BioTecNika’s Model Test Papers for

CSIR NET Life Sciences

June 2013 Examination

Practice Today with the best Model Papers.

Get to know where you stand in the competition

Model Test Paper - 3

Model Test Paper - 3

PART-A

1. In June a baseball team played 60 games

and won 30% of them. After a phenomenal

winning streak this team raised its average to

50%. How many games must the team have

won in a row to attain this average?

a. 30

b. 24

c. 12

d. 20

e. 26

2. There are 200 questions in a 3 hr

examination. Among these questions are 50

mathematics problems. It is suggested that

twice as much time be spent on each maths

problem as for each other question. How

many minutes should be spent on

mathematics problems?

a. 36

b. 72

c. 60

d. 100

e. 64

3. In a group of 15 students, 7 students have

studied Latin, 8 students have studied Greek,

and 3 have not studied either. How many of

these studied both Latin and Greek

a. 3

b. 5

c. 4

d. 0

e. 2

4. Which letter represents the question mark?

a. L

b. M

c. O

d. R

5. A boat can travel with a speed of 13 km/hr

in still water. If the speed of the stream is 4

km/hr, find the time taken by the boat to go

68 km downstream.

a. 2 hours

b. 3 hours

c. 4 hours

d. 5 hours

6. Present ages of Sameer and Anand are in

the ratio of 5 : 4 respectively. Three years

hence, the ratio of their ages will become 11

: 9 respectively. What is Anand's present age

in years?

a. 24

b. 27

c. 40

d. Cannot be determined

e. None of these

7. P can run 100m in 20 seconds and Q in 25

seconds. P beats Q by

a. 10 m

b. 20 m

c. 25 m

d. 12 m

8. A bag contains 11 fruits of which 3 are

spoiled. If 4 fruits are chosen at random, find

the probability that all the fruits are spoiled.

a. 0

b. 1

c. 14/55

d. 4/165

9. The ratio of the circumferences of two circles

is 1/2 : 1/3. Find the ratio of their respective

areas.

a. 3:2

b. 4:9

c. 2:3

d. 9:4

10. You are a builder, who has taken a project

of building a wall against the falling mud hill.

Its height is 100m on one side and 125 on the

other end and width is 80 mts. The square

bricks used have side of 0.5 m. How many

bricks are required to build the wall, if only

one layer of brick is used?

a. 20000

b. 25000

c. 40000

d. 33000

11. The cost of 2 pen and 2 is 50/- , then wats

the cost of 5 pen and 5 pencil ?

a. 100

b. 150

c. 125

d. 75

12. At what rate percent will Rs.400 amount to

Rs.460 in 3 years?

a. 2

b. 4

c. 5

d. 6

13. In the afternoon, Berth read 100 pages at the

rate of 60 pages per hour; in the evening,

when she was tired, she read another 100

pages at the rate of 40 pagers per hour.

What was her average rate of reading for the

day?

a. 45

b. 48

c. 50

d. 52

14. Since 1950, when Dev was retired from the

team, he has gained 2 pounds every year. In

1980 he was 40% heavier than in 1950. What

percent of his 1995 weight was his 1980

weight?

a. 80

b. 85

c. 87.5

d. 90

15. What is the greater of two numbers whose

product is 900, if the sum of the two numbers

exceeds their difference by 30?

a. 15

b. 60

c. 75

d. 90

PART-B

1. Nutrient content and biological structures

are considered as

a. implicit factor for microbial growth

b. intrinsic factor for microbial growth

c. processing factor

d. none of the above.

2. Phophatidic acid derivative involved in the

activation of enzymes of blood clotting

mechanism is

a. Phosphatidylcholine

b. Phosphatidylserine

c. Phosphatidyl ethanolamine

d. Phosphoglyceride.

3. The membrane of lysosome is protected

from its enclosing hydrolytic enzymes by

a. High unusual glycosylation of

membrane protein.

b. Addition of unusual glycolipid.

c. The protein in the membrane in the

inner leaflet contain many

hydrophobic amino acids.

d. Inner side of the membrane contain

a layer of mucous which protect

them from degrading enzymes.

