COLOR ALERT

A Carbon Monoxide

detecting paint

Nicole Yu

CARBON MONOXIDE POISONING

leading cause of poisoning death odorless, tasteless, colorless =

extremely hard to detect high concentration are fatal within

minutes impair the blood’s ability to transport

oxygen suffocation

CURRENT TECHNOLOGY

carbon monoxide detectors are somewhat effective

must be installed at certain locations in order to remain accurate

cannot be exposed to sunlight, humidity, and other conditions

false positives

COLOR ALERT

Requires: E. Coli bacteria amplifier gate cutM-cutS-cutL gene cutM-cutS-cutL gfp reporter gene ampicillin resistance gene KODE technology paint

Plasmid inserted

into bacteria

Ampicillin

resistance gene

carbon monoxide activator

for amplifier

gate

cutM-cutS-cutL gene

cutM-cutS-

cutL gfp =reporter gene

HOW IT WORKS

plasmid inserted into E. Coli bacteria grown on Agar plates with ampicillin and Luria broth

eliminate any bacteria that has not taken in the plasmid CO will activate the amplifier gate which will activate the

transcription factor activate the open reading frame of cutM-cutS-cutL gene

produce carbon monoxide dehydrogenase = enzyme that catalyzes conversion from CO to CO2

CO + H2O --> CO2 + 2 H+ + 2e (Carbon Cycle)

activation of cutM-cutS-cutL -- cutM-cutS-cutL gfp GLOW GREEN!!

KODE TECHNOLOGY

contains amphipathic function-spacer-lipid constructs hydrophilic functional head hydrophilic spacer lipid tail

KODE TECHNOLOGY CONT. modify a surface through process known as koding lipid tail acts like an anchor

spontaneously integrates into the phospholipid bilayer of the cell membrane

functional head is interchangeable depending on what function something that can attach to material of the wall

being painted cells secured to the wall https://www.youtube.com/watch?t=53&v=U14EnuNyJZc

EXPECTED RESULTSCarbon

MonoxideCarbon

Monoxide Dehydrogenase

Green Flouresence

0 

0 0

1 

1 1

https://scratch.mit.edu/projects/71362336/#player

WHY COLOR ALERT?

can detect both the presence and origin of CO

beneficial to those with hearing impairments who cannot hear the traditional smoke alarms

simple incorporation into house = more accuracy, less mistakes from misplacement or sensitivity

FLAWS & RISKS OF THIS DESIGN uncertain how long bacteria can survive in

such environment system may become extremely impractical

if bacteria does not have a prolonged life span

how effective FSL constructs are over multiple generation

cannot be seen when asleep in laboratory, precautions must be taken to

prevent bacteria from escaping if bacteria gets into environment, could

evolve and horizontal gene transfer could occur with other bacteria

TESTING & POTENTIAL ensure the efficacy of the device indicate possible changes that could be

implemented to improve the system if effective, design could be applied to

other areas such as cars or mines could also lead to the development of

organisms to replace other alarm systems

SOURCES Anand, Ashima, T. Satyanarayana, and & Satyanarayana: Applicability Of Co Dehydrogenase. "Applicability

of Carboxydotrophic Bacterial Carbon Monoxide Dehydrogenase(CODH) in Carbon Sequestration and Bioenergy Generation." Journal of Scientific & Industrial Research (2012): 381-84. NISCAIR Online Periodicals Repository. 16 Apr. 2012niscair online periodicals repository. Web. 29 July 2015. <http://nopr.niscair.res.in/bitstream/123456789/14154/1/JSIR%2071%286%29%20381-384.pdf>.

"Biosurface Innovation Has Never Been Easier." KODE Biotech. KODE Biotech, 2012. Web. 29 July 2015. <http://www.kodebiotech.com/>.

"CO Dehydrogenase/Acetyl-CoA Synthase (CODH/ACS)." Chemwiki. UC Davis ChemWiki, n.d. Web. 29 July 2015. <http://chemwiki.ucdavis.edu/Wikitexts/UC_Davis/UCD_Chem_124A%3A_Berben/CO_dehydrogenase/CO_dehydrogenase_2>.

"Function-spacer-lipid Construct." Wikipedia. Wikimedia Foundation, 1 Jan. 2015. Web. 29 July 2015. <https://en.wikipedia.org/wiki/Function-spacer-lipid_construct>.

Kang, Beom S., and Young M. Kim. "Cloning and Molecular Characterization of the Genes for Carbon Monoxide Dehydrogenase and Localization of Molybdopterin, Flavin Adenine Dinucleotide, and Iron-Sulfur Centers in the Enzyme of Hydrogenophaga Pseudoflava." Journal of Bacteriology. American Society for Microbiology, Sept. 1999. Web. 29 July 2015. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94076/>.

"Kodecyte.com." Kodecyte.com. N.p., n.d. Web. 29 July 2015. <http://www.kodecyte.com/>. K.R. Tremblay, Jr. "Preventing Carbon Monoxide Problems." Preventing Carbon Monoxide Problems. Colorado

State University, 3 Mar. 2015. Web. 29 July 2015. <http://www.ext.colostate.edu/pubs/consumer/09939.html>.

"MSHA - Carbon Monoxide." United States Department of Labor. U.S Department of Labor, n.d. Web. 29 July 2015. <http://www.msha.gov/Illness_Prevention/healthtopics/carbonmonoxide.htm>.

IMAGE LINKS

http://www.fleetfarm.com/products/images/medium/0000000014635.jpg http://www.nfpa.org/~/media/images/research-and-reports/report-graphic

s/nonfirecarbonmonoxide.jpg?as=1&iar=1&la=en

http://brokelyn.com/wp-content/uploads/2012/09/carbon-monoxide-detector.gif

http://media.cleveland.com/insideout_impact/photo/first-alert-detectjpg-e6b0f063e76062e5.jpg

https://upload.wikimedia.org/wikipedia/commons/thumb/9/9f/Structural_analogy_of_a_sunflower_to_space_filling_models_of_selected_FSL_constructs.png/300px-Structural_analogy_of_a_sunflower_to_space_filling_models_of_selected_FSL_constructs.png

https://upload.wikimedia.org/wikipedia/commons/thumb/8/84/Koded_biomembrane.svg/2000px-Koded_biomembrane.svg.png

http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/Articleimage/2011/MT/c1mt00042j/c1mt00042j-f4.gif