dna ant technology

8/2/2019 DNA ant Technology

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PRESENTATIONPresented by:

Shan-Salah-ud-Din 40

Rao-Abdul-Rahman 37

Ammad-Shahid 44

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Presented to:

Maam Fatima Ijaz

Presentation Topic:

Recombinant DNA TECH.

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What is recombination?

The exchangeofcorresponding DNAsegments between

adjacentchromosomes duringthe special type of

cell division thatresults in theproduction of new

genetic make up... 3


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What Is Recombinant DNA?Recombinant DNA is a form of DNA thatdoes not exist naturally, which is createdby combining DNA sequences that wouldnot normally occur together.In terms of genetic modification,recombinant DNA (rDNA) is introduced

through the addition of relevant DNA intoan existing organismal DNA.

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History of recombinant DNAtechnology

Recombinant DNA technology is one

of the recent advances inbiotechnology, which was developedby two scientists named Boyer and

Cohen in 1973.

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by natural and artificial processes, for practicalPurposes.

Intentionally modifying genomes of organisms,

Three Goals of Recombinant DNA Technology:

1. Eliminate undesirable phenotypic traits in humans, animals, plants

and microbes.2. Combine beneficial traits of two or more organisms to createvaluable new organisms.

3. Create organisms that synthesize products of humans need.

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DNA recombinant

technology

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DNA Recombinant Technology

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Basic principle of recombinantDNA technology

The DNA is inserted into another

DNA molecule called vector.

. The recombinant vector is thenintroduced into a host cell where itreplicates itself, the gene is thenproduced

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Objective:

Isolation of multiple copies of a desired gene andtransfer it to suitable organism to achieve: Large scale production of protein encoded by the gene.

Know the basics ofgene structure, function and regulation.

Be familiar with the basic methods of molecular genetics. Understand the meaning of DNA sequence and amino acid

polymorphisms.

The main purpose of rDNA technology is to make large

amounts of DNA (gene) &/or its Proteins. (Ex: Insulin).


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Steps involve in preparation of Recombinant DNA..Identification of desired Gene.

Isolation of Foreign DNA.

Formation of Chimeric

DNA.Insertion of Chimeric DNA in host Cell.

Selection of recombinant DNA.

Gene Cloning.

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Recombinant DNA Process Example:

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Applications ofRecombinant DNA

TechnologyLarge-scale production of humanproteins by genetically

engineered bacteria.

Such as : insulin, Growth

hormone, Interferon's.

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Vaccines productionFirstly, the gene in apathogenic virus that

stimulates protective immunityshould be identified.

That portion of DNA is thenisolated and incorporated intoan established harmless virus(e.g. vaccinia virus).

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This new recombinant virus isused as a vaccine.

These vaccines are muchsafer since they do not

expose the patients to theactual virus and do not riskto infection.

This method may be useful invaccines against malaria andmany viruses.

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Agricultural applicationsFrost resistant crops

Insecticide resistant crops

Herbicide resistant crops

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recombinant technology

It is always possible that anantibiotic-resistant plasmid could be

accidentally incorporated into adangerous pathogen with seriousmedical consequences.

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dna ant technology

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