biotech product guide
TRANSCRIPT
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Quality atmdi 02
Innovative Technologies for Nucleic Acid Purification 03
Plasmid DNA Isolation Kits 04
Selection Chart 04
Process Flow 05 - 07
FastLyse pDNA Miniprep Kit 08
pDNA Miniprep Kit 09
Express 96 Miniprep pDNA Kit 10
Quanta Midi Kit 11
Quanta Maxi Kit 12
Express Midi Kit 13
Express Maxi Kit 14
Genomic DNA Isolation Kit 15
Selection Chart 15
Process Flow 16
gDNA Miniprep Kit 17
Plant gDNA Miniprep Kit 18
PCR Purification & Gel Extraction Kits 19
Selection Chart 19
Process Flow 20-21
PCR Purification Kit 22
Micro PCR Purification Kit 23
Nano PCR Purification Kit 24
Gel Extraction Kits 25
Micro Gel Extraction Kit 26
Nano Gel Extraction Kit 27
Binding membranes for Molecular Biology 28
Membrane Syringe Filters 31
Bottle Top Filters 32
Contents Page
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mdi
Certificate of Quality
Membrane Technologies
Head of Quality Control
Issue Date: 03-May-2010
Advanced Microdevices Pvt. Ltd.21, Industrial Area, Ambala Cantt, INDIA,
Tel: +91-171-2699290/ 2699274
Fax: +91-171-2699221/ 2699008Website: www.mdimembrane.com
Email: mdi @vsnl.com
An ISO 9001:2000 Company
The Fastlyse pDNA Miniprep Kit has beenmanufactured in mdi facility in compliance with ISO9001
regulations using validated productionprocesses.
LOT RELEASE CRITERIA
Binding Capacity of the column
: 50g
pDNAYields
DNAPurity(A260 /A280) : 1.8-2.0
FASTLYSE pDNA MINIPREP KIT
Catalog No. : FLPKXXXXXXX0250Type : FLPKLot No. :
DNase Free : Yes
Plasmid type pUC vectorsCopy number-High (500-700)Fragment size -9 kbElution volume 50l
VALIDATED FOR
Downstream Processing
Restriction Digestion Ligat ion PCR Sequencing Bacterial Transformation Cloning
Shelf Life
Minimum one year at Room Temperature More than two years at 2-8 C
Purification of low copy number plasmids (
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Innovative Technologies for Nucleic Acid Purification
Page 03mdi PRODUCT GUIDEBIOTECH
mdi introduces a major breakthrough in the field of nucleic acid purification through
its latest research. These special kits offer extremely high yields in much reduced
process time while effectively addressing other user concerns such as purity, yields,
consistency and shelf life.
Unique Performance Advantages
uFastest pDNA isolation in 10 minutes.
uNo pellet formation and resuspension steps.
uWorks with very low culture volumes upto 600l.
uVery high binding capacity of upto 50g.
uVery high yields with low elution volumes.
uVery high within lot and inter lot consistency.
Unique Performance Advantages
uFastest large scale pDNA Isolation in just 30 minutes
uLesser number of operating steps
uVery high binding capacity
uNo precipitation required for desalting
uHigher yields with less culture volumeuHighly concentrated, ultrapure pDNA isolation in very low elution volumes
uHigh within lot as well as lot to lot consistency
Unique Performance Advantages
uFastest DNA Purification in just 5 minutes
uHigh DNA Recovery from even low feed quantities
uHighly concentrated DNA in very low elution volumes
uEasy purification of large sized fragments without shearing
uSuitable for all type of downstream applications
mdiFastlyse pDNA Miniprep Kits
mdiQuanta Kits
mdiNano PCR & Gel Extraction Kits
0
50
100
150
200
250
Culture Volume
35ml 100 ml
Yield(g)
Higher Yields with Less Culture Volume
Higher Yields with Less Culture Volume
0
2
4
6
8
10
12
14
16
0.61.5
Yield(g)
Culture Volume (ml)
mdi
Competitor Q
High Concentration Recovery
0
20
40
60
80
100
120
61.6 ng 107.6 ng 422.7 ng
Different Feed
Recovery%
Elution Volume : 5l
mdi
Competitor Q
New
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Selection Chart
Plasmid DNA Isolation Kits
Plasmids are extra chromosomal DNA molecules capable of replicating
independently of the chromosomal DNA. This genetic material is ubiquitous in
bacteria and because of its special ability to move genes from cell to cell, has become a
versatile tool for both researchers and scientists involved in life sciences research.
There are several concerns related to researchers such as yields, purity, processingtime, binding capacity, consistency and shelf life.
mdipDNA isolation kits offer many unique advantages with respect to all these issues.
The following selection chart will help you choose the most suitable kit for your
application.
Page 04 mdi PRODUCT GUIDEBIOTECH
FastLyse pDNA MiniprepKit
1.8-2.0 600l 5 ml Upto 25g 10 minutes?In-Vitro Transcription
?In-Vitro Translation
?High quality sequencing
?Cloning
?Probe Generation
?PCR
?Restriction Digestion
?Bacterial Transformation
?Ligation
?Transfection
?Gene Silencing
?Microinjection
?Library construction
pDNA Miniprep Kit 1.8-2.0 1 ml -5ml Upto 25g 30 minutes
Express 96 pDNAMiniprep Kit 1.8-2.0 1 ml -5ml
Upto25g/well 45 minutes
Quanta Midi Kit 1.8-2.0 25 35 ml Upto 250g 20 minutes
Quanta Maxi Kit 1.8-2.0 100-130ml Upto 1000g 25 minutes
Express Midi Kit 1.8-2.0 50-75 ml Upto 200g
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Small Scale Plasmid DNA Kits
mdi Fastlyse pDNA Miniprep Kit
( upto 25g)
mdi pDNA Miniprep Kit
( upto 25g)
mdi Express 96 pDNA Miniprep Kit
( upto 25 g/well)
Plasmid DNA Purification Kits: Process Flow
Page 05mdi PRODUCT GUIDEBIOTECH
Elute
Overnight BacterialCulture
Bind & Wash
Lyse & Neutralize
Ultra Pure pDNA
Elute
Dry
Overnight BacterialCulture
Bind
Wash
ResuspendLyseNeutralize
Ultra Pure pDNA
ResuspendLyseNeutralize
Pelleted Bacteria
Filter
ExpressFilter Plate
Bind
Vacuum
ExpressPrep Plate
Wash
Vacuum
Elute
Vacuum
Vacuum
Microtubesfor Ultra
Pure pDNA
Tube Rack
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Page 06 mdi PRODUCT GUIDEBIOTECH
mdi Quanta Midi Kit
( upto 250g)
mdi Quanta Maxi Kit
( upto 1mg)
Pelleted Bacteria
Alkaline Lysis
Clear Lysate
By Filtration
Add Binding Buffer
Bind/Wash
VacuumPump
Dry
Elute
Ultra PurePlasmid DNA
Pelleted Bacteria
Alkaline Lysis
Clear Lysate
By Filtration
Add Binding Buffer
Bind/Wash
VacuumPump
Dry
Elute
Ultra PurePlasmid DNA
Large Scale Plasmid DNA Kits
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mdi PRODUCT GUIDEBIOTECH Page 07
Resuspend Pelletin Solution AL-1
Add buffer AL-3 and wait
Filter intoMidi column
VacuumPump
Bacterial Pelletby centrifugation
Cell lysis inSolution AL-2
Pass clear lysateby applying
vacuum
Wash and dryagain by vacuum
VacuumPump
Eluteby vacuum
Add Buffer P
Pass throughprecipitator
VacuumPump
Dry and eluteby centrifugation
Ultra pure
concentratedPlasmid DNA
Resuspend Pelletin Solution AL-1
Add buffer AL-3 and wait
Filter intoMaxi column
VacuumPump
Bacterial Pelletby centrifugation
Cell lysis inSolution AL-2
Pass clear lysateby applying
vacuumWash and dry
again by vacuum
VacuumPump
Eluteby vacuum
Add Buffer P
Pass throughprecipitator
VacuumPump Dry and elute
by centrifugation
Ultra pureconcentrated
Plasmid DNA
mdi Express Maxi Kit
(upto 750g)
mdi Express Midi Kit
(upto 200g)
Large Scale Plasmid DNA Kits
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ORDERING
INFORMATION
FastLyse pDNA Miniprep KitThe Next Level in pDNA Isolation ( In just 10 minutes)
High Yields
0
510
15
20
25
30
35
0.6 1.5 3 4.5
Culture Volume (ml)
Elution Volume: 50l
Yield(g)
Very High Binding Capacity
0
10
20
30
40
50
60
mdi Competitor Q
BindingCap
acity(g)
Type XX XX XX X Pack Size
Type Code Pack Size Code
FLPK FLPK 50 0050
250 0250
1000 1000
Example: FLPK XX XX XX X 0250
Page 08 mdi PRODUCT GUIDEBIOTECH
Unique Performance AdvantagesuFastest pDNA isolation in 10 minutes
uWorks with very low culture volumes (600l)
uNo pellet formation and resuspension steps
uVery high binding capacity of upto 50guVery high yields with low elution volumes
uVery high within lot and inter lot consistency
Unique TechnologyDirect pDNA isolation from 600l culture without any pelleting and resuspension.
A unique soft pellet formation step allows ideal lysis conditions resulting in very
high yield even with larger culture volumes upto 5ml, helps reduce process time
and makes it ideal for plasmid screening.
ApplicationsuLibrary construction
uRestriction Digestion
uCloning
uLigation
uBacterial Transformation
uPCR
uAutomated Sequencing
uProbe Generation
uMicroinjection
u
u
u
u
u
u
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280
Specifications
Purity
pDNA Binding Capacity : 50 g
pDNA Yield : Upto 25 g
pDNA Size : Upto 25 kb
Minimum Culture Volume : 600 l
Elution Volume : > 30 l
Total Time Taken : 10 minutes
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ORDERING
INFORMATION
mdi PRODUCT GUIDEBIOTECH Page 09
pDNA Miniprep Kit(In Less Than 30 Minutes)
Unique Performance AdvantagesuHigh Binding Capacity > 25g
uHigh pDNA yields and Purity
uHighly Concentrated Yields in low elution volumes
uNo Precipitation required for desaltinguHigh within lot and lot to lot consistency
ApplicationsuAutomated Fluorescent Sequencing
uRadioactive Sequencing
uRestriction Digestion
uTransformation / Transfection
uCloning
uPCR
uLigation
uProbe Generation
uMicroinjection
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280
Specifications
Purity
pDNA Binding Capacity : > 25 g
pDNA Yield : Upto 25 g
Culture Volume : 1-5 ml
Minimum Elution Volume : > 30 l
Total Time Taken : 25 minutes
Type XX XX XX X Pack Size
Type Code Pack Size Code
MIPK MIPK 50 0050
250 0250
1000 1000
Elution volume vs pDNA Recovery and Concentration
0
200
400
600
800
1000
0
10
2030
40
pDNAConc.(
ng/l)
pDNARecove
ry(g)
30 50 100 150
Elution volume(l)
pDNA Recovery
pDNA Conc.
Culture Volume vs. pDNA Recovery and Concentration
0
200
400
600
800
0
5
10
15
20
25
30
0 1 2 3 4 5 6 7
Culture Volume(ml)
Elution Volume: 50l
pDNA Recovery
pDNA Conc.pDN
AConc.
(ng/l)
pDNARecovery(g)
Incubation time vs. Recovery
10
30
5070
90
110
1 2 3 4 5 10 30
Incubation time (min)
50l
100l%R
ecov
ery
Example: MIPK XX XX XX X 0250
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ORDERING
INFORMATION
Page 10 mdi PRODUCT GUIDEBIOTECH
Express 96 Miniprep pDNA Kit(In Less Than 45 Minutes)
Type XX XX XX X Pack Size
Type Code Pack Size Code
EMPK EMPK 4 0004
24 0024
High Consistency: Well to Well
0
4
8
12
16
20
12 24 36 48 60 72 84 96
Culture Volume: 1.5ml
Yield(g/well)
PurityUltrapure Plasmid DNA: A / A = 1.8-2.0260 280
Example: EMPK XX XX XX X 0024
Unique Performance AdvantagesuMultiple (96) samples processing
uVery high binding capacity
uNo precipitation required for desalting
uHighly concentrated, ultrapure pDNA isolation in very low elution volumesHigh within lot as well as lot to lot consistency
ApplicationsuRestriction Enzyme Digestion
uLibrary Screeining
uIn vitro Translation
uSequencing
uLigation
uTransformation / Transfection
uPCR
SpecificationspDNA Binding Capacity/Well : > 25 g
pDNA Yield/Well : upto 25 g
Culture Volume/Well : 1-5 ml
Minimum Elution Volume/Well : 75 l
Total Time Taken : 45 minutes
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mdi PRODUCT GUIDEBIOTECH Page 11
Quanta Midi Kit(In Just 20 Minutes)
Type
Type Code
QDPK QDPK
XX XX XX X Pack Size
Pack Size Code
25 0025
100 0100
Example: QDPK XX XX XX X 0100
Unique high binding
capacity midi spin column
Vacuum based,
no gravitational wetting
Concentrated pDNA,
no Isopropanol precipitation
Unique Performance AdvantagesuFastest Midiprep for pDNA Isolation
uVery high binding capacity - Upto 400g
uNo gravitational waiting
uSimple filtration to remove cell debris; No centrifugationuNo precipitation required for concentration
uHigher yields with low culture volume
uHighly concentrated, ultrapure pDNA isolation in very low elution volumes
uHigh within lot as well as lot to lot consistency
Unique TechnologyCombines the use of a specially designed filtration devices and tube extender to
obtain high purity pDNA in just 30 minutes. No desalting is required to obtain
ultrapure pDNA yields.
ApplicationsuAutomated Sequencing
uRestriction Digestion
uCloning
uTransfection / Transformation
uPCR
uLigation
uMicroinjection
uProbe Generation
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280Purity
Yield & Binding Capacity
0
50
100
150
200
250
300
350
400
450
Yield
Binding Capacity
mdi Competitor Q
(g)
Specifications
Low Copy
Plasmid
High Copy
Plasmid
Binding capacity of membrane ( ds DNA)
Recovery
Maximum culture volumes
400 g 400 g
80% 80%
25-35 ml 50 ml
Expected yield of plasmid 150-250 g 100-200 g
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ORDERING
INFORMATION
Quanta Maxi Kit(In Just 25 Minutes)
Page 12 mdi PRODUCT GUIDEBIOTECH
Unique Performance AdvantagesuFastest Maxiprep for pDNA Isolation
uVery high binding capacity - Upto 1200 g
uNo gravitational waiting
uSimple filtration to remove cell debris; No centrifugationuNo precipitation required for concentration
uHigher yields with low culture volume
uHighly concentrated, ultrapure pDNA isolation; in very low elution volumes
uHigh within lot as well as lot to lot consistency
Unique TechnologyCombines the use of a specially designed filtration devices and tube extender to
obtain high purity pDNA in just 30 minutes. No desalting is required to obtain
ultrapure pDNA yields.
ApplicationsuAutomated Sequencing
uRestriction Digestion
uCloning
uTransfection / Transformation
uPCR
uLigation
uMicroinjection
uProbe Generation
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280PurityYield & Binding Capacity
0
300
600
900
1200
1500
mdi Competitor Q
Yield
Binding Capacity
Unique high binding
capacity spin maxi column
Type XX XX XX X Pack Size
Type Code Pack Size Code
QXPK QXPK 10 0010
25 0025
Example: QXPK XX XX XX X 0025
Specifications
Low Copy
Plasmid
High Copy
Plasmid
Binding capacity of membrane ( ds DNA)
Recovery
Maximum culture volumes
1200 g
80% 80%
130 ml 200 ml
Expected yield of plasmid upto 1000 g upto 250 g
1200 g
(g)
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Page 13mdi PRODUCT GUIDEBIOTECH
Express Midi Kit(Less Than 1 Hour)
Type XX XX XX X Pack Size
Type Code Pack Size Code
MDPK MDPK 25 0025
100 0100
Unique Performance AdvantagesuFast pDNA isolation
uVery high binding capacity - Upto 350g
uHigh pDNA yields and purity
uHighly concentrated yields in very low elution volumes
ApplicationsuAutomated Sequencing
uRestriction Digestion
uCloning
uTransfection / Transformation
uPCR
uLigation
uMicroinjection
uProbe Generation
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280
Purity
Yield and Binding Capacity
0
100
200
300
400
Elution Volume (ml)
mdi Competitor Q
Yield
Binding Capacity
Example: MDPK XX XX XX X 0100
Specifications
Low Copy
Plasmid
High Copy
Plasmid
Binding capacity of membrane ( ds DNA)
Recovery
Maximum culture volumes
350 g
80% 80%
50 ml 100 ml
Expected yield of plasmid 100-200 g 30-100 g
350 g
(g)
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ORDERING
INFORMATION
Specifications
Low Copy
Plasmid
High Copy
Plasmid
Binding capacity of membrane ( ds DNA)
Recovery
Maximum culture volumes
750 g
80% 80%
150 ml 250 ml
Expected yield of plasmid 300-750 g 50-250 g
750 g
Express Maxi Kit(Less Than 1 Hour)
Unique Performance AdvantagesuFast pDNA isolation
uVery high binding capacity - Upto 750g
uHigh pDNA yields and purity
uHighly concentrated yields in low elution volumes
ApplicationsuAutomated Sequencing
uRestriction Digestion
uCloning
uTransfection / Transformation
uPCR
uLigation
uMicroinjection
uProbe Generation
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280
Purity
Precipitation
Filtration ofCulture Debris
DNA Elution by Vacuum
0
150
300
450
600
750
900
Elution Volume (ml)
Yield & Binding Capacity
Yield
Binding Capacity
mdi Competitor Q
Type XX XX XX X Pack Size
Type Code Pack Size Code
MXPK MXPK 10 0010
25 0025
Example: MXPK XX XX XX X 0025
Page 14 mdi PRODUCT GUIDEBIOTECH
(g)
Final Elution of Concentrated DNA
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Page 15mdi PRODUCT GUIDEBIOTECH
Sample Types PurityAmount of Starting
MaterialYields
Processing
TimeApplications
Mammalian
Tissue
- Liver- Kidney- Lung- Spleen- Mouse Tail
1.8-2.0 25mg 10 -30g < 1hr
?In-Vitro Transcription
?In-Vitro Translation
?Automated Sequencing
?Cloning
?Southern Blotting
?AFLP
?RFLP
?RAPD
?PCR
?Restriction Digestion
?Transformation
?Ligation
?Transfection
?SNP Genotyping
?Microsatellite analysis
?RT-PCR
Animal Blood 1.8-2.0 200l Upto 12g
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gDNA Miniprep Kits
mdi Genomic DNA Miniprep Kit mdi Plant gDNA Miniprep Kit
Genomic DNA Isolation Kits: Process Flow
Page 16 mdi PRODUCT GUIDEBIOTECH
Sample
Bind
Wash 2X
Elute
Ultra Pure gDNA
Dry with open lid
Lyse
Plant Tissue
Bind DNA
Wash 2X
Elute
GrindLysePrecipitate
Centrifuge through
mdi Shredder
Add Binding Buffer andtransfer to mdi plantmini spin column
Ultra Pure gDNA
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Page 17mdi PRODUCT GUIDEBIOTECH
gDNA Miniprep Kit( for Mammalian Tissue, Blood, Bacterial Cells and Cultured Cells )
Unique Performance AdvantagesuVery high binding capacity - Upto 50g
uHigher yields
uNo precipitation step for high purity gDNA.
uSuitable for all type of downstream applications
ApplicationsuPCR
uSouthern Blotting
uRAPD Analysis
uAFLP Analysis
uRFLP Analysis
uIn- Vitro Transcription
uRestriction Digestion
uTransformation
uTransfection
uSNP Genotyping
uMicrosatellite Analysis
uRT - PCR
uGene Silencing
uMicroinjection
uProbe Generation
Binding capacity of membrane (ds DNA) : 50 g
Recovery : 80%
Minimum elution volume : 200l
Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280
Specifications
Purity
Maximum Amount of Tissue :25 mg9
Maximum bacterial cells : 2 x 10Maximum volume of blood sample : 200l
6Maximum amount of cultured cells : 5 x 10
Capacity of column reservoir : 700l
Highly Concentrated Yields
0
10
20
30
40
50
60
mdi Competitor Q
Second Elution
First Elution
Yield(g)
Marke
r
Thermus
therm
ophil
us
Marke
r
Thermus
aquaticus
EcoliD
h5
Agarose gel analysis of gDNA fromdifferent bacteria purified with mdi
gDNA Miniprep Kit . M: lambda-HindIII
Elution Volume: 200l/Elution
ORDERING
INFORMATION
Type XX XX XX X Pack Size
Type Code Pack Size Code
CTGK CTGK 50 0050
250 0250
1000 1000
Example: CTGK XX XX XX X 0250
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ORDERING
INFORMATION
Plant gDNA Miniprep Kit
Unique Performance AdvantagesuVery high binding capacity - Upto 50g
uHigher yields with different plant tissue
uNo precipitation step for high purity gDNA
Suitable for all type of downstream applications
ApplicationsuPCR
uSouthern Blotting
uRAPD Analysis
uAFLP Analysis
uRFLP Analysis
uIn- Vitro Transcription
uRestriction Digestion
uTransformation
uTransfection
uSNP Genotyping
uMicrosatellite Analysis
uRT - PCR
uGene Silencing
uMicroinjection
uProbe Generation
Ultrapure Plasmid DNA: A / A = 1.7-1.9260 280
Specifications
Purity
Maximum Amount of Starting Material :100 mg wet weight, 20 mg dry weight
Capacity of column Reservoir : 700lBinding capacity of membrane (ds DNA) : 50 g
Recovery : 80%
Elution volume : 100l
Page 18 mdi PRODUCT GUIDEBIOTECH
Highly Concentrated Yields
0
3
6
9
12
15
mdi Competitor Q
Yield(g)
( Tomato Leaves)
gDNA: Yield
Different Samples
Tomato Potato Spinach Wheat Chickpea
Rice
0
5
10
15
20
25
Yield(g)
Fungus
Type XX XX XX X Pack Size
Type Code Pack Size Code
PTGK PTGK 50 0050
250 0250
1000 1000
Example: PTGK XX XX XX X 1000
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DNA Isolation Kits Purity DNA QuantityRecovered
DNA Fragment
Elution
VolumeApplications
PCR Purification Kit 1.8-2.0 5-10g 100bp -10kb 30l
?Transfection
?In-Vitro Transcription
?
In-Vitro Translation
?High quality sequencing
?Microarray Analysis
?Cloning
?PCR
?Gene Silencing
?Probe Generation
?Microinjection
?Restriction Digestion
?Demanding enzymaticmodifications
?Library Construction
?Bacterial Transformation
?Ligation
Micro PCRPurification Kit
1.8-2.0 1-2g 70bp -4kb 10l
Nano PCRPurification Kit
1.8-2.0 upto 400ng 70bp -4kb 5l
Gel Extraction Kit
1.8-2.0
5-10g
70bp -10kb 30l
Micro GelExtraction Kit
1.8-2.0 1-2g 70bp -4kb 10l
Nano GelExtraction Kit
1.8-2.0 upto 400ng 70bp -4kb 5l
PCRPurificati
onKits
GelExtractionKits
Selection Chart
PCR Purification and Gel Extraction Kits
Page 19mdi PRODUCT GUIDEBIOTECH
mdi offers PCR Purification Kits for efficient removal of contaminants such as primers,
enzymes and salts and Gel Extraction Kits to purify very small quantities of even large
fragment size DNA from upto 400mg of gel slices.
The following selection chart will help you choose the most suitable kit for yourexperiment.
