automated extraction system method note...

EDGE®

Automated Extraction System

Method Note Compendium

Updated September 25, 2019

Agriculture

Extraction of Pesticides From Avocados 2

Extraction of Polyphenols From Beans 3

Extraction of Polyphenols From Cacao 4

Extraction of Cannabinoids From Cannabis Plant 5

Extraction of Mycotoxins From Cannabis Plant 6

Extraction of Pesticides From Cannabis Plant 7

Extraction of Total Fat From Feeds/Foods 8

Extraction of PCBs From Feeds/Foods (Wet) 9

Extraction of Dioxins From Feeds/Foods (Wet) 10

Extraction of PCBs From Fish Meal (Dry) 11

Extraction of PAHs From Fish Tissue (Dry) 12

Extraction of PAHs From Fish Tissue (Wet) 13

Extraction of Pesticides From Foods (Wet) 14

Extraction of Pesticides From Hops 15

Extraction of Fat From Nuts (Brazil) 16

Extraction of Fat From Nuts (Hazelnuts) 17

Extraction of Fat From Pumpkin Seeds 18

Extraction of Brodifacoum From Rat Poison Powder 19

Extraction of Pesticides From Rice 20

Extraction of PCBs From Soy Meal (Dry) 21

Extraction of Pesticides From Spices (Dry) 22

Extraction of Pesticides From Strawberries 23

Cannabis

Extraction of THC/THCA From Cannabis Flower 25




ContentsEnvironmental & Regulatory

Extraction of SVOCs Environmental Samples 30

Extraction of SVOCs EPA 3545A 31

Extraction of Dioxins From Calcined Kiln Dust 32

Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 33

Extraction of SVOCs From Environmental Samples 34


Extraction of Organics From Lake Sediment 36

Extraction of PCBs From PUF Filters (Large) 37

Extraction of PCBs From PUF Filters (Small) 38

Extraction of PAHs From Quartz Air Filter 39

Extraction of Dioxins From Soil 40

Extraction of Diesel and Oils From Soil 41

Extraction of PAHs From Soil, Loam or Clay 42

Extraction of PCBs From Soil, Loam or Clay 43

Extraction of Dioxins From Soil, Loam or Clay 44

Cleaning of PUF Filters 45

Food Testing

Extraction of Pesticides From Avocados 47

Extraction of Polyphenols From Beans 48

Extraction of Fat From Bone Meal 49

Extraction of Fat From Bread 50

Extraction of Polyphenols From Cacao 51




Extraction of Lipids From Chicken (Baked) 55

Extraction of Lipids From Chicken (Fried) 56

Hydrolysis & Extraction of Total Fat From Chocolate 57

Hydrolysis & Extraction of Total Fat From Cookies 58

Extraction of Fat From DATEM Powder 59

Extraction of Flavonoids from Echinacea 60

Extraction of Fat From Egg Powder 61

Extraction of Total Fat From Feeds/Foods 62

Extraction of PCBs From Feeds/Foods (Wet) 63

Extraction of Dioxins From Feeds/Foods (Wet) 64

Extraction of PCBs From Fish Meal (Dry) 65



Extraction of Pesticides From Foods (Wet) 68

Extraction From Goldenseal Root 69

Extraction of Pesticides From Hops 70

Extraction of Total Fat From Hot Dogs 71

Extraction of PAHs From Infant Formula 72

Extraction of Total Fat From Meats (High-Fat) 73

Extraction of Total Fat From Meats (Potted) 74

Extraction of Fat From Nuts (Brazil) 75

Extraction of Fat From Nuts (Cashews) 76

Extraction of Fat From Nuts (Hazelnuts) 77

Extraction of Total Fat From Nuts (Peanuts) 78

Extraction of Total Fat From Pepperoni 79

Extraction of Lipids From Potatoes (Baked) 80

Extraction of Lipids From Potatoes (Fried) 81

Extraction of Nutrients From Protein Powder 82

Extraction of Total Fat From Protein Powder 83

Extraction of Fat From Pumpkin Seeds 84

Extraction of Pesticides From Rice 85

Extraction of PCBs From Soy Meal (Dry) 86

Extraction of Pesticides From Spices (Dry) 87

Extraction From Spirulina Algae 88

Extraction of Pesticides From Strawberries 89

Extraction of PAHs From Yerba Mate 90

Geoscience & Mining

Extraction of Dioxins From Calcined Kiln Dust 92

Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 93

Extraction of Molybdenum 94

