Chill Coma Recovery Time of Hsp70Ba Deletion Drosophila

melanogaster Mutant Compared to Drosophila melanogaster

Joe Geiger and Amit Telwala

Introduction• Background Information• Question: How does the deletion of the

Hsp70Ba gene affect the chill coma recovery time of the mutant compared to D. melanogaster?

• Hypothesis: Due to the deletion of the Hsp70Ba gene, the chill coma recovery time will be much longer for the selected mutant compared to D. melanogaster.

• Null Hypothesis: There will be no difference in the chill coma recovery of the selected Hsp70Ba deletion mutant and D. melanogaster.

Experimental Design• Drosophila melanogaster and

Hsp70Ba deletion Drosophila melanogaster mutant

• D. melanogaster will serve as the control

• 3 vials each containing 10 flies placed into Styrofoam apparatus

• Four trials– 2 for wild-type and 2 for mutant separated by sex

Materials

Experimental Design• The flies (3 vials)

were placed in 4°C for 1 hour and removed to room temperature and video recorded for recovery time

• The results analyzed using paired t-tests through the Statistical Packaging for the Social Sciences (SPSS)

Methods

Results

Results

Results

Results

Discussion• Significant Difference between Chill Coma

Recovery Time of D. melanogaster vs. Hsp70Ba mt

• Confirms from Previous Research: Heat Shock

Protein 70Ba directly affects ability to respond in a

stressful environment

• Unexpected: Trial for Female D. melanogaster

Temperature at 4.5°C

Conclusion• Reject the null hypothesis• Conclude that the Hsp70Ba gene

affects the ability of D. melanogaster for chill coma recovery time

• Future Research: Whether or not the expression of Hsp70 genes are different in flies natural global regions

• Future Research: Working with mutants that have an overexpression of Hsp70Ba to observe chill coma recovery time

References ANDERSON, A. R., HOFFMANN, A. A., & McKECHNIE, S. W. (2005). Response to selection for rapid chill-coma recovery in drosophila melanogaster: Physiology and life-history traits. Genetical Research, 85(1), 15-22.

Macdonald, S.s, L. Rako, P. Batterham, and A.a Hoffmann. "Dissecting Chill Coma Recovery as a Measure of Cold Resistance: Evidence for a Biphasic Response in Drosophila Melanogaster." Journal of Insect Physiology 50.8 (2004): 695-700. Web.

Mockett, Robin J., and Matsumoto, Yuri. “Effect of Prolonged Coldness On Survival and Fertility of Drosophila Melanogaster.” Plos One 9.3 (2014): 1-15. 2014.

ANDERSON, A. R., HOFFMANN, A. A., & McKECHNIE, S. W. (2005). Response to selection for rapid chill-coma recovery in drosophila melanogaster: Physiology and life-history traits. Genetical Research, 85(1), 15-22.

Takahashi, Kazuo H., Phillip J. Daborn, Ary A. Hoffmann, and Toshiyuki Takano-Shimizu. "Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila Melanogaster." Ed. Michael Hendricks. PLoS ONE 6.4 (2011): E17295. Web.