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Lectures by
John F. Allen
School of Biological and Chemical Sciences, Queen Mary, University of London
Membrane Biochemistry
1
jfallen.org/lectures
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The Endomembrane System
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The Endomembrane System
Exocytosis and endocytosis
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Vesicular transport
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Vesicular transport
Three types of coated vesicles
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Vesicular transport
Three types of coated vesicles
COP I
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Vesicular transport
Three types of coated vesicles
COP ICOP II
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Vesicular transport
Three types of coated vesicles
COP ICOP II
Clathrin-coated vesicles
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Assembly of a COP II-coated vesicle
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Assembly of a COP II-coated vesicle
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Role of the COP II coat protein in generating membrane curvature, assembling the protein coat, and capturing cargo
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Role of the COP II coat protein in generating membrane curvature, assembling the protein coat, and capturing cargo
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Role of the COP II coat protein in generating membrane curvature, assembling the protein coat, and capturing cargo
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Role of the COP II coat protein in generating membrane curvature, assembling the protein coat, and capturing cargo
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Schematic view of a single Sec23-Sec24-Sar1 complex at the surface of a vesicle 60 nm in diameter
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Sorting proteins in the trans Golgi network (TGN)
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Lysosomal enzymes are recognised by N-acetylglucosamine phosphotranserase
Targeting lysosomal enzymes to lysosomes
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Targeting lysosomal enzymes to lysosomes
Path from synthesis of lysosomal enzymes on ribosomes on the Rough ER to delivery to a lysosome
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Clathrin coated vesicles
Formation of clathrin coated vesicles at the TGN
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Clathrin coated vesicles
Molecular organisation
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Receptor-mediated endocytosis
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Coated pits
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Coated pits
Extracellular surface
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Coated pits
Extracellular surface Cytosolic surface
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Reconstruction of a clathrin cage containing 36 triskelions
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The endocytotic
pathway
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Lysosomes
...and autophagy
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Next lecture...
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Membrane transport
Next lecture...
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Lectures by
John F. Allen
School of Biological and Chemical Sciences, Queen Mary, University of London
Membrane Biochemistry
1
jfallen.org/lectures
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http://en.wikipedia.org/wiki/File:Nucleus_ER_golgi.svg
Wikipedia contributors. "Golgi apparatus." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 25 Oct. 2010. Web. 25 Oct. 2010.