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CURRICULUM VITAE

NAMA : PARAMASARI DIRGAHAYU., dr., PhD

Lahir : Purwokerto, 21 April 1966

PENDIDIKAN

S1 : FK UNS Surakarta (1992)

S2-S3 : Tottori University Japan (1999-2003)

Post Doctoral : Tottori University Japan (2003-2004)

Field of Interest : Immuno-ParasitologyMolecular Biology and Biotechnology

PEKERJAAN:

Kepala Instalasi Parasitologi Klinik dan Mikologi RSDM (2007)

Kepala Pusat Bioteknologi dan Biodiversitas LPPM UNS (2005) Wa Ka Lab Parasitologi-Mikologi FK UNS (2007)

Koordinator International Office UNS (2009)

Sekretaris Lab Biomedik Terpadu FK UNS (2006)

Assesor BAN PT DIKTI (2007)

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Memahami peran ilmu Biologi molekuler di bidang

kedokteran dan kesehatan.

Mengerti dasar/ jargon ilmiah di bidang biologi molekuler

Memahami prinsip-prinsip dasar biologi molekuler dan

Bioteknologi dibidang kedokteran sehingga mampu

menyusun experimental design/memahami journal

penelitian internasional berbasis molekuler.

Pendahuluan

Kompetensi yang harus dicapai:

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SYL BUS M T KULI H BIOLOGI MOLEKULERKuliah Topik Pengampu

1 Filosofi Biologi Molekuler dan

Perannya di Bidang Kesehatan-1

(Prof . DR. dr. Ambar

Moedigdo., SpPA(K)

2 Filosofi Biologi Molekuler dan

Perannya di Bidang Kesehatan-1

(Prof . DR. dr. Ambar

Moedigdo., SpPA(K)

3 Struktur dan fungsi Sel, virus, DNA,

RNA dan Protein

(Afiono Agung S, dr., PhD)

4 Virologi Molekular dan aplikasi di bidangkedokteran Afiono Agung P.,dr.,PhD

5 Sel structure and Gene Expression Paramasari Dirgahayu

6 Tehnik Biologi Molekuler dan Aplikasinya di

bidang kedokteran-1

Diagnostik dan Terapi Molekuler

Paramasari Dirgahayu,Paramasari Dirgahayu, dr.,

PhD

7

8 Jurnal reading-1

Paramasari Dirgahayu, dr.,

PhD + (Afiono Agung S, dr.,

PhD)

9 Jurnal reading-2 (Paramasari Dirgahayu, dr.,

PhD + (Afiono Agung S, dr.,PhD)

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Fungsi Ilmu Bioteknologi

1. Diagnosa penyakit 2. Prevensi penyakit herediter

3. Terapi molekuler (stem cell terapi,

DNA terapi, dll)4. Riset/Klarifikasi host-Agent relation

5. Pembuatan vaksin

6. Forensik molekular

7. Epidemiologi molekuler

8. dll

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TEKNIK BIOLOGI MOLEKULER

(BIOTEKNOLOGI)

Digunakan dalam identifikasi ekspresi genetik/protein secarakuantitatif/kualitatif

Terpenting:

Preparasi sample : dari Invivo/In Vitro experiment

berupa DNA/RNA/Protein?

Tentukan metode identifikasi sample

1. Isolasi DNA

2. PCR

3. Western Blot

4. Northern Blot

5. Southern blot

6. Immunohistokimia

7. ELISA

8. Sequencing

9. Cloning

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Overview

BODY

ORGANS

CELLS

KROMOSOMS

DNA

GENE

PHENOTYPE

GENOTYPENUKLEUS

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What Is a Cell?

Cell Structurecomposition

(molecules)organization

Basic Unit of Life

metabolism

replication

cells acquire nutrients and convertthem to energy and self (growth)

all cells come from pre-existing cells

(reproduction)Laboratorium BIOMEDIK TERPADU Fakultas Kedokteran UNS

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Eukaryote vs Prokaryote

Eukaryotic cells have

membrane-bound

compartments with

specialized functions.

Mitochondria

Plasma

Membrane

Nucleus

Lysosome

ER

Golgi

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DNA

Deoxyribonucleic Acid

Double helix

Carries genetic information

Located in the nucleus

The monomer is a nucleotide

A phosphate

A ribose sugar A nitrogenous base

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What are the bases in DNA

A adenine

T thymine

C cytosine

G

guanine

Base pair rules

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Where is DNA located in the Cell?

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HUMAN CHROMOSOMES

(KARYOTYPE)

THE chromosmes visualizedas they originally spilled from

the lysed cells

THE chromosmes artifiallyline up in their numerical

order

CELLS

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Human Karyotype

22 pairs of autosomes

size bands

sex chromosomes females: X/X

males: X/Y diploid (2n)

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Chromosomes

genomic DNA is very longhumans, 6 x 109bp

~1.8 meters total length

total length reduced via

associates with proteins individual DNA molecules

packaged into chromosomes

each chromosome represents

one linear DNA molecule

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Where are the genes located?

