colorimetric test instructions

6657 Frank Avenue NW Suite 200 North Canton, Ohio 44720 www.hydrodynesystems.com

Test Instructions

Colorimetric Methods


Dilution Factors

2 : 1 Dilution Factor

Mix 5 ml of distilled water with 5 ml of sample water together. Run test according

to instructions. Multiply result x 2.


Mix 10 ml of distilled water with 5 ml of sample water together. Run test

according to instructions. Multiply result x 3.



according to instructions . Multiply result x 5.

1 0 : 1 Dilution Factor




Mix 48 ml of distilled water with 2 ml of samp le water together. Run test



Instructions for: Acidity Method: Methyl Orange Range: 0 — 25 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions

for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.

2. Preparing the blank vial

a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be tested

b. Thoroughly wipe the vial clean 3. Preparing the reagent test vial

a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested

to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow 5 minutes for reaction

4. Performing the test

a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record

5. Press enter to continue and follow instructions on screen 6. After completion of test, discard the reagent vial according to EPA regulations

IMPORTANT & HELPFUL STEPS Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results

Rinse 5 ml syringe with distilled water when finished Be sure to allow 5 minutes for reaction in step 3e

Questions? Please call 330-437-0067 for technical assistance


Instructions for: Alkalinity

Method: Colorimetric Range: 0 — 50 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.




a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the

vial c. Recap and invert several times d. Uncap vial and carefully add exactly 5 drops of Alkalinity Reagent # 1 e. Recap and invert several times to mix f. Uncap and add 2 ml of the water to be tested to the vial g. Recap and invert several times to mix h. Thoroughly wipe the vial clean i. Allow 30 seconds for reaction




IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean

with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results

Rinse 5 ml syringe with distilled water when finished Be sure to use distilled water in step 3b Carefully add exactly 5 drops of reagent # 1 in step 3d Be sure to use exactly 2 ml of water to be tested in step 3f If water contains more than 50 mg/l, then a dilution will be necessary prior to testing

Questions? Please call 330-437-0067 for technical assistance.


Instructions for: Aluminum

Method: Aluminum (Standard Methods) Range: 0 — 2 mg/L (ppm)







to the vial c. Recap and invert 20 times d. Thoroughly wipe the vial clean e. Allow 20 minutes for reaction







Rinse 5 ml syringe with distilled water when finished Must allow 20 minutes for reaction in step 3e Fluoride may interfere with test



Instructions for: Ammonia

Method: Nesslerization (USEPA Approved) Range: 0 — 5 mg/L (ppm)

1. Press On I Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.)

2. Scroll down to desired test and press enter. This will allow the instrument to warm up while

preparing the reagent test vial, thus saving time. 3. Preparing the blank vial


b. Thoroughly wipe the vial clean

4. Preparing the reagent test vial a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested

to the vial c. Shake dropper bottle marked Ammonia Reagent # 2 and carefully add exactly 9 drops d. Thoroughly wipe the vial clean


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record





Rinse 5 ml syringe with distilled water when finished Be sure to choose Ammonia Nes when selecting test on instrument Very important to add exactly 9 drops of regent #2 If EPA approved method is required, then a distillation procedure is necessary Interferences: Calcium, Iron, Magnesium and Sulfide may precipitate with the reagent and

should be removed with Zinc Sulfate and Alkali For very low concentrations of ammonia, a distillation process may be used



Instructions for: Ammonia Salicylate

Method: Salicylate Range: 0 — 1 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.)

2. Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.





to the vial c. Recap and shake 40 times to mix d. Uncap vial and carefully add exactly 10 drops of Reagent A e. Recap and shake 40 times to mix f. Uncap vial and carefully add 2 drops of Reagent B g. Recap and shake 40 times to mix h. Thoroughly wipe the vial clean i. Allow 25 minutes for reaction







Rinse 5 ml syringe with distilled water when finished Be sure to add correct amount of drops in steps 4d and 4f Be sure to shake 40 times to mix Be sure to allow 25 minutes for reaction



Instructions for: Arsenic

Method: Silver Diethyldithiocarbamate (USEPA Approved) Range: 0 - 0.5 mg/L

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time. Preliminary Procedure (Caution!! This test contains hazardous materials. Use of fume hood is recommended. Refer to MSDS for proper handling.)

2. Transfer 40 ml of the water to be tested into the conical flask while stirring using a Hotplate I

Stirrer (It is recommended that a magnetic stir bar be used also) 3. Carefully add exactly 5 mL of Reagent A (Use caution: contains Concentrated

Hydrochloric Acid. Extremely corrosive!) 4. Carefully add exactly 2 mL of Reagent B 5. Carefully add exactly 8 drops of Reagent C 6. Allow solution to stir for 10-15 minutes 7. While the solution is stirring in step 3e, insert glass wool (provided) loosely into scrubber

assembly as shown in figure 1 8. Impregnate the wool with 8 drops of Reagent D (Be sure Reagent D does not drip out of

scrubber tube)

9. Carefully add exactly 5 mL of the indicator solution to the absorber tube (see figure 1)

Arsine generator assembly


Arsenic Reagent Instructions - Page 2 10. Carefully connect the scrubber tube to the absorber tube using the metal clamp. After solution

has stirred in step 3e, add the entire contents of capsule from the bag labeled Zinc. 11. Immediately connect the scrubber/absorber assembly to the conical flask. Be sure the

connection is firm. Vacuum grease (not included) can be used in this joint, however if the connection is made properly, this is not necessary. If the connections are made properly, there should be bubbles coming through absorber assembly. Allow 30 minutes for solution to complete reaction

12. After 30 minutes, add heat to the conical flask for 3-4 minutes to ensure the reaction is

complete. 13. Preparing the blank vial

a. Select the capped vial marked Arsenic Blank (supplied with each test and will be used for all 50 tests)


a. Uncap one of the reagent test vials b. Disconnect the absorber tube from the scrubber tube by removing the clamp c. Fill the empty vial with contents of the absorber tube d. Recap and thoroughly wipe the vial clean


a. Insert the cleaned blank vial in the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record

16. Press enter to continue and follow instructions on screen 17. After completion of test, discard the solution in the glassware and vial according to EPA

regulations IMPORTANT & HELPFUL STEPS


with a dry towel. Foreign residue of any kind or smudges from handling may affect results Rinse all glassware with distilled water when finished Be sure all connections are tight It may not be necessary to add 8 drops of Reagent D to every test that is performed. If upon

successive tests the glass wool is very moist and there are no color changes in the wool, addition of Reagent D may not be necessary.

