biosynthesis some alkaloids. piperine
TRANSCRIPT
Biosynthesis some alkaloids
Piperine
indole
Isolasi and Identifikasi
Indole Alkaloids1- Ergot Alkaloids
• Occurrence:Ergot is the dried sclerotium of a fungus, Claviceps purpurea (Fam. Hypocreacea) that arise on the ovaries of the rye plant (Secale cereale, Fam. Gramineae).
• Consumption of flour contaminated with Ergot led to many serious intoxications known as (Ergotism- Ignis Fire) in Europe.
• Ergot can be detected in flour by using UV light where contaminated flour will show violet spots.
• Classification of Ergot Alkaloids:
A- Clavine Type Alkaloids:Simple water soluble bases with little medicinal value. All end with “clavine: e.g. Agroclavine.
B- Lysergic acid Amides:They are all derivatives of (l)-Lysergic acid and subclassified into:
1- Simple lysergic acid amides:
Composed of Lysergic acid and simple amines.
2- Polypeptide Alkaloids:
Composed of Lysergic acid and at least 3 amino acids.
• General Characters: Ergot alkaloids are N-monosubstituted amide derivatives of both
lysergic acid and its isomer isolysergic acid that differ only in configuration at C-8.
On treatment with ammonia lysergic and isolysergic acids give the corresponding amides ergine and isoergine respectively.
Members related to lysergic acid (e.g. ergotamine and ergometrine) are levorotatory, more active and designated by suffix “ine”.
Members related to isolysergic acid (e.g. ergotaminine and ergometrinine), are dextrorotatory, less active and designated by suffix “inine”.
NH
N CH3
COOH
10
8
NH
N CH3
COOH
NH
N CH3
CONH2
Lysergic acid Isolysergic acid Ergine
1- Simple Lysergic acid amides• Characters:
1- Composed of Lysergic acid and simple amines.
2- Low molecular weight. 3- Water Soluble.
Ergonovine (Ergometrine)
Composed of (l)-lysergic acid and 2-aminopropanol. Its (d) isomer is called Ergometrinine.
• Uses:It causes vigorous contraction of the uterus.
It is mainly used as an oxytocic in order to aid
delivery or to prevent postpartum hemorrhage.
NH
N CH3
COHNHCH2C
10
8
CH3OH
Ergonovine (l) (Ergometrine)Ergonovinine (d) (Ergometrinine)
==
Lysergic acid diethylamide (LSD)• It is a semisynthetic product.• LSD has potent CNS stimulant effect.• LSD is one of the abused drugs.
NH
N CH3
CON
10
8
C2H5
C2H5
2- Polypeptide Alkaloids• Characters:
They are derivatives of Lysergic acid with a complex polypeptides of at least 3 amino acids.
They have high molecular weight.
They are insoluble in water.
This class include medicinally important members.
Ergotamine• Characters: Its (d) isomer is called Ergotaminine. The peptide moiety is composed of 3 amino acids:
-Hydroxyalanine
Proline
Phenylalanine
• Uses:Treatment of migraine as it constricts
the peripheral blood vessels.
Has some oxytocic (ecobolic) activity.
NH
N CH3
CO
O
N
N
OHCH3
CH2
O
O
10
89
Structure Activity Relationship:
• Lysergic acid must be in the (l) form. The (d) isomers are inactive.
• Saturation of the 9- 10 double bond of Ergotamine gives Dihydroergotamine, a compound with antimigraine effect but no oxytocic effect.
NH
N CH3
CO
O
N
N
OHCH3
CH2
O
O
10
89
Stability:• The active (l) form convert to the (d) isomer by the effect of
Alkalis or prolonged storage in alcohol.
• Addition of water to the 9- 10 double bond takes place in aqueous acidic solutions, upon exposure to day or UV light. The resulted Lumi alkaloids are inactive.