Industrial Hygiene





Materials Science

Extraction of Film Powder 101

Extraction of Pigments From Plastics 102

Extraction of Additives From Polypropylene Powder 103

Extraction of Phthalates From Polyvinyl Chloride 104




Extraction of Antioxidants From Resin 108

Extraction of PAHs From XAD Resin 109


Metals & Alloys

Extraction of Molybdenum 112

Nutraceuticals

Extraction of Flavonoids From Echinacea 114



Extraction From Goldenseal Root 117


Extraction of Nutrients From Protein Powder 119

Extraction of Total Fat From Protein Powder 120

Extraction From Spirulina Algae 121

Extraction of PAHs From Yerba Mate 122

Personal Care Products


Extraction of Mouthwash 125

Extraction of Toothpaste 126

Plastics, Polymers, & Oils

Extraction of Film Powder 128

Extraction of Additives From PET 129

Extraction of Additives From Pigment 130

Extraction of Pigments From Plastics 131

Extraction of Additives From Polyethylene Powder 132

Extraction of Chemical Modifier From Polyethylene Resin 133

Extraction of Additives From Polypropylene Powder 134

Extraction of Phthalates From Polyvinyl Chloride 135

Extraction of Diesel and Oils From Soil 136

Agriculture

1

Extraction of PesticidesFrom AvocadosFrom Avocados

Agriculture, Food TestingEDGE

Note: Temperature and hold times may vary depending on the sample and analytes of interest.

Heating Program

Sample Weight5 g

Q-DiscM2 + C9

EquipmentEDGE40 mL collection vials - clear

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitrile

SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)

Wash Program

Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)

1 4020 1:00010

Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)

1 1% Acetic Acid in Acetonitrile 15 0:1540


General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label2

Extraction of PolyphenolsFrom BeansFrom Beans



Heating Program

Sample Weight0.1 g

Q-DiscC1


NotesNone

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Water

SorbentsN/A

Wash Program


1 8020 10:001010


1 (1:1) Methanol/Water 15 0:1580



label3

Extraction of PolyphenolsFrom CacaoFrom Cacao



Heating Program

Sample Weight0.1 g

Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 8020 10:001010





label4

Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant

Agriculture, Cannabis, Food TestingEDGE


Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9


NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:MethanolIsopropanol

SorbentsN/A

Wash Program


1 9010 0:3055


1 Methanol 30 0:3090

2 Isopropanol 20 0:3090


label5

Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant



Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4


NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9

disc, if M2 used. 2. Weigh the sample into the Q-Cup.

SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed

SorbentsN/A

Wash Program


1 5015 2:0055





label6

Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant



Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9



Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsContents of Agilent QuEChERS SPE 15 mL Tube

Wash Program


1 4020 2:0055





label7

Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods



Heating Program

Sample Weight≤ 5 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsPetroleum Ether

SorbentsWet Samples Only: Sand (use equal amount to sample size)

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Petroleum Ether 15 0:15150



label8

Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsDichloromethane

SorbentsC18 (2.5 g)

Wash Program


1 13520 2:00510


1 Dichloromethane 15 0:15150



label9

Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC4


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.


