10 Years of Discovery & Innovation

• Highest resolution AFM with inverted optical microscope

• Simultaneous AFM and fl uorescence imaging

• Easy imaging in fl uids or ambient air

• Gentlest, non-destructive AFM imaging technique

• Effortless set-up with top-down scanner design

• Inverted optical view

• Compatible with MAC Mode, PicoTREC and other modes

B e n e f i t s

• Patented, rigid stage mounting

• Easy, Quick-slide sample loading mechanism

• Several sample handling stages available

• Sub-nanometer resolution

• Open-top viewing access and top illumination

• Flow-through liquid cell

• Temperature control available

O v e r v i e w

F e a t u r e s

Molecular Imaging’s BioScan system combines the power of a high-resolution atomic force microscope (AFM) with the direct optical viewing capability of an inverted optical microscope. The BioScan, from the leading developer of AFM systems for imaging in fl uids, offers unparalleled performance and ease of use. It extends the power of the atomic force microscope to studies of single molecules, polymers, cell membranes, whole cells, and much more. And, atomic force and optical or fl uorescence microscopy data can be obtained simultaneously.

BioScan’s patented mounting design incorporates a rigid structure that guarantees the low noise fl oor specifi cation required for obtain-ing the highest resolution images. With BioScan, sub-nanometer resolution is possible. And, the advanced design allows the AFM to sit on top of an inverted microscope and under the illumination pillar that results in better optical contrast for the images.

The Quick-slide™ sample loading mechanism and a fl exible sample handling stage makes sample preparation easy. The microscope is positioned on the Quick-slide assembly that lets the user change samples and/or solutions without effecting the alignment of the AFM or the optical microscope.

Figure 2: Figure 2: Image of Bioscan Image of Bioscan AFM topography of a red blood cell. 11µm x 11µm

Figure 3: Figure 3: Figure 3: Figure 3: Quick-slideQuick-slide for for easy sample loading.

Figure 1: Figure 1: Optical view of Optical view of the red blood cell sample with probe.

BioScan™

High-resolution AFM Imaging on an Inverted Optical Microscope

Molecular Imaging Corporation • 4666 S. Ash Avenue • Tempe, AZ 85282 USA

Tel +1.480.753.4311 • Fax +1.480.753.4312 • info@molec.com • www.molec.com

BioScan is a modular, multiple-user platform. The open architecture design permits easy access, manipulation, set-up and modification of samples while experiments are being performed. Easy access to the sample stage and familiar sample preparation techniques make BioScan ideal for life science and other applications that require intensive sample preparation.

Molecular Imaging’s unique modular design also makes it easy to change imaging techniques (for example, MAC to EC or AFM to STM) or to use the AFM microscope or the optical microscope as stand-alone systems. Other operating modes available include CSAFM.

• Array techniques• FRET• Single molecule fl uorescence• Dark fi eld microscopy• Solid phase synthesis

Applications

©Molecular Imaging Corporation, 2004All Trademarks mentioned in this document are trademarks or registered trademarks of their respective owners. All rights reserved. The information presented in this document does not form part of any quotation or contract and is believed to be accurate and reliable. All values are nominal. Molecular Imaging reserves the right to make changes without notice to improve design and supply customers with the best possible products. Printed in USA PF 004 BSCN 5-04-2500

Optimized for use with Molecular Imaging’s PicoPlus® or PicoLE™ AFM’s, BioScan allows researchers to take advantage of the many powerful features that are available only with Molecular Imaging’s Pico Family™ of products. MAC Mode®, the most gentle imaging mode available for any AFM platform, provides unbeatable performance in fl uids. Flow-through liquid cells and precise temperature control options allow users to image soft biological samples under controlled physiological conditions. Molecular Imaging’s proprietary sample plate stability further supports high-resolution AFM. And, the PicoTREC™ option delivers real-time simultaneous topography and recognition imaging.

• Life Science/Biology – – – DNA – – – Proteins – – – Cell biology• Polymer• Thin fi lms• Nano beads• Electrochemistry • Non-aqueous solvents

Imaging options

Industry Standard Compatibi l i ty

Figures 10 & 11: Sample plate with Petri dish (left) and cover slide with liquid cell (right).

Top I l lumination

Figures 8a & 8b: Bottom (left) and top (right) illumination image of pollens at 2400x shows signifi cantly improved contrast.

Figure 4a: Original CCD fi eld of view with DNA bundle in center.

Imaging with MAC Mode

Figure 5: Schematic setup of top-down MAC Mode.

Figure 6: CHO cells in buffer imaged with MAC Mode. 70µm x 70µm.

Figure 7: Ferritin molecules in water imaged with MAC Mode (raw data). 1µm x 1µm.

• Petri dish stages and microscope slides• Wide selection of inverted optical microscopes• Many camera options

Combed Combed λλ DNA DNA

Figure 4b: Corresponding AFM scan in-situ,30 nm x 45 nm.

Fluorescence Imaging

Multi-user Operation Complimentary Techniques

Magnetic coating

Solenoid

Figure 9: Figure 9: Assembly of the PicoPlus with inverted microscope, illumination pillar and CCD camera.

BioScan™High-resolution AFM Imaging on an Inverted Optical Microscope