correction

Correction 2010. Defining the Limits of Single-Molecule FRET Resolution in TIRF Microscopy . Holden SJ, Uphoff S, Hohlbein J, Yadin D, Le Reste L, Britton OJ, and Kapanidis AN. Biophys J. 99:3102–3111. We have identified an error in our manuscript Holden et. al, 2010, Biophys. J. Eq. 6 (also referred to as Eq. S15) was given as: sðEÞ¼ ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi f 2 G E 0 ð1 E 0 Þ N þ 4p a 2 N 4 D 2 s 2 D b 2 D þ A 2 s 2 A b 2 A r : In this equation, the donor and acceptor photon count terms, D and A respectively, were erroneously switched. Eq. 6 should read: sðEÞ¼ ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi f 2 G E 0 ð1 E 0 Þ N þ 4p a 2 N 4 A 2 s 2 D b 2 D þ D 2 s 2 A b 2 A r : The same error affects Eq. S22, which should read: s ap ðEÞ¼ ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi f 2 G E 0 ð1 E 0 Þ N þ 1 N 4 A 2 a D b 2 D þ D 2 a A b 2 A r : All results apart from those presented in Fig. 2A and 2B used the numerically in-tegrated version of the theoretical predictions, Eq. S14, and are therefore unaffected. However, the red lines on Fig. 2A and 2B do use Eq. 6. Symmetry at FRET efficiency of 0.5 means that Fig. 2A requires no correction. A corrected Fig. 2B, showing both the incorrect and corrected theoretical predictions (red line and magenta line respectively), is presented below. For the parameters used in the calculations for Fig. 2B, the theoretical prediction of Eq. 6 is changed by less than 2.5% in the range 0.2 % E0 % 0.8 and less than 5% over the entire calculated range, 0.1 % E0 % 0.9. Therefore none of the conclusions of the manuscript are affected by this error. We note also a typographical error, which does not affect any of the calculations or conclusions of the manuscript: the negative exponent of the Gaussian function in Equations 4, 5, 7, and S7 was erroneously omitted. http://dx.doi.org/10.1016/j.bpj.2014.03.030 B Corrected Figure 2B: Red line, incorrect theoretical prediction; magenta line, corrected theoretical prediction 2082 Biophysical Journal Volume 106 May 2014 2082

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2082 Biophysical Journal Volume 106 May 2014 2082

Correction

2010. Defining the Limits of Single-Molecule FRET Resolution in TIRF Microscopy. Holden SJ, Uphoff S, Hohlbein J,Yadin D, Le Reste L, Britton OJ, and Kapanidis AN. Biophys J. 99:3102–3111.

We have identified an error in our manuscript Holden et. al, 2010, Biophys. J. Eq. 6 (also referred to as Eq. S15) was given as:

sðEÞ ¼ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffif 2GE0ð1� E0Þ

Nþ 4p

a2N4

�D2s2Db

2D þ A2s2Ab

�r:

In this equation, the donor and acceptor photon count terms, D and A respectively, were erroneously switched. Eq. 6should read:

Nþ 4p

a2N4

�A2s2Db

2D þ D2s2Ab

�r:

The same error affects Eq. S22, which should read:

sapðEÞ ¼ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffif 2GE0ð1� E0Þ

Nþ 1

�A2aDb2D þ D2aAb2A

�r:

All results apart from those presented in Fig. 2A and 2B used the numerically in-tegrated version of the theoreticalpredictions, Eq. S14, and are therefore unaffected. However, the red lines on Fig. 2A and 2B do use Eq. 6.

Symmetry at FRET efficiency of 0.5 means that Fig. 2A requires no correction. A corrected Fig. 2B, showing both theincorrect and corrected theoretical predictions (red line and magenta line respectively), is presented below. For the parametersused in the calculations for Fig. 2B, the theoretical prediction of Eq. 6 is changed by less than 2.5% in the range 0.2% E0%0.8 and less than 5% over the entire calculated range, 0.1 % E0 % 0.9. Therefore none of the conclusions of the manuscriptare affected by this error.

We note also a typographical error, which does not affect any of the calculations or conclusions of the manuscript: thenegative exponent of the Gaussian function in Equations 4, 5, 7, and S7 was erroneously omitted.

Corrected Figure 2B: Red line, incorrect theoretical prediction; magenta line, corrected theoretical prediction

http://dx.doi.org/10.1016/j.bpj.2014.03.030

http://crossmark.crossref.org/dialog/?doi=10.1016/j.bpj.2014.03.030&domain=pdf

http://dx.doi.org/10.1016/j.bpj.2014.03.030

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