CLINICAL MICROSCOPE

BX41/BX45/BX45A/BX51BX2 SERIES

More contrast, more clarity

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Already renowned for their advanced ergonomic design, BX2 series clinical microscopes now incorporateOlympus' new UIS2 optical system to deliver a wealth of all-round performance improvements. With Plan objectives as standard equipment, they deliver flat, even, high-contrast observation images rightup to the periphery of the field of view, providing a completely new standard of visibility in routine work. Offering high quality and excellent cost performance, BX2 series microscopes make routine tasks quicker,easier, and much more efficient.

The new UIS2 optical system: quicker, easier, more efficient performancefor all routine applications.

BX45Ergonomic Laboratory Microscope

BX41Laboratory Microscope

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BX51System Microscope

BX45AAutomated Ergonomic Laboratory

Microscope

Plan objectives for image claritySetting the standard for clinical microscopes ultimately depends onimage clarity. Olympus' choice of only Plan objectives ensuresbright, clear images with excellent flatness and compensation forchromatic aberration.

Improved viewing contrastThe combination of UIS2 objectives and UIS2 eyepieces (WHNseries) with newly developed anti-reflective coatings gives higherimage contrast and a striking improvement in overall visibility.Stained specimens show greater clarity with a whiter background.

Ideal color temperature for digital imagingUIS2 optics' color temperature rendition at both the eyepiece andon the CCD is very close to natural daylight (5500K). Colorreproduction on the monitor matches more closely to what is seenduring observation.

Advanced, high-performance UIS2 optical systemOlympus' principal reason for replacing the popular and well-regarded UIS optical system with its UIS2 successor is to meet amore diverse and demanding range of future needs. Olympus'infinity-corrected optical system has the tube lens inside theobservation tube, which prevents image deterioration even if otheroptical components are added. In addition, UIS2 flexibility providesgreater freedom in system structure,even allowing equipment to be added intwo tiers simultaneously.

Environmentally-friendly UIS2 opticsRecognizing the interdependence of optical excellence and care forthe environment, Olympus makes all UIS2 objectives and WHNeyepieces from lead-free eco-glass.

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New UIS2 optics deliver specific, immediate benefits — better operability and better basic performance.

Mirror

ObjectiveInfinity space Tube

lens

Parallel flux

Image forming

ray Primary imageforming plane

Image is formed at a single point

■ Infinity-corrected optical light pathInfinity-corrected optics incorporates a parallel luminous fluxbetween the objective and tube lens. This is known as theinfinity space. Even if a mirror is inserted into the infinity space,there is no image distortion and no deterioration in imagequality.

Conventional image

UIS2 image

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Durable stage with no protrusions The BX2 series employs a rackless stage in which the X-axis guidedoes not protrude. This means there is nothing for the operator'shand to accidentally strike when replacing a specimen or adjustingfocus. The stage is ceramic coated tomaximize durability and ensureconsistently smooth specimenmovement.

Fatigue-free operation with minimum hand movementsThe light intensity adjustment controls is located near the front ofthe microscope. Users can keep theirarms on the table and performoperations with minimal handmovements. The BX45A is equippedwith a hand set to switch objectives.

Easy specimen movementThe XY handle of the stage is fittedwith a rubber cap for easy, fingertipoperation.

Tilting observation tubes allow better ergonomic operationThe BX2 series tilting observation tubes (U-CTBI, U-TBI-3, U-TTBIand U-ETBI) are designed to promote comfort and reduce fatigue,enabling operators to maintain concentration for long periods. Onemodel emphasizes high cost efficiency,another offers improved ergonomicsderived from detailed research intoobservation posture, and anotherpresents the observation image movingin the same direction as the specimen.Users can therefore make their choicesbased on purpose and preference.

Superior cost-efficiency and ergonomics for high-throughputclinical microscopy.

U-TTBI U-ETBI

U-TBI-3

U-CTBI

U-CTBI U-TBI-3 U-ETBI U-TTBIF.N. 18 22 22 22Inclination angle 30°- 60° 5°- 35° 0°- 25° 0°- 25°Image direction Inverted Inverted Erect InvertedTelescopic No No Yes Yes

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Comprehensive range of routine work functionswith outstanding cost-performance.

