformation in Arabidopsis is routine, using Agrobacterium tumefaciens to transfer DNA to the plant genome. The current protocol, termed "floral-dip", involves simply dipping a flower into a solution containing Agrobacterium, the DNA of interest, and a detergent. This method avoids the need for tissue culture or plant regeneration.
![Page 107: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/107.jpg)
• Genetic Information is expressed through Nucleic Acids chains called RiboNucleic Acids.
• Three types of RNAs differing in size, function and localization:
• Messenger RNA (mRNA) is a carrier of genetic information, a copy of a gene sequence acting as a template for protein construction.
• Ribosomal RNA (rRNA) and Transfer RNA (tRNA) (also sometimes referred to as insoluble and soluble RNAs) are structural ribonucleic acids which support the expression of mRNA into protein.
RNA: Genetic material transformation
![Page 108: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/108.jpg)
Crick’s central dogma
![Page 109: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/109.jpg)
Genetic code
• In 1968, Nirenberg & Khorana jointly were awarded the Nobel Prize for the elucidation of the Genetic Code.
• Most codons for a given amino acid differ only in the third base of the triplet (exceptions: Leu, Arg, Ser)
• One codon (AUG or Met) also signals the START of a polypeptide chain. • Three codons (UAA, UAG and UGA) are used to signal the END of a polypeptide chain (STOP
codons)
![Page 110: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/110.jpg)
![Page 111: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/111.jpg)
DNA fingerprinting
• The chemical structure of everyone's DNA is the same. The only difference between people (or any animal/plant) is the order of the base pairs. There are so many millions of base pairs in each person's DNA that every person has a different sequence.
• Using these sequences, every person could be identified solely by the sequence of their base pairs. However, because there are so many millions of base pairs, the task would be very time-consuming. Instead, scientists are able to use a shorter method, because of repeating patterns in DNA.
• These patterns do not, however, give an individual "fingerprint," but they are able to determine whether two DNA samples are from the same person, related people, or non-related people. Scientists use a small number of sequences of DNA that are known to vary among individuals a great deal, and analyze those to get a certain probability of a match.
![Page 112: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/112.jpg)
Variable Number Tandem Repeats (VNTRs)
![Page 113: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/113.jpg)
Gel electrophoresis
![Page 114: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/114.jpg)
An example of DNA fingerprinting
![Page 115: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/115.jpg)
![Page 116: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/116.jpg)
Practical Applications of DNA Fingerprinting
1. Paternity and Maternity: Because a person inherits his or her VNTRs from his or her parents, VNTR patterns can be used to establish paternity and maternity. The patterns are so specific that a parental VNTR pattern can be reconstructed even if only the children's VNTR patterns are known (the more children produced, the more reliable the reconstruction). Parent-child VNTR pattern analysis has been used to solve standard father-identification cases as well as more complicated cases of confirming legal nationality and, in instances of adoption, biological parenthood.
2. Criminal Identification and Forensics: DNA isolated from blood, hair, skin cells, or other genetic evidence left at the scene of a crime can be compared, through VNTR patterns, with the DNA of a criminal suspect to determine guilt or innocence. VNTR patterns are also useful in establishing the identity of a homicide victim, either from DNA found as evidence or from the body itself.
3. Personal Identification : The notion of using DNA fingerprints as a sort of genetic bar code to identify individuals has been discussed, but this is not likely to happen anytime in the foreseeable future. The technology required to isolate, keep on file, and then analyze millions of very specified VNTR patterns is both expensive and impractical. Social security numbers, picture ID, and other more mundane methods are much more likely to remain the prevalent ways to establish personal identification.
![Page 117: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/117.jpg)
Problems with DNA Fingerprinting
Like nearly everything else in the scientific world, nothing about DNA fingerprinting is 100% assured. The term DNA fingerprint is, in one sense, a misnomer: it implies that, like a fingerprint, the VNTR pattern for a given person is utterly and completely unique to that person. Actually, all that a VNTR pattern can do is present a probability that the person in question is indeed the person to whom the VNTR pattern (of the child, the criminal evidence, or whatever else) belongs. Given, that probability might be 1 in 20 billion, which would indicate that the person can be reasonably matched with the DNA fingerprint; then again, that probability might only be 1 in 20, leaving a large amount of doubt regarding the specific identity of the VNTR pattern's owner.