4. Aldosterone is synthesized in

a. Zona glomerulosa

b. Zona Fasiculata

c. Zona reticularis

d. Chromaffin cells

5. Diabetogenic effect is seen because of

a. hGH

b. prolactin

c. Serotonin

d. Melatonin

6. In myopia

a. Image is not formed

b. Image is formed in front of the retina

c. Image is formed behind retina

d. Image is formed on the retina

7. This region is characterized by large plant

eating mammals like lions and hyenas and

wildebeests and zebras a. Savanna Region

b. Temperate Grassland

c. Tropical Rain Forest

d. Taiga Region

8. Calculate the number of births per year if the

annual per capita birth rate is 0.034 and the

population size is 500

a. 20 per year

b. 17 per year

c. 16 per year

d. 10 per year

9. In 2009, the United States had a population

of about 307 million people. If there were 14

births and 8 deaths per 1,000 people, what

was the country’s net population growth that

year?

a. 1.74million

b. 1.94 million

c. 1.84million

d. 1.24 million

10. A mutation is introduced in the Lex A gene

such that autocatalytic cleavage of Lex A

protein is prevented. How will the SOS

response in this type of cell be affected?

a. The genome will be repaired with

high fidelity.

b. The cells become more sensitive to

DNA damage.

c. RecA concentration increases,

irrespective of the concentration of

LexA protein.

d. SOS genes will be induced to a high

degree.

11. The antibiotic which acts by interfering with

one or more steps in protein glycosylation is

a. Streptomycin

b. Vancomycin

c. Erythromycin

d. Tunicamycin

12. When histone H1 is incorporated into a

nucleosome

a. the nucleosome is about to be

moved by a remodeling enzyme.

b. it means that replication of the DNA

has just occured.

c. it increases the probability that

condensation of the chromatin will

occur.

d. it means that the underlying DNA is

probably a core promoter.

13. In both C4 and CAM plants, PEP carboxylase

is

a. Inhibited by malate and activated

by glucose-6-phosphate

b. Inhibited by pyruvate and activated

by CO2.

c. Inhibited by CO2 and activated by

pyruvate.

d. Inhibited by Glucose-6-phosphate

and activated by malate.

14. “Agent orange” the leaf defoliator used by

USA in Vietnam war was

a. Ethylene

b. 2,4-D and 2,4,5-T

c. 2,4-D and NAA

d. 2,4,5-T and NAA

15. If stomata are present at upper surface of

leaf, the condition is called or leaf is called

a. Hystomatic

b. Hypostomatic

c. Epistomatic

d. Amphistomatic

16. Human height is an example of

a. Qunalitative trait

b. Pleotropy

c. Continous trait

d. Discontinous trait

17. What is the resultof cross involving a

suppressor mutant form and a normal wild

type palnt.

a.

b. 2 parental and 2 recombinant

c. All are mutants

d. 3 mutant and 1 wild type

e. All wild type

18. In an ordered neurospora crossing, 52

gametes were found to be different from

parental one. What is the recombination

frequency , if total 152 gametes were

studied?

a. 0.34

b. 0.17

c. 0.56

d. 0.89

19. In which period , the Himalayan mountains

formed?

a. Pleistocene epoch

b. Eocene epoch

c. Oligocene epoch

d. Jurassic period

20. Which of the following anatomical region of

brain is involved in animal behavior like

aggression, attack, fight and submission

a. Pitutory gland

b. Hypothalamus

c. Frontal lobe

d. CNS

21. Biological clock in human is controlled by

a. Nucleus Suprachiasmaticus

b. Hypothalamus

c. Central nervous system

d. retina

22. Genotypically XX mice that carry a

transgene for SRY would:

a. develop testes

b. develop ovaries

c. develop both testes and ovaries

d. form testes with primordial follicles in

the tubules

23. If the frog embryos are injected with a

morpholino (molecule used to modify gene

expression) against fibronectin. Which of the

following developmental processes will be

affected:

a. neural tube closure

b. migration of cells during gastrulation

c. the first cell division will be blocked

d. the first nuclear division will be

blocked

24. The placenta is formed from the fetal portion

of?

