bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb ......by frank gehry, and the vitrahaus,...

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JOURNAL OF BACTERIOLOGY, May, 1965 Copyright © 1965 American Society for Microbiology Vol. 89, No. 5 Printed in U.S.A. Location of Chlorophyll in Rhodospirillum rubrum STANLEY C. HOLT' AND ALLEN G. MARR Department of Bacteriology, University of California, Davis, California Received for publication 18 January 1965 ABSTRACT HOLT, STANLEY C. (University of California, Davis), AND ALLEN G. MARR. Location of chlorophyll in Rhodospirillum rubrum. J. Bacteriol. 89:1402-1412. 1965.-If cells of Rhodospirillum rubrum are broken by sonic and ballistic disruption, the chlorophyll is not found in discrete cytoplasmic structures, but is located in a more extensive struc- ture of the cell, the intracytoplasmic membrane. Direct electron microscopy of sonically disrupted cells of R. rubrum and stereo-electron microscopy of osmotically shocked cells reveal the presence of a tubular network of internal membranes originating from the periphery of the cell. Schachman, Pardee, and Stanier (1952) found that the photosynthetic pigments released from Rhodospirillum rubrum by sonic treatment and abrasion were sedimentable in the ultracentrifuge. A fraction was purified by differential centrifuga- tion; this fraction contained particles which were 600 to 1,000 A in diameter, had a sedimentation constant of 190S, and contained the entire pig- ment system of the cells. These particles were named chromatophores. Vatter and Wolfe (1958) initiated the study of the ultrastructure of the photosynthetic bacteria by examining thin sections of R. rubrum, Rhodo- pseudomonas spheroides, and Chromatium. The cy- toplasm of cells grown photosynthetically con- tained circular profiles approximately 500 to 1,000 A in diameter surrounding regions of low electron density. Cells grown heterotrophically did not contain these structures. Vatter and Wolfe (1958) concluded that these circular profiles were sec- tions of spherical particles identical with the chromatophores as isolated by Schachman and co-workers (1952). Tuttle and Gest (1959) found that all of the pigment in lysates of R. rubrum prepared by os- motic shock of protoplasts was sedimented at low centrifugal force, whereas chromatophores iso- lated after mechanical disruption required much larger centrifugal forces for sedimentation. Marr (1960) found that osmotic shock of R. rubrum released most of the nucleic acid but only 8% of the chlorophyll. These results could be explained either by the size of the chromatophores relative 1 Present address: Department of Microbiology, Dartmouth Medical School, Hanover, N.H. to the size of the opening in the cell produced by osmotic shock or by the location of the photosyn- thetic apparatus in a membranous continuum. Marr (1960) and Stainier (1963) postulated that the chromatophore, as isolated by Schachman and co-workers (1952), is the result of mechanical disruption of the intracytoplasmic membrane. This paper presents evidence that the principal locus of the photosynthetic pigments in R. rubrum is a system of membranes which originate from the peripheral membrane and which form a tubu- lar, branched network in the cytoplasm. MATERIALS AND METHODS Growth of bacteria. R. rubrum (strain S-1) was cultured in liquid medium containing 0.1% NH4Cl, 0.02% MgSO4c7H2O, 0.002% CaC12, 0-05% K2HPO4, 0.1% DL-malate, 0.1% monosodiUm L- glutamate, 0.5% biotin, and 0.13 mg/100 ml of the mixture of trace elements of Aaronson and Baker (1959). The pH was adjusted to 7.2 to 7.4 with NaOH before autoclaving. Cultures were grown phototrophically in test tubes (2.8 X 20.0 cm) or in flat Roux bottles (5 X 10 X 25 cm) in a water bath at 30 C in an enclosure which reduced the ambient light intensity. The cultures were sparged with a mixture of 95% N2 and 5% Co2, at a rate of approximately 100 ml/min. Illumination was provided by 375-w Syl- vania R-32 movie lights adjusted to give a uniform light intensity at the culture vessel. The voltage was stabilized with a constant voltage transformer and set at 62 v a-c with an autotransformer. The light intensity was varied by adjustment of the distance from the lamp to the culture or by inter- posing wire screen rather than by varying the voltage. A photronic cell (Weston Master IV) 1402 on November 6, 2017 by guest http://jb.asm.org/ Downloaded from

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Page 1: BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB ......by Frank Gehry, and the VitraHaus, built by Herzog & de Meuron . Swiss Sophistication: Zurich & Basel Featuring Art

Swiss Sophistication: Zurich & Basel Featuring Art Basel with Christopher Noey, Lecturer

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Travel withThe Met B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B

June 12–20, 2019

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Page 2: BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB ......by Frank Gehry, and the VitraHaus, built by Herzog & de Meuron . Swiss Sophistication: Zurich & Basel Featuring Art

Dear Members and Friends of The Metropolitan Museum of Art,

Switzerland, with its lovely light and brisk mountain air, has been inspiring artists for 200 years. This tiny country has of late become home to some of the most cutting-edge art and design in the world, even as it retains its Old World charm and natural beauty. Please join me next June for a week in this sophisticated country, where a special highlight will be a visit to Art Basel, the unrivaled showcase for contemporary art.