Question? Please call 330-437-0067 for technical assistance


Instructions for: Barium

Method: Turbidimetric Range: 0 — 50 mg/L (ppm)







to the vial c. Recap and invert several times to mix d. Thoroughly wipe the vial clean e. Allow 5 minutes for reaction










Instructions for: Boron Method: Carmine Red (Standard Methods) Range: 0 — 1 5 mg/L (ppm)



a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled water b. Thoroughly wipe the vial clean

3. Preparing the reagent test vial (Caution!! This test contains Concentrated Sulfuric Acid and

Hydrochloric Acid. Refer to MSDS for proper handling.) Uncap one of the reagent test vials a. Pipette 5 ml of the Concentrated Sulfuric Acid into the vial b. Recap vial and set aside c. Rinse the pipette several times with distilled water before next step d. Pipette I ml of the water to be tested into a conical flask e. Carefully add exactly 1 drop of Concentrated Hydrochloric Acid to the flask. (Make sure

the drop hits the water or inconsistent readings may occur..) Carefully add exactly 5 ml of Concentrated Sulfuric Acid to the flask

f. Swirl solution in flask for at least 1 minute. (Make sure the solution is thoroughly mixed.) g. Allow the flask to cool to room temperature h. Shake contents in the vial in step 4a — 4c until thoroughly dissolved i. Pour contents of the vial into the flask j. Swirl solution in flask for at least 1 minute. (Make sure the solution is thoroughly mixed.) k. Allow solution in flask to set for 30 to 45 minutes for full color development l. Uncap clean empty vial and carefully fill with solution from flask m. Recap and thoroughly wipe the vial clean





IMPORTANT & HELPFUL STEPS Page 2

Use Caution when performing procedure. Consult MSDS for proper handling. Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean


Be sure to follow instructions and add exact amounts called for in procedure When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of

distilled water Questions? Please call 800-543-9283 for technical assistance


Instructions for: Bromine

Method: DPD (Standard Methods) Range: 0 - 8 mg/L (ppm)






to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow one minute for reaction






with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished Be sure to allow 1 minute for reaction in step 4e



Instructions for: Cadmium

Method: Dithizone (Standard Methods) Range: 0 — 1 mg/L (ppm)


for the Colorimeter for further instructions) Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.

2. Preparing the blank vial (Caution! ! This test contains Chloroform. Use of a fume hood is

recommended. Refer to MSDS for proper handling.) a. Select one clean empty vial (supplied with each test kit) and fill with Chloroform and

recap. b. Thoroughly wipe the vial clean

3. Preparing the reagent test vial a. Transfer 10 ml of the water to be tested into a 125 ml Separatory funnel b. Carefully add exactly 0.4 ml of Reagent A to the funnel. c. Insert the stopper and carefully shake for 10 seconds to mix d. Carefully add exactly 2 ml of Reagent B to the funnel e. Insert the stopper and carefully shake for 10 seconds to mix f. Carefully add exactly 0.4 ml of Reagent C to the funnel g. Insert the stopper and carefully shake for 10 seconds to mix h. Carefully add exactly 6 ml of Stock Dithizone solution to the funnel i. Insert the stopper and carefully shake for 1 minute to mix. Open stopper about every 15

seconds to relieve pressure j. Drain bottom layer into a 25 ml glassware and keep for additional steps k. Carefully add exactly 4 ml of Chloroform to the funnel l. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve

pressure m. Drain bottom layer into the 25 ml glassware used in step 4j n. Drain remaining solution from funnel and discard according to EPA Regulations o. Rinse funnel thoroughly with distilled water p. Pour solution from 25 ml glassware in step 4m back into funnel q. Carefully add exactly 10 ml of Reagent D to the funnel r. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve

pressure s. Drain bottom layer from funnel and discard according to EPA Regulations t. Carefully add exactly 2 ml of Chloroform to the funnel u. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve

pressure v. Drain bottom layer from funnel and discard according to EPA Regulations w. Carefully add exactly 0. 1 ml of Reagent C to the funnel x. Carefully add exactly 6 ml of Working Dithizone solution (1 0 % of Stock Solution) to the

funnel. Working Dithizone Solution must be prepared daily or before each test. (See Helpful Hints for preparation)

y. Carefully add exactly 2 ml of Reagent E to the funnel


Cadmium - Reagent Instructions — page 2

z. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve pressure

aa. Drain the bottom layer into a clean empty vial with this solution and cap. This is the vial for the test

bb. Allow I minute for reaction cc. Thoroughly wipe the vial dd. Discard remaining solution according to EPA Regulations


a. Insert the cleaned blank vial into the cuvette opening ofthe Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record

5. Press enter to continue and follow instructions on screen 6. After completion of test, discard solution from test preparation and vials according to EPA

regulations IMPORTANT & HELPFUL STEPS

(Caution!! This test contains Chloroform. Use of a fume hood is recommended. Refer to MSDS for proper handling.)