N CH3
C
O
N CH3
C
N CH3
C
O
10
8
10
8
10
8H H
R RR
HO
(l) form (d) form
N CH3
C
O
10
8H
R
HO
• Tests for identification:
Van-Urk's Reagent (p-dimethyl aminobenzaldehyde (PDAB) in 15% H2SO4, containing traces of FeCl3) + Alkaloid → Deep blue color.
Erlich Reagent (p-dimethyl aminobenzaldehyde (PDAB) in H2SO4) + Alkaloid → Deep blue colour.
Keller’s test: Solution of the alkaloids in acetic acid with traces of FeCl3 + concentrated H2SO4 on the wall of the test tube → blue layer is formed
between the two phases.
2- Vinca (Catharanthus) Alkaloids
Occurrence:Occurrence:Catharanthus or Vinca is the dried whole plant of Catharanthus or Vinca is the dried whole plant of
Catharanthus roseusCatharanthus roseus G. Don (or G. Don (or Vinca roseaVinca rosea L), Fam. L), Fam. Apocynaceae. Apocynaceae.
It contains about 150 alkaloids, the most important It contains about 150 alkaloids, the most important are vinblastine and vincristine.are vinblastine and vincristine.
• Classification:1- Monomeric Alkaloids:
These are alkaloids that contain either indole or indoline:
Indole monomers e.g. Catharanthine Indoline monomers e.g. Vindoline and Vincamine.
2- Dimeric Alkaloids: Homogenic dimmers: Composed of two indole or
indoline monomers. Mixed dimmers: One indole and one indoline
monomers e.g. Vincristine and Vinblastine.
1- Monomeric Alkaloids:
• VincamineEnhances the cerebral blood flow, facilitate cerebral circulation metabolism and increase general activity. Vincamine is used in cerebral vascular deficiency and atherosclerosis in elderly patients.
NN
H
C2H5OHH3COOC
N
NH
H3COOC
C2H5
N
N
CH3
COOCH3OH
H3COOC
Vincamine Catharanthine Vindoline
2- Dimeric Alkaloids: Mixed dimmers
• These are dimeric alkaloids having indole and indoline (dihydro-indole) nuclei e.g. Vinblastine and Vincristine
• Vinblastine and Vincristine They occur in very minute amounts in Vinca (0.003- 0.005); 500 Kg of
the plant yield only 1 gm of vincristine.
They are very important for cancer treatment.
Vincristine is more active but isolated in smaller amounts than Vinblastine. Vinblastine can be converted to vincristine chemically or by microbial transformation using Streptomyces albogriseolu .
Structures:• Vinblastine (Vincaleukoblastine) is produced by coupling of Catharanthine
and Vindoline.
• Vincristine (leurocristine) has CHO istead of CH3 in the vindoline part of Vinblastine.
N
N
R
COOCH3OH
H3COOC
N
NH
H3COOC HO
R=CH3 VinblastineR=CHO Vincristine
• Tests for identification:
1-Vanillin /HCl reagent gives with:
Vinblastine a pink color.
Vincristine an orange-yellow color.
2-Van-Urk's reagent:
→ Reddish-brown color.
• Uses : Vinblastine is used for treatment of Hodgkin's disease
(Pseudoleukemia or Lymphatic anaemia) and carcinoma resistant to other therapy.
Vincristine has a cytotoxic effect .It is useful in the treatment of leukemia in children, small cell lung cancer, cervical and vaginal cancers.
• Mechanism: Both alkaloids are Antimetabolites interfere with the syntheses
of Desoxyribonucleic acids.
Semisynthetic derivatives:
• Vindesine: It is used for treatment of acute lymphoid
leukemia in children.
• Vinorelbine: It is an oral anticancer with broader activity and lower neurotoxicity than vinblastine.
N
N
CH3
COOCH3OH
H2NOC
N
NH
H3COOC HO
N
N
CH3
COOCH3OH
H3COOC
N
NH
H3COOC HO
3- Physostigma (Calabar beans) Alkaloids• Source: Physostigma venenosum.
• Constituents: Physostigmine (Eserine).
