Jyrki Törnvall, Mesimäki, Risto Kontio

Jaw bone defect – Soft tissue defect

Preop

May 07

ASCs,ßTCP,BMP 2

Suomen Kuvalehti

In the OR In the Lab

2) Fat is broken to 1-cell suspensionand stem cells extracted

3) Stem cells and expanded to abt 1 million

4) Cells are combined with biomaterial and GF is added

5) The combination is placed in a titanium cage

1) 1dl fat containing 100 000 stem cells

6) The construct is placed in a prepared muscle pouch

7) The TRAM-flap combination is raised and transplanted to the defect site. It is rigidly fixed with titanium screws.

From FAT to bone in few months

October 07

October 07

December 07

March 08

October 08

1) Harvest adipose tissue

3) Combine cells with growth

factor BMP-2 and biomaterial

*

*

*

*

* *

*

= growth factor*= biomaterial

2) Isolate and culture

adipose stem cells (10 days

)

4) The mix is placed directly

into the patients defect site

= adipose tissue

Ameloblastoma treatment using adipose stem cells and biomaterials