Compact and rigid microscopebodyThe microscope body's compact Y-shape design, only 328mm deep,makes for easy setup even inrestricted spaces. Also the body ismade from aluminum alloy to ensurethe high rigidity needed for consistentperformance and long-term durability.

Front-positioned intensityadjustment knobThe light intensity control is mountednear the front of the unit. Users canwork with minimum hand movements,keeping arms on the table.

Detachable revolving nosepieceThe detachable revolving nosepieceallows for quick change of objectivesand makes lens cleaning easier.Quintuple to septuple versions areavailable.

Fully portableA handle on the back of themicroscope body makes it fullyportable.

Filter cassette providedThe convenient U-FC filter cassetteenables easy access to up to threefilters (ø45mm and under 2.8mmthickness).

Fine movement adjustmentThe removable fine focus handle can be attached on either side,depending on left or right hand stage configuration.

328m

m

283mm

270°

Easy-to-operate stageA rackless stage with no protrusion ofthe X-axis guide is standard for all BX2microscopes. All fine movements, aswell as stopping, are extremelysmooth. The stage can be rotated,which is useful when framing animage.

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Laboratory Microscope

BX41Laboratory Microscope

BX418-position condenser for multipleillumination methodsThe optional 8-position universalcondenser gives even more freedomto combine observation methods.Optical component combinations canaccommodate brightfield, darkfield,phase contrast, Nomarski DIC andsimple polarization observations.

1.25X

2X

4X

10X

20X

40X

100X

Pulmonary adenocarcinoma

Observation from 1.25X to 100X without changingcondensers*The optional septuple revolving nosepiece U-D7RE allows the attachment of seven separate objectives. For consecutiveobservations from 1.25X to 100X, this nosepiece can be combined with the U-SC3 swing-out condenser.*For optimal digital imaging with the 1.25X objective, please use the ultra low condenser U-ULC-2.

Digital cameras: quality with cost-performanceA full lineup of digital cameras is available, giving users a widechoice to meet their needs. The lineup includes a stand-alone typemicroscope digital camera for use without a PC; the DP70, which is suitable for all-around use from brightfield tofluorescence; and adapters are available to use Olympus consumerdigital cameras. BX41

High-contrast, high-resolution imagingHigh contrast phase imaging allows close observation of the cellinterior and of live bacteria. Using UPLFLN-PH or PLN-PH seriesobjectives, phase contrast observation from 10X up to 100X ispossible. With the U-PCD2 condenser, users can view specimens

in brightfield ordarkfield dependingon their chosencombination of opticalcomponents.

High-resolution view of double refraction structure in cellsTooth, bone, muscle tissue, nerve tissue, actomyosin fiber andmitotic spindle can all be observed, without staining. There areintermediate attachments (U-OPA/U-CPA) for orthoscopic andorthoscopic/conoscopic viewing. Various compensators make itpossible to observe awide range ofretardation. Alsoavailable is acondenser exclusivelyfor polarizationobservation, revolvingnosepiece, rotatingstage, objectives andsimple polarizingattachment.

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Equally suitable for multiple observation methods.

1U-CPA 2U-P4RE 3U-AN360P 4U-OPA 5U-POC-2

1

2

34

5

Gout inspectionBrightfield (left), Simple polarized light (right)

Brightfield observation

U-DCD U-DCW

Darkfield observation Phase contrast observation

Polarized light observation

Brighter images, with superb resolution/flatness at allmagnificationsPLAPON objectives with wide F.N. combined with the Achromatic-aplanatic condenser U-AAC will deliver excellent resolution and flatnessfrom low to highmagnifications. A dedicated ultra lowmagnification condenser(U-ULC-2) should beused for 1.25X and 2X.

Excellent darkfield effect from low to high magnificationsChoose from the 10X —100X dry darkfield condenser (U-DCD) orthe 20X —100X oil immersion darkfield condenser (U-DCW). * Please consult your nearest Olympus dealer for applicable objectives.