When finished, rinse all glassware with I + 1 Nitric Acid and follow with several rinses of distilled water

Fill Blank vial with Chloroform and cap Working Dithizone solution is 10 % of Stock Dithizone solution. Mix .6 ml of Stock Solution with

5.4 ml of Chloroform. Working Dithizone solution must be prepared daily or before each test. Care must be taken when draining layers


Instructions for: Calcium Hardness

Method: Eriochrome Blue Black R Range: 0 — 300 mg/L (ppm)







vial c. Recap and invert several times d. Uncap vial and carefully add exactly 5 drops of Calcium Reagent #2 e. Recap and invert 5 times to mix f. Uncap the vial and use the 1 ml syringe (supplied with box of reagents) to add 0.2 ml of

the water to be tested to the vial g. Recap and invert 5 times to mix h. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Be sure to use distilled water in step 3b Carefully add exactly 5 drops of reagent # 2 in step 3d Carefully add exactly 0.2 ml of the water to be tested In step 3f

Please call 330 437-0067 for technical assistance.


Instructions for: Carbon Dioxide







to the vial c. Recap and invert until all chemicals have dissolved. Do not shake vial d. Uncap vial and carefully add exactly 1 drop of Carbon Dioxide reagent A e. Recap and invert 5 times to mix f. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Do not shake vial in step 3c Care must be taken to add exactly one drop of reagent A in step 3d


Instructions for: Chloramine T

Method: DPD Range: 0 — 20 mg/L (ppm)


for the Colorimeter for further instructions.) 2. Scroll down to desired test and press enter. This will allow the instrument to warm up while















Instructions for: Chloride








to the vial c. Recap and shake for 5 seconds d. Uncap vial and carefully add exactly 10 drops of Chloride Reagent #2 e. Recap and invert several times to mix f. Thoroughly wipe the vial clean g. Allow 10 minutes for reaction h. Un-dissolved reagent should settle on bottom and not be disturbed when handling


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, gently insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record





Rinse 5 ml syringe with distilled water when finished Carefully add exactly 10 drops in step 3d Be sure to allow 10 minutes for reaction in step 3g Be sure to gently place the test vial into Colorimeter in step 5c so that the un-dissolved reagent

is not disturbed


Instructions for: Chlorine Free

Method: DPD (USEPA Approved) Range: 0- 4 mg/L (ppm)



preparing the reagent test vial, thus saving time.

3. Preparing the blank vial a. Select the uncapped empty vial (supplied with each test kit) and fill with

the water to be tested b. Thoroughly wipe the vial clean

4. Preparing the reagent test vial

a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the

water to be tested to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow one minute for reaction


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen

6. After completion of test, discard the reagent vial according to EPA regulations



with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished Shelf life is no longer than 6 months



Instructions for: Chlorine Total Method: DPD (USEPA Approived) Range: 0 — 4 mg/L (ppm)











IMPORTANT & ITELPFUL STEPS



Rinse 5 ml syringe with distilled water when finished Shelf life is no longer than 6 months



Instructions for: Chromate





a. Select the vial marked “Blank” (supplied with each test kit and to be used for all 50 tests)



to the vial c. Recap d. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Questions? Please call 330-437-0067 for technical assistance


Instructions for: Color

Method: APHA Platinum — Cobalt (Standard Methods) Range: 0 — 500 APHA




a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled water Thoroughly wipe the vial clean


a. Uncap one of the reagent test vials (all vials are empty) and fill with the water to be tested

b. Recap and thoroughly wipe the vial clean 5. Performing the test








Instructions for: Copper

Method: Bathocuporine (Standard Methods) Range: 0 — 3 mg/L (ppm)




3. Preparing the blank vial a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be

tested b. Thoroughly wipe the vial clean



to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen





Rinse 5 ml syringe with distilled water when finished pH of the water to be tested should be between 3 and 6



Instructions for: Cyanide Free Method: Pyridine – Pyrazalone (Standard Methods) Range: 0 — 0.3 mg/L (ppm)



preparing the reagent test vial, thus saving time. 3. Preparing the reagent test vial


to the vial. c. Uncap Test Vial (test vial #1) and add 1 level spoon of Reagent #1, cap, and invert 20

times. d. Fill an empty vial (test vial #2) with 5mL of sample to test and add 1 level scoop of

Reagent #2 to this vial. Invert 60 times and allow 2 minutes for reaction. e. Combine the contents of Test Vial #1 and Test Vial #2 into a 10 ml graduated cylinder.

Invert 20 times and allow to react for 5 minutes. Re-pour a portion of test solution back into Test Vial #1 (3/4 full).

f. Thoroughly wipe the vial clean 4. Preparing the blank vial

a. Add 5 mL of sample water to be tested into “test vial #2”. This will become the blank. b. Thoroughly wipe the vial clean







Rinse 5 ml syringe and capped vial with distilled water when finished Questions? Please call 330-437-0067 for technical assistance

Instructions for: Cyanuric Acid

Method: Turbidimetric Range: 0 — 200 mg/L (ppm)








to the vial c. Recap and invert several times to mix d. Thoroughly wipe the vial clean









Instructions for: Detergents Method: Methylene Blue Range: 0 — 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument (Please refer to General Operating Instructions for the Colorimeter for further Instructions.)



a. Select one clean empty vial (supplied with each test kit) and fill with chloroform. b. Recap and thoroughly wipe the vial clean

4. Preparing the reagent test vial (Caution!! This test contains Chloroform and Sulfuric

Acid Solution. Use of a fume hood is recommended. refer to MSDS for proper handling.)