• Properties: It is a tertiary base, possessing an ester linkage. Contains 3 Nitrogen atoms. Eserine on alkaline hydrolysis Eseroline +
Methylamine + CO2
NN
O
MeMe
Eserine
Me
H
O
Me-HN
NN
HO
MeMe
Eseroline
Me
H
+ CH3NH2 + CO2
Alkaline Hydrolyses
Eserine upon oxidation, in presence of trace of alkali e.g. NaOH, or traces of metals is transformed to rubreserine (red compound). Therefore, it could be affected by the alkalinity of glass containers during storage.
• Tests for identification: Eserine blue test: Eserine + strong ammonia solution → Yellowish
red color , evaporation → Bluish residue soluble in alcohol (eserine blue).
Eserine + alkali hydroxides → Red color.
NN
HO
MeMe
Me
H
Eseroline
NN
O
MeMe
Me
H
O
Rubreserine
oxidation
• Uses:
It has a cholinergic effect and stimulates glands’ secretion (c.f. atropine) act by inhibition of choline-esterase. So used in poisoning by Solanaceous Alkaloids.
Treatment of Alzheimer’s disease due to cholinergic effect .
Eserine is mainly used as a myotic drug in the treatment of glaucoma.
Diaphoretic in cases of kidney dysfunction.
4- Nux-Vomica Alkaloids
• Source: – Seeds of Strychnose nux vomica and Ignatus beans.
• Constituents: 5% Alkaloids mainly Strychnine and Brucine.
• Properties: Brucine is the dimethoxy derivative of Strychnine. Both alkaloids contains 2 Nitrogen atoms. Hemitoxiferine is a degradation product of
strychnine. Dimerization of hemitoxiferine produces a valuable skeletal muscle relaxant Toxiferine.
N
N
OO
R
R
R= H StrychnineR= OCH3 Brucine
• Tests for identification:• Nitric acid test:
Drops of concentrated nitric + few crystals of the alkaloids: Strychnine gives a faint yellow color that on evaporation turns to yellow color Brucine gives an intense red color, that on evaporation and addition of SnCl2 solution turns to violet.
• Tests for strychnine: Sulfuric acid-dichromate test:
Few crystals of strychnine + drops concentrated H2SO4 + few crystals of K2Cr2O7 → deep blue streaks → violet → purplish red → orange → yellow.
Test with Mandalin's reagent:Strychnine gives Deep violet blue color, add water → red → cherry-red.
• Uses:Strychnine is extremely toxic. It is used in veterinary medicine as CNS stimulant and tonic. It is used as antidote in barbiturate poisoning. It is also used as rodenticide.
Brucine is less toxic than strychnine. It is sometimes used as CNS stimulant, Commercially it is used as alcohol and oil denaturant
Vincristine
OverviewOverview
• I. Physical PropertiesI. Physical Properties
• II. SynthesisII. Synthesis
• III. CharacterizationIII. Characterization
• IV. History and UsesIV. History and Uses
• V. How it worksV. How it works
General InformationGeneral Information22
• CAS Number: 57-22-7CAS Number: 57-22-7
• Molecular Formula: CMolecular Formula: C4646HH5656NN44OO1010
• Molecular Weight: 824.97Molecular Weight: 824.97
• Melting Point: 218-220Melting Point: 218-220°C°C
• Clear liquidClear liquid
(taken by injection)(taken by injection)
Synthesis from a Natural Synthesis from a Natural SourceSource
• Vincristine has two natural sources: Vincristine has two natural sources: Catharanthus roseus (Vinca rosea), the Catharanthus roseus (Vinca rosea), the Madagascar periwinkle, andMadagascar periwinkle, and
Tabernaemontana pachysiphonTabernaemontana pachysiphon
• This yield from the Madagascar periwinkle This yield from the Madagascar periwinkle is less than 1is less than 1μμg/g (.0003%)g/g (.0003%)66
Synthesis from vinblastineSynthesis from vinblastine
H2CrO4
Ethyl Acetate2 hours, -83- -
25°C
80-90% yield5
• This is the easiest and most common method of synthesis, as well as one can give high yields
Synthetic ProductionSynthetic Production
Discovered in 2004First route to make synthetic vincristine6
SpectroscopySpectroscopy
• Mass SpectrumMass Spectrum44: M+1=825: M+1=825
• UV SpectrumUV Spectrum11: 220, 255, 296 nm: 220, 255, 296 nm
• IR SpectrumIR Spectrum66: 3445, 2924, 2852, 1740, : 3445, 2924, 2852, 1740, 1681, 1597, 1498, 1458, 1369, 1230, 1681, 1597, 1498, 1458, 1369, 1230, 1033, 747 cm-1 1033, 747 cm-1
History and UsesHistory and Uses
• Discovered in 1953 by Robert L. Noble, Discovered in 1953 by Robert L. Noble, and cleared by the FDA in 1963 as and cleared by the FDA in 1963 as Oncovin, marketed by Eli Lilly and Co.Oncovin, marketed by Eli Lilly and Co.