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Page 20mdi PRODUCT GUIDEBIOTECH
PCR Purification Kits
mdi PCR Purification Kit
(5-10g)
mdi Micro PCR Purification Kit
(1-2g)
mdiNano PCR Purification Kit
(upto 400ng)
PCR Reaction
Bind
Wash
Add 5 volumes ofbuffer MPB
Elute
Ultra PureDNA Fragment
Dry
PCR Reaction
Bind
Wash
Add 5 volumes ofbuffer NPB
Elute
Ultra PureDNA Fragment
Dry
PCR reaction
Bind
Wash
Add 5 volumes ofbuffer PB
Elute
Ultra PureDNA Fragment
Dry
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Gel Extraction Kits
mdi Gel Extraction Kit
(5-10g)
mdi Micro Gel Extraction Kit
(1-2g)
mdi Nano Gel Extraction Kit
(upto 400ng)
SolubilizedGel Slice
Bind
Wash
Elute
Ultra Pure DNAFragment
Dry
mdi PRODUCT GUIDEBIOTECH Page 21
Solubilized
Gel Slice
Bind
Wash
Elute
Ultra Pure DNA
Fragment
Dry
Solubilized
Gel Slice
Bind
Wash
Elute
Ultra Pure DNA
Fragment
Dry
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ORDERING
INFORMATION
PCR Purification Kit(5-10g)
Unique Performance AdvantagesuEasy purification of large sized fragments without shearing
uNo precipitation step for high purity DNA
uHighly concentrated DNA yields in low elution volume
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Total eluate volume : 28 l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800l
Binding capacity of membrane (ds DNA) : 10 g
Recovery : 90-95%Recovered DNA fragment : (100 bp -10 kb)
Minimum elution volume : 30 l
Page 22mdi PRODUCT GUIDEBIOTECH
Complete primer removal using
mdi PCR Purification Kit
a: Before purification b: After purification
M: Marker
1000 bp
200 bp
Primer
M Ma b a b
Type XX XX XX X Pack Size
Type Code Pack Size Code
SPCK SPCK 50 0050
250 0250
1000 1000
Example: SPCK XX XX XX X 0250
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ORDERING
INFORMATION
Micro PCR Purification Kit(1-2g)
Unique Performance Advantages uEasy purification of large sized fragments without shearing
uNo precipitation step for high purity DNA
uHighly concentrated DNA yields in very low elution volume
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Total eluate volume : 9 l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800l
Binding capacity of membrane (ds DNA) : 5 g
Recovery : 80%Recovered DNA fragment : (70 bp - 4 kb)
Minimum elution volume : 10 l
Page 23mdi PRODUCT GUIDEBIOTECH
High Concentration Recovery
0
20
40
60
80
100
120
mdi Competitor Q
Feed: 1g
%R
eco
very
Type XX XX XX X Pack Size
Type Code Pack Size Code
MPCK MPCK 50 0050
250 0250
1000 1000
Example: MPCK XX XX XX X 0250
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INFORMATION
Page 24 mdi PRODUCT GUIDEBIOTECH
Nano PCR Purification Kit(upto 400ng))
Unique Performance AdvantagesuWorks with very small DNA quantities
uFastest DNA Purification in just 5 minutes
uHigh DNA Recovery and yield from even low feed quantities
uHighly concentrated DNA in very low elution volumeuEasy purification of large sized fragments without shearing
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Total eluate volume : 4l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800lBinding capacity of membrane (ds DNA) : 5 g
Recovery : 80-85%
Recovered DNA fragment : (70 bp-4 kb)
Minimum elution volume : 5l
High Concentration Recovery
0
20
40
60
80
100
120
61.6 ng 107.6 ng 422.7 ng
Feed
%R
ecovery
Elution Volume : 5l
Type XX XX XX X Pack Size
Type Code Pack Size Code
NPCK NPCK 50 0050
250 0250
1000 1000
Example: NPCK XX XX XX X 0250
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Page 25mdi PRODUCT GUIDEBIOTECH
Gel Extraction Kit(5-10g)
Unique Performance AdvantagesuEasy purification of large sized fragments without shearing
uNo precipitation step for high purity DNA
uHighly concentrated DNA yields in low elution volume
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Minimum elution volume : 30 l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800l
Maximum weight of gel slice : 400mg
Binding capacity of membrane (ds DNA) : 10 gRecovery : 70-80 %
Recovered DNA fragment : (70 bp-10 kb)
Type XX XX XX X Pack Size
Type Code Pack Size Code
SGEK SGEK 50 0050
250 0250
1000 1000
0
2040
60
80
100
50 100 150 200 250 300 350 400
Gel Weight (mg)
Elution Volume (30 l)
Gel weight vs. % Recovery
%R
ecovery
High recovery using mdi Gel Extraction Kit
B: Before extraction 1-2: After extraction
M: Marker
1000 bp
200 bp
M 1 2 B
Example: SGEK XX XX XX X 1000
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Page 26 mdi PRODUCT GUIDEBIOTECH
Micro Gel Extraction Kit(1-2g)
Type XX XX XX X Pack Size
Type Code Pack Size Code
MGEK MGEK 50 0050
250 0250
1000 1000
Unique Performance AdvantagesuEasy purification of large sized fragments without shearing
uNo precipitation step for high purity DNA
uHighly concentrated DNA yields in very low elution volume
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Minimum elution volume : 10 l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800l
Maximum weight of gel slice : 400mg
Binding capacity of membrane (ds DNA) : 5 gRecovery : 80 %
Recovered DNA fragment : (70bp-4 kb)
Highly Concentrated Recovery
0
15
30
45
60
75
90
mdi Competitor Q
%Re
covery
Feed: 1g
Example: MGEK XX XX XX X 0250
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Page 27mdi PRODUCT GUIDEBIOTECH
