diagnostic service portfolio

DIAGNOSTIC SERVICE PORTFOLIO

CONSULTING

AniCon Labor GmbH | Muehlenstr. 13 | 49685 Hoeltinghausen | Germany Phone +49 44 73 94 38 30 | [email protected] | www.anicon.eu

POULTRY

Dr. Swaantje Rönchen

Phone: +49 44 73 / 94 38 - 52

[email protected]

Dr. Diana Petzoldt

Phone: +49 4473 / 94 38 - 786

[email protected]

All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 01.06.2021

Diagnostic Service Portfolio Poultry of 9

G.2.01, 2021.01.06 | Rev007

Item-No Pathology 19230 Sampling "basic" 19300 Sampling "extended" 19240 Pathological-anatomical reporting 12690 Parasitologic investigation (mucosal scraping; microscopic examination) 12690 Parasitologic investigation (flotation/sedimentation; microscopic examination) 10318 Parasitologic investigation (feces: coccidia oocyst counting acc. to McMaster) 10358 Patho-histological examination (per tissue sample)

Item-No Material for sampling 27960 AniCard with 10 spots (FTA®-card technology) (per card) 28560 Reaction tube 1.5 ml (per 500 tubes) 28520 Tube 13 ml and plug (per 500 tubes) 28580 Salmonella-sampling swab (with neutralizer solution; per 50 swabs) 28590 Boot/Sock swab (per 100 swabs) 23250 MALDI-TOF MS target (single use) 28550 Sampling beaker sterile (per 5 beaker) 29650 Sampling beaker with formalin for pathohistology (per beaker) 28570 Swab for anaerobic bacteria (per 50 swabs) 15160 Fine swab (aluminium) with transport medium (per 100 swabs) 12810 Fine swab (aluminium) without transport medium (per 100 swabs) 19910 Swab (wooden) without transport medium (per 100 swabs) 26780 Swab with preservative agent (Mycoplasma or virus culture) (per 50 swabs) 27460 Swab with preservative agent (Brachyspira culture) (per 50 swabs) 19930 Paper strips (ELISA) (per 100 strips)



G.2.01, 2021.01.06 | Rev007

Item-No ELISA 20520 Avian Encephalomyelitis Virus (AEV) 11310 Avian Metapneumovirus A & B (aMPV / ART / TRT / SHS) 11680 Bordetella avium (turkey) 20530 Chicken Anemia Virus (CAV) 19830 Chicken Astrovirus (CAstV; cave: group B only) 10231 Egg Drop Syndrome-76 Virus (EDS-76 / DuAdV) 19110 Fowl Adenovirus (FAdV) 12860 Hemorrhagic Enteritis Virus of Turkeys (HEV) 12930 Infectious Bursitis Virus (IBDV / Gumboro)# 20490 Infectious Bursitis Virus (IBD plus; in case of vaccination with Vaxxitek® IBD) 13280 Infectious Bronchitis Virus (IBV) 17390 Infectious Laryngotracheitis Virus (ILTV) 12830 Influenza Virus Type A (multispecies / blocking) 14470 Influenza Virus - H5 specific (multispecies / blocking) 14480 Influenza Virus - H7 specific (multispecies / blocking) 10730 Mycoplasma gallisepticum (Mg) 10740 Mycoplasma synoviae (Ms) 12660 Mycoplasma meleagridis (Mm) 16470 Newcastle Disease Virus (aPMV-1) 10840 Ornithobacterium rhinotracheale (ORT) 16580 Pasteurella multocida (chicken, turkey) 15800 Reovirus (ARV) 10750 Riemerella anatipestifer (turkey & duck only, please indicate species) 15680 Salmonella Group B & D (incl. SE & ST; chicken)

# vaccination date prediction is provided upon request (at no extra charge)

Item-No HI – Hemagglutination Inhibition Testing 14520 Egg Drop Syndrome-76 Virus (EDS-76 / DuAdV) 17380 Influenza H-Type (H1, H5, H6, H7, H9, further upon request) 15950 Avian Paramyxovirus-1 (Newcastle Disease Virus, NDV, aPMV-1) 15970 Avian Paramyxovirus-3 (aPMV-3)

