Cancer inflammation research applications and products

Flow cytometry | Immunoassays | Cell imaging

Instrumentation Antibodies RNA flow Stimulation

Invitrogen™ Attune™ NxT Flow Cytometer

Conjugated antibodies for flow cytometry

Invitrogen™ PrimeFlow™ assay

Activating and neutralizing antibodies

Better understanding of the complex systems of inflammation and the role they play in cancer is important to oncology research, making improvements in cancer diagnosis and the development of targeted therapies.

With expertise that spans cellular and protein analysis, we offer innovative products that can accelerate inflammation and cancer research. Together, our affordable, high-quality research platforms and reagents are designed to help you reach your cancer inflammation research goals.

Instrumentation Antibodies RNA flow Stimulation

Invitrogen™ Attune™ NxT Flow Cytometer

Conjugated antibodies for flow cytometry

Invitrogen™ PrimeFlow™ assay

Activating and neutralizing antibodies

Cell health reagents Buffers Everyday

essentials

Beads, standards, controls

Buffers and fixation

Apoptosis, proliferation, viability

Cellular phenotyping with our flow cytometry solutionsInvitrogen™ products can help you maximize the power of multicolor flow cytometry for your experiments with the right combination of next-generation instrumentation and high-performance reagents that are validated, optimized, and designed to create bright, beautiful, and consistent results. We make it easier for you to perform great flow cytometry.

Flow cytometry

ELISAsWe offer both coat-it-yourself (uncoated) and ready-to-use (precoated) ELISA formats, with the necessary reagents included to run your assay.

• Broad menu—over 700 cytokine, chemokine, and growth factor targets

• Shorter protocol—time to results in 2.5 to 4 hours (varies by kit)

• Reduced sample—50 µL or less per well, which is less than the leading competitor ELISA kits

Immunoassays

Multiplex assaysOur Invitrogen™ ProcartaPlex™ assays are available as catalog multiplex panels, simplex singleplex sets, and mix and match custom panels for broad flexibility.

• Large screening range—quantitatively measure up to 80 different targets simultaneously

• Large panel menu—ranging from 3-plex to 65-plex, available in human, mouse, rat, and other target species

• Highly flexible—expand or change your biomarker profile as you need with our premixed custom panel option

• Minimal sample volumes—uses as

APRIL BAFF BLC CD30 CD40L ENA-78 Eotaxin

Eotaxin-2 Eotaxin-3 FGF-2 Fractalkine G-CSF GM-CSF GRO alpha

HGF IFN-a IFN-g IL-10 IL-12p70 IL-13 IL-15

IL-16 IL-17A IL-18 IL-1a IL-1b IL-2 IL-20

IL-21 IL-22 IL-23 IL-27 IL-2R IL-3 IL-31

IL-4 IL-5 IL-6 IL-7 IL-8 IL-9 IP-10

I-TAC LIF MCP-1 MCP-2 MCP-3 M-CSF MDC

MIF MIG MIP1a MIP-1b MIP-3a

Find out more about our complete immunoassay portfolio at thermofisher.com/immunoassays

Cytokine profiling with our immunoassaysInvitrogen™ ELISAs and Luminex® multiplex immunoassays can be used for quantitative assessment of soluble proteins such as cytokines, chemokines, growth factors, and other immunological markers.

Featured productProcartaPlex™ Human Immune Monitoring Panel (65 plex)Highest-plexed screening tool on the market for measuring cytokine proteins in

per well to measure 65 different human immune targets. Analytes include:

Cell health reagents Buffers Everyday

essentials

Beads, standards, controls

Buffers and fixation

Apoptosis, proliferation, viability

Antibodies for flow cytometryWe offer over 10,000 conjugated antibodies specifically developed, validated, and manufactured for flow cytometry to investigate CD markers, oncology markers, stem cells, cell signaling, cytokines and chemokines, as well as a multitude of key cell types, such as:

• B cells • Dendritic cells • Embryonic stem cells • Hematopoietic stem cells • Macrophage and monocyte cells

• Mesenchymal stem cells • NK and NKT cells • Neuronal stem cells • T cells • T regulatory cells

Find out more at thermofisher.com/antibodies

• Th1 cells • Th2 cells • Th17 cells • Tfh cells

Attune NxT Flow CytometerSpeed isn’t just about going fast, it’s about discovery. Get high levels of data fidelity 10x faster than ever before.

