Brain Research

Methods.

By Rachel Tierney.

Direct Brain Stimulation Techniques.

• Direct brain stimulation consists of a device that delivers a weak electrical impulse to disrupt neurons in specific areas of the brain whilst the patient is fully aware and conscious.

• Two types – Electrode stimulation and Transcranial Magnetic Stimulation (TMS).

• Direct brain stimulation techniques only identify the function of the brain.

Electrode Stimulation.

• Wilder Penfield’s research in the 1940’s to 1960’s established that when certain areas of the brain were stimulated by electric currents a part of the patient’s body would react. He then used this information to help treat epileptic patients.

• http://www.youtube.com/watch?v=kNdM9JhTPJw

An electrode is a small fine wire that can be placed onto or inserted into specific areas of the brain.

Electrode StimulationAdvantages.

• Helped create a ‘map’ of the brain.

• Provided a way for researchers to identify the locations and functions of brain areas, including hemispheric specialisation.

• Generally very consistent results

Limitations.• Extremely invasive.• Considered

unacceptable with today’s ethical standards.

• Difficulty in generalising results as most studies were done on epileptic patients with not normally functioning brains.

Transcranial Magnetic Stimulation (TMS)

• TMS is a direct brain stimulation technique that involves directing a magnetic field pulse through the skull and then disrupts neuron activity in specific areas of the cerebral cortex.

• Single pulse TMS & Repetitive TMS (rTMS)• rTMS induces consecutive impulses that cause neurons to lose

their ability to fire.

Transcranial Magnetic Stimulation (TMS)

Advantages• Non–invasive.• Completely safe and

harmless.• Allows researchers to

conduct research ethically on human participants.

• Provides information about function of brain.

Limitations• Can’t be used on patients

with any metal or implanted medical devices in their body.

• rTMS may induce scalp pain or mild headaches.

• Very rare cases of inducing seizures.

• Only penetrates the part of the brain directly below the skull.

Electroencephalograph (EEG)

• Electrodes are placed on the scalp(cleaned) in numerous positions to identify the neuron activity in different areas of the cerebral cortex.

• Detects, amplifies and records electrical brain activity.

Electroencephalograph (EEG)

Advantages• Non-invasive and

harmless.• Extremely cost

effective compared to other methods.

• Provides information about the function of the brain even whilst the participant is doing long tasks.

Limitations• Only provides general

information.• Difficult to identify

precise areas of brain activity.

• The skull weakens the electrical activity.

Structural Neuroimaging Techniques.

• Structural neuroimaging techniques can capture detailed images of the brain structure and anatomy.

• Two types – CT(computerised tomography) & basic MRI(magnetic resonance imaging)

Computerised Tomography (CT)

• A.K.A – Computerised axial tomography (CAT).• This technique uses computer technology to take

snapshots of the brain which is separated into ‘slices’. • An injection of iodine called contrast is injected to

highlight the blood vessels in the brain.• The patient must lie very still as their head is inserted

into the scanner which rotates to take pictures from different angles.

http://www.youtube.com/watch?v=M-4o0DxBgZk – Apply this to the brain instead of the body!

Computerised Tomography (CT)

Advantages• Rarely has side effects

and is non-invasive.• Shows brain structure.• Useful to identify

location and size of tumours.

Limitations• Very expensive

procedure• Does not show

functionality of the brain on the structure.

Magnetic Resonance Imaging (MRI)

• Is a structural neuroimaging technique similar to the CT that uses magnetic fields and radio waves to vibrate atoms in neurons to project an image of the brain.

• Colour is used on the image to identify highly active neurons which resemble high brain activity and vice versa.

Magnetic Resonance Imaging (MRI)

Advantages• Clearer, more

sensitive and more detailed than a CT.

• Non-invasive and harmless.

• Almost photographic quality images.

• Does not use radioactive substances or X-rays.

Limitations• Only shows structure.• People with metal

implants in their body cannot use it.

Functional Neuroimaging Techniques.

• These are techniques that can identify not only the brain structure like the CT and MRI can but also they have the ability to show the functions of the brain ‘at work’. • Three types – PET(Positron Emission Tomography), SPECT(single photon emission computed tomography) and fMRI(functional magnetic resonance imaging).

Positron Emission Tomography (PET)

• PET uses radioactive tracer which is either injected or consumed to track blood flow through measuring how much glucose is being used by neurons in a particular part of the brain.

• A computer-generated image shows the brain ‘at work’.

Positron Emission Tomography (PET)

Advantages• Can clearly show brain

tumours, PET is more sensitive than CT and MRI.

• Enables researchers to establish the role of the brain in behaviour and mental processes.

• Shows function and structure.

Limitations• PET scans are less

detailed than MRI scans.

• Radioactivity decays rapidly.

• Doesn’t always pick up rapid changes in brain activity due to 40 second intervals.

http://www.youtube.com/watch?v=5KXIDUo18aA

Single Photon Emission Computed Tomography (SPECT)

• SPECT is very similar to PET but it uses a longer lasting radioactive tracer.

• A scanner and computer construct either two or three dimensional images of the brain activity.

Single Photon Emission Computed Tomography (SPECT)

Advantages• Longer lasting

radioactive tracer can detect brain activity on longer tasks.

• Shows both function and structure.

• Non-invasive.• Significantly less

expensive than PET.

Limitations• Images are not as

good as PET and have low resolution.

Functional Magnetic Resonance Imaging (fMRI)

• fMRI machines use oxygen levels in the blood of the brain to determine the activity of certain areas when the brain is ‘at work’.

• fMRI can take rapid pictures of the brain and therefore capture more brain activity and changes.

Functional Magnetic Resonance Imaging (fMRI)

Advantages• Does not require

radioactive substances.

• Colour coding and detailed images make it relatively easy to read.

Limitations• Levels of activity may

not be related to the tasks that they are asked to complete.

• Costly.