CytologyBY: MOSTAFA SAFIAN

نسألكم الدعاء

بسم الله الرحمن الرحيم

Mitochondria

-stain :

iron haematoxlin

-structure :black granules or filaments forming the basal striations in the striated duct -occurrence :in salivary glands- liver- kidney- muscle

Mostafa Safian

Mitochondria

Mostafa Safian

Golgi apparatus

-stain : silver impregnation

-structure :browen or black fibillar,

round or oval structures

-occurrence :in the apical cytoplasm

of the epithelium lining the epididymis

Mostafa Safian

Myofibrils

-stain : 1-Haematoxylin&Eosin

2-Silver impregnation

-structure :-in skeletal muscle fiber:

-in longitudinal section, the myofibrilsform light and dark bands

-in cross section, the myofibrils appearas fine red points in a polyhedral shaped muscle fiber

-occurrence :skeletal muscle fiber

Mostafa Safian

Myofibrils

Mostafa Safian

Myofibrils

Mostafa Safian

Myofibrils

Mostafa Safian

Proteins(zymogen granules)

-stain :-Haematoxylin&Eosin

-structure :Acidophilic(red) substance

filling the apical cytoplasm of the pancreatic acinar cells

representing the zymogen granules

-occurrence :In pancreatic acinar cells

Mostafa Safian

Melanin pigment

-structure :Melanin granules

( black to brown) scatter in the iris

or ciliary processes of the eye

and in the basal layer of skin

-occurrence :Ciliary process of eye or skin

Mostafa Safian

Melanin pigment

Mostafa Safian

Goblet cell(carbohydrate)

-stain :

Periodic acid Schiff(PAS)

-structure :goblet cell :

exocrine unicellular gland consist of two parts

1-apical expended portion appear emptydue to dissolved carbohydrates

2 -narrow slender distal portion contain compressed nucleus

-occurrence :Small intestine

Mostafa Safian

Goblet cell(carbohydrate)

Mostafa Safian

Goblet cell(carbohydrate)

Stain:(PAS)

Mostafa Safian

Goblet cell(carbohydrate)

Stain:(PAS)

Mostafa Safian

Goblet cell(carbohydrate)

Stain:(PAS)

Mostafa Safian

Goblet cell(carbohydrate)

1-Eosin & hematoxylin

2-Stain:(PAS)

Mostafa Safian

Lipid

-stain :

1-Hx & Eosin2-Osmic acid or Suddan black

-structure :1- By Hx & Eosin the fat cells appear

as clear empty space surrounded by thin film of red cytoplasm

containing flat basophilic nucleus

2 -By osmic acidor suddan black the flat cells appear as large sherical or round homogenous black spots

-occurrence :Perineural fat

Mostafa Safian

Lipid

Mostafa Safian

Lipid

Stain: Sudan black

Mostafa Safian

Lipid

Stain: Sudan black

Mostafa Safian

Lipid

Stain: Sudan black

Mostafa Safian

Simple squamousepithelium

-structure :Appear as single layer of flat cells.

their cytoplasm contains flat nucleus

located in the wide central part

-occurrence :Parietal layer of

bowman's capsule

Mostafa Safian

Simple squamousepithelium

Mostafa Safian

Simple squamousepithelium

Mostafa Safian

Simple squamousepithelium

Mostafa Safian

Simple cuboidal

-structure :Single layer of cubic shaped cells

(height and width nearly equal .)the cells appear as squares, each

cell contains round central nucleus

-occurrence :Thyroid follicle

Mostafa Safian

Simple cuboidal

Mostafa Safian

Simple cuboidal

Mostafa Safian

Simple columnarepithelium

-structure :single layer of epithelial cells.

the cells appear tall and narrow

( their height is greater than their width )

their nuclei are oval and located in the basal half of the cell

-occurrence :In large intestine

and stomach

Mostafa Safian

Pseudostratified columnar epithelium

-structure :it's so, called because

the nuclei are seen at different levels

but all the cells rest on the basement membrane

and not all the cells reachto the surfaceconsist of:

1-tall cell, with proximally located nucleus

2-short cell, with distally located nucleus

-occurrence :Epididymis

Mostafa Safian

Pseudostratified columnar epithelium

Mostafa Safian

Pseudostratified columnar epithelium

Mostafa Safian

Pseudostratified columnar epithelium

Mostafa Safian

Pseudostratified columnar epithelium

Mostafa Safian

transitional epithelium

-structure :1-basal cell layer:

one layer of cuboidal cells2-middle layer :

several layer of polyhedral cells toward the base

and bear shaped with their bases

toward the surface3-superfescial layer :

cuboidalcells with convex surface

(umbrella -dome shaped cells)with (1-2) nuclei

-occurrence :Urinary bladder,

ureter, urethra

Mostafa Safian

transitional epithelium

Mostafa Safian

transitional epithelium

Mostafa Safian

transitional epithelium

Mostafa Safian

transitional epithelium

Mostafa Safian

stratified squamous epithelium

-structure :1-basal layer :

one layer of columnar or cuboidal 2-middle layer:

several layers of irregular polyhedral cells with

rounded nuclei3-superfescial layer :

squamous

-occurrence :A-cornified: skinB- non-cornified: oesophagus

Mostafa Safian

non-cornified

stratified squamous epithelium cornified

Mostafa Safian

non-cornified

stratified squamous epithelium cornified

Mostafa Safian

non-cornified

stratified squamous epithelium cornified

Mostafa Safian

non-cornified

stratified squamous epithelium cornified

Mostafa Safian

non-cornified

stratified squamouscornified

Mostafa Safian

cornified

stratified squamouscornified

Mostafa Safian

cornified

Mucous gland

-occurrence:

palatine salivary glands oesophageal glands of dogs

Mostafa Safian

Mucous gland

Mostafa Safian

Serous gland

-occurrence:

pancreas, parotid salivary glands

Mostafa Safian

Mixed gland

-occurrence:

mandibular salivary glands

Mostafa Safian

Apocrine gland

-occurrence:

Mammary gland

Mostafa Safian

Apocrine gland

Mostafa Safian

Apocrine gland

Mostafa Safian

Apocrine gland

Mostafa Safian

Apocrine gland

Mostafa Safian

Apocrine gland

Mostafa Safian

Holocrine gland

-occurrence:

sabaceous gland

Mostafa Safian

Holocrine gland

Mostafa Safian

Holocrine gland

Mostafa Safian

Holocrine gland

Mostafa Safian

Holocrine gland

Mostafa Safian

أسأل الله التوفيق لي وللجميعمصطفى صفيــان أخوكم: