characterization of endothelial cells from hereditary ... · endoglin expression in boecs eng...
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Characterization of Endothelial cells from Hereditary Haemorrhagic Telangiectasia patients.
Africa Fernandez LopezCentro Investigaciones Biológicas
Madrid, Spain
Lab. Dr. Carmelo BernabéuDr. Luisa M. Botella
CONTROL HHT1 HHT2 n HHT2 m
Blood outgrowth endothelial cells characterization
Characterization
90% 2.3
PECAM-1 vW-FENDOGLIN ALK-1 VE-Cad F-Actin FLK1
- Controls- HHT1- HHT2 nonsense- HHT2 missense
CONTROL HHT2 nHHT1 HHT2 mHUVEC
Endoglin expression in BOECs
ENG response upon TGFβtreatment
0
0,5
1
1,5
2
2,5
2.5
1.5 1.5
1
Fold
indu
ctio
n
C
HHT1
HHT2
nHH
T2 m
0
500
1000
1500
2000
Expr
essi
onin
dex
0.5
1
C HHT1 HHT2n
0.6
HHT2m
0.3
ENG
β-Actin
C HHT1 HHT2n HHT2m
Endoglin expression byflow citometry
Endoglin expression
0
0,5
1
ALK-1KR
Fold
indu
ctio
n
0,8 ENG pXP2
pCDNA3 ALK1QD0
0,5
1,5
2
ALK-1WT
ALK1 acts over ENG promoter
**
*
Endoglin-(ZRP-1/Zyxin)-Actin interaction
Actin filament
ZyxinZRP1
ENG
(Conley et al. J Biol Chem. 2004)(Sanz-Rodriguez et al. J Biol Chem. 2004)
20 μm
Control
20 μm 20 μm
20 μm20 μm
HHT1
HHT2n HHT2m
Disorganized cytoskeleton in BOECs
Cytoskeleton disruption by siRNA
Control BOECs
pSUPER-Endo-Ex4 + pEGFP
Cytoskeleton organization recovery
20 μm
20 μm
20 μm
HHT1
HHT2m
20 μm20 μm20 μm
pCMV5-Endoglin + pEGFP
20 μm20 μm20 μm
(Fernandez-L et al. Cardiovasc Res. 2005)
Smad 2/3
ALK5 ALK1
Smad1/5
Proliferation
+-
ENG
+ENG
-
ALK1/ALK5 pathways equilibrium
Mutated ALK1/ENG
Do cells stop proliferating?
MEECsENG +/-
ALK5 (Lebrin et al., EMBO J., 2004)
ALK5
ENG ALK1
C HHT1 HHT2n HHT2m
GAPDH
ALK-5
RT-PCR
BOECs
01020304050
C HHT1 HHT2 n HHT2 m
Arb
itrar
yun
its
Densitometry
TGF-β
ALK5
ACTIVATION PHASERESOLUTION PHASE
ProliferationMigrationInvasion
ProliferationMigrationInvasion
Normal situation: Dynamic equilibrium
ALK1/ ENG
ALK1/ALK5 pathways equilibrium
Mutated ENG or ALK1: Unbalanced equilibrium
TGF-β
ALK5
ACTIVATION PHASERESOLUTION PHASE
ProliferationMigrationInvasion
ProliferationMigrationInvasion
ALK1/ ENG
Decreased ALK5 levels: New steady state
TGF-β
ALK5
ACTIVATION PHASERESOLUTION PHASE
ProliferationMigrationInvasion
ProliferationMigrationInvasion
ALK1/ ENG
ENG
ALK1 ALK5
Non-HHTENG
ALK1 ALK5
ENG
ALK1 ALK5
HHT-1 HHT-2
EndoglinALK-5
EndoglinALK-5
ALK-1 and ALK-5balanced
Altered angiogenesis
HHT PHENOTYPE
Altered ALK1/ALK5 signaling pathways
Decreased ENG levels
Weak cytoskeleton
Dr. Carmelo BernabéuDr. Luisa-María Botella
Dr. Francisco SanzFrancisco Javier BlancoÁfrica Fernández Eva María Garrido
Carmen Langa
This work has been partially supported by theHHT INTERNATIONAL FOUNDATION grant.