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MLL Studies at Minot State University
Progress report for North Dakota INBRE
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Projects
• MLL translocation mechanism. What is the role of DNA topoisomerase II in MLL translocations?• In vitro cleavage assays• DNA binding assays (ChIP)
• Targeting the MLL-fusion messages by RNA interference
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INBRE GOALS
• Establishing a sustainable research program
• Creating networks with UND, NDSU, baccalaureate institutions and tribal colleges
• Preparing undergraduates for post graduate studies and careers in biomedical science
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Establishing a sustainable research program
• State of the art cell and molecular biology lab at MiSU• Cell culture facility• Fluorescence microscope• Plate reader• DNA analysis facility
• Researchers/Staff• Cheryl Lepp and Karen Pocha-Melby• 7 former/current research students/3
summer students
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Networks
• Proteomics Core– Consultation on identification of DNA binding
protein (spring 2005) InSu Hahn
• Biology CoreConsultation with the sequencing facility (spring 2005)
Real-time PCR analysis of RNA interference samples (January 2006) Sheri Dorsam
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Networks
Tribal colleges
• Turtle Mountain Community College• Visit to Minot State University, February 2006• TMCC research conference, April 2006• Lyle Best /Melanaie Nadeau-- research discussions
• Fort Berthold Community College• Kerry Hartman
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Non-INBRE colleagues
• MLL research group at University of Chicago
• Cytogenetic analysis of cell line• Consultation
• MLL research group at Loyola University• Methods and research consultation• Grant proposal consultation
• Topoisomerase II expert at University of Central Florida and consultant for Topogen Inc.
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The students
• Involving 3-4 students /semester in independent research projects
• Total of 10 since BRIN/INBRE initiated• Complete experience
– Literature– Experimental design– Analysis– Dissemination (meetings, presentations,
posters)
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Presentations
• In vitro cleavage of the MLL breakpoint cluster region by topoisomerase II. 2005 ND Academy of Science Undergraduate Research prestentation. Shelly Reinholdt.
• Studies of topo II binding in the MLL translocation breakpoint region. INBRE poster session 2005 Stephanie Mueller
• Does DNA topoisomerase II bind in the MLL translocation breakpoint region. Undergraduate Research prestentation. Stephanie Mueller
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Where are they now?
• Jessical Townsend– University of Wisconsin Madison– Ph.D. program in molecular pharmacology
• Jessica HamiltonUniversity of North Carolina Vet school
• Tonia SundlovUniversity of Wisconsin, Madison
--Ph.D. in Cancer genetics
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Plans for the Future
• Stay the course…
• Reinforce collaborations—Sheri Dorsam, MLL research collaborators
• Establish new partnerships, especially with tribal college faculty and students
• Grant proposal