a. Transfer 10 ml of the water to be tested into the Separatory funnel b. Carefully add exactly 1 ml of indicator solution to the funnel c. Swirl funnel to mix solution d. Carefully add exactly 7 drops of Reagent # I to the funnel e. Insert the stopper and carefully shake for 30 seconds f. Place funnel back in upright position and open stopper to relieve pressure g. Carefully add 2 drops of Reagent # 2 to the funnel h. Reinsert the stopper and shake for 20 seconds i. Place funnel back in upright position and open stopper to relieve pressure j. Carefully add exactly 2 drops of Reagent #3 k. Reinsert the stopper and shake for 20 seconds l. Place funnel back in upright position and open stopper to relieve pressure m. Carefully add exactly 1 0 ml of Chloroform to the funnel n. Reinsert the stopper and shake for 20 seconds o. Place funnel back in upright position and open stopper to relieve pressure p. Carefully add exactly 7 drops of Reagent #4 q. Reinsert the stopper and shake for 20 seconds r. Place funnel back in upright position and open stopper to relieve pressure s. Allow the layers to separate for 30 seconds t. Drain solution from the bottom layer of the funnel into a clean empty vial u. Thoroughly wipe the vial clean



6. Press enter to continue and follow instructions on screen 7. After completion of test, discard solution in the funnel and vials according to EPA regulations


Detergents Reagent Instructions Page 2 IMPORTANT & HELPFUL STEPS

Use caution when performing procedure. Consult MSDS for proper care. Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean


When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of distilled water

Be sure to add correct amounts called for in procedure Questions? Please call 330-437-0067 for technical assistance


Instructions for: Dissolved Oxygen

Method: Azide Modification (Standard Methods) Range: 0 — 10 mg/L (ppm)





a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 8 ml (4 ml twice) of the water

to be tested to the vial c. Carefully add exactly 2 drops of reagent A and 2 drops of reagent B d. Recap and invert for 1 minute to mix e. Allow 2 minutes for reaction f. Uncap vial and carefully add exactly 20 drops of Reagent C g. Recap and invert for I minute to mix h. Allow 5 minutes for reaction (If reagent is not dissolved, shake I minute and allow

another 5 minutes for reaction) i. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Be sure to add exactly 8 ml of water to be tested to the vial in step 3b Take care to add correct amount of drops in steps 3c and 3f



Instructions for: Fluoride High

Method: Spadns (USEPA Approved) Range: 0 — 6 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (P1ease refer to General Operating Instructions



a. Select one of the reagent vials and uncap b. Use the 5 ml syringe (supplied with each test kit) and add 2 ml of the 3 ppm Standard c. Recap and invert several times d. Place a mark on the cap of this vial (This blank will be used for the entire box) e. Thoroughly wipe the vial clean f. Rinse the 5 ml syringe with distilled water before next step

3. Preparing the reagent test vial (If Chlorine is present, follow Step 4)


to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Follow Step 5

4. Preparing the reagent test vial (only if Chlorine is present)

a. In a separate clean container, fill with 25 ml of the water to be tested b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml

container and mix c. Uncap one of the reagent test vials d. Use the 5 ml syringe (supplied with Colorimeter) and add 2 ml of the 25 ml solution

mixed with Sodium Arsenite to the vial e. Recap and invert several times f. Thoroughly wipe the vial clean g. Discard the remaining 23 ml Sodium Arsenite solution





Instructions for: Fluoride High Page 2 IMPORTANT & HELPFUL STEPS



Rinse 5 ml syringe with distilled water after each use The blank should only be used with the box of reagents it came from. Discard blank after each

box of reagents and prepare a new blank with each new box


Instructions for: Fluoride Low Method: Spadns (USEPA Approved) Range: 0 — 2 mg/L (ppm)


2. Preparing the blank vial a. Select one of the reagent vials and uncap b. Use the 5 ml syringe (supplied with each test kit) and add 5 ml of the 1 ppm Standard c. Recap and invert several times d. Place a mark on the cap of this vial (This blank will be used for the entire box) e. Thoroughly wipe the vial clean f. Rinse the 5 ml syringe with distilled water before next step

3. Preparing the reagent test vial (If Chlorine is present, go to Step 4) a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested

to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Follow Step 5




mixed with Sodium Arsenite to the vial e. Recap and invert several times f. Thoroughly wipe the vial clean g. Discard the remaining 20 ml Sodium Arsenite solution





Instructions for: Fluoride Low Page 2 IMPORTANT & HELPFUL STEPS




box of reagents and prepare a new blank with each new box Questions? Please call 330-437-0067 for technical assistance


Instructions for: Fluoride Low - P Method: Spadns Range: 0 — 2 mg/L (ppm)


2. Scroll down to Fluoride Low test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.

3. Preparing the blank vial a. Select one of the reagent vials and uncap b. Use the 5 ml syringe (supplied with each test kit) and add 2 ml of the 1 ppm Standard c. Recap and invert several times d. Place a mark on the cap of this vial (This blank will be used for the entire box) e. Thoroughly wipe the vial clean f. Rinse the 5 ml syringe with distilled water before next step

4. Preparing the reagent test vial (If Chlorine is present, follow Step 5) a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 2 ml of the water to be tested to the vial 3. Recap and invert several times 4. Thoroughly wipe the vial clean 5. Follow Step 6

5. Preparing the reagent test vial (Chlorine is present)


container and mix c. Uncap one of the reagent test vials d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution mixed with

Sodium Arsenite to the vial e. Recap and invert several times f. Thoroughly wipe the vial clean g. Discard the remaining 20 ml Sodium Arsenite solution


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen g. After completion of test, discard the reagent vial according to EPA regulations


Instructions for: Fluoride Low – P Page 2





box of reagents and prepare a new blank with each new box



Instructions for: Formaldehyde

Method: Chromotropic Acid Range: 0 — 100 mg/L (ppm)



a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled water b. Thoroughly wipe the vial clean

3. Preparing the reagent test vial (Caution!! This test contains Concentrated Sulfuric Acid

and is extremely corrosives Refer to MSDS for proper handling) a. Carefully add 4 ml of the Concentrated Sulfuric Acid to a 125 ml Erlenmeyer flask b. Add exactly 1 drop of Regent # 1 c. Swirl flask carefully to mix d. Immediately add exactly 4 ml of the water to be tested to the flask e. Swirl flask carefully to mix f. Allow 10 minutes for reaction g. Add 5 ml of distilled water to the flask h. Swirl flask carefully to mix i. Carefully pipette 5 ml of this solution into a clean empty vial (supplied with test) j. Thoroughly wipe the vial clean







