biochemistry the chemistry of life. basic chemistry atom –simplest unit of matter –made up of...
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Biochemistry
The Chemistry of Life
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Basic Chemistry
• Atom– Simplest unit of matter– Made up of three different “subatomic”
particles
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Subatomic Particles
• Protons– Have a positive charge (+)– Have mass– Located in nucleus
• Neutrons– Are neutral, have no charge (0)– Have mass– Located in nucleus
• Electrons– Have a negative charge (-)– Almost no mass– Located in “energy levels” outside of nucleus
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• Atomic Number: – Tells you the number of protons an atom has,
and also the # of electrons
Atoms are neutral
# Protons (+) = # Electrons (-)
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• Atomic Mass:– Add together the number of protons and
neutrons in nucleus of atom– Electrons hardly contribute any mass
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• Isotopes:– Atoms of one type of element with different
atomic masses
– Same # of protons– Different # neutrons
– Changes the mass only, the properties are the same
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• Radioactive Isotopes:– The nucleus of some isotopes is unstable– The atom with emit radiation – Radiation can be measured with tools
• ex. Geiger counter
– These isotopes can be used as useful tools in science
– Ex: • Carbon-14 dating• Radiation treatment for cancer
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• Elements– Substance made of only one type of atom– Each element has a unique atomic #
– Elements most commonly found in living things
• Carbon (C) • Hydrogen (H)• Oxygen (O)• Nitrogen (N)
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• Compounds– Two or more elements chemically combined – Atoms held together by bonds.– Once bonds form, compounds will have new,
and different properties
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– Organic Compounds• Contains carbon and hydrogen (and often O, N)
• Ex: Glucose (C6H12O6), carbohydrate, lipids, proteins
– Inorganic Compounds• Doesn’t contain carbon and hydrogen together
• Ex: H2O, CO2, NH3, NaCl
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• Why do atoms form compounds?
– Bonds that form between atoms give atoms a stable outer electron level.
– Called a “stable octet” of valence electrons (8)
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Types of Chemical Bonds
• Ionic Bonds:– One or more electrons are transferred from
one atom to another.– Neutral atoms become positive & negative ions– Forms salts
• (Ex: NaCl)
Ionic bond = attraction between (+) and (-) ions
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• Covalent Bonds:– Electrons are shared between atoms– Each bond represents a shared pair of electrons– Can form single, double or even triple bonds
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Covalent bonds form molecules– Subscripts in the formula tell # of each atom
• Ex: H2O, NH3, CO2, CH4
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Ionic Bonds:
Transfer electrons
Covalent Bonds:
Share Electrons
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Properties of Water
• Water is necessary for life
• Contains covalent bonds
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Water Molecules: H2O
(look like Mickey Mouse)
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• Water is a “Polar” molecule (like a magnet)
• The bonds are “polar”– Oxygen attracts the electrons
more than Hydrogen
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• Hydrogen Bonding: bonding between the (+) H of one molecule and the (-) end of another molecule.
–Makes water good at sticking to itself and other substances–Makes water good dissolver
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• Cohesion: water sticks to itself– Ex:
• Water forms “beads” on smooth surface• Surface Tension allows insect to “skate”
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• Adhesion: water sticks to other substances
– Ex: Capillary action: water molecules rise up small tubes
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• Heat Capacity:– Water has a relatively high heat capacity– Ex:
• Lakes and oceans can absorb a lot of heat from sun without a drastic temperature change
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• Water as a Solvent:– Dissolves most ionic and covalent substances– “Universal Solvent” = many things can
dissolve in it.
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– Solute: substance being dissolved– Solvent: substance in which solute dissolves– Solution: evenly disbursed mixture
– Suspensions: material in the water but just suspended not dissolved (ex: blood cells in blood)
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pH of Solutions• pH Scale: way to measure concentration
of H+ ions in solution– Ranges from 0 to 14– Pure water is neutral pH = 7
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• Litmus Paper:– Used to test pH of a solution
• Red = acidic• Blue = basic
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• Acids: Form H+ ions
–pH is <7
–Ex: HCl (stomach acid), lemon juice
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• Bases: Produces OH- (hydroxide ions)– Also called “alkaline”
– pH >7
– Ex: Lye (NaOH) used as drain cleaner
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• Buffers:– Maintain pH at a certain level – Usually between 6.5 – 7.5 (close to neutral)– Helps to maintain homeostasis in organism
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Major Types of Chemical Reactions
• Dehydration Synthesis: (Condensation)
– Chemically combine two smaller molecules– Water is removed
– “Dehydrate” = remove water– “Synthesis” = to make
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• Hydrolysis:
– Break apart large molecule into smaller pieces
– Water is added
– “Hydro” = water “Lysis” = to break
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• Polymerization:– Create a large molecule (polymer)– Join up smaller “monomer” units– Often a dehydration synthesis reaction
Ex: Join amino acids (monomer) to make protein (polymer)
Join glucose (monosaccharide) to make starch (polysaccharide)
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Organic Compounds
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Carbohydrates
• Sugars and starches• FUNCTION:
– Used as an energy source– Energy released during cellular respiration
• Made of carbon, hydrogen and oxygen
Ex:
C6H12O6, C12H22O11
• Ratio of H of O is always 2:1
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• Basic Structure:– “Ring” made of 5 carbons and 1 oxygen– Rings can join up by dehydration synthesis
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3 Types of Carbohydrates
• Monosaccharide's: 1 sugar ring
• Disaccharides: 2 sugar rings
• Polysaccharides: many sugar rings
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• Monosaccharide's: (Simple sugars)– All have formula C6H12O6
– Single ring structure– End in “-ose”– Ex: glucose, fructose, galactose
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• Disaccharides: double sugars– All have formula C12H22O11
– End in “-ose”– Ex: sucrose, lactose, maltose
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• Polysaccharides: 3 or more sugar units
• Ex: – Starch (energy storage in plants)– Glycogen (how animals store sugar in liver)– Cellulose (plant cell walls)– Chitin (insect exoskeletons)
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• Simple sugars form into complex sugars by dehydration synthesis (condensation).