• Used to treat acute leukemias and all Used to treat acute leukemias and all lymphomaslymphomas
• Side effects include nervous system Side effects include nervous system damage and constipationdamage and constipation
• Work is being done to develop more Work is being done to develop more effective derivatives with fewer side effective derivatives with fewer side effectseffects88
How it worksHow it works
• Stops division of cellsStops division of cells
• Enters cell during mitosis and blocks Enters cell during mitosis and blocks formation of microtubules of the mitotic formation of microtubules of the mitotic spindle during metaphasespindle during metaphase3,73,7
BibliographyBibliography
• (1) Anonymous Vincristine. In (1) Anonymous Vincristine. In The Merck index: an encyclopedia of chemicals, drugs, The Merck index: an encyclopedia of chemicals, drugs, and biologicals; and biologicals; O'Neil, M. J., Ed.; Merck: Whitehouse Station, NJ, 2006; pp 1717-O'Neil, M. J., Ed.; Merck: Whitehouse Station, NJ, 2006; pp 1717-1718. 1718.
• (2) Anonymous Chemfinder.com Database and Internet Searching. (2) Anonymous Chemfinder.com Database and Internet Searching. http://chemfinder.cambridgesoft.com (accessed Jan. 22, 2007). (accessed Jan. 22, 2007).
• (3) Anonymous Overview: The Mitotic Spindle. (3) Anonymous Overview: The Mitotic Spindle. http://www.sinauer.com/pdf/nsp-cellcycle-6-0.pdf (accessed Apr. 15, 2007). (accessed Apr. 15, 2007).
• (4) Choi, Y. H.; Kim, J.; Yoo, K. Supercritical Fluid Extraction and Liquid (4) Choi, Y. H.; Kim, J.; Yoo, K. Supercritical Fluid Extraction and Liquid Chromatography-Electrospray Mass Analysis of Vinblastine from Chromatography-Electrospray Mass Analysis of Vinblastine from Catharanthus roseusCatharanthus roseus. . Chem. Pharm. Bull. Chem. Pharm. Bull. 20022002, 50, 1294--1296. , 50, 1294--1296.
• (5) Huhtikangas, A.; Laakso, I.; Seppaenen-Laakso, T.; Verma, A. Patent Application (5) Huhtikangas, A.; Laakso, I.; Seppaenen-Laakso, T.; Verma, A. Patent Application Country: Application: FI; Patent Country: FI Patent 107924, 2001. Country: Application: FI; Patent Country: FI Patent 107924, 2001.
• (6) Kuboyama, T.; Yokoshima, S.; Tokuyama, H.; Fukuyama, T. Stereocontrolled total (6) Kuboyama, T.; Yokoshima, S.; Tokuyama, H.; Fukuyama, T. Stereocontrolled total synthesis of (+)-vincristine. synthesis of (+)-vincristine. Proc. Natl. Acad. Sci. U. S. A. Proc. Natl. Acad. Sci. U. S. A. 20042004, 101, 11966-11970. , 101, 11966-11970.