Nano Gel Extraction Kit(upto 400ng)
Unique Performance AdvantagesuFastest DNA Purification
uVery high DNA binding capacity
uHigh DNA Recovery and yield from even low feed quantities
uHighly concentrated DNA ; in very low elution volumesuEasy purification of large sized fragments without shearing
ApplicationsuTransfection
uIn-Vitro Transcription
uIn-Vitro Translation
uHigh quality sequencing
uMicroarray Analysis
uCloning
uPCR
uGene Silencing
uProbe Generation
uMicroinjection
uRestriction Digestion
uDemanding enzymatic modifications
uLibrary Construction
uBacterial Transformation
uLigation
Minimum elution volume :
Total eluate volume : 4l
Ultrapure Plasmid DNA: A / A =1.8-2.0260 280
Specifications
Purity
Capacity of column reservoir : 800lBinding capacity of membrane (ds DNA) : 5g
Recovery : 80-85 %
Recovered DNA fragment : (70bp-4 kb)
5l
Type XX XX XX X Pack Size
Type Code Pack Size Code
NGEK NGEK 50 0050
250 0250
1000 1000
Example: NGEK XX XX XX X
0
20
4060
80
100
54.6 ng 128.3 ng 392.8 ng
Feed
%Re
covery
Elution Volume : 5 l
0250
High Concentration Recovery
120
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120
105
0
50
100
150
SCN
0.2m
0.45m
2
(g/cm
)
Protein binding capacities of SCN membranes with BSA
mdi binding membranes are uniform, paper thin, white plastic supports, having
specially designed porous structures and binding sites suitable for transfer and
hybridization of biological molecules.
mdi offers a wide range of binding membranes viz. Nitrocellulose, Nylon-66, andPVDF, exhibiting a range of properties to suit various applications.
Special featuresuHigh binding capacities of the transferred molecules
uGood wettabilty for Nitrocellulose and Nylon-66 membranes
uPVDF membranes are hydrophobic
uAbility to retain the molecules without affecting its biological activity
uChemical compatibility and mechanical durability
uAbility to be blocked by simple procedures
uHigh signal to noise ratio
For special applications,mdioffers internally supported binding membranes which
exhibit very high mechanical strength.
Nitrocellulose Membrane Type - SCN and SCNJ
SCN
Pure nitrocellulose membrane produced specially for life sciences applications.
SCNJ
Internally supported to offer superior handleability.
CharacteristicsuHigh binding capacities for proteins and nucleic acid molecules
uMinimum background: High signal to noise ratio
uUniform and easy wettability
uCan be blocked by normal blocking methods
uDoes not bind common protein stains
uCompatible with colorimetric, radiolabelled, chemiluminescent, fluorescent,
and staining detection methods
ApplicationsuProtein blotting
uDot and slot blots
uNucleic acid dot/slot blots
uColony/plaque lifts
uEnzyme immunoassays
PackagingSealed in aluminum bags to maintain hydrophilicity and high protein binding on
prolonged storage. The separators are of pure polyester film to avoid any
contamination of the membrane from the separator.
Binding Membranes for Molecular Biology
Page 28 mdi PRODUCT GUIDEBIOTECH
Protein Binding
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Page 29mdi PRODUCT GUIDEBIOTECH
mdi Polyvinylidene fluoride (PVDF) membrane is a naturally hydrophobic support
matrix which offers much higher protein binding capacities than that of
Nitrocellulose membrane and also binds difficult to bind proteins such as
glycoproteins.
CharacteristicsuVery high binding capacities
uMinimal background: High signal to noise ratio
uRemains flexible and non-brittle after processing
uChemically resistant to harsh reagents making it a convenient matrix for
protein sequencing
uHigher strength than pure nitrocellulose
uCompatible with all types of detection methods
ApplicationsuIdeal support matrix for protein sequencing
uFor high performance, reproducible western blotting
uFor protein staining, glycolipid detection and immunoblotting
Nylon-66 Membrane Type - SNNP and SNNPZ
SNNP: Internally supported Nylon-66 membranes
SNNPZ: Positively charged for enhanced binding of negatively charged molecules
Characteristics
uVery high binding capacities for nucleic acid moleculesuEasy wettabilty
uUltraviolet cross linkable
uChemically resistant; tolerant to alkali fixation
ApplicationsuNucleic acid transfers
uDots/slot blots
uColony/plaque lifts
uMultiple reprobing
PVDF Membrane - Type SVF
240
190
120105
0
50
100
150
200
250
0.45 0.2
2
(g/
cm
)
SVF
SCN
Pore Size ( )m
Protein binding capacities of SVF and SCN membranes with BSA
Protein Binding
ORDERING
INFORMATION
Pore Size XX XX Sterile/ Non Sterile Pack Size
Pore Size Code Code Pack Size Code
0.2m 01 Non Sterile 1 25 11
0.45m 02 50 03
Roll 01
Size
Dia Code
82mm 13
90mm 14
137mm 20
142mm 16
80mm x 100mm 88
150 x 150mm 87
200 x 200mm 86
300 x 300mm 85
3M X 300mm 84
3M x 240mm 833M x 100mm 81
* SVF, SNNP and SNNPZ are available in a maximum width of 240 mm
** SCNJ is available in a maximum width of 100mm
SCNJ 13 02 XX XX 03
Example:
1
Type
Type Code
SCN SCNX
SCNJ SCNJ
SVF SVFX
SNNP SNNP
SNNPZ SNPZ
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Page 30 mdi PRODUCT GUIDEBIOTECH
SVFSNNPZ
HR
R
R
R
HR
R
R
R
RR
HR
R
R
R
P
P
P
R
R
NR
SNNP
This selection chart highlights the suitability of various mdi membranes for differentapplications based on their properties.