• Combining molecules by removing water
Monosac. + Monosac. Disac. + Water
C6H12O6 + C6H12O6 C12H22O11 + H2O
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and H2O
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• Complex sugars are broken down into simple sugars by hydrolysis.
• Breaking down molecules by adding water
• Also called chemical digestion
Disac.+ Water Monosac. + Monosac.
C12H22O11 + H2O C6H12O6 + C6H12O6
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Dehydration Synthesis and Hydrolysis are
OPPOSITE Reactions
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**Chemical reactions are often enzyme mediated.
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Lipids
• Fats, oils and waxes• FUNCTION:
– Long term energy storage– Insulation – Protective coating around cells– Cell membranes
• Made of carbon, hydrogen, and oxygen• Ratio of H to O is not 2:1
– Ex: C15H26O6 (fewer oxygens)
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• Animals store energy mostly as fats
• Plants store energy mostly as oils
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Lipids
• Made up of:– 1 glycerol molecule– 3 long fatty acid chains
– Looks like a giant letter “E”– Also called a “triglyceride”
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GLYCEROL
FATTY ACID CHAIN
Note: 3 “OH” groups
Note: CARBOXYL GROUP: “COOH” group at end of molecule
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Basic Lipid Structure
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• Lipids are:– formed by dehydration synthesis– broken down by hydrolysis
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Saturated vs. Unsaturated Fats
– Saturated: • all single bonds between carbon atoms
– Unsaturated:• One or more double bonds between carbon atoms• Makes fatty acid more bent
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Proteins
• Protein Structure:– Made of carbon, hydrogen, oxygen and
NITROGEN
– Large complex polymer molecules that can have a large range of sizes, shapes and properties
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Proteins• FUNCTIONS:
– Enzymes: speed up chemical reactions
– Hormones: chemical messengers
– Antibodies: defend against disease
– Hemoglobin: binds oxygen to red blood cells
– Cell growth and repair
– Cell Membrane Channels
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• Amino Acid: basic building block of proteins
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Parts of an Amino Acid
• Carboxylic Acid Group
• Amino Group
• “R”-Group (varies depending on amino acid)
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Examples of Different Amino Acids
• There are 20 different amino acids
• All have different “R” groups
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Peptide Bond: bond between amino acids
Dipeptide: two amino acids joined.
Polypeptide: many amino acids joined– Proteins are polypeptides
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Forming a Peptide Bond
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and H2O
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+ H2O
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• Amino acids join up to form proteins at ribosome• The function of the protein comes from it’s
specific sequence of amino acids and the shape the protein forms
• The “code” for the specific sequence of amino acids comes from DNA
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Nucleic Acids
• Large complex macromolecule
• Stores information in “code”
• Composed of carbon, hydrogen, oxygen, nitrogen and phosphorus
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Structure of Nucleic Acids:
•Made of chains of nucleotides
Nucleotide: (made of 3 components)– Phosphate Group– Sugar– Nitrogenous Base
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Two Types of Nucleic Acids
• DNA: (Deoxyribonucleic Acid)– Contain genetic information– Found in nucleus– Divides when cell divides
• RNA: (Ribonucleic Acid)– Copies code from DNA– Takes code to ribosomes for protein synthesis– Found in nucleus and cytoplasm
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Differences Between DNA & RNA
– DNA: • Double strand of nucleotides• Sugar is deoxyribose• Nitrogenous Bases
– Adenine, Thymine, Cytosine, Guanine
– RNA: • Single strand of nucleotides• Sugar is ribose• Nitrogenous bases
– Adenine, Uracil, Cytosine, Guanine
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