Rinse all glassware thoroughly with distilled water Use caution when performing procedure. Consult MSDS for proper care. Be sure to add correct amounts listed in each step Be sure to allow 10 minutes for reaction in step 3f



Instructions for: Hex Chrome

Method: 1,5 Diphenylcarbohydrazide (USEPA Approved) Range: 0 — 0.7 mg/L (ppm)






to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow ten minutes for reaction





Select desired test to allow the instrument to warm up Be sure the outside of vial is thoroughly cleaned by dipping vial in water and wiping clean with

a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished Be sure to allow the required amount of time for reaction If using EZ-Check auto-shut off may occur after 12 minutes



Instructions for: Hydrazine

Method: P Dimethylaminabenzaldehyde Range: 0 — 0.5 mg/L (ppm)







to the vial c. Recap and invert 1 0 times to mix d. Thoroughly wipe the vial clean e. Allow 10 minutes for reaction






with a dry paper towel. Foreign residue of any kind or smudges from handling may, affect results

Rinse 5 ml syringe with distilled water when finished Be sure to analyze the test vial immediately to avoid auto-oxidation. To prevent, place 5 ml of

HCl (1 + 9) in a 50 ml volumetric flask and fill to 50 ml with water to be tested Use HC1 (1 + 9) for dilution instead of distilled water, if a dilution is required.



Instructions for: Iodine

Method: DPD (Standard Methods) Range: 0 — 10 mg/L (ppm)







to the vial c. Recap and invert several times to mix d. Thoroughly wipe the vial clean e. Allow one minute for reaction









Instructions for: Iron

Method: Phenanthroline (Standard Methods) Range: 0 — 4 mg/L (ppm)













with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished



Instructions for: Lead

Method: Dithizone (Standard Methods) Range: 0 — 2 mg/L (ppm)

1. Press On I Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.


a. Select one clean empty vial (supplied with each test kit) and fill with chloroform. b. Thoroughly wipe the vial clean

3. Preparing the reagent test vial (Caution!! This test contains Chloroform and

Potassium Cyanide solution. Use of a fume hood is recommended. Refer to MSDS for proper handling.)

a. Transfer 10 ml of Chloroform and 10 ml of water to be tested into the Separatory funnel

b. Carefully add exactly 0.2 ml of the dithizone solution to the funnel c. Insert the stopper and carefully shake for 10 seconds to mix d. Place funnel back in upright position and open stopper to relieve pressure e. Carefully add exactly 2 drops of Reagent # 1 to the funnel f. Insert the stopper and carefully shake for 30 seconds g. Place funnel back in upright position and open stopper to relieve pressure h. Reinsert the stopper and allow 60 seconds for reaction

i. The presence of Lead will cause the bottom half of the solution to be red or pink.

1. If the solution in the bottom of the funnel is green remove the stopper and add 1 or 2 drops of reagent Lead reagent# 2 to the separatory funnel, insert stopper and shake for 30 seconds.

ii. Allow layers to separate for 30 seconds and drain from the bottom layer of

the funnel into the empty vial and fill 3/4 full. i. Thoroughly wipe the vial clean





Instructions for: Lead Page 2 IMPORTANT & HELPFUL STEPS




Interferences: Most metals which form dithizonates are eliminated by double extraction controlled pH 8.5 — 9.0. Organic material must be removed by acid digestion.

Lead - Reagent Instructions — Page 2 Use caution when performing procedure. Consult MSDS for proper care.



Instructions for: Magnesium

Method: Titan Yellow Range: 0 — 10 mg/L (ppm)






a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 6 ml (3 ml twice) of the water

to be tested to the vial c. Recap and invert 5 times d. Uncap vial and carefully add exactly 20 drops of Magnesium Reagent A e. Recap and invert 5 times to mix f. Uncap vial and carefully add 5 drops of Magnesium Reagent B g. Recap and invert 20 times h. Thoroughly wipe the vial clean i. Allow one minute for reaction







Rinse 5 ml syringe with distilled water when finished Add 6 ml (3m1 twice) of water to be tested in step 3b Care must be taken when adding drops in steps 3d and 3f



Instructions for: Manganese High

Method: Cold Periodate Oxidation (USEPA Approved) Range: 0 — 20 mg/L (ppm)






to the vial c. Recap and invert 60 times d. Uncap vial and carefully add 1 level spoon of Manganese High Reagent # 1 e. Recap and invert 20 times to mix f. Thoroughly wipe the vial clean g. Allow one minute for reaction









Instructions for: Manganese Low

Method: Formaldoxime Range: 0 — 3 mg/L (ppm)






a. Uncap one of the reagent test vials (All vials are empty) b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested

to the vial c. Carefully add exactly 5 drops of reagent # I d. Immediately add exactly 5 drops of reagent # 2 e. Recap and invert several times to mix f. Allow (2) two minutes for reaction g. Uncap vial and carefully add exactly 5 drops of reagent #3 h. Immediately add exactly 5 drops of reagent #4 i. Recap and invert several times to mix j. Allow (10) ten minutes for reaction k. Thoroughly wipe the vial clean l. Allow one minute for reaction


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen







Instructions for: Mercury Method: Dithizone (Standard Methods) Range: 0 — 2 mg/L (ppm)


for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.


a. Select one clean empty vial (supplied with each test kit) and fill with chloroform. b. Recap and thoroughly wipe the vial clean