Small intestine

Bone marrow

U-PCD2

U-ULC-2

U-AAC

U-SC3 U-AC2

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Breast cancer Brightfield observation Papanicolaou stain Brightfield observation

Gout inspection Simple polarized light observation

AmyloidBrightfield observation(above), polarized lightobservation(below)

BX41System Microscope

BX41

9

Low-position stage makes changing specimens easyUse of a new illumination system allows the stage to be fixed at only128mm above the desk surface, the lowest position in this class ofmicroscope. Focusing is performed by up/down movement of thenosepiece, so there is no variation in stage height and all specimenscan be changed with the minimal hand movement. Focusing andstage operations can be done without removing your hands from thedesktop, significantly reducing fatigue.

Specimen observation with low contrastThe condenser’s aperture diaphragm can be shifted to give obliqueillumination. Observation of a thick transparent specimen thusbecomes easier to view due to the addition of shadow effect.

Compatible with multiple applicationsThe 3-position universal condenserallows brightfield, darkfield, phasecontrast, and simple polarizingobservations. Optical components(PH1, PH2, PH3, DFA) and filters (ø32 ND, LBD, IF550) can be easilyattached and inserted into thecondenser turret.

Swing-out condenser for observation overa wide area provides observation at low(1.25X) magnificationThe built-in swing-out condenser allows useof ultra low objectives such as 1.25X and2X. The initial observation covers a widearea, allowing the operator to quicklydetermine any zone needing closer attention.

4X to 100X observations with top lensConsecutive observation from 4X to 100X is now possible with atop lens making it no longer necessary to swing out of the opticalpath.

Convenient range of ergonomic observation tubesIn addition to the general ergonomic observation tube for invertedimage observation, there is another version for erect imageobservation (where the direction of specimen movement matchesthe direction of observation image movement).

1.25X

Ergonomic design for improved operabilityand less fatigue.

90 degrees 128mm

0–45mm

0 –25 degrees

The observation angle adjuststo the individual, reducing thestress on the operator'sshoulders and neck

Super low stage forspecimen change withminimal effort

The ergonomic design helps to maintain anupright, comfortable posture with theoperator's spine correctly aligned — the mostbeneficial position for long observation periods

Extendable binocular observation tube for an ergonomically ideal posture and comfortable observation

Numerous objectives to suit various specific purposeThe extensive range of objectives includes the PLN-PH series, forphase contrast observation, as well as no-cover objectives whichare ideal for observing smear specimens (e.g. blood) withoutrequiring a cover glass.

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U-GAN gout analyzer q U-SRG2 rotatable graduated stage w BX45-PO polarizer e U-GAN gout analyzer

q

w

e

Easy gout inspectionGout testing is simple with the BX45, as the analyzer is easilyinserted or removed from its position in the nosepiece. In addition arotating stage can be employed.

Sodium urate crystal

A key priority: reducing fatigue in cytological examinations

Motorized objective changeThrough a handswitch, the motorized operation ofchanging between 10X and 40Xobjectives is quick and easy. Thehandswitch is convenient andallows the operator to keep theirhands on the desktop.

Focusing position correction ringThe BX45A is equipped with correction rings to parfocalize theobjectives. No focus change is required when changingmagnification.

Papanicolaou stain

No need for light adjustment when changing objectivesThe 10X objective equipped with ND filter enables the samelevel of brightness even if the magnification is changed from 10X to 40X. No brightness adjustment (e.g. of light intensity) is required.

Automated Ergonomic Laboratory Microscope

BX45AAutomated

Ergonomic Laboratory Microscope

BX45A

Ergonomic Laboratory Microscope

BX45Ergonomic Laboratory Microscope

BX45

40X10X

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Outstanding optical performance and flexibleexpandability, from clinical to research applications.

Separate arm improves system performanceThe BX51 employs an entirely new structure, whereby thetransmitted light arm and the microscope frame are separated. Thisgives users greater freedom to build system configurations of theirchoice.

Observation from 1.25X to 100X without changingcondensers*The septuple revolving nosepiece U-D7RE allows the attachment ofseven separate objectives. For continuous observations from 1.25Xto 100X, this nosepiece can be combined with the U-SC3 swing-out condenser (Also compatible with the BX41.).*For optimal digital imaging with the 1.25X objective, please use the ultra low condenser U-ULC-2.

