core assay list 2011
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Natural Remedies offers in vitro assay servicesTRANSCRIPT
In Vitro Cell Based assay List
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
CORE ASSAY LIST 2011
THERAPEUTIC AREA – IMMUNOMODULATION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA01 Cell
Proliferation
Test model: Determine effect of mitogens/inhibitors on cell
proliferation in murine splenocytes/Human PBMC. Useful for lead
optimization of proliferation/ anti-proliferation compounds.
Assay format: Incubations in 48-well plates. MTT read out.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA02 Mediator
release
Test model: Determine effect of compounds on (un-stimulated)
Nitric Oxide release from murine macrophages. Useful for lead
optimization for pro-inflammatory compounds and potency
determination.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA03 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-2) from murine splenocytes. Useful for lead
optimization for pro-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA04 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-3) from murine splenocytes. Useful for lead
optimization for pro-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – IMMUNOMODULATION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA05 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-4) from murine splenocytes. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA06 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-5) from murine splenocytes. Useful for lead
optimization for pro-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA07 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-6) from murine splenocytes. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA08 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-10) from murine splenocytes. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – IMMUNOMODULATION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA09 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-12) from murine splenocytes. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
CA10 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IFN-γ) from murine splenocytes. Useful for lead
optimization for pro-inflammatory compounds and potency
determination.
Assay format: Incubations in 48-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 3 compounds at
5 concentrations tested
in quadruplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA11 Mediator
release
Test model: Determine effect of compounds on (stimulated) Nitric
Oxide release from murine macrophages. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA12 Mediator
release
Test model: Determine effect of compounds on (stimulated)
Prostaglandin release (PGE2) from murine macrophages. Useful
for lead optimization for Anti-inflammatory compounds and
potency determination.
Assay format: Incubations in 96-well plates. HTRF.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA13 Mediator
release
Test model: Determine effect of compounds on (stimulated)
Thromboxane release (TXB2) from Human pro-myelocytic
leukemia cells. Useful for lead optimization for Anti-inflammatory
compounds and potency determination.
Assay format: Incubations in 96-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA14 Mediator
release
Test model: Determine effect of compounds on (stimulated)
Leukotriene release (LTB4) from Human pro-myelocytic leukemia
cells. Useful for lead optimization for Anti-inflammatory
compounds and potency determination.
Assay format: Incubations in 96-well plates. HTRF.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – ALLERGY/PAIN AND INFLAMMATION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA15 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (IL-1β) from murine macrophages. Useful for lead
optimization for Anti-inflammatory compounds and potency
determination.
Assay format: Incubations in 96-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA16 Mediator
release
Test model: Determine effect of compounds on (stimulated)
Histamine release from rat basophilic leukemia cells. Useful for
lead optimization for Anti-allergic compounds and potency
determination.
Assay format: Incubations in 96-well plates. HTRF.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA17 Mediator
release
Test model: Determine effect of compounds on (stimulated)
cytokine release (MCP-1) from murine peritoneal macrophages.
Useful for lead optimization for Anti-inflammatory compounds and
potency determination.
Assay format: Incubations in 96-well plates. ELISA.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA18 In vitro
Toxicity
Test model: Measurement of compound toxicity in hepatocytes
against t-BH using viability (MTT) endpoints. Assess compound
safety before proceeding to clinical development.
Assay format: Incubations in 96-well plates. In vitro toxicity
profiling.
Data format: Word™ report with data available in an Excel™ file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA19 In vitro
Toxicity
Test model: Measurement of compound toxicity in hepatocytes
against diazinon (OPC) using viability (MTT) endpoints. Assess
compound safety before proceeding to clinical development.
Assay format: Incubations in 96-well plates. In vitro toxicity
profiling.
Data format: Word™ report with data available in an Excel™ file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA19 In vitro
Toxicity
Test model: Measurement of compound toxicity in hepatocytes
against Aldrin (OC) using viability (MTT) endpoints. Assess
compound safety before proceeding to clinical development.
Assay format: Incubations in 96-well plates. In vitro toxicity
profiling.
Data format: Word™ report with data available in an Excel™ file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA20 In vitro
Toxicity
Test model: Measurement of compound toxicity in hepatocytes
against Aflatoxin (AFB1) using viability (MTT) endpoints. Assess
compound safety before proceeding to clinical development.
