brooke towns, loc tran, alec weaver jenna ajello, hayley ...€¦ · power consumption: 660 w...
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SABrSemi-Autonomous Bioreactor
Group 10:
Jenna Ajello, Hayley Fritz, Tyler Le, Ryan Oliver,
Brooke Towns, Loc Tran, Alec Weaver
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Outline1. Hedgehog Concept and Product Overview 2. Sub-System Breakdown3. Competitive Advantages4. Cost and Conclusions
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Hedgehog Concept
❏ SABr optimizes the user experience by offering the most autonomous machine possible❏ Automated liquid handling between commercially available
well plates or conical tubes to custom culture plate❏ Self-sanitizing design reduces customer interference
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Product Overview
❏ Stainless steel dispensing needle and micropump
❏ Customizable well plate ❏ Three unique shaking
patterns❏ OD and FI data of
individual wells
❏ Size: 48 in long X 24 in wide X 24 in tall
❏ Weight: 175 lbs❏ Power Consumption: 660
W maximum
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Product Overview
Microbio Subsystem
Dynamics and Translation Subsystem
Shaking Plates
Sensing and Imaging Subsystem
Heating and Cooling Subsystem
Liquid Handling Subsystem
Structure Subsystem
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Liquid Handling Capabilities
❏ Dispensing mechanism to release desired fluid into well plates
❏ Capable of disposing and neutralizing waste and disinfecting dispensing needle
❏ The TRL for this design is level 3 because system has been demonstrated analytically, but not yet demonstrated in a laboratory environment
❏ OTS parts for this design have a TRL of 9
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Liquid Handling Callout
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Liquid Handling Customer NeedsRequirements Metrics Source Status
2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.
Wattage from micropump, Pump will draw 0.3W
UF Biofoundary
System can operate with provided power source
10. Has a minimal operational lifetime of 10 years.
Off the Shelf pump lifetime of 10 years and reusable stainless steel dispensing needle
UF Biofoundary
System meets minimal operational lifetime
11. Production devices cannot exceed $10,000
This subsystem costs $1,137.66 UF Biofoundary
This cost included purchased parts, manufacturing time, and assembly time. It is about 11.4% of the $10,000 budge
33. Achieve a dispense rate of 300 uL per second
Maximum flow rate of 670 uL UF Biofoundary
Maximum flow rate achieved due to diameter of dispensing needle
34. Deposit the minumum and maximum volume desired by customer
Yes, can deposit minimum and maximum volume of fluid in the range of 5–20,000 µL
UF Biofoundary
Subsystem meets volume requirements desired by customer, due to volume of system, dispensing needle size, and pump precision
35. System can dispose and neutralize waste
UV light used to neutralize waste and then system disposes into waste container
UF Biofoundary
Can properly dispose and neutralize waste
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Microbiological Capabilities ❏ Customized 384 acrylic well plate
❏ Five layer sandwich plate lid
❏ Five flow adjustment valves
❏ Flow meter to control gas flow
❏ The TRL for the microbiology subsystem is a level 2 due to its concept only being formulated and no prototype being completed.
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Microbiological Callout
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Microbiological Customer Needs Requirements Metrics Source Status
2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.
1 Watt maximum from the flowmeter
UF Biofoundry Power can run from the 120V AC electricity from a standard outlet
10. Has a minimal operational lifetime of 10 years.
The flowmeter has a lifetime of 10 years
UF Biofoundry The flowmeter meets the requirement of being a minimum of 10 years.
11. Production devices cannot exceed $10,000.
$1,584.71 UF Biofoundry The cost of this subsystem takes up around 15% of the budget
18. Capable of incubation/hibernation periods from one hour to two weeks
12 Hours of battery lifetime UF Biofoundry Since the system can be kept on if the electricity goes out for 12 hours it should be enough time to get the electricity back on.
21. Can culture microbes in fully enclosed compartments or vessels that are easily interchangeable and that allow for media exchange.
SABr has one closed loop system that is enclosed with a sandwich lid and interchangeable with different containers.
UF Biofoundry This closed loop system can culture for fully enclosed vessels and allows for media exchange.
22. Accommodate existing culture plates of the following sizes: 6, 24, 48, 96, deep 96, 384.
With the customized well plate, it can hold the maximum size, 384.
UF Biofoundry Since the maximum size well plate is used for the customized well plates all of the other sizes can fit inside the system.
23. Accommodate existing conical tubes of the following sizes: 15mL & 50mL.
The translation system includes a section for conical tubes of both sizes.
UF Biofoundry The system does accommodate for the two different sizes of conical tubes.
