Download - How to conduct environmental sampling
ENVIRONMENTAL TESTING
• Order the appropriate sampling supplies from ABC Research Laboratories
• Clearly identify and label the swabs / sponges and sample submission form
• Learn aseptic sampling techniques before you begin to collect samples to prevent cross contamination
• Return samples via overnight shipping with frozen ice packs
Step by Step Guide:
ENVIRONMENTAL TESTING
ORDERING THE PROPER SUPPLIES
ENVIRONMENTAL TESTING
SPONGES OR SWABS
SWABS are best for small crevices or uneven surfaces, Example: drains and grates
SPONGES are best for smooth and flat surfaces, Example: floors, walls, doors
ENVIRONMENTAL TESTING
HOW TO IDENTIFY & LABEL SAMPLES
Before you begin to take the samples:
•Using a Sharpie or permanent marker, clearly label the sample bags / containers
•Use brief descriptions that clearly identify the sampling area or site
•Record the same descriptions on the sample submission form
ENVIRONMENTAL TESTING
ASEPTIC SAMPLING TECHNIQUES• Wash hands to mid-arms
with antibacterial soap before sampling
• Use clean laboratory gloves or the aseptic gloves provided
• Put the gloves on aseptically and avoid touching the finger portions of the gloves when putting them on
• GOAL: Avoid cross-contamination
ENVIRONMENTAL TESTING
• With bag still sealed, push the sponge towards the opening of the bag
• Open the bag without touching the sponge or inside of the bag
• With the sterile gloves on, remove the sponge from the bag careful to only touch the sides of the spongeGently wipe the sponge in a 10”x10” target area – wipe 10 times vertically and 10 times horizontally• Place the sponge back in the bag, expel excess air, and fold the top 3-4 times to seal
ENVIRONMENTAL TESTING
SWABS• Use clean laboratory
gloves or the aseptic gloves provided
• With the sterile gloves on, unscrew the cap of the swab container careful to only touch the lid and not the swabGently wipe the swab over a 2”x2” target
area – wipe vertically and horizontally
• Place the swab back into the tube and screw closed
ENVIRONMENTAL TESTING
PREPARING THE SHIPMENT• Securely seal the
sponge bag and swab container
• Place the swabs and sponges in ziploc bags for added protection
• Individual bags are best. If one leaks it will not cross contaminate the other samples
• GOAL: Avoid cross-contamination
ENVIRONMENTAL TESTING
PACK AND SHIP VIA OVERNIGHT
• Place completed sample submission form in a Ziploc bag to protect from spills
• Include plenty of frozen ice packs to maintain the proper temperature
• Package sponges, swabs, and frozen ice packs in an insulated cooler
• Ship via overnight delivery
ENVIRONMENTAL TESTING
We are here to help!To order environmental supplies, set up an ABC-R account, or just ask a question, we are always eager to provide answers. We
look forward to hearing from you!
352.372.0436866.233.5883
[email protected] www.abcr.com
