BiEau® Actif Brown Algae

16905 BiEau® Actif Brown AlgaeAntioxidant + Wrinkle Reduction + Nourishing

Product Code: 16905

INCI Name: Laminaria Japonica Juice

INCI Status: Proposed

Suggested Use Levels: 1.0 – 10.0%

Suggested Applications: Antioxidant, Reduces Wrinkles

Solubility: Water Soluble

• Laminaria Japonica or Brown Algae - an abundant source of nutrients including fucoidan

• Sulfated polysaccharides such as fucoidan typically involved in enhancing antioxidant capabilities and visibly reducing the appearance of fine lines and wrinkles

• Plants naturally utilize and bind water in their cells as a mechanism of survival • Cellular water is the living energy for plant survival

• This cellular water or plant essence offers the ability to harness a nutritional, isotonic solution capable of nourishing the skin and hair in personal care applications

• From cultured algal and mushroom cells to reanimated agricultural waste, Active Concepts maximizes raw material sourcing and creation for minimal environmental impact with the development of our BiEau® Actif botanical waters line

16905 BiEau® Actif Brown AlgaeBackground

• Skin is the largest organ of the body and is composed of approximately 60% water

• As a protective barrier, the skin is our best defense against external aggressors

• Skin’s permeability can be influenced by a variety of environmental attributes -pollution particles, cosmetic actives, temperature fluctuations, and water exchange

• A delicate balance exists between water introduced onto our skin, using cosmetics, cleansers etc, and the water present in our skin and cells.

• Traditionally water used in the creation of cosmetic ingredients, typically lacks minerals and natural components that our skin craves

• Isotonic solutions offer an environment most beneficial and least disruptive to skin

16905 BiEau® Actif Brown AlgaeWater is Life

• Isotonic solution – Equilibrium

• ‘Iso’ in Latin means equal

• Isotonic solutions have the same solute concentration on the inside of the cell as the outside of the cell

• Balanced condition between cells and environment

• Isotonic materials maintain the size, shape, and integrity of skin cells

• Hypertonic solutions cause cells to shrivel (dry out) while hypotonic solutions cause cells to swell (burst)

16905 BiEau® Actif Brown AlgaeSolution Impacts on Cells

• Nutrient transport, structure, growth, and even chemical processes such as photosynthesis rely on water functionality

• Algae embodies the concept of marine sourced sustainability promoting utilization in various industries as food, biofuels, filtering materials, soil fertilizer, pharmaceuticals and laboratory growth media

• Algae absorbs water and nutrients in their cells, directly from the surrounding water - internal cellular water contains an abundance of biologically active components

• Nutrient-rich water of Laminaria Japonica offers a cellular water capable of providing antioxidant protection

• Fucoidan naturally present –protects against oxidative stress

16905 BiEau® Actif Brown AlgaeCellular Water – Living Energy for Plant Survival

• We isolated the nutrient rich water from Laminaria Japonica to create BiEau® Actif Brown Algae, a vital botanical essence capable of offering antioxidant protection

• BiEau® Actif Brown Algae utilizes a novel approach to eco-conscious cosmetic active development while capitalizing on the presence of fucoidan to promote an overall younger appearance

• BiEau® Actif Brown Algae can be used in a variety of cosmetic and personal care formulations aimed to reduce the appearance of wrinkles and provide antioxidant capabilities while encouraging an isotonic environment beneficial for optimal health of the skin and hair

16905 BiEau® Actif Brown AlgaeBenefits

Figure 1. Average feature counts comparing experimental and control values at each time point.

16905 BiEau® Actif Brown AlgaeVISIA® Complexion Analysis

Protocol

• An in-vivo VISIA Complexion Analysis study was conducted using 16 M/F participants between the ages of 23 -37

• Each participant was instructed to apply 2.0 mg of lotion to their entire face twice a day for a four week period. Participants were instructed to continue their usual skin care routine and to apply the lotion once their everyday skin care routine is finished

• Half of the participant population used 5% BiEau® Actif Brown Algae a Cetaphil Daily Facial Moisturizer for all skin types, while the other half used the Cetaphil Daily Facial Moisturizer alone as a control

• Feature counts provide a count of the number of discrete instances of the feature being evaluated

• BiEau® Actif Brown Algae in base lotion displayed a reduced number of facial wrinkles when compared to the control

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Experimental (5.0% BiEau® Actif Brown Algae in Base Lotion) Control (Base Lotion)

16905 BiEau® Actif Brown AlgaeVISIA® Complexion Analysis

Protocol

• Photographic assessments were performed using the VISIA Complexion Analysis System (Canfield Scientific., Fairfield, NJ, USA). The VISIA System, with a configurable head support, ensured consistent positioning of each subject’s head

• The subjects cleaned their skin with a gentle facial wipe (Daily Facial Towelettes–Paraben Free Formula by Kirkland Signature) before the image was obtained. The photographic images were captured with standard, cross-polarized, parallel polarized, and ultraviolet light. Images were taken for each subject to quantify the feature counts for wrinkles

Protocol

• Solutions of BiEau® Actif Brown Algae and Trolox® were prepared in 10% DMSO, 75nM potassium phosphate buffer

• Materials were prepared at three dilutions• For the ORAC assay – test material and Trolox®

combined with fluorescein and incubated• Fluorescent measurements were taken every 2

minutes for 2 hours• ORAC values of samples calculated• BiEau® Actif Brown Algae is capable of providing

antioxidant benefits

Figure 4. Antioxidant capacity of BiEau® Actif Brown Algae

16905 BiEau® Actif Brown AlgaeAntioxidant ORAC Assay

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16905 BiEau® Actif Brown AlgaeAntioxidant + Wrinkle Reduction + Nourishing

Product Code: 16905

INCI Name: Laminaria Japonica Juice

INCI Status: Proposed

Suggested Use Levels: 1.0 – 10.0%

Suggested Applications: Antioxidant, Reduces Wrinkles

Solubility: Water Soluble

BiEau® Actif Brown AlgaeActive Concepts, LLCLincolnton, NC. USAwww. activeconceptsllc.com Office: +1 704 276 7100info@activeconceptsllc.com