Assay Development and Drug Repurposing Core

Drug Discovery Forum

Tom Dexheimer, Ph.D.Manager, MSU Assay Development and Drug Repurposing Core

May 14, 2015

MSU Assay Development and Drug Repurposing Core

• To provide equipment and expertise for high-throughput screening techniques to MSU researchers

• To provide MSU researchers with access to cost-effective chemical library screening for identification of molecules to further interrogate particular biological processes or potential leads for drug development (or drug repurposing)

• To promote a training environment that will expose students to the techniques of high-throughput assay design, development, and HTS screen execution

• To provide assistance and/or the generation of preliminary data for grant/funding opportunities

Goals of the ADDRC

Equipment – Liquid handling

Multidrop 384 Reagent Dispenser

• Peristaltic pump based cassette dispenser • Dispense 96- and 384-well plates• 5 to 395 mL volume range (5 mL

increments)

EL406 Microplate Washer Dispenser

• Peristaltic pump based cassette dispenser

• 1 mL and 5 mL cassettes• 96-well washer module• Syringe pumps dispensers• Variable dispense and

aspiration rates

Biomek FX Laboratory Automation Workstation• Automated/programmable robotic dual arm

system with 96-multichannel pipettor and Span-8 pipettor (8 individually controlled channels)

• Equipped with 384-pin tool• Small volume plate to plate transfers (i.e. compound plate to assay plate)• Volume range - 100 nL to 200 nL

Cytation 3 Cell Imaging Reader

Equipment – Readers

• Automated digital microscopy• Objectives: 4x and 20x (currently available)• Brightfield, 365 nm, 465 nm, 590 nm• Temperature and CO2/O2 control

Synergy Neo Multi-mode Reader• Combines filter-based optical system and monochromator optics,

providing both excellent performance and assay versatility• Detection modes: Fluorescence, time-resolved fluorescence,

fluorescence polarization, Alpha, luminescence, UV-visible absorbance

• Multi-plate stacker (30 plates)

Functional Drug Screening System FDSS/µCELL• Kinetic plate reader with an

integrated dispensing head and imaging-based detector

• High-speed digital CCD camera• 0.5 s to 100 s/data point • 480 nm /540 nm• 96- or 384-well compatible• Ca2+, cAMP, membrane

potential, etc.

Chemical Libraries and Informatics

GREENScreen• Integrated compound management and

high throughput screening data storage and analysis system

Chemical Libraries• 6571 compounds

MSU Drug Discovery

Drug Discovery listserv (DRUGDISCOVERY@List.msu.edu)• Bring together researchers from all disciplines at MSU who are interested

or involved in drug discovery and development

Drug Discovery Seminar Series• Fridays from 9:00 to 10:00 AM in B448 Life Science Building

• http://phmtox.msu.edu/core-facilities/Assay/DDIG.htm

Michigan Drug Discovery Symposium

• SAVE THE DATE September 9th-10th

Contact InformationTom Dexheimer, Ph.D. B411 Life Science BuildingEmail: dexheim1@msu.eduOffice: 517-884-4998

*The ADDRC facility was established through the generous support of the Vice President for Research and Graduate Studies, the Pharmacology and Toxicology Department, CNS, COM, CHM, CVM, and AgBioResearch.