• 1. Generating a High Probability
The probability of a DNA fingerprint belonging to a specific person needs to be reasonably high--especially in criminal cases, where the association helps establish a suspect's guilt or innocence.
![Page 118: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/118.jpg)
2. Problems with Determining Probability A. Population Genetics
VNTRs, because they are results of genetic inheritance, are not distributed evenly across all of human population. A given VNTR cannot, therefore, have a stable probability of occurrence; it will vary depending on an individual's genetic background. The difference in probabilities is particularly visible across racial lines. Some VNTRs that occur very frequently among Hispanics will occur very rarely among Caucasians or African-Americans. Currently, not enough is known about the VNTR frequency distributions among ethnic groups to determine accurate probabilities for individuals within those groups; the heterogeneous genetic composition of interracial individuals, who are growing in number, presents an entirely new set of questions. Further experimentation in this area, known as population genetics, has been surrounded with and hindered by controversy, because the idea of identifying people through genetic anomalies along racial lines comes alarmingly close to the eugenics and ethnic purification movements of the recent past, and, some argue, could provide a scientific basis for racial discrimination.
B. Technical Difficulties Errors in the hybridization and probing process must also be figured into the probability, and often the idea of error is simply not acceptable. Most people will agree that an innocent person should not be sent to jail, a guilty person allowed to walk free, or a biological mother denied her legal right to custody of her children, simply because a lab technician did not conduct an experiment accurately. When the DNA sample available is minuscule, this is an important consideration, because there is not much room for error, especially if the analysis of the DNA sample involves amplification of the sample (creating a much larger sample of genetically identical DNA from what little material is available), because if the wrong DNA is amplified (i.e. a skin cell from the lab technician) the consequences can be profoundly detrimental. Until recently, the standards for determining DNA fingerprinting matches, and for laboratory security and accuracy which would minimize error, were neither stringent nor universally codified, causing a great deal of public outcry.
![Page 119: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/119.jpg)
DNA sequence changes
![Page 120: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/120.jpg)
Ancient gene
X Y
t+ 1
t
time
X’ Y’
![Page 121: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/121.jpg)
DNA sequence changes
AAGACTT
TGGACTTAAGGCCT
-3 mil yrs
-2 mil yrs
-1 mil yrs
today
AGGGCAT TAGCCCT AGCACTT
AAGGCCT TGGACTT
TAGCCCA TAGACTT AGCGCTTAGCACAAAGGGCAT
AGGGCAT TAGCCCT AGCACTT
AAGACTT
TGGACTTAAGGCCT
AGGGCAT TAGCCCT AGCACTT
AAGGCCT TGGACTT
AGCGCTTAGCACAATAGACTTTAGCCCAAGGGCAT
![Page 122: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/122.jpg)
Splits tree analysis of 44 manuscripts of “The Wife of Bath’s Prologue” from Chaucer’s The Canterbury Tales. The two- or three-character codes indicate individual manuscripts, whereas the large capitals indicate groups of manuscripts, which are colored the same.
![Page 123: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/123.jpg)
NCBI
National Center for Biotechnology Information
– http://www.ncbi.nlm.nih.gov/
The largest DNA sequence database and bioinformatic
s center
![Page 124: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/124.jpg)
NCBI homepage
![Page 125: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/125.jpg)
![Page 126: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/126.jpg)
Fossil specimens (>10 mya)(a)Magnolia latahensis(b) Persea pseudocarolinensisThe fossil DNA sequencing was publi
shed in Nature (1990)
Ancient DNA (from herbarium/museum or fossil samples)
![Page 127: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/127.jpg)
![Page 128: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/128.jpg)
Mam-moth (Mammuthus primigenius) body from Siberian, Russia (9,000 yr)
![Page 129: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/129.jpg)
The whole mt genome of Neanderthals has been reconstructed in 2007
Neanderthals (200,000 yr ago in Europe)
![Page 130: Plants and the Environment](https://reader037.vdocuments.site/reader037/viewer/2022110118/5550327db4c9058f2f8b4fc4/html5/thumbnails/130.jpg)
Two major challenges for ancient DNA sequencing:
• DNA quality (can be sequenced after 100,000 yrs or longer, such as 10 million years) (maintenance in natural conditions)?
• Pollution (by bacteria, fungi, …)