3. Preparing the reagent test vial (Caution!! This test contains Chloroform. Use of a fume

hood is recommended. Refer to MSDS for proper handling.) a. Transfer 10 ml of the water to be tested into the Separatory funnel b. Carefully add exactly 6 drops of Reagent A to the funnel c. Insert the stopper and carefully shake for 10 seconds to mix d. Place funnel back in upright position and open stopper to relieve pressure e. Carefully add exactly 10 ml of Chloroform to the funnel f. Carefully add exactly 0.2 ml of Dithizone Reagent to the funnel g. Insert the stopper and carefully shake for 20 seconds h. Place funnel back in upright position and open stopper to relieve pressure i. Carefully add exactly 4 drops of Reagent B to the funnel j. Reinsert the stopper and shake for 20 seconds k. Place the funnel back in upright position and open stopper to relieve pressure l. Allow approximately 30 seconds for layers to separate in the funnel

i. The bottom layer should be a shade of orange. m. Drain solution from bottom layer of the funnel into a clean empty vial n. Recap and thoroughly wipe the vial clean





Use caution when performing procedure. Consult MSDS for proper handling. Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean



Be sure to add exact amounts called for in procedure Questions? Please call 330-437-0067 for technical assistance


Instructions for: Molybdenum Method: Mercaptoacetic Acid Range: 0-35 mg/L (ppm)







to the vial c. Recap and invert several times until powder is dissolved d. Uncap vial and carefully add exactly one drop of Molybdenum reagent # 1 e. Recap and invert 10 times to mix f. Thoroughly wipe the vial clean g. Allow one minute for reaction


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen g. After completion of test, discard the reagent vial according to EPA regulations




Rinse 5 ml syringe with distilled water when finished Powder must be dissolved in step 4c Care must be taken to add only one drop in step 4d Allow one minute for reaction in step 4g If water contains more than 35 mg/i of Molybdenum, than a dilution must be performed before

Step 4



Instructions for: Nickel

Method: Dimethyglyoxime Range: 0 — 10 mg/L (ppm) 1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions

for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.





to the vial c. Carefully add exactly 3 drops of Nickel reagent #2 d. Recap and invert 5 times e. Allow 3 — 5 minutes for reaction f. Uncap vial and carefully add exactly 4 drops of Nickel reagent # 3 g. Recap and invert 5 times to mix h. Allow between 20 — 25 minutes for reaction i. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Take extra care when adding stated amount of drops from dropper bottles Allow required reaction times



Instructions for: Nitrate High

Method: Cadmium Reduction (Standard Methods) Range: 0 — 100 mg/L (ppm)






vial c. Carefully add exactly 0.2 ml of the water to be tested to the vial d. Recap and invert several times e. Uncap vial and carefully add approximately 12 grains from the Cadmium capsule

(amount of grains should approximately be the diameter of an eraser floating in the vial) f. Recap and shake for 5 — 7 minutes to mix g. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Be sure to add 5 ml of distilled water in step 3b When grains are added in step 3d, color should be pink. If too many grains are added, color will

turn grayish purple Questions? Please call 330-437-0067 for technical assistance


Instructions for: Nitrate High








vial c. Carefully add exactly 0.2 ml of the water to be tested to the vial d. Recap and invert several times e. Uncap vial and carefully add approximately 12 grains from the Cadmium capsule

(amount of grains should approximately be the diameter of an eraser floating in the vial) f. Recap and shake for 5 — 7 minutes to mix g. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Be sure to add 5 ml of distilled water in step 3b When grains are added in step 3d, color should be pink. If too many grains are added, color will



Instructions for: Nitrate Low







to the vial c. Recap and invert several times d. Uncap vial and carefully add a few crystals of Reagent #1 (amount of grains should

approximately be the diameter of an eraser floating in the vial) e. Recap and shake for 5 — 7 minutes to mix f. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished When grains are added in step 3d, color should be pink. If too many grains are added, color will



Instructions for: Nitrite

Method: Diazotization (Standard Methods) Range: 0 — 0.4 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.





to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean









Instructions for: Orthophosphate High

Method: Vanadomolybdophosphoric (Standard Methods) Acid Range: 0 — 50 mg/L (ppm)







to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow 1 0 minutes for reaction


a. Insert the cleaned blank vial into the curvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into curvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen





Rinse 5 ml syringe with distilled water when finished Be sure to allow 10 minutes in Step 4e



Instructions for: Orthophosphate Low

Method: Stannous Chloride (Standard Methods) Range: 0 — 2 mg/L (ppm)






to the vial c. Recap and invert 1 0 times d. Uncap vial and carefully add exactly 1 drop of Orthophosphate Low reagent # 1 e. Recap and invert 10 times to mix f. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Shelf life is no longer than 6 months Positive interference may be caused by Silica and Arsenic if sample is heated Negative interference may be caused by Arsenate, Fluoride, Sulfide, Thiosulfate and

Thiocyanate Questions? Please call 330-437-0067 for technical assistance


Instructions for: Ozone

Method: DPD Range: 0 — 5 mg/L (ppm)

















Instructions for: pH Method: Phenol Red (Standard Methods) Range: 6.4 — 8.3



3. Preparing the blank vial a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be tested b. Thoroughly wipe the vial clean

4. Preparing the reagent test vial (If Chlorine is present, follow Step 5) a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean

5. Preparing the reagent test vial (Chlorine is present) a. In a separate clean container, fill with 25 ml of the water to be tested b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml container and mix c. Uncap one of the reagent test vials d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution mixed with Sodium Arsenite to the vial e. Recap and invert several times f. Thoroughly wipe the vial clean g. Discard the remaining 20 ml Sodium Arsenite solution

6. Performing the test a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted,. insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record f. Press enter to continue and follow instructions on screen



Continued

Instructions for: pH Method: Phenol Red Range: 6.4 — 8.3


Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results

Rinse 5 ml syringe with distilled water when finished



Instructions for: pH Method: Methyl Blue (Standard Method) Range: 7.6 — 9.6





b. Thoroughly wipe the vial clean . 3. Preparing the reagent test vial (If Chlorine is present, follow Step 4)


to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Go to Step 5




mixed with Sodium Arsenite to the vial e. Recap and invert several times f. Thoroughly wipe the vial clean S g. Discard the remaining 20 ml Sodium Arsenite solution


