dicer-like a rna helicase gene involved in gene silencing ucsf max bachourjessica chenchris...
TRANSCRIPT
Dicer-Like
A RNA Helicase Gene Involved in Gene Silencing
UCSF
Max Bachour Jessica Chen Chris McQuilkin
• RNA helicase is an enzyme that unwinds RNA and modifies its structure
• Involved in transcription, splicing, translation, and gene silencing
A RNA Helicase Gene Involved in Gene Silencing
RNA interference
• Dicer and Dicer-Like (DCL) enzymes are involved in RNA interference (RNAi)
• Nontranslated RNA fragments bind to mRNA and prevent translation into a protein
Evolutionary Origins
• A defense system for eukaryotes against double stranded viruses and transposons
What is Dicer’s role in RNAi?
• Activated by exogenous double-stranded (ds) RNA
• miRNA (micro RNA)-small, non-coding regions of double-stranded (ds) RNA 21-22 nucleotides
What is Dicer’s role in RNAi?
• siRNA (small interfering RNA)non-coding regions of dsRNA 20-25 nucleotides in length
How did the Dicer Knock-out Result in the Cone Shape?
•Knocking-out the dicer gene keeps RNAi from forming
•This keeps certain genes in the seed from being inhibited.
•The lack of inhibition caused the cells in the embryo to multiply irregularly