Up to 4 filters can be built inThe BX51 is equipped with three filtersin the base (ND6, ND25, LBD) and afourth can be added as an option. The levers at the side of the basemake inserting and removing filtersfrom light path easy.

DP70/Microscope digital camera & DP-BSW/SoftwareHigh-speed capture of high resolution images equivalent to 12.5million pixels. Clear, crisp fluorescent images can be obtained withhigh sensitivity and reduced noise (equivalent to ISO100-1600). The user-friendly GUI (Graphical User Interface) makes it quick andeasy to capture, adjust and store digital images.

DP70

Luminous mirror unit indicator foreasy confirmation in dark roomBright, easy-to-see self-illuminated labelsare used to denote fluorescence filter sets,easily visible in a dark room. Three filterpositions are displayed simultaneouslymaking selection of the next filter easy andintuitive.

Tuberculosis bacteria

BX-URA2BX-RFA

Fluorescence observation

New improvements in Olympus fluorescence observationTwo illuminators are offered: the arm-integrated, high-rigidityreflected light illuminator BX-URA2 and the fluorescenceilluminator BX-RFA. Each can be fitted with six fluorescencemirror units which can be easily interchanged with the turretrotation. Their efficient illumination achieves twice the brightnessof conventional fluorescence images.

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Newt testis

Nomarski DIC observation

q

rt w

e

Image optimization according to specimen characteristicsTo ensure the optimum observation image for differentspecimens, the BX51/BX41 have DIC sliders with different levelsof shearing. Choose from several types: the U-DICT or U-DICTS,which offer high all-round performance; the U-DICTHC for highcontrast imaging of thin specimens; or the U-DICTHR for highresolution, glare-free observation of thick specimens. An 8-position universal condenser (optional) can be combined withother optical components for conducting a variety of brightfield,darkfield, phase contrast, Nomarski DIC and simple polarizingobservations.

q U-UCD8 w U-DICT e U-DICTS r U-TLO t U-TLD

Ergonomic Laboratory Microscope

BX51System Microscope

BX51

BX51-FLBX51

Eyepiece specifications

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Centerable sextuple revolving nosepiece/U-P6RESextuple centerable revolving nosepiece, allowscentration of three objectives.

Septuple revolving nosepiece for DIC/simple POL/U-D7RESeptuple revolving nosepiece with slider slot forDIC/POL.

STAGES

The U-SHG and U-SHGT rubber grip can be attached to the standard stage handle.Different specimen holders are available for use with one slide glass or two, making iteasier to switch specimens with just one hand. As well as a plain stage, there is also arotating model that allows the attachment of a duplex mechanical stage, and agrooved stage which disperses immersion oil to prevent the slide glass from sticking.Users can choose according to application.

Specimen holders

Rotatable graduated stage/U-SRG Plain Stage/U-SP

Oil rectangular stage with right-hand control/U-SVROOil rectangular stage with left-hand control/U-SVLOSpecimen holder/U-HLD-4Rubber grip/U-SHG

OBSERVATION TUBES/EYEPOINT ADJUSTER

REVOLVING NOSEPIECES

Camera adapters

q U-PMTVC w U-PMTV e U-PMTV1Xr U-SMAD t U-CMAD3 y U-TMAD u U-BMAD i U-FMT o U-TV0.25XC!0 U-TV0.5X!1 U-TVZ !2 U-TV0.35XC-2!3 U-TV0.5XC-3!4 U-TV1X-2

!0

!4

!1

!2 !3w

e

r t y

u i

o

q

TR-AdaptersThe single port tube of the trinocular tube is detachable, and can be used with variouscameras through a range of adapters. Using the U-TV1X-2, camera can be useddirectly with no need for a shooting lens. The potential of your microscope is greatlyincreased by its multiple image utilization capabilities.