Item-No SSA – Serum Slide Agglutination 10780 Mycoplasma gallisepticum (Mg) 10790 Mycoplasma meleagridis (Mm) 10860 Mycoplasma synoviae (Ms) 10800 Salmonella Gallinarum Pullorum (SGP)



G.2.01, 2021.01.06 | Rev007

Item-No Microbiology 10580 Bacteriological examination (per swab / animal) 13920 Bacteriological examination package$ 1-5 chicks / poults 13790 Bacteriological examination package$ 6-14 chicks / poults 12750 Biochemical differentiation - API 18400 MALDI-TOF MS (species determination by mass spectrometry bacteria) 24300 MALDI-TOF MS (species determination by mass spectrometry mould) 10590 Resistance testing (ADT) 10064 Resistance testing (MIC) 14030 Archival storage (bacterial & viral strains) 23860 Cultural enrichment – Brachyspira spp. 23000 Cultural enrichment - Campylobacter spp. 16350 Cultural enrichment - Clostridium spp. 19690 Cultural enrichment - Mycoplasma spp. 18480 Selective cultivation of fungi (without species differentiation) 12190 Cultivation Salmonella spp. (acc. ISO 6579) 12720 Cultivation Salmonella spp. 5-fold passaging (in-house method) 23010 Salmonella spp. - serovar-typing according to Kauffmann-White scheme

18270 Salmonella Enteritidis Vaccine/Field strain-differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics)

18270 Salmonella Typhimurium Vaccine/Field strain-differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics)

13040 Serotyping (slide agglutination) E. coli 13020 Serotyping (agarose gel precipitation) ORT 10096 Serotyping (acc. to Heddleston) Pasteurella multocida 13030 Serotyping (slide agglutination) Riemerella anatipestifer

$ includes cultural examination for Salmonella spp. and basic bacteriological examination (price excl. further examinations, such as resistance testing etc.)

1,2,3,4: For explanation and comments see page 9



G.2.01, 2021.01.06 | Rev007

Item-No Viral PCR1 & Sequencing 17720 Avian Encephalomyelitis Virus (AEV) 12300 Avian Influenzavirus Type A (“Matrix-PCR”, AIV) 16690 Influenza H3 13780 Influenza H5 & N1 combination 14650 AIV, H5N1 + H7 combination 16690 Influenza H6 13880 Influenza H7 16690 Influenza H9 10149 Influenza-N-type determination N1 to N9 (price per each additional N-antigen) 16690 Influenza-H-type determination H1 to H16 (price per each additional H-antigen) 24740 Influenza H5/H7 sequencing for HPAI/LPAI differentiation 19570 Avian Hepevirus (aHEV/ Avian Hepatitis E virus/ BLSD virus) 12650 Avian Metapneumovirus A & B (serotype-specific, aMPV-A & -B / ART / TRT / SHS) 14670 Avian Metapneumovirus C & D (serotype-specific, aMPV -C & -D) 14660 Avian Metapneumovirus A to D (serotype-specific, combination) 20770 Avian Nephritis Virus 1 & 2 (ANV-1 & -2) 20720 Avian Nephritis Virus 3 (ANV-3) 17740 Avian Ortho-Reovirus (ARV)2 24700 Avian Ortho-Reovirus sequencing & phylogenetic analysis (C- and/or NS-gene) 17740 Avian Ortho-Reovirus S1133 DIVA3-PCR 22310 Avian Polyomavirus (GHPV / Hemorrhagic Nephritis and Enteritis Virus of Geese / HNEG) 17800 Avian Rotavirus A & D (serotype-specific, combination) 19850 Pheasant Rotavirus 10356 Pigeon Rotavirus 20290 Chicken Anemia Virus (CAV) 20510 Chicken Astrovirus (CAstV, group A & B) 10124 Chicken Astrovirus sequencing for differentiation of group A & B 28500 Chicken Proventricular Necrosis Virus (Transmissible Viral Proventriculitis / CPNV / TVP) 19770 Duck / Goose Reovirus (DRV / GRV) 17820 Duck / Goose Circovirus (DuCV / GoCV; species-specific) 16790 Duck Egg Drop Flavivirus (BYDV / Baiyangdian) 16510 Duck Enteritis Virus (Duck plague, DEV) 16500 Duck Hepatitis A Virus Type 1 (DHAV-1 / DHV-1)