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening’ d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record



Instructions for: pH Page 2 IMPORTANT & HELPFUL STEPS



Rinse 5 nil syringe with distilled water when finished Questions? Please call 330-437-0067 for technical assistance


Instructions for: Phenol

Method: Anünoantipyrine (USEPA Approved) Range: 0 — 5 mg/L (ppm)






to the vial c. Recap and invert several times being sure reagent is completely dissolved d. Thoroughly wipe the vial clean

4. Performing the test .








Instructions for: Phosphate Total Method: Vanadomolybdophosphoric Acid (USEPA Approved) Range: 0 — 50 mg/L (ppm)


2. Preliminary Procedure

a. Place 50 ml of the water to be tested into an appropriately size glassware b. Carefully add exactly 1 ml of 1 + 1 Normal Sulfuric Acid c. Add the contents of 1 capsule labeled Ammonium Persulfate d. Using a Hotplate I Stirrer slowly bring to a boil until a volume of 10 ml is left e. Remove solution from heat when 10 ml is reached f. While stirring, use a pH meter to adjust the pH of the 10 ml solution to 7.00 by adding I

drop of 1 Normal Sodium Hydroxide. Continue to add 1 drop at a time until pH of 7.00 g. After solution cools down, bring back to 50 ml by diluting with distilled water h. Filter solution if it is not clear




a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the solution from step

3 above to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow 10 minutes for reaction





Instructions for: Phosphate Total Page 2 IMPORTANT & HELPFUL STEPS


with a dry paper towel. Foreign residue of any kind or Smudges from handling may affect results

Rinse all glassware and 5 ml syringe with distilled water when finished Positive interference is caused by Silica and Arsenic if heated Negative interference is caused by Arsenate, Fluoride, Sulfide, Thiosulfate and Thiocyanate



Instructions for: Potassium

Method: Tetraphenylboron Range: 0 — 1O mg/L (ppm)







to the vial c. Recap and invert 20 times to mix d. Uncap vial and carefully add exactly 3 drops of Reagent A e. Recap and invert 20 times to mix f. Thoroughly wipe the vial clean g. Allow one minute for reaction


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening (Invert

reagent test vial 1 time immediately before inserting into cuvette) d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record





Rinse 5 ml syringe with distilled water when finished Carefully add 3 drops of Reagent A in step 3d Invert reagent test vial 1 time immediately before inserting into cuvette in step 4c



Instructions for: Silica High Method: Colorimetric (Standard Methods) Range: 0 — 100 mg/L (ppm)







to the vial c. Carefully add exactly 2 drops of Reagent # 1 (Shake before using) d. Recap and invert 5 times e. Uncap vial and carefully add exactly 4 drops of Reagent # 2 (Shake before using) f. Recap and invert 20 times to mix g. Allow 5 minutes for reaction h. Uncap vial and carefully add exactly 2 drops of Reagent #3 (Shake before using) i. Recap and invert 20 times to mix j. Thoroughly wipe the vial clean k. Allow 2 minutes for reaction







Rinse 5 ml syringe with distilled water when finished Carefully add correct number of drops in steps 3c, 3e and 3h Be sure to allow proper amount of time for reaction in steps 3g and 3k



Instructions for: Silica Low

Method: Colorimetric (Standard Methods) Range: 0 — 10 mg/L (ppm)







to the vial c. Uncap vial and carefully add 1 spoonful (5 mg spoon) from bottle labeled Reagent # 1 d. Recap and invert 5 times e. Allow 60 seconds for reaction f. Uncap vial and carefully add 1 spoonful (5 mg spoon) from bottle labeled Reagent # 2 g. Recap and invert 60 times to mix h. Thoroughly wipe the vial clean i. Allow 10 minutes for reaction







Rinse 5 ml syringe with distilled water when finished Allow proper amount of times for reaction in steps 3e and 3i



Instructions for: Silver Method: Dithizone (Standard Methods) Range: 0 — 5 mg/L (ppm)






a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of Acetone (supplied) to

the vial c. Recap and invert 20 times to mix d. Rinse 5 ml syringe with distilled water before next step e. Uncap vial and using 5 ml syringe, add 4 ml of the water to be tested to the vial f. Recap and invert 20 times to mix g. Uncap vial and carefully add exactly 3 drops of Silver reagent #2 h. Recap and invert 20 times to mix i. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Be sure to add 5 ml in step 3b and 4 ml in step 3e Carefully add exactly 3 drops of Silver reagent #2



Instructions for: Sulfate Method: Turbidimetric (Standard Methods) Range: 0 — 300 mg/L (ppm);




3. Preparing the blank vial a. Select the uncapped empty vial (supplied with each test kit) and fill with the water

to be tested b. Thoroughly wipe the vial clean


a. Uncap one of the reagent test vials

b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean



6. After completion softest, discard the reagent vial according to EPA regulations


Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping

clean with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical


Instructions for: Sulfide

Method: Methylene Blue (USEPA Approved) Range: 0 — 1.8 mg/L (ppm)




a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be tested•



to the vial c. Carefully add exactly I drop of Sulfide reagent # 2 d. Recap and invert 5 times e. Allow 2 minutes for reaction f. Uncap vial and carefully add exactly 3 drops of Sulfide reagent # 3 g. Recap and invert 5 times to mix h. Thoroughly wipe the vial clean







Rinse 5 ml syringe with distilled water when finished Care must be taken to add correct number of drops in steps 3c and 3f



Instructions for: Tannin & Lignin

Method: Colorimetric (Standard Methods) Range: 0 — 7 mg/L (ppm) 1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions






to the vial c. Recap and invert 1 0 times to mix d. Allow 1 minute for reaction e. Uncap vial and carefully add exactly 20 drops of Reagent B f. Recap and invert 10 times to mix g. Thoroughly wipe the vial clean h. Allow 5 minutes for reaction







Rinse 5 ml syringe with distilled water when finished Be sure to add correct amount of drops in step 3e Interferences: Such reducing substances as 2 mg/LFerrous Iron and Sodium Sulfite individually

produce a color equivalent to 1 mg/L Tannic Acid. Sulfite Interferences can be removed by adding 1 ml of Formaldehyde Solution to the water.