• Enable more—investigate more sample types, including tumor samples, without losing your precious samples

• Expand your capabilities—grow as your needs expand, with field upgradability of up to 4 lasers and 16 detection channels

• Simplified sample prep—no-wash, no-lyse sample prep options with clog-resistant fluidics

• Option for walk-away automation—plate autosampler with clog-resistant design

• Run samples faster and achieve greater resolution—reduced loss due to clogging

The Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer.

Do more. Find more. Explore more.

Find out more at thermofisher.com/flowcytometry

Excitation laser Channel Antibody labels BlueBlue/violet

Blue/red

Blue/red/

violet

Blue/violet/yellow

Blue/violet/green

Blue/red/

green

Blue/red/

yellow/violet

Blue/red/

violet/green

Violet(405 nm)

VL1 (440/50)

Super Bright 436

● ● ● ● ● ●Alexa Fluor 405

eFluor 450

Pacific Blue

VL2 (512/25)eFluor 506

● ● ● ● ● ●Pacific Green

VL3 (603/48) Super Bright 600

● ● ● ● ● ●Pacific Orange

VL4 Super Bright 645 ● ● ● ● ● ●

Blue(488 nm)

BL1 (525/50)FITC

● ● ● ● ● ● ● ● ●Alexa Fluor 488

BL2 (590/40)PE

● ● ● ● ● ● ● ● ●PE-Alexa Fluor 610

PE-Texas Red

BL3 (695/40)

PE-Alexa Fluor 700

● ● ● ● ● ● ● ● ●

PE-Cy®5

PE-Cy®5.5

PerCP-Cy5.5

PerCP-eFluor 710

BL4 (780/60)PE-Alexa Fluor 700

● ● ● ●PE-Cy®7

Green(532 nm)

GL1 (575/36)Alexa Fluor 532

● ● ●Alexa Fluor 555

PE

GL2 (620/15)PE-eFluor 568

● ● ●Alexa Fluor 594

PE-Texas Red

GL3 (695/40)PE-Alexa Fluor 700

● ● ●PE-Cy5.5

GL4 (780/60) PE-Cy7 ● ● ●

Yellow (561 nm)

YL1 (585/16) PE ● ●

YL2 (620/15)PE-eFluor 610

● ●Alexa Fluor 568

PE-Texas Red

YL3 (695/40)PE-Alexa Fluor 700

● ●PE-Cy5

PE-Cy5.5

YL4 (780/60) PE-Cy7 ● ●

Red(637 nm)

RL1 (670/14)APC

● ● ● ● ●eFluor 660

Alexa Fluor 647

RL2 (720/30)Alexa Fluor 680

● ● ● ● ●Alexa Fluor 700

APC-Alexa Fluor 700

RL3 (780/60)

Alexa Fluor 750

● ● ● ●APC-Alexa Fluor 750

APC-eFluor 780

APC-Cy7

Laser configurations and antibody options for the Attune NxT Flow Cytometer

For Research Use Only. Not for use in diagnostic procedures. © 2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Luminex is a registered trademark of Luminex Corporation. Cy is a registered trademark of GE Healthcare. COL03528 0217

Cell imaging

Count on Invitrogen antibodies

Invitrogen™ antibodies support 9major application areas

Our primary antibodies cover over 85% of the proteome

different colors of Invitrogen™ Alexa Fluor™ dyesfor fluorescently labeled secondary antibodies 24

Find out more at thermofisher.com/antibodies

• Flow cytometry

• Immunofluorescence

• Immunohistochemistry

• ELISA

• Immunoprecipitation

• Western blot and more

>74,000highly validated primary and secondary antibodies, with reactivities for >60 species, and growing

Cellular and tissue staining for protein localizationThe Invitrogen™ EVOS™ FL Auto 2 Imaging System with optional Onstage Incubator is designed to eliminate the complexities of long-term live-cell imaging, enabling researchers to focus on data generation instead of instrument operation and maintenance. It allows for time-lapse cell imaging for long-term monitoring of cell cultures. This flexible, high-performance, and affordable solution for live-cell imaging offers:

• Control—easily control environmental and image acquisition parameters

• High detectability—create time-lapse images of every well of a 96-well plate

• Savings—low cost of ownership and operation

Find out more at thermofisher.com/evos

Find out more at thermofisher.com