biotransformation in plant tissue culture and animal cell culture
TRANSCRIPT
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Group Members
Khushbkht Khan Sehrish Khan Huma Iqbal Maryum Batool Muryum Farooq
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Biotransformation In Plant Tissue Culture and Animal Cell Culture
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Biotransformation
"Biotransformation is the process whereby a substance is changed from one chemical to another (transformed) by a chemical reaction within the body”.
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HOW???
Chemical reaction: Hydroxylation. Oxidation (of hydroxyl group), Reduction (of carbonyl group), Hydrogenation, Glycosyl conjugation, Hydrolysis.
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Hydroxylation
By hydroxylases.
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Oxidation
By oxidases
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Reduction
By Reductase
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Hydrogenation
Transferases Isomerases Reductases
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Hydrolysis
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Glycosyl conjugation
By transferases
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Biotransformation in Plant tissue culture
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Plants
cellulose, wood, and rubber.
terpenoids, cardenolides, coumarins,anthraquinonesflavonoids, glucosinolates, alkaloids
drugs, flavors, pigments (food ingredients), agrochemicals
Basic
materials
Secondary metabolites
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PROBLEM
the formation and the accumulation of some secondary metabolites does not normally occur in the cultured cells of higher plants.
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SOLUTION
The biotransformation of exogenous substrates by the plant cultured cells.
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Case StudyLavandula angustifolia
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Lavandula angustifolia Mill.
a shrub with a well-developed woody base,
growing to a height of about 2 ft, A native of the West Mediterranean
region
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Oil Of Lavender Anti-inflammatory, anxiolytic, mutagenic activities analgesic property natural repellant, pharmaceutical and industrial auxiliaries
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Cell suspension culturing MS media MT media SH media
Main Growth Regulators: 2,4-D IAA Kinetin Sucrose Coconut water Vitamin complexes
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Study of biotransformation Exogenous Compounds:
octanal, citronellal, linalyl acetate, bornyl acetate, geranyl acetate, linalool, borneol, citronellol menthol.
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ester hydrolysis and oxidation were the main reactions which occurred in
biotransformation process.
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The data in this work indicates that the natural metabolite
monoterpenoids such as linalool were not converted but linalool with
added functional groups (linalyl acetate) was efficiently transformed
using cells.
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Biotransformation in animal cell cultures
A Case Study
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2-hydroxyethyl-methacrylate(HEMA)
Greatly found on unsterilized dental material.
Hazards: I. Contact dermatitis in dentists.II. Inhalation has potential effects on
lung cells.
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The main CYP-metabolizing capacity of the lungs is found in the bronchiolar Clara cells and alveolar type 2 cells.
Lung diseases such as lung cancer caused by chemical pollutants are often suggested to originate from these cells.
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Biotransformation
Beneficial as toxic compounds are inactivated and removed from the body.
However, biotransformation of xenobiotics sometimes results in the formation of reactive metabolites that may cause severe damage to cellular molecules.
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Biotransformation enzymes which results in HEMA toxicity in lungs is
tested.
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Culture and media
Alveolar type 2 cells are cultured on Williams E medium epidermal growth factor Insulin Hydrocortisone
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Analysis through
Immunofluorescence RT-PCR microscopy
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HEMA induces apoptotic like morphology in primary cultures of rat alveolar type 2 cells by a different mechanism.
the increased toxicity could be expressed via metabolic activation by the CYP2E1 enzyme.
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Conclusion