a. The epiblast

b. The trophoblast and some mesoderm

c. The allantois and yolk sac

d. The endometrium

25. If you were shipping strawberries to a

supermarket thousands of miles away, which

of the following chemicals would you want

to eliminate from the plants environment?

a. cytokinins

b. ethylene

c. auxin

d. gibberellic acids

26. You are part of a desert plant research team

trying to discover crops that will be

productive in arid climates. You discover a

plant that produces a guard cell hormone

under water-deficit conditions. Most likely

the hormone is

a. ABA.

b. GA.

c. IAA.

d. 2, 4-D.

27. In most dicot seeds, which of the following

structures is responsible for the storage of

carbohydrates?

a. Seed coat

b. Cotyledon

c. Radicle

d. Embryo

28. If a restriction endonuclease recognizes the

sequence GATGCT and cuts the DNA after

the first G in the recognition sequence, what

overhang will be present on the ends of the

DNA produced at the cut site?

a. 3 nucleotide long 5' overhangs

b. 4 nucleotide long 5' overhangs

c. 3 nucleotide long 3' overhangs

d. 4 nucleotide long 3' overhangs

29. If a 3300 bp circular plasmid can be cut in

five locations by a restriction endonuclease,

and these locations are at map locations 0,

500, 800, 1900, and 2300, which of the

following fragments is not possible during a

partial digest with this same restriction

endonuclease?

a. 1000 bp

b. 1900 bp

c. 1100 bp

d. 400 bp

30. If the increase the annealing step

temperature in typical PCR reaction,

a. false priming is more likely

b. primer-dimer formation is more likely

c. inefficient amplification is likely

d. poor processivity is likely

31. If a protein has a pI of 5, what charge would

it have in buffer solution of pH 7.6?

a. No charge

b. Net positive charge

c. Cant predict

d. Net negative charge

32. A homo-polymeric protein has got a pI of

3.2. What type of amino acids you can

expect in this protein?

a. Only positively charged a.a

b. Only negatively charged a.a

c. Both in equal numbers

d. Uncharged a.a

33. Nonregular, nonrepetitive secondary

structures

a. Include α-helices and β-sheets

b. Are bends, loops, and turns that do

not have a repeating element

c. Are beta-strands

d. Are Beta-sheets

e. Include beta-sheets

34. Motifs are supersecondary structures. Any

motif can be defined by describing:

a. The first 50 to 100 amino acids in the

primary structure

b. Any secondary structure and the turn

at its C-terminus

c. Any secondary structure and the

loop at its N-terminus

d. Any two secondary structures in the

protein

e. A group of secondary structures and

their relationship to one another

35. In order for H-Ras to be functional, it needs to

associate with the cell membrane. This

process involves which post-translational

modification?

a. Glycosylation

b. Hydroxylation

c. Farnesylation

d. Dephosphorylation

PART-C

1. S-phase cyclins and CDKs begin to

accumulate early in G1 phase but the DNA

replication is seen only in S-phase. The

reason for this is

a. The S-cyclin and CDKs are inhibited

by Sic1, phosphorylation of which

marks the beginning of S-phase.

b. Activation of S-cyclins and CDKs

requires phosphorylation which

occurs during G1-S transition.

c. Dephosphorylation of S-cyclins is

required for G1-S transition.

d. None of the above

2. Nuclear lamina was isolated from blood

sample taken from a patient. The lamina

obtained from each formed element (WBC,

RBC and Platelets) of the blood was run on

gel-electrophoresis. Two bands were only

observed instead of three. Which of the

following conclusion is correct?

a. The absence of third band confirmed

that the patient is suffering from

laminopathy.

b. The absence of third band

concluded that one of the elements

lacked nuclear lamina.

c. Experiment needs to be conducted

again as one was sample did not

have any band.

d. Nothing can be concluded from this

experiment.

