biological molecules lipids proteinsnucleic acids carbohydrates

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Biological Molecules Lipids Proteins Nucleic Acids Carbohydrates

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Biological Molecules

Lipids

Proteins Nucleic Acids

Carbohydrates

Hydroxyl ( -OH )

Carbonyl (C=O)

O H

C O

Alcohols

Aldehydes, Ketones

USE A WATER BATH

• Plant starch turn to a blue-black color; • Glycogen (animal starch) turns red-brown; • Cellulose (the major component of plant cell

walls) turns violet-brown to red-brown.

Lugol’s Iodine

Liver cells

Plant cells

Potato

Lipids

Sudan III

Proteins

Biuret Reagent

The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and sodium hydroxide (NaOH). The NaOH is there to raise the pH of the solution ...

Nucleic Acids

• Qiagen DNAeasy kit. • Quantify our yields using a Nanodrop system.

Controls:• water• 1% glucose (a reducing sugar)• 1% egg albumin (a protein)• 1% soluble plant starch (a polysaccharide)• corn oil (a lipid)

Procedures and AssignmentI. TESTING FOR THE PRESENCE OF BIOLOGICAL COMPOUNDS IN FOOD

Use the food products you brought to this lab

Tests:I.A. BENEDICT’S TEST I.B. LUGOL’S I.C. SUDAN III TESTI.D. BIURET TEST

Figure 4.Summary of DNA extraction protocol.II.A. DNA Extraction and Purification

II. DNA Extraction, Purification and Quantification

Nanodrop spectrometer used to measure DNA concentrations

II.B. DNA Quantitation

III. CLEAN UP

• Clean your test tubes with detergent and return them (inverted to dry) to their test tube racks.

• Return all re-usable supplies to their original location.

• Disinfect your workstation.