![Page 1: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/1.jpg)
Hepatoblastoma in the Mouse
David Malarkey, DVM, PhD National Institute of Environmental Health Sciences
NTP Technical Reports Peer Review Meeting
February 8-9, 2012
![Page 2: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/2.jpg)
• primitive, poorly differentiated variant of an hepatocellular neoplasm
• can arise from adenoma or carcinoma • relatively late onset • males >>> females • metastatic rate of ~ 25-50% • B6C3F1, D2B6F1, B6D2F1, CBAxC57BL/6J,
C3H, CF1, BDF1, Swiss Webster
Hepatoblastoma in the mouse
![Page 3: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/3.jpg)
Hepatoblastoma
![Page 4: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/4.jpg)
Hepatoblastoma
![Page 5: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/5.jpg)
Hepatoblastoma
![Page 6: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/6.jpg)
Hepatoblastoma in an adenoma
![Page 7: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/7.jpg)
Hepatoblastoma in a carcinoma
![Page 8: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/8.jpg)
Incidence of hepatoblastomas in B6C3F1 mice in NTP studies
incidence (mean; range) males 91 / 2954 (3.1%; 0-34%) females 9 / 3046 (0.3%; 0-2%) ________________________________________________ Grace Kissling, NTP
![Page 9: Hepatoblastoma in the Mouse...Hepatoblastoma in the Mouse David Malarkey, DVM, PhD National Institute of Environmental Health Sciences NTP Technical Reports Peer Review Meeting•](https://reader034.vdocuments.site/reader034/viewer/2022050716/5e4292c44349b20b75122251/html5/thumbnails/9.jpg)
Hepatoblastoma – Molecular studies
• Molecular studies aimed at understanding the pathogenesis of hepatoblastoma and the relationship between hepatoblastoma and hepatocellular carcinoma (HCC)
• Phenotypic characterization of hepatoblastomas in mice • Global gene expression analysis
– Comparing hepatoblastoma, HCC, and non-tumor liver in same animal
– Identification of differentially altered molecular pathways • Mutation analysis
– Common mutational spectrum – common origin of hepatoblastoma/HCC
– Differing mutational spectrum – supports differing origin/convergent pathogenesis