dialysis lab

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  • 1. Dialysis Lab and Nature of Science

2. Dialysis Lab Take out your lab notebook! If you have not finished your Cell Observations Lab conclusion, leave some space for your conclusion and skip to the next page Title: Dialysis Lab Purpose: To make observations of a dialysis apparatus in order to determine how dialysis tubing functions 3. Dialysis Lab Materials and MethodMaterials and Methods:- Corn Syrup- Dialysis Tubing- 500 mL beaker- 2 rubber bands- 10mL pipette- Water- Clamp and Ring Stand 4. Dialysis Lab - Materials and Method 5. Dialysis Lab - Materials and Method 6. Dialysis Lab - Materials and Method 7. Dialysis Lab - Results Write down any observations you see Then write down any questions you have about the apparatus. For Example: Why does ______________ happen? From one question you can come up with a hypothesis Maybe __________ happens because the pipette has a cottonin it. 8. Dialysis Lab - Results Then you can make an experiment around your hypothesis using an if then statement If the cotton ball in the pipette causes the liquid in the pipette change and we take out the cotton ball then, there will be no change in the amount of liquid in the pipette. Here are the materials available for your experiment After writing your hypothesis, have an instructor check it and you can write method for this experiment Be sure to include How you will be recording your data Only have 1 variable change only 1 thing A control 9. Gallery Walk When everyone is done with their method, we will walk around and annotate each groups method Write one thing that you like about the method and one thingthey can do to make it better Things to look for Does the method make sense to you? In other words, is it clear? Do they have a way to record their data qualitatively and quantitatively? Do they only have one variable? Make any adjustments necessary and start your new lab. 10. What was happening? Dialysis is a process that takes out waste and extra water from blood. It is an artificial replacement for kidneys. 11. The dialysis tube is madeof a permeablemembrane that works bysimple diffusion orpassive diffusion.Passive Diffusion:Molecules move from anarea of highconcentration to lowconcentration until it fillsthe space evenly(equilibrium) 12. Passive Diffusion: Molecules move from an area of high concentration to low concentration until it fills the space evenlyEx: Farts, Purfume, Food coloring in water 13. Cell MembraneThe cellmembrane isalso semi-permeable andwants tobalance with thewater from theenvironmentoutside. 14. Lipid BilayerLipid Bilayer: A thin membranemade of 2 layers of phospholipids. 15. Phospholipids: lipids with polar head (hydrophilic) and non-polartail (hydrophobic) that make up the cell membrane. 16. But Wait! If the fatty acid tails on the phospholipid are non-polar, how do the water molecules go through?Aquaporins! Proteins in the cell membrane that have atunnel for water to pass through. 17. Aquaporins! Proteins in the cell membrane that have a tunnelfor water to pass through using passive transport 18. Types of Transport ProteinsPassiveTransport:Movingmolecules in andout of amembranewithout usingenergy. Can onlymove from highto lowconcentration 19. Active Transport:Movingmolecules in andout of amembrane usingenergy.