3. Match the following

Column A Column B

1. Myopia A. Farsightedness

2. Hypermetropia B. Nearsightedness

3. emmetropia C. Irregular curvature

4. astigmatism D. Normal vision

a. 1-B, 2-A, 3-D, 4-C

b. 1-D, 2-C, 3-A, 4-B

c. 1-D, 2-A, 3-D, 4-B

d. 1-B, 2-C, 3-D, 4-A

4. The correct sequence of activation of

clotting factors during intrinsic pathway of

blood clotting is

a. Factor XII, factor X, factor II, factor-XIII

b. Factor X, factor-XII, factor-XIII, factor-

II

c. Factor -II, Factor -X, Factor -XII, Factor

XIII

d. Factor -XIII, Factor II, Factor X, Factor-

XII

5. W. J. Fletcher, of the University of Sydney,

Australia, reasoned that if sea urchins are a

limiting biotic factor in a particular

ecosystem, then more seaweeds should

invade an area from which sea urchins have

been removed. To isolate the effect of sea

urchins from that of a seaweedeating

mollusc, the limpet, he removed only

urchins, only limpets, or both from study

areas adjacent to a control site. Fletcher

observed a large difference in seaweed

growth between areas with and without sea

urchins.What conclusion can be drawn from

the experiment?

a. Removing both limpets and urchins

resulted in the greatest increase in

seaweed cover, indicating that both

species have some influence on

seaweed distribution

b. Sea urchins have a much greater

effect than limpets in limiting

seaweed distribution

c. Both a) and b)

d. Removal of limpets have the same

effect on Seaweed distribution as is

the removal of sea urchins

6. The Glanville fritillary (Melitaea cinxia)

illustrates the movement of individuals

between populations. This butterfly is found

in about 500 meadows across the Åland

Islands of Finland, but its potential habitat in

the islands is much larger, approximately

4,000 suitable patches.Explain the

metapopulation concept using the above

example

a. New populations of the butterfly

regularly appear and existing

populations become extinct,

constantly shifting the locations of

the 500 colonized patches

b. The species persists in a balance of

extinctions and recolonizations.

c. All Granville butterfly are uniformly

distributed in all the habiats so that

they are prevented from getting

extincted

d. Both a) and b)

7. An archaebacterium (Sulfolobus

acidocaldarius) found in acidic hot springs

contains a topoisomerase that catalyzes the

ATP-driven introduction of positive supercoils

into DNA. Most organism genome are

negatively supercoiled. The advantage of

this enzyme to this particular organism can

be due to which of the following reasons?

a. Positive Supercoiling, at high

temperature, eases the unwinding of

DNA for replication.

b. Positive Supercoiling increases the

melting temperature of DNA.

c. Positively Supercoiled DNA is more

compact than negatively

supercoiled DNA of the same length.

d. The topoisomerase that introduces

Positive supercoiling is more stable at

high temperature than

topoisomerase that introduces

negative supercoiling.

8. A thalassemic patient had a mutation

leading to the production of an mRNA for the

β-chain of hemoglobin that was 900

nucleotides longer than the normal one. The

poly A tail of this mutant mRNA was located

a few nucleotide after the only AAUAAA

sequence in the additional sequence. The

most likely reason for this altered mRNA is?

a. A mutation in the first AAUAAA

sequence of mRNA.

b. A mutation in the DNA converting a

amino acid coding codon to a stop

codon.

c. A mutation in the splice acceptor site

of one of the introns in the mRNA.

d. A mutation missense mutation in DNA

sequence, altering an amino acid

encoding codon.