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Heating/Cooling Capabilities
❏ Evenly Distributed Convection Heating and Cooling
❏ Easily Removable from the Structure❏ Closed Loop Control❏ symmetric design increases ease of
manufacturing❏ TRL 3 design has been proven
analytically, but a prototype has not been made. The component are at a TRL 9.
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Heating/Cooling Component Callout
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Heating/Cooling Customer NeedsDESIGN REQUIREMENTS METRIC SOURCE STATUS
2. If powered, run from 120V
AC electricity from a standard
wall outlet with a 15-amp
breaker.
The subsystem uses a maximum of 487.8 W
to maintain a constant temperature which is
27% of the total power
UF
Biofoundry487.8 W (27%)
1. Production device cannot
exceed $10,000.The subsystem cost $1,839.06
UF
Biofoundry$1,839.06
19 Can perform culture
condition closed loop control.
The system includes a temperature sensor
with closed loop control to control the
power into the peltier coolers.
UF
Biofoundry
DESIGN MEETS
REQUIREMENT
24. Uniformly heats wells/tubes
to prevent condensation.
Using a convection heating and cooling
reduces the temperature gradient between
the air and well plates.
UF
Biofoundry
DESIGN MEETS
REQUIREMENT
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Sensing Capabilities
❏ OD and FI measurements through using mCherry filter cube set, a photomultiplier tube, and a separate phototransistor
❏ Technology Readiness Level 3❏ Concept validated experimentally❏ OTS parts evaluated individually
meet Level 9
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Sensing Components Callout
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Sensing Customer RequirementsREQUIREMENT METRIC SOURCE STATUS
2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.
40 Watts maximum UF Biofoundry System can successfully operate with the given power source
10. Has a minimal operational lifetime of 10 years. 11 years UF Biofoundry System lifetime exceeds requirement
11. Production devices cannot exceed $10,000. $2,295.77 UF Biofoundry System takes almost 25% of budget
13. Programmable: control parameters can be changed, or more complex control can be added
Use of software like BioTek to aid dynamics system
UF Biofoundry Variable options to exceed customer requirement
14. Have an intuitive user interface Laptop connected to system omits handling of microscope slides
UF Biofoundry Autonomous experience using simple customer interference
28. Can measure OD and FI in all individual wells of any accommodated well plate readings to provide culture condition data
2 separate sensors for separate readings
UF Biofoundry Successfully measures both OD and FI
29. Designed with respect to trade-offs in using monochromators vs. filter cubes for FI readings
mCherry filter cube set UF Biofoundry Allows lower cost and higher sensitivity
30. Achieve a light intensity of 1 kW/cm2 at 640 nm for several minutes. High frequency wavelengths would operate at a lower intensity as it becomes lethal to cells.
600 W/cm^2 at 643 nm UF Biofoundry Lowered intensity at higher frequency allows for a factor of safety when reading live cells
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Dynamics and Translation System❏ 3-axis motion
capabilities
❏ Up to three unique shaking patterns
❏ Closed loop control with integrated limit switches for fail-safe system
❏ Technology Readiness Level 2
❏ OTS parts evaluated individually meet Level 9
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Dynamics and Translation System
Y-Axis Assembly
X-Axis Assembly
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Dynamics and Translation System
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Dynamics and Translation System Requirements
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Overall Structure Capabilities
❏ The full-seamed weldment is made of 5052 aluminum with 2 access openings which are sealed to prevent contamination from external impurities
❏ The TRL is 2 for the overall structure because the concept has not yet been prototyped.❏ Changes are expected to occur once a physical product is made for testing
because the interaction between the customer and the product can’t be 100% predicted via models and formulas
24”
24”48”
3/32 O-ring per Parker spec for face seal
Parker 2-265 O-ring gland seal
Quick-Access port: utilized for insertion of cell cultures as well as quick cleaning operations
Hinged lid: utilized for assembly of internal components, maintenance, and deep sanitization operations
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Lid
Structure weldment
Overall Structure - Component Callout
Quick access flange
Data acquisition laptop
LED indicators and E-stop
Lid latch
Lid hinge 2x
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Overall Structure - Requirements Table
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Custom Culture Plate
Design Highlights and Key Features
Multiple Access Points
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Cost Analysis
OTS PART COST$6,607.31
MATERIAL AND MANUFACTURING COST
$4,611.96
TOTAL PROTOTYPE COST
$11,552.34
ASSEMBLY COST$318.03
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Why Choose SABr?
Conclusions
The SABr is the ultimate solution to customer needs,
providing a mostly autonomous experience that is not
currently available for the price point.
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We would like to thank our corporate sponsors Northrop Grumman and Cummins, faculty and staff for attending our presentation and supporting this project.
Thank you
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