![Page 1: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/1.jpg)
Introduction to Glioblastoma
Chris PlaisierIntroduction to Systems Biology Course
Institute for Systems Biology
![Page 2: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/2.jpg)
Systems Level Integration
• Integration of high-throughput technologies is a common practice in systems biology
• In these tutorial sections we will explain and then have a hands-on time to get experience working with high-throughput data
• Tutorials will focus on the deadly brain cancer glioblastoma multiforme or glioma
![Page 3: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/3.jpg)
Glioma: A Deadly Brain Cancer
Wikimedia commons
![Page 4: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/4.jpg)
Glioma Survival
http://www.neurooncology.ucla.edu/
5 years
10 years
Grade I Grade II Grade III Grade IV
![Page 5: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/5.jpg)
Neurophysiology
![Page 6: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/6.jpg)
Heterogeneity of Tumors
Mischel et al, 2004
![Page 7: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/7.jpg)
Hallmarks of Cancer
Hanahan et al. 2000
![Page 8: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/8.jpg)
Parallel Pathways of Tumorigenesis
Hanahan et al. 2000
Acquisition of hallmarks may vary mechanistically and chronologically:
![Page 9: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/9.jpg)
Can Genome-Wide Profiles Inform Cancer Treatment Planning?
Individual genome-wide profiles are sorted
Colon cancer patients
Have mutated K-ras gene
Profile A...give targeted
therapy A
Respond to targeted therapy A
Do not have mutated K-ras gene
A E A
A
AEE
E
B C C
B C
B
BC
B C
B C B
![Page 10: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/10.jpg)
Utility of Patient Stratification
Mischel et al, 2004
![Page 11: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/11.jpg)
Genome-Wide Profiling: Many Ways
![Page 12: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/12.jpg)
![Page 13: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/13.jpg)
miRNAs in Cancer
Caldas et al., 2005
RISC
![Page 14: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/14.jpg)
miRNAs are Dysregulated in Cancer
Chan et al., 2011
![Page 15: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/15.jpg)
Copy Number Variation
• Compare normal tissue vs. cancerous
• Amplifications
• Deletions
• Translocations
wikipedia.org
![Page 16: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/16.jpg)
Oncogene Amplification
http://www.cambridgebluegnome.com/copy_number_imbalance
![Page 17: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/17.jpg)
miRNAs are Trans-Acting Factors
![Page 18: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/18.jpg)
Gene Expression
http://www.news-medical.net/health/What-is-Gene-Expression.aspx
![Page 19: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/19.jpg)
Stratification of Patients
Mischel et al, 2004
![Page 20: Introduction to Glioblastoma Chris Plaisier Introduction to Systems Biology Course Institute for Systems Biology](https://reader036.vdocuments.site/reader036/viewer/2022062407/56649d9d5503460f94a862bb/html5/thumbnails/20.jpg)
Utility of miRNAs in Cancer
Chan et al., 2011