csf: how certain can we be? meira louis pgy1. objectives present a published case highlighting the...
TRANSCRIPT
![Page 1: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/1.jpg)
CSF: How certain can we be?
Meira Louis
PGY1
![Page 2: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/2.jpg)
Objectives
• Present a published case highlighting the difficulties in CSF diagnosis
• Understand the objective evidence for the tests ordered on CSF
• Understand where clinical judgement falls in the spectrum of certainty
![Page 3: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/3.jpg)
Sheila21yo female
1 day history:– non-specific lethargy– Fever and rigors– Generalized headache– Nausea, vomiting
PMX:– Childhood asthma– Hyperthyroidism
Meds:– None
Huynh et al, 2007
![Page 4: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/4.jpg)
• On exam:– Vitals: 38°C– Alert, oriented– Normal neuro
• Bloodwork– WBC: 19.5– CRP: 185– Lytes, LFTs,
glucose
• Imaging:– Chest X-ray– Urinalysis– CT head
![Page 5: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/5.jpg)
CSF – for what?
• Cell count• Gram’s Stain• Turbidity• Xanthochromia• Glucose• Protein• India Ink• Cryptococcal Antigen• Lactic Acid• Bacterial Antigen tests• Acid Fast Stain
![Page 6: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/6.jpg)
Sheila’s CSF
• Clear and colourless
• Protein: 0.38 mg/dL
• Glucose: 3.6 mmol/L
• 12x106 RBC
• 1x106 WBC (all mononuclear)
• Negative gram stain
![Page 7: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/7.jpg)
What would you do?
• What’s your diagnosis?
• How confident are you?
• How confident should you be?
![Page 8: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/8.jpg)
Cell Count and Differential
• How many leuks are too many leuks?
• Does it matter what kind?– Monomorphic vs polymorphic– lymphocytosis
• Does prior abx change your cell count?
Thomson et al, 2001.; Van de Beek, 2004.
![Page 9: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/9.jpg)
What happens with a traumatic tap?
Predicted WBC = CSF RBC x serum WBC
serum RBC
• If WBC was more than 10x normal was 48% predictive of bacterial meningitis
• If less than 10x was 99% predictive of it NOT being meningitis
Mayefsky et al. 1987
![Page 10: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/10.jpg)
Glucose
• Hypoglycorrhachia
• If normal serum glucose:– Ratio of CSF:serum is 0.6:1– Abnormal when less than 0.5
• If elevated serum glucose:– Ratio of CSF:serum is 0.4:1– Abnormal when less than 0.3
![Page 11: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/11.jpg)
Protein
• Normal range in CSF: 15-45 mg/dL– Greater than 150 is probably bacterial– Greater than 1000 should suggest fungal
• Other causes?– Any meningitis– Subarachnoids– CNS vasculitis– Syphilis– Viral encephalitis– neoplasms
![Page 12: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/12.jpg)
Gram Stain
All common etiologies-no previous antibiotics 75-90%All common etiologies-antimicrobial therapy prior to LP 40-60%Streptococcus pneumoniae 90%Neisseria meningitidis 75%Haemophilus influenzae 86%Listeria monocytogenes <50%Gram-negative bacilli 50%
What’s the sensitivity for bacteria?
Gray et al, 1992
![Page 13: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/13.jpg)
Other tests
Lactic Acid• Non-specific• Elevations over 35 mg/dL may indicate
bacterial meningitis• Lactate may rise before glucose dropsSerum Procalcitonin• Very sensitive • Not available for up to 24 hours
![Page 14: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/14.jpg)
Back to the case…
• The following morning:– Diplopia, worsening headache– Temp increase to 40°C– GCS of 9– No rash, no nuchal rigidity, no focal neuro
• Repeat CT scan with contrast• IV ceftriaxone, gentamicin, and acyclovir were
started• Blood and CSF came back positive for N.
meningitidis
![Page 15: CSF: How certain can we be? Meira Louis PGY1. Objectives Present a published case highlighting the difficulties in CSF diagnosis Understand the objective](https://reader036.vdocuments.site/reader036/viewer/2022070413/5697bfbc1a28abf838ca1a6c/html5/thumbnails/15.jpg)
On a reassuring note…
Sensitivity Specificity Accuracy
ER doc 0.89 0.77 0.79
CSF leuk 0.50 0.94 0.71
Glucose 0.33 0.42 0.11
Protein 0.63 0.94 0.75
CRP 0.78 0.74 0.75
Procalcitonin 0.87 1.00 0.99
Ray et al, 2006