We begin in Zurich, where collections are a must for anyone who loves modern art. Guided by an art expert, tour the Migros Museum of Contemporary Art as well as private galleries tucked inside the former Löwenbräu Brewery. In Lucerne, admire priceless Picassos, Klees, and other modern works housed in a 17th-century palace. Spend four nights in Basel, nestled on the Rhine where France, Germany, and Switzerland meet, to experience Art Basel—where we plan to join other Met curators attending the show—and view the city’s extensive art collection at the Kunstmuseum, founded in 1661. Cross the French border to Colmar, for a wine-tasting lunch and a special viewing of the Isenheim Altarpiece; and marvel at designs by Frank Gehry and Herzog & de Meuron at a campus dedicated to architecture and design on the German side of the Rhine.

Even if you have been to Switzerland before, this amazing itinerary offers much that is new. Travel with The Met programs are limited to a small number of guests, to maintain the intimacy and like-minded camaraderie that are hallmarks of our trips. I urge you to reserve your space as soon as possible, so that you can be with us next June in Zurich and Basel.

Sincerely, Christopher NoeyLecturerThe Metropolitan Museum of Art

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B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B

— Get an insider’s view of Zurich’s Kunsthaus, Migros Museum, and other private galleries with an art expert

— Discover Basel’s Tinguely Museum, dedicated to the fascinating work of the machine sculptor Jean Tinguely, and explore the superb collections of the Kunstmuseum

— View Impressionist and modern masterworks at the Fondation Beyeler, in a building designed by Renzo Piano

— Visit Art Basel, featuring hundreds of galleries displaying the finest modern and contemporary works from around the world, and meet with Met curators attending the show

— Admire the iconic Isenheim Altarpiece in the Unterlinden Museum in Colmar, France, on a special tour

— Cross the German border to visit the Vitra Design Museum, designedby Frank Gehry, and the VitraHaus, built by Herzog & de Meuron

Swiss Sophistication: Zurich & Basel Featuring Art Basel

Traveling with YouChristopher Noey

Christopher Noey is an art historian, curator, and an Emmy award-winning producer of films on art and culture. At The Metropolitan Museum of Art, he created video installations for a diverse array of exhibitions, including Alexander McQueen: Savage Beauty, and Jerusalem 1000-1400: Every People Under Heaven, and he was series director of the innovative online features, “The Artist Project” and “82nd & Fifth.” A specialist in South Asian art, Mr. Noey has taught at Williams College and at the City College of New York.

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Löwenbräukunst, Zurich

Trip Highlights

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G E R M A N Y

Weil Am Rhein

Colmar

Cologne

Basel

S W I T Z E R L A N D

F R A N C E

Bonn

Düsseldorf

Lucerne

Zurich

Rhine

Riv

er

Photos clockwise from top right: Map of the region; Museum Tinguely, Basel; Isenheim Altarpiece, Unterlinden Museum, Colmar; Colmar canal; Fondation Beyeler, Riehen, photo © Swissimages; and Art Basel, Annely Juda Fine Art © Art Basel . Front cover: Sheila Hicks, The Treaty of Chromatic Zones, 2015, Mixed media installation, Approx. 168 x 540 inches (426.7 x 1,371.6 cm), Installation view, Art Basel Unlimited, Basel, Switzerland, 2015, Artwork © Sheila Hicks. Back Cover: Art Basel © Art Basel (top) and Lake Lucerne (bottom).

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Departure Wednesday, June 12 Depart for Zurich on an overnight flight. Zurich, Switzerland Thursday, June 13Arrive in Zurich this morning and transfer to the hotel. In the afternoon, visit the Fraumünster Church to see its stunning stained-glass windows, designed by Marc Chagall. Enjoy a boat cruise on Lake Zurich before our welcome dinner, with remarks by Chris Noey. D Zurich Friday, June 14Begin the morning with a lecture before walking to the Kunsthaus, Zurich’s Museum of Modern Art, for a guided tour. Following lunch, visit the Migros Museum of Contemporary Art and private galleries in the exciting Löwenbräukunst (the former home of Löwenbräu Brewery) in the company of an art expert. B, L

Zurich / LucerneSaturday, June 15On a day trip to lovely Lucerne, explore the beautiful Old Town on a guided walking tour that includes the iconic covered wooden bridge. After free time for lunch, discover the outstanding collection of works by Picasso, Klee, and other greats housed within the Museum Sammlung Rosengart, a grand 17th-century palace. Alternatively, anyone who prefers to have additional time at Art Basel can travel to and from Basel by train today. Dine together in Zurich this evening. B, D

