bhoonaga satva bhasma a potent and less toxic alternative to tamra bhasma

BHOONAGA SATVA BHASMA : A POTENT AND LESS TOXIC

ALTERNATIVE TO TAMRA BHASMA

Guided By: Presented By:

DR.R.S.HIREMATH MD DR. VIKAS SAROCH

Asst. Professor II P.G. Scholar

Dr. (Mrs.) P.P.Dindore M.D.

Dr. Manojkumar S.Patil M.D.

P. G. Department of Rasashastra

K L E U’s

Shri. B.M.K.Ayurved Mahavidyalaya,

Shahapur, Belgaum-03

INTRODUCTION • Mineral origin of material contains many physical ,

chemical impurities.• Purification (Shodhana) and reduction to bhasma

(Marana) form, is lengthy, tedious procedure in some cases.

• In Rasagranthas we have reference about Bhunagasatwabhasma.

• Explained as substitute to Tamrabhasma which is of animal origin ( which requires comparatively less duration).

Monday,November 02,2009

2BHOONAGA SATVA BHASMA:A POTENT AND LESS TOXIC ALTERNATIVE TO TAMRA BHASMA

EXPERIMENTAL STUDY

(Acute toxicity Guideline No.423 ANNEX 2d)

• DIVIDED IN TWO PHASES

• LD- 50 dose estimation of Bhunaga SatwaBhasma and Tamra Bhasma.

• Comparitive Toxicity study of Bhunaga SatwaBhasma & Tamra Bhasma(Market Sample).



BHASMA PARIKSHA• Bhunagasatwa Bhasma passed all Bhasma

Pariksha including Amla, Nirutha, Apunarbhava Pariksha.

• Selection of Tamra Bhasma from market Sample A, B & C those Bhasma pariksha were carried out.

• Sample C passed all Bhasma Pariksha including Amla pariksha. This sample was taken for Toxicity study.



TOXICITY STUDY• No standard parameters for dose administration of

Bhunagasatwa Bhasma.

• LD 50 estimation of Bhunagasatwa Bhasma & Tamra Bhasma.

• Acute toxicity for 14 days.

• As per OECD guidelines of CPCSEA forum.

• Tamra Bhasma group shows mortality 2 animals out of 3 and Bhunagasatwa Bhasma no mortality.



LD50 Dose Estimation of Bhunaga

SatwaBhasma

• Bhunaga SatwaBhasma 2000 mg/kg

No Animal weight(g)

Dose(ml) Observation of toxicity reactions

Onset of time

Recovery period

Toxicity signs

1 24 0.46 -- -- N

2 22 0.44 -- -- N

3 22 0.44-- -- N



Animal no & weight (g)

Dosemg/kg

Onset of time

Recovery period

Toxicity signs observed30 min 1hr 2hr 3hr 4hr 24hr

1 242* 223* 22

200020002000

15 min30 min30 min

4hrs----

c, rd, nc, rd, nc, rd, n

rd, nc, rd, n, t

c, rd, n

rd, n--

c,rd,n,s,d

rd, n--

c,rd,n,s,d

rd, n--

c,rd,n,s,d

N----

1* 302 283* 25

100010001000

30 min40 min30 min

--2hrs

--

rd, nn

n, rd

c, rd, nnn

c, rd,n,sN

n, s

--N

n, s

--N

n, s

--N--

1 302 283 25

500500500

NNN

NNN

NNN

NNN

NNN

NNN

1 302 263 25

300300300

NNN

NNN

NNN

NNN

NNN

NNN

LD50 dose estimation of Tamra Bhasma (Market Sample)

*animals died during study; c-catatonia; d-diarrhea; n-narcosis; N-normal; rd-respiratory depression; s-salivation


7BHOONAGA SATVA BHASMA:A POTENT AND LESS

TOXIC ALTERNATIVE TO TAMRA BHASMA

Group Animal no & weight

(g)

Doseml/kg

Onset of time

Recovery period

Toxicity signs observed

30 min

1hr

2hr

3hr

4hr

24hr

6th day

7th

day14th day

Control 1 25 water (q.s.)

N N N N N N N N N N N

2 28

3 30

Bhunaga

Satwa Bhasma

1 28 0.56 N N N N N N N N N N N

2 36 0.70 N N N N N N N N N N N

3 26 0.52 N N N N N N N N N N N

Tamra Bhasma (market sample)

1

30 0.15 N N N N N N N N N N N

2 26 0.13 N N N N N N N N N N N

3 25 0.12 N N N N N N N N N N N

Acute oral toxicity record sheetSpecies: Mice (Swiss albino) Sex: Female Observation time: 24 hrs ,Duration: Upto 14 days

Dose: Test drug (BSB) – 200mg/kg Standard drug (TB) – 100 mg/kg

Quantitative analysis of and Bhunagasatwa Bhasma & Tamra Bhasma

Sl.no Test BhunagasatwaBhasma TamraBhasma

1 Copper 11.35% 63.84%

2 Borax 68.88% --

3 Sodium 10. 94 % --

4 Sulphur Nil 2.76%

5 Mercury -- ---

6 Iron --

5.34%



ACUTE ORAL TOXICITY

• LD 50 Determination of Tamra Bhasma was done on 1000 mg/kg b.w. where as for Bhunagasatwa Bhasma it is safe at 2000mg/kg (higher dose) and can be considered as a safe dose.

• Hematology & Biochemical parameters -The Hb % of both groups are increased.

• S.G.P.T. S.G.O.T. & serum Alkaline phosphate in Tamra Bhasma group was more increased compared to that of Bhunagasatwa Bhasma group.

It shows some obstructive pathology causing liver damage.

• Histopathology – HPR shows mild congestion in Bhunagasatwa Bhasma where

as in Tamra Bhasma shows moderate congestion.



CONCLUSION

• LD50 Dose estimation of Tamra Bhasma was estimated at 1000mg/kg and Bhunagasatwa Bhasma was safe at higher dose 2000mg/kg.

• Tamra Bhasma shows more significant Hepato toxicity where as Bhunagasatwa Bhasma shows minimum toxicity.

• Bhunagasatwa Bhasma can be used as substitute for Tamra Bhasma.



REFERENCES

• “Preparation and Physico-chemical analysis of Bhunagasatwa Bhasma

and its comparative toxicity study with Tamra Bhasma (Market

Sample)” By Dr. Manojkumar S.Patil

• Ayurved Prakasha Edition-1999,Pp:419

• Ayurved Prakasha,Edition-1999,Pp:418.

• Rasatarangini,11th Edition- 1979,Pp : 433.

• Fundamentals of Experimental Pharmacology, Scientific Book

Agency, Calcutta, 2nd Edition, Pp :156

• Committee for the Purpose of Control and Supervision of

Experiments on Animals (CPCSEA ), Govt. of India,(Rev.)- 2000.



bhoonaga satva bhasma a potent and less toxic alternative to tamra bhasma

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