• (7) Lurie, P. M.; Manaster, J.; Stryckmans, P. A.; Vamecq, G. Mode of Action of (7) Lurie, P. M.; Manaster, J.; Stryckmans, P. A.; Vamecq, G. Mode of Action of Chemotherapy Chemotherapy in vivoin vivo on Human Acute Leukemia--II. Vincristine. on Human Acute Leukemia--II. Vincristine. Europ. J. Cancer Europ. J. Cancer 19731973, 9, 613--620. , 9, 613--620.
• (8) Szabo, L.; Bolcskei, H.; Eszter, B.; Mak, M.; Szantay, C. Synthesis of vinca (8) Szabo, L.; Bolcskei, H.; Eszter, B.; Mak, M.; Szantay, C. Synthesis of vinca alkaloids and related compounds, Part XCV: Nitration study of vinblastine-type alkaloids and related compounds, Part XCV: Nitration study of vinblastine-type bisindole alkaloids. bisindole alkaloids. Arch. Pharm. Pharm. Med. Chem. Arch. Pharm. Pharm. Med. Chem. 20012001, 334, 339--405. , 334, 339--405.
Quinoline
Quinoline AlkaloidsCinchona Alkaloids
• Cinchona bark contain many alkaloids the majors are:
1- Quinine and Quinidine.
2- Cinchonine and Cinchonidine.• Cinchona alkaloids are present
as salts with Quinic and
Cinchotannic acids.
• They are diacidic bases form two types of salts:
1- Neutral salts (Monoacidic) (less water soluble.
2- Acidic salts (Diacidic) water soluble.
OHHO
OH
HO COOH
Quinic acid
• Both Quinine and Quinidine, Cinchonine and Cinchonidine are Diastereoisomers. Each pair differs in the stereochemistry at C-8 and C-9.
N
N
H3CO
HO
H H
N
N
H3CO
HO
H H
89
Quinine Quinidine
Properties:• Quinine is very slightly soluble in H2O, soluble in
ethanol, chloroform, ether, benzene and other organic solvents.
• Quinine is a diacidic base. It forms 2 types of sulfates:
– Quinine monosulfate (neutral and H2O insoluble).
– Quinine bisulfate (acidic and H2O soluble).
• Quinine (l-isomer) gives quinidine (d-isomer) among other products when warmed with KOH in amyl alcohol.
• Quinine is levorotatory, while quinidine is dextrorotatory.
Separation of the 4 AlkaloidsPowdered Bark
- Alk. CaO + NaOH + H2O- Reflux with benzene &- Filtration while hot
Benzene filtrate (Alkaloidal bases)
dilute H2SO4
Acidic aqueous layer(Alkaloidal bisulphates)
pH to 6.5 with Na2CO3
PrecipitateQuinine quinidine,
cinchonine & cinchonidine Hot water, Na2CO3
Quinine NaOH, ether
Aqueous layerEther layer
CinchonineQuinidine & Cinchonidine-HCl PH=7, Na, K Tart. salt- filter
Precipitate Filtrate
Cinchonidine tartrate Quinidine tartrate
Aqueous solution (Monosulphates)(monosulphate)
• Tests for identification1- Fluorescence test: Solution of the alkaloid in oxygenated acids (e.g H2SO4, HNO3 or
phosphoric acid) blue fluorescence (+ ve with quinine and quinidine).
2- Thalleoquine test: Aqueous solution of the alkaloidal salt + Br2 /H2O (few drops till the
appearance of yellow color) + NH4OH emerald green color
(+ ve with quinine and quinidine).
3- Rosequin test (Erythroquinine test):
Aqueous solution of the alkaloidal salt + dil HCl + Br2 /H2O (few drops till the appearance of yellow color) + CHCl3 + pot. Ferrocyanide + NH4OH
red color in the CHCl3 layer (+ ve with quinine and quinidine).
