demystifying fusarium diseases of tomato workgroup... · fusarium oxysporum f. sp. lycopersici igs...
TRANSCRIPT
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Demystifying Fusarium Diseases of Tomato
R. M. Davis Dept. of Plant Pathology
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http://fr.wikipedia.org/wiki/Fusarium
Fusarium oxysporum
Fusarium solani
Macroconidia Microconidia Chlamydospores
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Fusarium Crown and Stem Rot Fusarium striatum Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Fusarium Wilt Fusarium oxysporum f. sp. lycopersici Race 1 Race 2 Race 3
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Fusarium Crown and Stem Rot Fusarium striatum Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Fusarium Wilt Fusarium oxysporum f. sp. lycopersici Race 1 Race 2 Race 3
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Hosts:Tomato, potato, eggplant
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Gene’s slides foot rot
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Fusarium Crown and Stem Rot Fusarium striatum Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Fusarium Wilt Fusarium oxysporum f. sp. lycopersici Race 1 Race 2 Race 3
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Plant Disease 98:292-298 Moine et al.
Fusarium Crown and Stem Rot Fusarium striatum
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F. solani f. sp. eumartii CA
Fusarium solani f. sp. eumartii and F. striatum from tomato
EF and ITS sequences
F. solani f. sp. eumartii Japan
F. striatum Canada
F. striatum CA
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Fusarium Crown and Stem Rot Fusarium striatum Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Fusarium Wilt Fusarium oxysporum f. sp. lycopersici Race 1 Race 2 Race 3
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Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Hosts: some legumes, cucurbits, other solanaceous plants, and more
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Knight’s Landing
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F. oxysporum f. sp. radicis-lycopersici FL
Fusarium oxysporum f. sp. radicis-lycopersici
IGS and EF sequences
F. oxysporum f. sp. radicis-lycopersici FL, CA, Japan
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Fusarium Foot Rot Fusarium solani f. sp. eumartii Fusarium Crown and Stem Rot Fusarium striatum Fusarium Crown and Root Rot Fusarium oxysporum f. sp. radicis-lycopersici Fusarium Wilt Fusarium oxysporum f. sp. lycopersici Race 1 Race 2 Race 3
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Fusarium Wilt Fusarium Crown and Root Rot F. o. lycopersici F. o. radicis-lycopersici
1. Wilt 1. Crown and root rot
2. Moves rapidly in vascular tissue 2. No movement in vascular tissue
3. Optimum temperature: 27 C (80 F) 3. Optimum temperature: 18 C (64 F)
4. Limited host range: tomato 4. Wide host range: beans, beets,
cucumber, barley, onion, asparagus
5. Genetics 5. Genetics
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Fusarium Wilt
Fusarium oxysporum f. sp. lycopersici
3 races, 1, 2, and 3
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Fusarium Verticillium
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Race 2 CA
Race 3 CA, FL, MX
Race 2 FL, AR
Race 1 CA, Japan
Race 2 CA, FL
Race 3 FL, CA (14X97)
Cai et al Phytopathol 93:1014-1022
VCG 0030
VCG 0033
VCG 0031
VCG 0032
VCG 0035
Fusarium oxysporum f. sp. lycopersici
IGS sequences and VCGs
Race 2 OH
Race 1 FL, Italy
Race does not correlate with VCG or genetic similarity
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Race of Tomato plant F. o. lycopersici i i2 i3 I i2 i3 I I2 i3 I I2 I3 1 AVR1, AVR2, AVR3 S R R R 2 --- , AVR2, AVR3 S S R R 3 --- , avr2, AVR3 S S S R
Race 2 from Race 1 by transposon insertion into AVR1
Race 3 from Race 2 by point mutation in AVR2
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Propagules of FOV in Stem Tissue
0
500
1000
1500
2000
0 10 1̂ 10 2̂ 10 3̂ 10 4̂ 10 5̂ 10 6̂
Inoculum density
No.
of c
olon
ies/
g
DP744Ph72Ph800
0 101 102 103 104 105 106
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Low P Low K
= foliage necrosis and dieback
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Movement • Seed • Any way soil and infected crop debris is moved
Management • Containment • Clean seed • Soil fumigation • Rotation • Resistance