Instructions for: Total Chrome

Method: 1,5 Diphenylcarbohydrazide (Standard Methods) Range: 0 — 0.7 mg/L (ppm)

1. Preliminary Sample Treatment a. Place 50 ml of water to be tested into appropriately sized glassware b. Add 5 ml of Total Chrome reagent A (Use caution: contains 50 % Sulfuric Acid) c. Add 1 ml of Total Chrome reagent B d. Allow to sit 1 0 minutes for reaction e. Using a Hotplate I Stirrer, boil sample until colorless f. Allow to cool and then add distilled water to bring back to 50 ml or more g. Bring solution to boil again and add drops from Total Chrome reagent C dropper bottle

until a faint pink color persists for 10 minutes while boiling h. After pink color persists for 10 minutes of boiling, add drops from Total Chrome reagent

D dropper bottle until sample becomes colorless i. Boil for an additional 2 minutes to remove any excess Azides j. After sample cools down bring back to 50 ml by diluting with distilled water


for the Colorimeter for further instructions.) Scroll down to Hex Chrome test and press enter. This will allow the instrument to warm up while preparing the reagent test vial, thus saving time.




a. Uncap one of the reagent test vials b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the solution from Step

10 above to the vial c. Recap and invert several times d. Thoroughly wipe the vial clean e. Allow ten minutes for reaction


a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter b. Place the black cover over the vial and press enter c. When prompted, insert the cleaned reagent test vial into cuvette opening d. Place the black cover over the vial and press enter e. Read the results displayed on the screen and record as Total Chrome



Instructions for: Total Chrome Page 2 IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up Be sure the outside of vial is thoroughly cleaned by dipping vial in water and wiping clean with

a dry paper towel. Foreign residue of any kind or smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished Be sure to allow the required amount of time for reaction Pink color in Step 1-g should remain after 10 minutes of boiling Answer is to be recorded as Total Chrome (will display as Hex Chrome) Use caution when performing procedure. Consult MSDS for proper care.



Instructions for: Total Hardness

Method: EDTA Range: 0 — 400 mg/L (ppm)







vial c. Recap and invert several times d. Uncap vial and using the 1 ml Syringe (supplied with Colorimeter) carefully add exactly

0. 1 ml of the water to be tested to the vial e. Recap and invert 5 times to mix f. Thoroughly wipe the vial clean . g. Allow 30 seconds for reaction







Rinse 1 ml syringe with distilled water when finished Must use distilled water in Step 3b Be sure to add exactly 0. 1 ml of water to be tested in Step 3d



Instructions for: Turbidity Method: EPA I 80. 1 (USEPA Approved) Range: 0 — I 1TU, 0 - 1 00 NTU, 0 — 800 NTU

1. Before performing test, choose the desired range and standard to be tested.

Range 0 – 1 then use 0.5 NTU Standard Range 0 - 100 then use 50 NTU Standard

Range 0 - 1000 then use 200 NTU Standard 2. Mark two clean empty vials using a fine point permanent marker on the vial glass that is

exposed (when inserted into cuvette opening) slightly above the keypad. This will allow the user to place the vial in the proper alignment each time in the cuvette opening of the Colorimeter.

3. Fill one marked empty vial with the proper NTU Standard and thorough ly wipe clean.

4. Choose the correct turbidity test on the Colorimeter and press enter. Please refer to General

Operating instructions for the Colorimeter for further information.) 5. Insert the filled standard vial into the Colorimeter taking special care to align the mark on the

vial with the mark on the keypad next to the cuvette opening, place cover over vial and press enter.

Important Note: If performing the 0 — 1 range, then you must reuse the same vial the standard is in for the water to be tested. Follow Step 6 to 8. If performing 0 — 100 or 0 — 1000 range, then the standard vial may be kept for reuse with other Turbidity tests. Follow Step 9 and 10.

6. Pour standard solution back into its original container for later use. Rinse the now empty vial

three or more times with distilled water. 7. Fill the cleaned vial with water to be tested, thoroughly wipe clean, insert into Colorimeter

taking special care align mark on the vial with mark on the keypad, place cover over vial and press enter.

8. Read results on screen and record. Press enter to continue. 9. Fill second marked empty vial with the water to be tested, thoroughly wipe clean, insert into

Colorimeter taking special care to align the mark on the vial with the mark on the keypad, place cover over the vial and press enter.

10. Read results displayed on screen and record. Press enter to continue.

IMPORTANT STEPS

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean with a dry paper towel. Foreign residue of any kind or smudges from handling may affect results.

Take special care to properly align marks on vial and keypad. 0 — 1 range must use the same vial for standard and water to be tested a When finished, rinse vials with distilled water thoroughly for reuse



Instructions for: Zinc

Method: Zincon (USEPA Approved) Range: 0 — 5 mg/L (ppm)






to the vial c. Recap and invert several times d. Uncap vial and carefully add exactly 5 drops of Zinc Reagent A e. Recap and invert 5 times to mix f. Thoroughly wipe the vial clean g. Allow one minute for reaction





Select desired test to allow the instrument to warm up Be sure the outside of the vial is thoroughly cleaned by dipping in water and wiping clean with a

dry paper towel. Foreign residue of any kind or Smudges from handling may affect results Rinse 5 ml syringe with distilled water when finished Carefully add exactly 5 drops of Reagent A