CAMERA ADAPTERS

ACCESSORIES

A variety of products is available, from widefield binocular tubes to super widefieldtrinocular models. There is also a choice of tilting tubes, including models for invertedimage observation and erect image observation (where the direction of specimenmovement matches the direction of observation image movement).

q

w

t!1

y

ui

o

!0

q Super widefield erect image trinocular tube/U-SWETR w Super widefield trinocular tube/U-SWTR-3 e Trinocular tube/U-TR30-2 r Trinocular tube/U-TR30NIR t Binocular tube/U-BI30-2 y Tilting binocular tube/U-TBI-3 u Ergonomic binocular tube/U-ETBI i Ergonomic binocular tube/U-TTBI o Single port tube/U-SPT !0 Eyepoint adjuster/U-EPA !1 Economic tilting binocular tube/U-CTBI

U-HRD-4

U-HLD-4

U-HLDT-4

U-HLS-4

U-HRDT-4

U-HLST-4

Mechanical stage with left-hand control/U-SVLB-4Specimen holder/U-HRD-4Rubber grip/U-SHG

Mechanical stage with right-hand control/U-SVRB-4Specimen holder/U-HLD-4Rubber grip/U-SHG

Widefield eyepiece/WHN, WHThe WHN maintains image flatness even whenstacking a reflected light illuminator andintermediate attachments.

Super widefield eyepiece/SWHCompatible with F.N. 26.5.

EYEPIECES / PHOTO EYEPIECES

er

Item Name F.N. Diopter Micrometer (ømm)

Widefield WHN10X 22 24WHN10X-H 22 -8 — +5 24WH15X 14 24CROSSWHN10X 22 -8 — +5

Super widefield SWH10X-H 26.5 -8 — +2 —MICROSWH10X 26.5 -8 — +2CROSSSWH10X 26.5 -8 — +2

Finder eyepiece 35WHN10X 22 -8 — +535SWH10X 26.5 -8 — +2PSWH10X 26.5 -8 — +2

* Users who want the SWH10X micrometer: please have your eyepiece adapted by the manufacturer.

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Multi observation bodies/BX2-DO, BX2-SDO, BX2-MDO-5, BX2-MDO-10Olympus discussion systems are invaluable for research studies, lab training andeducation. There is a wide choice, designed for 2 to 10 participants. The pointer ispowered by LED, so there is no concern about sudden lamp failure.

Drawing attachment/U-DAThe drawing attachment projects an image of thepencil and drawing surface into the visual field.Tracing of microscopic structures is made easierand more accurate.

OTHER ACCESSORIES

U-DA

U-DAL10X

Magnification changer/U-ECA, U-ECA1.6XThis intermediate magnification changing compo-nent expands the capability of UIS2 objectives,optimizing the image field without the interruptionof rotating the objective lens; This should be : U-ECA: 1X / 2X, U-ECA1.6X: 1X / 1.6X.

GROUP OBSERVATION SYSTEMS

Arrow pointer/U-APTEnables super imposition of a red or green LEDarrow for display on a monitor or for reproductionwith a photomicrograph.

Dual port/U-DPA double port tube can be combined with thetrinocular tube.

Trinocular intermediate attachments/U-TRU, U-TRUSThis intermediate trinocular attachment can beused simultaneously with the inclinable binocularobservation tube (U-TBI-3). Two light paths areselectable: 100% light for binocular observationor 20% for binocular observation and 80% forimaging through the trinocular port.

Magnification changer/U-CAThis intermediate magnification changingcomponent expands the capability of UIS2objectives, optimizing the image field without theinterruption of rotating the objective lens; 1X / 1.25X / 1.6X / 2X.

BX2-DO

BX2-SDO

BX2-MDO-5

Simple polarizing attachmentSimple polarizing observation can beaccomplished with the combination of U-KPAintermediate attachment for simple polarizingobservation, U-ANT analyzer for transmitted lightand U-POT polarizer.

U-POT

U-KPA

U-ANT

U-DP1XC

U-DP

Approx. 1250mmApprox. 970mmBX2-MDO-10

U-TRUU-TRUS

(unit: mm) (unit: mm)BX41 dimensions BX51 dimensions

(unit: mm) (unit: mm)BX45A dimensions BX45 dimensions

BX41/BX51 specifications

Item BX45A* BX45

Microscope frame Optical system UIS2 optical system

Focus Fixed low stage nosepiece focus15mm focus stroke with coarse adjustment limit stopTorque adjustment for coarse adjustment knobsHigh sensitivity fine focusing knob (adjustment gradations: 1µm)

Illuminator Built-in Koehler illumination for transmitted light6V30W halogen bulb (pre-centered)Light preset switch