9. Ribosomes were isolated from bacteria

grown in heavy medium (13C and 15N) and

from bacteria grown in light medium (12C

and 14N). These 70 S ribosomes were added

to an in vitro system actively engaged in

protein synthesis. An aliquot removed

several hours later was analyzed by density

gradient centrifugation. How many bands of

70S ribosome would you expect to see in the

density gradient?

a. 1

b. 2

c. 3

d. 4

10. Carotenoids are important accessory

pigments involved in exciton transfer to the

reaction center. But they also play an

important role in photoprotective

mechanisms. The photoprotection

mechanism can be thought of as a safety

valve, venting excess energy before it can

damage the organism. How are caroteniods

involved in photoprotection in plants?

a. Carotenoids are involved in

quenching of chlorophyll excited

state, which would otherwise form

singlet oxygen species.

b. Release of excess of energy in the

form of fluorescence

c. Chromoplasts, which are carotenoid

containing plastids, are responsible

for dissipation of excess of energy as

heat.

d. Carotenoids help in exciton transfer

to other accessory pigment

molecules, inhibiting photooxidation.

11. Leaves can regulate the amount of incident

light that can be absorbed by regulating the

orientation and location of chloroplast in the

leaves. Consider the following statements:

A. Under low light, chloroplasts gather at

cell surfaces and align themselves in the

plane of the leaf, perpendicular to the

incident light.

B. Under high light conditions, chloroplasts

gather at cell surfaces and arrange

themselves parallel to the incident light.

C. Chloroplast movement in leaves is a

typical blue-light response.

D. Chloroplasts move along actin

microfilaments in the cytoplasm.

The correct statements are

a. A and B only

b. A, B and C only

c. A, B and D only

d. All the above statements are correct

12. The data is from Mendel’s experiments: Of

the 565 plants raised from F2 round seeded

plants, 372 gave both round and wrinkled

seeds in 3:1 proportion, where as 193 yielded

only round seeds when self fertilized. It was

hypothesized that it does follow mendelism.

Test the hypothesis with (chi)2 test.

a. Hypothesis is accepted

b. Hypothesis is rejected

c. Can’t say

d. Hypothesis can be rejected but not

sure.

13. Mr.Dumdum was experimenting on nucleic

acids. He isolated a nucleotide X in the test

tube and wants to find out its identity. On

addition of NH2OH , he could find formation

of white ppt of Hydroxylamine cytosine ; on

addition of HNO2 , he found it to produce

uracil, which did not showed

Transesterification reaction. Wht he might

have concluded about the compund X?

a. Adenine

b. Uracil

c. Guanine

d. Cytosine

14. In a population frequency of recessive allele

is found to be 0.3 . The fitness of the recessive

homozygotes is 0.4 . What will be the number

of homozygous recessive individuals after

selection, in one generation?

a. 9%

b. 0.09%

c. 0.036%

d. 3.6%

15. It is a very common to find the dogs,

urinating frequently and smelling as they

move along. What may be the reason for

this?

a. In search of mate

b. In search of food

c. Marking its territory and directions

d. Dogs sense the pheramones in urine

and detect the food nearby.

16. In plants, sexual reproduction

I. involve gametes formation and

fertilization

II. occurs through formation of sexual

spores

III. occurs through formation of meiospores

IV. produce genetically identical offspring

a. I and II

b. II, III and IV

c. I, II and III

d. I, II and IV

17. In human during gastrulation, heart

development begins as:

a. a folding process in the mesoderm

below the notochord that is exactly

like neural tube formation.

b. an outgrowth of the endoderm

above the gut that forms a tube that

will become the heart.

c. a pair of tubes in the lateral plate

mesoderm that move together and

fuse to form a single tube that will

become the heart.

d. a mesenchymal condensation below

the notochord that forms the heart

tube.