Genes are located on

the chromosomes.

Every species has a

different number ofchromosomes.

There are two types of

chromosomes:

autosomes and sexchromosomes

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Promoter regionTATA boxCAAT box (in mammals)

GC box (GGGCGGG)

Initiation codon Stop codon

Polyadenylationsignal

AATAAExon 1 Exon 2 Exon 3 Exon 4

Intron 1 Intron 2 Intron 3

5 3

Eukaryote

Gene structure

Initiation codon Stop codon

Promoter region

Shine-Dalgarno box (AGGAGG)Pribnow box (TATAAT)-35 site (TTGACA) Prokaryote

5 3

ORF

Terminator

Terminator

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GENOME

App. 3200 KM (2000 MILES)

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Genes

locus = physical location on a chromosome

gene = unit of information (~protein)

}}

5'ATGAAATTTTTAATACATCAATTAAACAATGATTATATAAAAAATGATATA

AGATATAAATCAAATGAAGAGAAATATATGAGTAAAGTAGACTTCCTAGAAACA

ATGAAAAAAGATATATCAGAACTGTACGAAGAATATGGAACGAATAATGAATCGAACACTTCGTATAACATAACGATGGATTTTGTAAACAATTTTGATAGCAGAGAT

GGGTCATATATAACTGATATAAGCAGAGAAGAGTTGATGTTAAAACAACATAAT

ATAATGAGATTGCAAGATGAACAACTTGAATTTCTTGAAGGAACAACACAAAAT

TTAAAAAGTATTAGCTATAATATAAATAACGAAATACAAGTACATAATGAAATA

TTAGATGATATAGATAGAGATATGGATGAAACAAGTGATTTATTGGATAGAAAT

AGAAACATTTTTACCAGAATTACTAACAGTACGAGTAATTATTATTTGTACATG

TTAATTTGTTTATTAACAGTTACACTTTTCTTTTTGATCATAATACTTTAG-3'

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ekuens

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Cells Structure - animation

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Gene expression is theactivation of a gene that

results in a protein.

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GENE

EXPRESSION

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Summary

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Transcription 1

(making a mRNA copy of DNA)

The part of the DNA molecule (the gene) that the cell wants theinformation from to make a protein unwinds to expose the bases.

Free mRNA nucleotides in the nucleus base pair with one strand of

the unwound DNA molecule.

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Transcription 2

The mRNA copy is made with the help of RNA polymerase. This enzyme joins

up the mRNA nucleotides to make a mRNA strand.

This mRNA strand is a complementary copy of the DNA (gene)

The mRNA molecule leaves the nucleus via a nuclear pore into the cytoplasm

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tRNA

pick up their specific amino acids from

the cytoplasm

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Transcription- animation

T l ti

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Translation

RNA

Single stranded Does not contain thymine but

has uracil instead.

tRNA carries 3 base pair codefor specific amino acid.

Amino acids composepolypeptid chains.

One or more polypeptidechains compose a protein

proteins provide the

blueprints for ourcharacteristics andfunctions.

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In Prokaryotes there are three (3) regulatory elements thatcontrol gene expression.

1. Structural genes genes that code for a specificpolypeptide (protein).

2. Promoter DNA segment that recognizes RNA

polymerase.3. Operator element that serves as a binding site for an

inhibitor protein that blocks transcription.

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Eukaryote

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Translation - animation

A ribosome

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A ribosome

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Translation - outline

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Translation.

mRNA used to make polypeptide chain

(protein)

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1.

First the mRNA attaches itself to a ribosome (to the small subunit).

Six bases of the mRNA are exposed.A complementary tRNA molecule with its attached amino acid (methionine) base pairsvia its anticodon UAC with the AUG on the mRNA in the first position P.

Another tRNA base pairs with the other three mRNA bases in the ribosome at positionA.

The enzyme peptidyl transferase forms a peptide bond between the two amino acids.

The first tRNA (without its amino acid) leaves the ribosome.

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Translation 2

The ribosome moves along the mRNA to the next codon (three bases).

The second tRNA molecule moves into position P.

Another tRNA molecule pairs with the mRNA in position A bringing its amino acid.

A growing polypeptide is formed in this way until a stop codon is reached.

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End of Translation

A stop codon on the mRNA is reached and this signals the ribosome to leave the

mRNA. A newly synthesised protein is now complete!

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Translation continued

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Translation continued

4 A second tRNA molecule binds to theribosome

5 The amino acid from the first tRNA isconnected to the amino acid of thesecond tRNA with the assistance ofthe ribosome

6 This process continues until the stop

codon on the mRNA is reached

7 At the stop codon, a chemicalreaction frees the polypeptide chain

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Protein synthesis - animation

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HOW TO EXAMINEEXPRESSIONS OF THE GENES