Assay format: Incubations in 96-well plates. In vitro toxicity
profiling.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
Data format: Word™ report with data available in an Excel™ file.
THERAPEUTIC AREA – IN VITRO TOXICITY- HEPATOPROTECTION
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA21 In vitro
Toxicity
Test model: Measurement of compound toxicity in hepatocytes
against Galactosamine using viability (MTT) endpoints. Assess
compound safety before proceeding to clinical development.
Assay format: Incubations in 96-well plates. In vitro toxicity
profiling.
Data format: Word™ report with data available in an Excel™ file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
THERAPEUTIC AREA – COSMETOLOGY
REF# Project
Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA22 Anti-wrinkle
Test model: Determine effect of compounds on (un-stimulated)
collagen release from primary human dermal fibroblast. Useful for
lead optimization for cosmetology ingredient.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA23 Skin
whitening
Test model: Determine effect of compounds on (un-stimulated)
Melanin release from mouse melanoma cells. Useful for lead
optimization for cosmetology ingredient.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – DIABETES/OBESITY
REF# Project Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA24 GSIS Assay
Test Model: Measurement of induction of glucose stimulated
Insulin secretion in rat insulinoma cells. This approach is useful for
assessing the effectiveness of compounds under development for
the treatment of type 1 diabetes.
Assay Format: Incubations in 96-well plates. HTRF.
Data Format: WordTM report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA25
Lipid
Metabolism
Assays
(Adipogenesis)
Test model: Determine effect of compounds on (stimulated)
Adipocyte differentiation in Mouse embryonic fibroblast . Useful
for lead optimization for inhibitors of adipogenesis.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
CA26
Lipid
Metabolism
Assays
(Adipolysis)
Test model: Determine effect of compounds on (stimulated)
glycerol release in Mouse embryonic fibroblast . Useful for loead
identification for inhibitors of adipolysis.
Assay format: Incubations in 96-well plates. Colorimetric.
Data format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – ONCOLOGY
REF# Project Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA27 Anti
proliferative
assay
Test Model: Determine effect of compounds on cell proliferation
in human breast adenocarcinoma cells. Useful for lead optimization
of anti-cancer compound screening.
Assay Format: Incubations in 96-well plates. Colorimetric.
Data Format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
THERAPEUTIC AREA – GENERAL HEALTH/ HEART DISEASE
REF# Project Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA28 Antioxidant
assay
Test Model: Determine the cellular antioxidant potential of
compounds in Human hepatocellular liver carcinoma cells. Useful cost-effective method to evaluate the antioxidant activity of foods,
dietary supplements, phytochemicals and extracts..
Assay Format: Incubations in 96-well plates. Colorimetric.
Data Format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 4 compounds at
5 concentrations tested
in triplicate
R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.
Tel: 91-080-7832265, 7833092, 7834872, Fax: 91-080-7834369, E-mail: [email protected]
THERAPEUTIC AREA – IN VITRO GENOTOXICITY
REF# Project Type Assay Principle
Turn
Around
Time
Minimum
Compound
Required
Standard Format
CA29 Mutagenesis
assay
Test Model:. Determine the mutageneic potential of compounds
using 5 starins of S.Typhimurium with and without S9 fractions.
Quick assay to estimate the carcinogenic potential of a compound
Assay Format: Incubations in 384-well plates. Hightroughput.
Data Format: WordTM
report with data available in an ExcelTM
file.
4 Weeks 10 mg
Up to 1 compounds at
6 concentrations tested
in duplicate.
CA30 Mutagenesis
assay
Test model: Determine effect of compounds on the formation of
small membrane bound DNA fragments i.e. micronuclei in the
cytoplasm of interphase cells using Chinese hamster cells. Useful
for early identification of compounds likely to exhibit genotoxic
liability.
Assay format: Incubations in 90mm petri plates. Manual.
Data format: WordTM
report with data available in an ExcelTM
file.
15 Weeks 10 mg
Up to 1 compounds at
3 concentrations tested
in duplicate.
CA31 Cytogenetic
Assays
Test model: Determine effect of compounds on using induction of
structural changes in chromosomes Chinese hamster cells. Useful
to identify agents that cause structural chromosome aberration in
cultured mammalian cells.
Assay format: Incubations in 90mm petri plates. Manual.
Data format: WordTM
report with data available in an ExcelTM
file.
15 Weeks 10 mg
Up to 1 compounds at
3 concentrations tested
in duplicate.