SCNJ
HR
HR
R*
NR
NR
HR
R
R
R
R
RR
P
R
R
R
R
R
R
R
HR = Highly Recommended
R = Recommended
R* = Recommended for Proteins only
P = Possible
NR = Not Recommended
Membrane Type
Colony or Plaque lift
Detection Method
Molecule Fixation
Molecule Removal
Transfer Method
Alkaline Transfer
Electrotransfer
UV Crosslinking
Biomolecules
Nucleic Acids
Capillary Blot
Alkali Fixation
Radiolabelled
Luminesence
Vacuum Blot
Colorimetric
Fluorescence
Reprobing
Proteins
Dot Blot
Staining
Multiple
BakingDrying
Once
Selection Chart
HR
HR
HR
NR
R
R
R
R
R
R
RR
R
R
R
P
P
P
R
R
NR
NR
HR
NR
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
SCN
R
HR
R
HR
R*
R
R
NR
RR
P
NR
HR
R
R
R
R
NR
NR
NR
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Page 31mdi PRODUCT GUIDEBIOTECH
Type Size Pore Size Inlet/Outlet X Bell Sterile/ Non Sterile Pack Size
Type Code Dia Code Pore Size Code Code Size Code Pack Size Code
SYPL SYPL 4mm 01 0.2m 01 Female Luer Lock M With Bell B Non Sterile 1 100 04
SYKG SYKG 13mm 03 0.45m 02 Male Luer Slip N Without Bell X Sterile 2 12 08
IKG IKGX 25mm 06 1/4 SHB* B
Pre-Sterilized Polyethersulfone Membrane Syringe Filters
SYPL-S Pre-sterilized syringe filters are available in pore sizes of 0.2m and 0.45m
and several diameters to suit various applications for filtration in the laboratory.
Special Types Available
SY25KG-S
This special syringe filter houses a microglassfiber pre-filter along with the
Polyethersulfone membrane filter and is ideal for filtering difficult to filter solutions.
IKG-S
This 50mm Polyethersulfone syringe filter with 1/4 stepped hose barb connections
incorporates large filtration area and microglassfiber pre-filter for larger volume
filtration. These filters can be used with 50ml syringe to filter volumes up to 500ml.
Special FeatureExtremely low protein binding exhibited by mdi syringe filters results in very high
product recovery.
99.9% 98%
0
50
100
SYPL Competition
BSA
Note: only IKG-S filters are available with bell
Example: SYKG 06 01 MN X X 2 04
Specifications
Pore Size 0.2m, 0.45m
Diameter 4mm
EFA* 0.07cm
Hold-Up Volume 7 for Br. diminuta
0.45m: LRV >7 for Sr. marcescens
13mm
0.8cm
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Page 32 mdi PRODUCT GUIDEBIOTECH
Vacufil-S: Pre-Sterilized Bottle Top Vacuum Filters
mdi Pre-Sterilized Bottle Top Vacuum Filtration units with an extra large 75mm
diameter, low protein binding Polyethersulfone membrane are the best option for
filtration of biologicals like sera and culture media, and other proteinaceous solutions.
Vacufil filters have a hydrophobic filter in the vacuum arm to prevent passage offiltrate to the pump. These filters screw perfectly on to vacuum safe bottles with
45mm neck size.
FeaturesuLow protein binding
uExtra large filter area
uHigh flow rates
u100% Integrity tested
uNo elastomers or adhesive used in sealing
uNon-toxic materials of construction
0.2m, 0.45m
75mm
45mm (Screw cap neck)
7 for Br. diminuta
SpecificationsPore Size :
Diameter :
Connection :
Hold-Up Volume :
Retention Efficiency :
Type Size Pore Size XX XX Sterile/ Non Sterile Pack Size
Type Code Dia Code Pore Size Code Code Pack Size Code
Vacufil VFPX 75mm 11 0.2m 01 Sterile 2 12 08
0.45m 02 24 12
Example: VFPX 11 01 XX XX 2 12
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