Revolving nosepiece Fixed motorized 2-position revolving nosepiece Fixed reversed quintuple nosepiece

Observation tube Widefield (F.N. 22) •Widefield binocular, inclined 30° •Widefield tilting binocular, inclined 5°-35° •Widefield trinocular, inclined 30° •Widefield ergo binocular, inclined 0°-25°

Stage Ceramic-coated coaxial stage with left or right hand low drive control, rotating mechanism and torque adjustment mechanism (Plain, rotating stages are also available)

Condenser Brightfield 4X — 40X (N.A. 0.9) Fixed 3 position universal condenser (N.A. 0.9) 1.25X — 100X (swing out: 1.25X — 2X)

* 4-40X dry type objectives besides UPLSAPO40X are mountable.

BX45/BX45A specifications

283

111 182.4*

326

428.

7*

6545

204

81

15°

415

209

503

412*

187*175

6545

84

34190317.5

Weight: 18kg, Power consumption: 140WThe length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62mm.

Weight: 12kg, Power consumption: 45WThe length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62mm.

Weight: 18kg, Power consumption: 45WThe length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62mm.

Weight: 12kg, Power consumption: 65WThe length marked with an asterisk (*) may vary according to interpupillary distance. Distance for figure shown is 62mm.

326

200.9130 256.1283.9*

14054

1.3*

4512

8

74.5

283328

*401

.7

140

4512

17

*190.378

62°

28370

71

Item BX41 BX51Microscope frame Optical system UIS2 optical system

Focus Vertical stage movement: 25mm stage stroke with coarse adjustment limit stopper, Torque adjustment for coarse adjustment knobsStage mounting position variable, High sensitivity fine focusing knob (minimum adjustment gradations: 1µm)

Illuminator Built-in Koehler illumination for transmitted light Built-in Koehler illumination for transmitted light6V30W halogen bulb (pre-centered) 12V100W halogen bulb (pre-centered)Light preset switch Light preset switch

Light intensity LED indicatorBuilt-in filters (LBD-IF, ND6, ND25 optional)

Revolving nosepiece Interchangeable reversed quintuple/sextuple/septuple nosepieceObservation tube F.N. 18 (for BX41) •Tilting binocular, inclined 30°-60° —

Widefield (F.N. 22) •Widefield binocular, inclined 30° •Widefield tilting binocular, inclined 5°-35° •Widefield trinocular, inclined 30° •Widefield ergo binocular, inclined 0°-25°Super widefield (F.N. 26.5) Super widefield trinocular, inclined 24°

Stage Ceramic-coated coaxial stage with left or right hand low drive control: with rotating mechanism and torque adjustment mechanism, optional rubber grips available (Non stick grooved coaxial, plain, rotatable stages are also available)

Condenser •Abbe (N.A. 1.1), for 4X — 100X•Swing out Achromatic (N.A. 0.9), for 1.25X — 100x (swing-out: 1.25X — 4x)•Achromatic Aplanatic (N.A. 1.4), for 10X — 100X•Phase contrast, darkfield (N.A. 1.1), (phase contrast: for 10X — 100X, darkfield: for 10X — 100X [up to N.A. 0.80])•Universal (N.A. 1.4/0.9), for 2X — 100X (swing-out: 2X — 4X, with oil top lens: 20X — 100X)•Darkfield dry (N.A. 0.8 — 0.92), for 10X — 100X•Darkfield oil (N.A. 1.20 — 1.40), for 10X — 100X•Ultra low (N.A. 0.16), for 1.25X — 4X

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Specifications are subject to change without any obligation on the part of the manufacturer.

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Design and production

adheres to ISO9001

international quality standard.

ISO14001Design and production at the OLYMPUS CORPORATION Ina Plant conforms with

ISO14001 specifications for environmental management systems.

Certification

008

U K A SENVIRONMENTAL

MANAGEMENT

Certified ISO 14001 by

Photos courtesy of:Prof. Tadokoro, Pathology class, School of medicine, St. Marianna University (P.6)Dr. Takanashi, Pathology Dept., Mitsui Memorial Hospital (P.10 above)

Printed in Japan M1541E-0305B