PENGANTAR BIOTEKNOLOGI

BIOREMIDIATION

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATION TECHNIQUE

CASE STUDY

What is BIOREMEDIATION

The technology used to speed up the natural processes

of waste degradation and recycling

Use of naturally occurring microorganism such as

bacteria, fungi, and yeast to degrade pollutants or

hazardous substances in soil, water and air into non-

toxic or less toxic substances in order to return the

environment to its original natural condition

PLANTS (Phytoremediation)

ENZYME

INORGANICORGANIC

TRIPLE CORNERS PROSESS

POLLUTANS

ORGANISMENVIRONMENT

MICROORGANISMSOILWATERAIR Confining

Unit

Water table

Saline Water

Lateralintrusion of saline water

Ocean

Municipalwater well

Abandonedoil well

DeepAquifer

pond

Infiltration ofpesticides andfertilizers from

farmlands

Brine leakage from ruptured well casing

septic tank leakage

Fresh water

Accidental fuel spill

Municipal landfill

Leakage from hazardous waste site

Contaminated shallow

well

Leakingpetroleum

tank

ConfiningUnit

MICROORGANISM

1- Isolation of the microorganism

5- Determination of the biodegradation

efficiency

4- Optimization of the biodegradation

conditions

3- Identification of the microbial isolate

2- Purification of the obtained isolates

6- Identification of the biodegradation products.

7- Cell or enzyme immobilization.

8- Enzyme identification.

GMO APPLICATION FOR

BIOREMIDIATION

Deinococcus radiodurans

organisme paling radioresistant

dimodifikasi untuk dapat mencerna

toluene dan ionic mercury dari limbah

dg kandunga radioactive nuclear yang

tinggi

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATION TECHNIQUE

CASE STUDY

PHYTOREMEDIATION Phytoremediation is use of plants (HYPERACCUMULATOR

PLANT) for accumulation, removal or conversion of pollutants.

TUMBUHAN MENARIK ZAT KONTAMINAN, BERAKUMULASI DI DAUN

PENEMPELAN ZAT KONTAMINAN PADA AKAR

(Rhyzodegradation)PENGURAIAN ZAT KONTAMINAN OLEH AKTIVITAS MO

TRANSPIRASI ZAT KONTAMINAN DLM BENTUK MENJADI LARUTAN

TERURAI TDK BERBAHAYA

PENGURAIAN ZAT KONTAMINAN

HYPERACCUMULATOR PLANT

Can adsorb more than 10.000 ppm for Mn, Zn, Ni

> 1.000 ppm for Cu and Se

> 100 ppm for Cd, Cr, Pb and Co

METAL CONTAMNANTS IN THE SOIL

are ABSORBED by the roots (UPTAKE)

move in to shoot (TRANSLOCATION)

are STORED in the shoot (ACCUMULATION)

Alyssum murale

Nicotina tabbacum

Thiaspi caerulescens

Berkheya sp

FITOREMIDIATION BENEFIT

LOW COST OPERATIONAL

DESCRESE CONTAMINANT POLLUTANS

NATURALLY

CONTAMINATED PLANT CAN USED AS FUEL

MYCOREMEDIATION

USE FUNGGI AS DECONTAMINATION AREAL

USED FUNGAL MYCELIA

EX: DIESEL OIL CONTAMINATED AREA

INOCULATE WITH OYSTER FUNGI. CAN

CHANGE PAH (polycyclic aromatic

hydrocarbon) CO2 + H2O

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATION TECHNIQUE

CASE STUDY

IN SITU BIOREMEDIATION

SOIL BIOREMIDIATION (IN SITU PROCESSING)

BIOVENTING

Pemompaan udara dan

Nutrisi

AIR SPARGING

Pememompaan udara

untuk meningkatkan

aktifitas degradasi oleh

mikroba

SOIL BIOREMIDIATION (EX-SITU PROCESSING)

Tanah terkontaminasi diangkat ke dan diperlakukan di

permukaan

SOIL BIOREMIDIATION (EX-SITU PROCESSING)

SLURRY PHASE

menggunakan bioreaktor

SOIL BIOREMIDIATION (COMBINATION IN SITU & EX-SITU PROCESSING)

WATER AND GAS BIOREMIDIATION

Biofiltration is a process, in which,

microorganisms supported on inert

materials are used to degrade organic

pollutants for air, gas and water

bioremediation.

Types of biofilters:

1- Bioscrubbers.

2- Biotrickling filters.

3- Slow sand or carbon filters.

Bioscrubber filters

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATION TECHNIQUE

CASE STUDY

Case Study

Sebuah perusahaan POM BENSIN terbesar di Las Vegas, USA

sedang menhadapi masalah serius. Tangki yang ditanam dalam

tanah sedalam 10 meter mengalami kebocoran.

Jika Anda sebagai Konsultan, diminta

untuk menyelesaikan masalah

perusahaan POM BENSIN, Las Vegas,

USA tersebut. Apa yang akan anda

lakukan ?

1. THE EXXON VALDEZ OIL SPILS2. TRAGEDI MINAMATA3. KASUS LOVE CANAL (AMERIKA

SERIKAT)4. KASUS KABUT DIOXIN DI

SEVESO (ITALIA)5. PT. NEWMONT MINAHASA

RAYA PENCEMAR TELUK BUYAT

• JELASKAN KASUS PENCEMARAN TERSEBUT SERTA DAMPAK YANG DITIMBULKAN

• JIKA ANDA SEBAGAI KONSULTAN BIDANG LINGKUNGAN SOLUSI APAKAH YANG AKAN BERIKAN UNTUK MENGATASI MASALAH TERSEBUT (IDENTIFIKASI POLLUTAN, ENVIRONMENT DAN ORGANISM)