diagnostic service portfolio - anicon€¦ · diagnostic service portfolio poultry page 3 of 10...

DIAGNOSTIC SERVICE PORTFOLIO

CONSULTING

AniCon Labor GmbH | Muehlenstr. 13 | 49685 Hoeltinghausen | Germany Phone +49 44 73 94 38 30 | [email protected] | www.anicon.eu

POULTRY

Dr. Swaantje Rönchen

Phone: +49 44 73 / 94 38 - 52

[email protected]

Dr. Diana Petzoldt

Phone: +49 4473 / 94 38 - 786

[email protected]

All prices are exclusive value-added tax. – Our general terms and conditions apply, available on www.anicon.eu. Hoeltinghausen, 10.03.2020

Diagnostic Service Portfolio Poultry of 10

G.1.01, 2020.03.10 | Rev006

Pathology

Selective sampling for bacteriology

Extended sampling efforts for PCR and / or cultural virology

Pathological-anatomical reporting

Parasitologic investigation (mucosal scraping; microscopic examination)

Parasitologic investigation (flotation/sedimentation; microscopic examination)

Patho-histological examination (per tissue sample)

Material for sampling

AniCard with 10 spots (FTA®-card technology) (per card)

Reaction tube 1,5 ml (per 500 tubes)

Tube 13 ml and plug (per 500 tubes)

Salmonella-sampling swab (with neutralizer solution; per 50 swabs)

Boot/Sock swab (per 100 swabs)

Sampling beaker sterile (per 5 beaker)

Swab for anaerobic bacteria (per 50 swabs)

Fine swab (aluminium) with transport medium (bacteriology) (per 100 swabs)

Fine swab (aluminium) without transport medium (bacteriology/PCR) (per 100 swabs)

Swab (wooden) without transport medium (bacteriology) (per 100 swabs)

Swab with preservative agent (Mycoplasma or virus culture) (per 50 swabs)

Swab with preservative agent (Brachyspira) (per 50 swabs)

Paperstrips (ELISA) (per 100 strips)

http://www.anicon.eu/



G.1.01, 2020.03.10 | Rev006

ELISA

Fowl Adenovirus (FAdV)

Avian Encephalomyelitis Virus (AEV)

Bordetella avium (turkey)

aMPV / ART / TRT / SHS (subtypes A & B)

Chicken Anemia Virus (CAV)

Chicken Astrovirus (CAstV; cave: group B only)

Hemorrhagic Enteritis Virus of Turkeys (HEV)

Gumboro (IBDV)#

Gumboro (IBD plus; in case of vaccination with Vaxxitek® IBD)

Infectious Bronchitis Virus (IBV)

Infectious Laryngotracheitis Virus (ILTV)

Influenza Virus Type A (multispecies / blocking)

Influenza Virus - H5 specific (multispecies / blocking)

Influenza Virus - H7 specific (multispecies / blocking)

Newcastle Disease Virus (aPMV-1)

Mycoplasma gallisepticum (Mg)

Mycoplasma synoviae (Ms)

Mycoplasma meleagridis (Mm)

Ornithobacterium rhinotracheale (ORT)

Pasteurella multocida (chicken, turkey)

Riemerella anatipestifer (turkey & duck only, please indicate species)

Reovirus (ARV)

Salmonella Group B & D (incl. SE & ST; chicken)

# vaccination date prediction is provided upon request (at no extra charge)




G.1.01, 2020.03.10 | Rev006

HI – Hemagglutination Inhibition Testing

Egg Drop Syndrome-76 Virus (EDS-76 / DuAdV)

Influenza H-Type (H1, H5, H6, H7, H9, further upon request)

Newcastle Disease Virus (aPMV-1)

aPMV-3

SSA – Serum Slide Agglutination




Salmonella Gallinarum Pullorum (SGP)




G.1.01, 2020.03.10 | Rev006

Microbiology

Bacteriological examination (per swab / animal)

Bacteriological examination package$ 1-5 chicks / poults

Bacteriological examination package$ 6-14 chicks / poults

Biochemical differentiation - API

MALDI-TOF MS (species determination by mass spectrometry)

Resistance testing (ADT)

Resistance testing (MIC)

Archival storage (bacterial & viral strains)

Cultural enrichment – Brachyspira spp.

Cultural enrichment - Clostridium spp.

Cultural enrichment - Mycoplasma spp.