Uses:• Quinine is used mainly as anti-malarial in a dose
of 2g of quinine sulfate or other salt for 14 days.• Quinidine is used as a cardiac depressant
(anti-arrhythmic), particularly to inhibit auricular fibrillation in a dose of 0.6-1.6 g of quinidine sulfate daily.
• Cinchonine and cinchonidine are used as anti-inflammatory.
Isoquinoline Alkaloids1- Ipecacuanha Alkaloids
• Occurrence:Ipecac is the dried roots and rhizomes of Cephalis ipecacuanha (Brazilian ipecacuanha) or Cephalis acuminata (Cartagena or Panama ipecacuanha) Fam. Rubiaceae.
It contains several alkaloids (2 –2.5 %), mainly emetine (50- 70 % of total alkaloids), with cephaline and psychotrine.
N
MeO
RO
N
OMeOMe
HH
R = Me EmetineR = H Cephaline
N
MeO
HO
N
OMeOMe
H
Psychotrine
• Emetine: It is non phenolic and levorotatory.
It contains 2 basic nitrogens.
• Cephaline: It is phenolic and levorotatory.
It gives emetine on methylation with (CH3)2 SO4.
• Psychotrine: Occurs as yellow prisms.
It is phenolic and gives cephaline on reduction.
It gives emetine on reduction followed by methylation.
Isolation of Ipeca alkaloids:Powdered root and rhizome
- Ext. alcohol,Conc., lead acetate. - Filtration
Filtrate Residue Non-alkaloidal sub.
- Evap., dil. HCl & Filtration
Alkaloidal bases and salts
Filtrate (Alkaloidal HCl salts)
- Alk. with NaOH & Ext. with ether
Ether layerEmetine
(Phenolic alkaloids)- HCl (Alkaloidal salts)
- Alk. NH4OH Ext. ether
Aqueous layer Ether layer
(Psychotrine) (Cephaline)
Aq.alk. solution
Tests for identification of Ipeca alkaloids:
• Alkaloidal solution in HCl + Ca hypochlorite orange color.
• Emetine and cephaline + Froehd's reagent: dirty green color (the color with emetine fades by addition of HCl).
• Psychotrine + Froehd's reagent: pale green color.
• Cephaline and psychotrine + p-nitrodiazobenzene dye soluble in NaOH purple color.
• Psychotrine + conc. H2SO4 + HNO3 cherry red color.
• Emetine + Liebermann's reagent black color.
Uses:
• Emetine and cephaeline have antitumour and antiviral activity, but are too toxic for therapeutic use.
• Emetine and psychotrine are mainly used as emetic drugs.
• The crude drug is used as expectorant (due to its saponin content) .
• Emetine HCl and Dehydroemetine (DHE) have an antiamoebic effect, and are used for the treatment of amoebic dysentry and Fasciola.
• Ipeca alkaloids are diaphoretic, alone or in combination with opium (e.g. Dover's powder).
2- Curare Alkaloids• Occurrence:• Curare or South American arrow poison is the dried crude extract, obtained
mainly from Chondrodendron tomentosum (Menispermaceae) and certain Strychnos species, (Loganiaceae).
• Curare contains several alkaloids (4-7%), the most important is d-tubocurarine.
N
OH
MeO
N
H
H
Me
OMe
O
OH
O
Me
+Me
• Properties: d-tubocurarine (4ary alkaloid) is freely soluble in H2O. It is a phenolic
dextrorotatory alkaloid. It is a bis-benzyltetrahydroisoquinoline alkaloid.
• Tests: Saturated aqueous solution + FeCl3 faint green color green color. Solution of the alkaloidal HC1 + Na2CO 3 yellow brown precipitate.
• Uses: Tubocurarine chloride is mainly used by i.m. or i.v. routes as
skeletal muscle relaxant. It is used to control and reduce convulsions of strychnine
poisoning and of tetanus. It is used as a diagnostic aid in myasthenia gravis.