18. Match the following.

Column A Column B

I. Somaclonal

variation

A. anther culture

II. Haploid culture B. Suspension culture

III. Protoplast

isolation

C. Callus culture

IV. Secondary

metabolite

D. cell wall

degrading enzymes

a. I C, II A, III D, IV B

b. I A, II B, III C, IV D

c. I B, II A, III D, IV C

d. I B, II D, III A, IV C

19. Match the following.

Column

A

Column B

I. RAPD A. detected fragment varies

between individuals

II. AFLP B. personal identification

III. RFLP C. used to analyse the genetic

diversity

IV. VNTR D. quantitative trait locus analysis

a. I C, II D, III A, IV D

b. I D, II C, III A, IV D

c. I B, II A, III D, IV C

d. I B, II D, III A, IV C

20. Which of the following are the characteristics

of type II restriction endonucleases?

I. Nature of enzyme : Bifunctional enzyme

with both endonuclease and methylase

activity

II. Protein structure : Two identical subunits

III. Restriction requirement : ATP, Mg2+

IV. Cleavage sites : At or near restriction site

a. I and II

b. II and IV

c. I and IV

d. III and IV

21. You are studying a virus called PK15, which

is known to cause skin tumors in mice. The

genome of PK15 is a 3.3 kb, double-

stranded, linear DNA molecule. You are

interested in learning more about PK15, so

you decide to map its DNA using restriction

enzymes. As a first step in your analysis, you

digest PK15 DNA with three enzymes: SspI,

XhoI, and SmaI. You run the digested DNA

on an agarose gel, along with an uncut

sample of the PK15DNA as a control. You

obtain the following pattern of bands. How

many times does SspI and Smai cut the pK15

DNA?

a. 5 and 4 times

b. 4 and 3 times

c. 4 and 5 times

d. 3 and 4 times

22. If aminopterin, a potent inhibitor of

dihydrofolate reductase, is added ao a

culture of growing cells, which major

biosynthetic process will be inhibited first?

a. Carbohydrate synthesis

b. Lipid synthesis

c. DNA synthesis

d. RNA synthesis

23. In the presence of a compound X, protein

synthesis can initiate, but only dipeptides are

formed that remain bound to the ribosome.

Therefore, compound X affects eukaryotic

protein synthesis by blocking

a. The activity of elongation factors

b. The activation of amino acids

c. The recognition of stop signals

d. The formation of peptide bonds

24. Active form of insulin contains

a. Two inter-chain disulphide bonds

b. Two intra-chain and one inter-chain

disulphide bonds

c. One intra-chain and two inter-chain

disulphide bonds

d. Two intra-chain disulphide bonds

25. Which amongst the following is not a

characteristic of gap juctions?

a. They allow small ions and molecules

to migrate from a cell an adjoining

cell

b. They are constituted of a class of

membrane associated proteins

named connexion

c. They are found only in plant cells

d. They are channel like structures

connecting the cytoplasm of

adjoining cells

-- End of Questions –

BioTecNika starting its

Next batch of 6 Month

Regular Classroom and

Online Live Class Coaching

from 12th and 14th July 2013.

Hurry Only Limited Seats Available

Enrol Today!!

For Enquiries Contact:

080-32494384 / 080-65705331

Answers

PART-A

1. B

2. B

3. A

4. C

5. C

6. A

7. B

8. A

9. D

10. C

11. C

12. C

13. B

14. C

15. B

PART-B

1. B

2. B

3. A

4. A

5. A

6. B

7. A

8. B

9. C

10. B

11. D

12. C

13. A

14. B

15. C

16. C

17. A

18. B

19. b

20. B

21. A

22. A

23. B

24. B

25. B

26. A

27. B

28. B

29. D

30. C

31. d

32. b

33. b

34. e

35. c

Part-C

1. A

2. b

3. a

4. a

5. c

6. d

7. b

8. a

9. d

10. a

11. d

12. b

13. d

14. d

15. c

16. c

17. c

18. a

19. a

20. b

21. b

22. c

23. a

24. c

25. c

BioTecNika starting its

Next batch of 6 Month

Regular Classroom and

Online Live Class Coaching

from 12th and 14th July 2013.

Hurry Only 15 Seats per Batch

Enrol Today!!

For Enquiries Contact:

080-32494384 / 080-65705331