Selective cultivation of fungi (without species differentiation)

Cultivation Salmonella spp. (acc. ISO 6579)

Cultivation Salmonella spp. 5-fold passaging (in-house method)

Salmonella spp. - serovar-typing according to Kauffmann-White scheme

Salmonella Enteritidis Vaccine/Field strain-Differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics) Salmonella Typhimurium Vaccine/Field strain-Differentiation (Erythromycin/Rifampicin-Sensitivity testing (LAH) and IDT Salmonella Diagnostics)

E. coli serotyping (slide agglutination)

ORT serotyping (agarose gel precipitation)

Pasteurella multocida serotyping (acc. to Heddleston)

Riemerella serotyping (slide agglutination) $ includes cultural examination for Salmonella spp. and basic bacteriological examination (price excl. further examinations, such as resistance testing etc.)




G.1.01, 2020.03.10 | Rev006

Viral PCR1 & Sequencing

Avian Encephalomyelitis Virus (AEV)

Avian Influenza Type A (“Matrix-PCR”, IVA)

Influenza H3

Influenza H5 & N1 combination

Influenza H6

Influenza H7

Influenza H9

Influenza-N-type determination N1 to N9 (price per each additional N-antigen)

Influenza-H-type determination H1 to H16 (price per each additional H-antigen)

Influenza H5/H7 sequencing for HPAI/LPAI differentiation

Avian Hepevirus (aHEV/ Avian Hepatitis E virus/ BLSD virus)

Avian Metapneumovirus A & B (serotype-specific, aMPV-A & -B / ART / TRT / SHS)

Avian Metapneumovirus C & D (serotype-specific, aMPV -C & -D)

Avian Metapneumovirus A to D (serotype-specific, combination)

Avian Nephritis Virus 1 & 2 (ANV-1 & -2)

Avian Nephritis Virus 3 (ANV-3)

Avian Ortho-Reovirus (ARV)2

Avian Ortho-Reovirus sequencing & phylogenetic analysis (C- and/or NS-gene)

Avian Ortho-Reovirus S1133 DIVA3-PCR

Duck / Goose Reovirus (DRV / GRV)

Avian Polyomavirus (GHPV / Hemorrhagic Nephritis and Enteritis Virus of Geese / HNEG)

Avian Rotavirus A & D (serotype-specific, combination)

Pheasant Rotavirus

Pigeon Rotavirus

Chicken Anemia Virus (CAV)

Chicken Astrovirus (CAstV, group A & B)

Chicken Astrovirus sequencing for differentiation of group A & B

Chicken Proventricular Necrosis Virus (Transmissible Viral Proventriculitis / CPNV / TVP)

Duck / Goose Circovirus (DuCV / GoCV; species-specific)

Duck Egg Drop Flavivirus (BYDV / Baiyangdian)

Duck Enteritis Virus (Duck plague, DEV)

Duck Hepatitis A Virus Type 1 (DHAV-1 / DHV-1)

Muscovy Duck Parvovirus & Goose Parvovirus (MDPV, GPV) Screening-PCR

MDPV/GPV Typing-PCR for differentiation of Goose Parvovirus/Muscovy Duck Parvovirus

Duck & Goose Parvovirus DIVA³-PCR (Hoekstra)

1,2,3,4: For explanation and comments see page 10




G.1.01, 2020.03.10 | Rev006

Viral PCR1 & Sequencing (continuing)

Egg Drop Syndrome-76-Virus (EDS-76 / DuAdV)

Fowl Adenovirus (FAdV)2

Fowl Adenovirus sequencing for species and serotype determination

Fowlpox Virus (FPV)

Infectious Bronchitis Virus / Avian Coronavirus screening (incl. IBV & TuCV)

IBV-variant-specific determination (package of all available variants)4

IBV sequencing & phylogenetic analysis (S1)

Infectious Laryngotracheitis Virus (ILTV)

IBDV screening incl. patho-typing (Gumboro, differentiation between vvIBDV and non-vIBDV)

IBDV sequencing & phylogenetic analysis (VP1 & 2)

IBDV conceptual translation (12 amino acids)

IBDV conceptual translation (28 amino acids)

Innovax®-ND-IBD DIVA3-PCR

Herpes Virus of Turkeys (GaHV-3, HVT)

Marek's Disease Virus (MDV, GaHV-1)

Marek & Rispens DIVA3-PCR (separate detection of field and Rispens strain)

Marek's Disease Virus sequencing

Newcastle Disease Virus (Avian Paramyxovirus-1, aPMV-1)