Zurich / BaselSunday, June 16Depart for a morning tour of the charming city of Basel, including the market square and a visit to the Museum Tinguely. The remainder of the day is then dedicated to Art Basel, the outstanding international showcase of modern and contemporary art, with an independent lunch. We plan to meet with one or two Met curators who will also be attending the show. Check into our hotel, where we will have dinner together. B, D

BaselMonday, June 17Following a lecture, admire the Kunstmuseum’s extensive collection during a morning visit. This prominent museum dates back to 1661 and has continued to grow steadily; the most recent building addition—the Neubau —was added in 2016. In the afternoon we hope to view a renowned private collection outside the city. B, L

High Art in the Low Countries Aboard Magnifique III May 17—25, 2019

Japan: Castles, Armor & the Art of the Samurai May 30—June 9, 2019

Undiscovered Greek Islands Aboard Sea Cloud July 21—29, 2019

Ireland’s Historic Homes & Glorious Gardens September 16—24, 2019

For details please go to metmuseum.org/travel

More Travels with The Met

Swiss Sophistication: Zurich & Basel Featuring Art BaselJune 12–20, 2019

Kunsthaus, Zurich © Zurich Tourism / Elisabeth Real

Basel / Colmar, France Tuesday, June 18Drive through vineyards and gentle hills to Colmar, where we will enjoy a special visit to the Unterlinden Museum, housing the Isenheim Altarpiece, a masterpiece of early 16th-century German expressionism. After a wine-tasting lunch, explore the sculpture park and impressive collection of the Fondation Beyeler before returning to the hotel for a free evening. B, L

Fraumünster Church, Zurich

Basel, photo © Swissimages

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To secure your reservation, please call Arrangements Abroad at 844-TRVLMET (844-878-5638); or complete this form and return it with your deposit of $1,000 per person (of which $500 is non-refundable for administrative fees), to be paid by debit card, credit card, wire transfer, or check payable to Arrangements Abroad. Mail to: Arrangements Abroad, 1040 Avenue of the Americas, 23rd Floor, New York, NY 10018-3721.

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Swiss Sophistication: June 12–20, 2019Reservation Form

This itinerary is subject to change at the discretion of The Metropolitan Museum of Art and Arrangements Abroad. For complete details, please carefully read the terms and conditions at www.arrangementsabroad.com/terms.

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Program Rate$7,899 per person, double occupancy$9,299 single rate

Rate Includes — Seven nights accommodations as per itinerary — Meals as indicated in the itinerary (B=Breakfast,

L=Lunch, D=Dinner); bottled water and coffee/tea with all meals, house wine at included dinners

— Enrichment program of lectures and talks

— Escorted sightseeing, transportation, and entrance fees for all included visits

— Airport/hotel/airport group transfers for allparticipants on suggested flights

— Gratuities to local guides and drivers

— Taxes, service, and porterage charges

Not included in rate International airfare; passport/visa fees; meals not specified; alcoholic beverages other than as noted in inclusions; personal items and expenses; trip insurance; baggage in excess of one suitcase; any other items not specifically mentioned as included.

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Hotel Ambassador à l’Opéra, ZurichIn Zurich’s Seefeld district, the Ambassador hotel is part of the former Uto Castle, built in 1898 in neo-Baroque style. It is located directly opposite the opera house and not far from the busy Sächseläutenplatz. The hotel offers a refined, informal ambience. Rooms, fitted with modern bathrooms with a bathtub, have panoramic views of the castle. The hotel’s charming restaurant serves a variety of seafood dishes. Guests can savor tea, cocktails, and coffee in the in-house bar.

Les Trois Rois, BaselThe Grand Hotel Les Trois Rois lies at the very heart of the city of Basel. With over 300 years of history, it has one of the richest traditions of all the hotels in Europe. The Grand Hotel Les Trois Rois has housed royalty, rock legends, and poets. The city’s trade fair, museums, and pedestrian shopping area can all be easily reached on foot. The hotel has 101 elegant rooms and suites. Amenities at the Grand Hotel Les Trois Rois include three restaurants, a bar, and a cigar bar.

Accommodations

Weil Am Rhein, Germany / Basel Wednesday, June 19Cross the German border to discover the Vitra Campus, as well as the Vitra Design Museum, designed by Frank Gehry, and the Vitra-Haus, designed by Herzog & de Meuron. Lunch is at leisure on the grounds. Return to Basel, where the afternoon is free for additional exploring. Gather this evening for our farewell dinner. B, D

Basel / Return Thursday, June 20Depart the hotel this morning for the Zurich airport and flights home. B

Vitra-Haus, Weil am Rhein, photo © Swissimages

Hotel Ambassador à l’Opéra, Zurich (left) and Les Trois Rois, Basel (right)

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The Metropolitan Museum of Art

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Swiss Sophistication:Zurich & Basel Featuring Art Basel June 12–20, 2019

Travel with The Metropolitan Museum of Art

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