G.2.01, 2021.01.06 | Rev007

Item-No Viral PCR1 & Sequencing (continuing) 20010 Muscovy Duck Parvovirus & Goose Parvovirus (MDPV, GPV) Screening-PCR 22290 MDPV/GPV Typing-PCR for differentiation of Goose Parvovirus/Muscovy Duck Parvovirus 10182 Duck & Goose Parvovirus DIVA³-PCR (Hoekstra) 17770 Egg Drop Syndrome-76-Virus (EDS-76 / DuAdV) 17780 Fowl Adenovirus (FAdV)2 24720 Fowl Adenovirus sequencing for species and serotype determination 23900 Fowlpox Virus (FPV) 13960 Infectious Bronchitis Virus (IBV) / Avian Coronavirus (ACoV) screening (incl. IBV & TuCV) 18700 IBV-variant-specific determination (package of all available variants)4 24680 IBV sequencing & phylogenetic analysis (S1) 16250 Infectious Laryngotracheitis Virus (ILTV) 15760 IBDV screening incl. patho-typing (Gumboro, differentiation between vvIBDV and non-vvIBDV) 24690 IBDV sequencing & phylogenetic analysis (VP1 & 2) 27500 IBDV conceptual translation (12 amino acids) 27510 IBDV conceptual translation (28 amino acids) 28640 Innovax®-ND-IBD DIVA3-PCR 19650 Herpes Virus of Turkeys (GaHV-3, HVT) 19180 Marek's Disease Virus (MDV, GaHV-1) 23820 Marek & Rispens DIVA3-PCR (separate detection of non-Rispens and Rispens strain) 10119 Marek's Disease Virus sequencing 20500 Newcastle Disease Virus (Avian Paramyxovirus-1, aPMV-1) 24710 Newcastle Disease Virus / aPMV-1 patho-typing-PCR (lentogenic vs. meso- & velogenic)

24710 Newcastle Disease Virus / aPMV-1 sequencing & patho-typing (assortment to lento-, meso- or velogenic)

19670 Reticuloendotheliosis Virus (REV) 20000 Turkey Astrovirus 1 / 2 (serotype-specific, TAstV-1/-2) 19630 Turkey Coronavirus (TuCV) 18730 Turkey Hemorrhagic Enteritis (TuHEV) 16970 Turkey Viral Hepatitis (TVH) 29390 Vaxxitek® HVT+IBD DIVA3-PCR 10101 Vectormune® ND DIVA3-PCR




G.2.01, 2021.01.06 | Rev007

Item-No PCR1 and Sequencing - Mycoplasma 14640 Mollicutes spp. 12450 Mycoplasma gallisepticum (Mg) 14710 Mg DIVA3-PCR vaccine strain 6/85-like and F-like-strain 23740 Mg DIVA3-PCR vaccine strain ts-11 19190 Mycoplasma iowae (Mi) 19130 Mycoplasma meleagridis (Mm) 13050 Mycoplasma synoviae (Ms) 19290 Ms-H DIVA3-PCR (incl. Ms field strain detection) 22430 Ms-1 DIVA3-PCR 12440 Mg & Ms (species-specific, combination)

Item-No PCR1 and Sequencing - Chlamydia 19840 Chlamydia spp. screening, C. abortus & C. psittaci (species-specific, combination) 14020 Chlamydia spp. screening 19680 Chlamydia abortus 19780 Chlamydia avium (doves, psittacidae) 19790 Chlamydia gallinacea 10088 Chlamydia gallinacea