Newcastle Disease Virus / aPMV-1 patho-typing-PCR (lentogenic vs. meso- & velogenic)

Newcastle Disease Virus / aPMV-1 sequencing & patho-typing (assortment to lento-, meso- or velogenic)

Reticuloendotheliosis Virus (REV)

Turkey Astrovirus 1 / 2 (serotype-specific, TAstV-1/-2)

Turkey Coronavirus (TuCV)

Turkey Hemorrhagic Enteritis (TuHEV)

Turkey Viral Hepatitis (TVH)

TVH sequencing & phylogenetic analysis

Vaxxitek® HVT+IBD DIVA3-PCR

Vectormune® ND DIVA3-PCR





G.1.01, 2020.03.10 | Rev006

PCR1 and Sequencing - Diverse

Mycoplasma

Mollicutes spp.


Mg DIVA3-PCR vaccine strain 6/85-like and F-like-strain

Mg DIVA3-PCR vaccine strain ts-11


Ms-H DIVA3-PCR (incl. Ms field strain detection)

Ms-1 DIVA3-PCR

Mg & Ms (species-specific, combination)


Mycoplasma iowae (Mi)

Chlamydia

Chlamydia spp. screening, C. abortus & C. psittaci (species-specific, combination)

Chlamydia spp. screening

Chlamydia abortus

Chlamydia avium (doves, psittacidae)

Chlamydia gallinacea

Chlamydia psittaci





G.1.01, 2020.03.10 | Rev006

PCR1 and Sequencing – Diverse (continued)

Others

Fungi species determination (e.g. Aspergillus; by sequencing)

Avibacterium paragallinarum (Infectious Coryza)

Bordetella avium

Brachyspira spp.-screening

Thermophilic Campylobacter spp. (incl. C. coli, C. jejuni, C. lari, C. upsaliensis; excl. C. hepaticus)

Campylobacter coli, C. jejuni, C. lari (species-specific, combination)

Campylobacter hepaticus

Clostridium perfringens major & minor toxin genes#

Erysipelothrix rhusiopathiae (Erysipelas)

Escherichia coli

Escherichia coli 8 x APEC (virulence factors; incl. F11-antigen)

Histomonas meleagridis

Ornithobacterium rhinotracheale (ORT)

Pasteurella multocida

Pasteurella multocida subsp. septica

Pasteurella multocida capsule typing

Riemerella anatipestifer

Salmonella spp.

SGP DIVA4-PCR (serovar-specific differentiation of Salmonella Gallinarum and Pullorum and vaccine strain 9R specific) SE DIVA4-PCR (Salmonella Enteritidis serovar-specific & vaccine strains IDT and LAH/ELANCO specific) combined with ST (Salmonella Typhimurium serovar-specific)

# The following toxin genes are analysed: major toxin genes alpha, beta, epsilon & iota and minor toxin genes beta2, enterotoxin & netB.

Respiratory Pathogens - Scaffold Pricing of Packages*

PCR-package of 2 respiratory pathogens (bact. / viral)*








* does not apply for DIVA4-PCRs, typing-PCRs or other PCR-packages and sequencing





G.1.01, 2020.03.10 | Rev006

Cultural Virus Isolation

Virus cultivation in cell culture / in embryonated egg (excl. PCR for confirmation)

Archival storage (bacterial & viral strains)

Combination FAdV & ARV: culture & PCR

Explanation and comments:

1 Samples of up to five individuals can be pooled. It is crucial to indicate if sending several swabs whether these are already

pooled or need to be pooled. Please find respective submission forms on www.anicon.eu → downloads. 2 Direct diagnostics of Fowl Adenovirus (FAdV) and/ or Avian Ortho-Reovirus (ARV) from raw samples by PCR methods may

lead to false negative results; we run at least three passages of virus cultivation before confirmation by PCR methods in

harvested cultural material, if not explicitly ordered for PCR only. Please see also article „combination FAdV & ARV: culture

and PCR“ in chapter virology.

3 DIVA - Differentiating Infected from Vaccinated Animals - method for differentiation of field strains from named vaccine

strains.

4 793B (CR88, 1/96, 4/91), QX (D388), D1466, Massachusetts, D274, Italy 02, Arkansas, Variant 2 (Israel 02/IS1494), Q1,

IB80, D181


diagnostic service portfolio - anicon€¦ · diagnostic service portfolio poultry page 3 of 10...

Documents