bioreactor education & training w.marner ccp13
DESCRIPTION
Technicians' Role in Growing the BioeconomyTRANSCRIPT
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Slide 1
Technicians Role in Growing the Bioeconomy PanelWesley D. Marner II, Ph.D.22 April 2013
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Slide 2
Benchtop(1-20L)
Pilot/Production(7-2,000L)
Applikon at a glance• Worldwide provider
of bioreactor equipment.
www.applikonbio.com
Slide 3
Bioprocessing has traditionally been at larger culture volumes
ManufacturingProcess Development and Scale UpUpstream Research
• Bioreactor operation• Seed culture manipulation• Harvest and downstream
purification• Equipment
• Data analysis• Experimental design• Regulatory requirements• cGMP protocols• Industrial safety
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Slide 4
Consider early development screening...
• Early stage "winners" can lock in the route of development work during scale up to production
10,000 1,000 150 25
“Winners”
≠Unfortunately,
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Slide 5
Trend toward greater bioprocessing examination, sooner.
ManufacturingProcess Development and Scale UpUpstream Research
# of Cultures Cost / Culture Process Information
Num
ber o
f Cul
ture
s
Cos
t p
er C
ult
ure
www.applikonbio.com
Slide 6
Trend toward greater bioprocessing examination, sooner.
ManufacturingProcess Development and Scale UpUpstream Research
# of Cultures Cost / Culture Process Information
Num
ber o
f Cul
ture
s
Cos
t p
er C
ult
ure
Acquiring process data sooner.
www.applikonbio.com
Slide 7
Trend toward greater bioprocessing examination, sooner.
ManufacturingProcess Development and Scale UpUpstream Research
# of Cultures Cost / Culture Process Information
Num
ber o
f Cul
ture
s
Cos
t p
er C
ult
ure
Reducing cost of early development.
Acquiring process data sooner.
www.applikonbio.com
Slide 8
Micro(200µl)
Benchtop(1-20L)
Pilot/Production(7-2,000L)
Mini(0.25-3L)
Applikon at a glance• Worldwide provider
of bioreactor equipment at all scales.
www.applikonbio.com
Slide 9
New technology for bioprocessing at small scales:
Sensor Shake Flasks
OD Readers
Sensor Dishes
RAMbio
Mini Bioreactors
Parallel Off-Gas
Well Plates
Process Optimization:Bioreactor Experiments
Process Definition:Well Plates,
Flask Characterization
Strain Construction:Tubes, Well Plates
Flasks, Shakers
Identification:Cloning, Fluorescence,
Well Plates, Flasks
Process Development ScreeningSelectionResearch
ClassicAutoclavables
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Slide 10
• Bioprocessing principles in upstream research
• Bioreactor startup and operation• Bioreactor control & typical parameters
• Culture modes (batch, fed-batch, continuous)• Culture kinetics (growth, nutrient uptake, product
formation)• Bioengineering basics (gas transfer, mixing)
• Understanding the abilities and limitations of small-scale tools
• Small bioreactors vs. large one• Artifacts of microtiter plates• Advantages/Disadvantages of scaling down
• Data analysis and statistics - and communicating these data