Item-No PCR1 and Sequencing - Diverse 14540 Avibacterium paragallinarum (Infectious Coryza) 18870 Bordetella avium 21530 Brachyspira spp.-screening 10311 Brachyspira hyodysenteriae, B. pilosicoli (species-specific, combination) 10333 Brachyspira intermedia 27520 Cryptosporidium parvum

14610 Thermophilic Campylobacter spp. (incl. C. coli, C. jejuni, C. lari, C. upsaliensis; excl. C. hepaticus)

10086 Campylobacter coli, C. jejuni, C. lari (species-specific, combination) 10087 Campylobacter hepaticus 14550 Clostridium perfringens major & minor toxin genes# 14630 Erysipelothrix rhusiopathiae (Erysipelas)

# The following toxin genes are analysed: major toxin genes alpha, beta, epsilon & iota and minor toxin genes beta2, enterotoxin & netB




G.2.01, 2021.01.06 | Rev007

Item-No PCR1 and Sequencing – Diverse (continuing) 10355 Escherichia coli 14700 E. coli 8 x APEC (virulence genes factors; incl. F11-antigen) 10282 E. coli 15-fach APEC (virulence genes factors; incl. F11-antigen) 15680 Histomonas meleagridis 10228 Lawsonia intracellularis 13800 Ornithobacterium rhinotracheale (ORT) 17590 Pasteurella multocida 24370 Pasteurella multocida subsp. septica 14680 Pasteurella multocida capsule typing 13430 Riemerella anatipestifer 22460 Salmonella spp.

24110 SGP DIVA3-PCR (serovar-specific differentiation of Salmonella Gallinarum and Pullorum and vaccine strain 9R specific)

19370 SE DIVA3-PCR (Salmonella Enteritidis serovar-specific & vaccine strains IDT/CEVA and LAH/ELANCO specific) combined with ST (Salmonella Typhimurium serovar-specific)

22540 Staphylococcus aureus # The following toxin genes are analysed: major toxin genes alpha, beta, epsilon & iota and minor toxin genes beta2, enterotoxin & netB.

Item-No Respiratory Pathogens - Scaffold Pricing of Packages* 20810 PCR-package of 2 respiratory pathogens (bact. / viral)* 20820 PCR-package of 3 respiratory pathogens (bact. / viral)* 20830 PCR-package of 4 respiratory pathogens (bact. / viral)* 20840 PCR-package of 5 respiratory pathogens (bact. / viral)* 20850 PCR-package of 6 respiratory pathogens (bact. / viral)* 22370 PCR-package of 7 respiratory pathogens (bact. / viral)* 22380 PCR-package of 8 respiratory pathogens (bact. / viral)* 22390 PCR-package of 9 respiratory pathogens (bact. / viral)*

* does not apply for DIVA3-PCRs, typing-PCRs or other PCR-packages and sequencing




G.2.01, 2021.01.06 | Rev007

Item-No Cultural Virus Isolation 17700 Virus cultivation in cell culture / in embryonated egg (excl. PCR for confirmation) 14030 Archival storage (bacterial & viral strains) 19980 Combination FAdV & ARV: culture & PCR

Explanation and comments:

1 Samples of up to five individuals can be pooled. It is crucial to indicate when sending several swabs whether these are already pooled or need to be pooled. Please find respective submission forms on www.anicon.eu → downloads. 2 Direct diagnostics of Fowl Adenovirus (FAdV) and/ or Avian Ortho-Reovirus (ARV) from raw samples by PCR methods may lead to false negative results; we run at least three passages of virus cultivation before confirmation by PCR methods in harvested cultural material, if not explicitly ordered for PCR only. Please see also article „combination FAdV & ARV: culture and PCR“ in chapter virology. 3 DIVA - Differentiating Infected from Vaccinated Animals - method for differentiation of field strains from named vaccine strains. 4 793B (4/91, CR88); QX (D388); D1466; Massachusetts; D274; Italy 02; Arkansas; Variant 2 (Israel 02/IS1494); Q1; IB80; D181

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