SorbentsC18 (2 g)

Wash Program


1 15020 2:00510





label10

Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)



Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9


NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

SolventsOptions:Dichloromethane(1:1) Hexane/Acetone

SorbentsC18 (2.5 g)

Wash Program


1 12020 3:001010



2 Extraction Solvent 10 0:00150


label11

Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)

Agriculture, Food Testing, NutraceuticalEDGE


Heating Program

Sample Weight5 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsC18 (1.7 g)

Wash Program


1 12020 3:001010





label12

Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)

Agriculture, Environmental and RegulatEDGE


Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9


NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.



SorbentsC18 (0.8 g)

Wash Program


1 12020 3:001010





label13

Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.


SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)

Wash Program


1 4020 3:00010





label14

Extraction of PesticidesFrom HopsFrom Hops



Heating Program

Sample Weight0.5 g

Q-DiscM2 + C9


NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.


SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used

Wash Program


1 4015 3:00510





label15

Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.


SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label16

Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.


SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label17

Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone



SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label18

Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder

Agriculture, Industrial Hygiene, MaterialEDGE


Heating Program

Sample Weight2 g

Q-DiscC4


NotesNone


SolventsMethanol

SorbentsN/A

Wash Program


1 13015 2:001010

2 13015 2:001010


1 Methanol 15 0:15150

2 Methanol 15 0:00150


label19

Extraction of PesticidesFrom RiceFrom Rice



Heating Program

Sample Weight5 g

Q-DiscM2 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitile


Wash Program


1 4020 3:00010





label20

Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)



Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9


NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsC18 (2.5 g)

Wash Program


1 12020 3:001010





label21

Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9


NotesMatrix matched calibration curve required without the use of sorbents.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 4015 3:00510





label22

Extraction of PesticidesFrom StrawberriesFrom Strawberries



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)

Wash Program


1 4020 3:00010





label23

Cannabis

24

Extraction of THC/THCAFrom Cannabis PlantFrom Cannabis Plant

CannabisEDGE


Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9



Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 9010 0:3055





label25




Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9





SorbentsN/A

Wash Program


1 9010 0:3055





label26




Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4






SorbentsN/A

Wash Program


1 5015 2:0055





label27




Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9






Wash Program


1 4020 2:0055





label28

Environmental & Regulatory

29

Extraction of SVOCsEnvironmental SamplesEnvironmental Samples

Environmental and RegulatoryEDGE


Heating Program

Sample Weight30 g

Q-DiscC4


NotesThis is a general method for the miscellaneous extraction of environmental samples.


SolventsOptions:AcetoneDichloromethaneHexaneToluene

SorbentsN/A

Wash Program


1 12015 2:001010





label30

Extraction of SVOCsEPA 3545AEPA 3545A



Heating Program

Sample Weight30 g

Q-DiscC4


NotesSee the CEM Application note: Extraction of Semi-Volatile Organic Compounds from Soil in Accordance with EPA 3545A if needed.


Solvents(1:1) Acetone/Hexane

SorbentsN/A

Wash Program


1 12015 2:001010


1 (1:1) Acetone/Hexane 30 0:15150

2 (1:1) Acetone/Hexane 30 0:00150


label31

Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust

Environmental and Regulatory, GeoscieEDGE


Heating Program

Sample Weight10 g

Q-DiscC9 + G1 + C9

EquipmentEDGE60 mL collection vials - amber

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:DichloromethaneMethyl tert-butyl ether

SorbentsN/A

Wash Program


1 15020 2:0000

2 15020 2:0000

3 15020 2:0000



2 Methyl tert-butyl ether 20 0:30150


label32

Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix



Heating Program

Sample Weight15 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Dichloromethane

SorbentsN/A

Wash Program


1 10020 3:001010


1 (1:1) Acetone/Dichloromethane 15 0:30150



label33

Extraction of SVOCsFrom Environmental SamplesFrom Environmental Samples



Heating Program

Sample Weight30 g

Q-DiscC4


NotesThis is a general method for the miscellaneous extraction of environmental samples.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

SolventsOptions:Acetone DichloromethaneHexaneToluene

SorbentsN/A

Wash Program


1 12015 2:001010





label34




Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9





SorbentsC18 (0.8 g)

Wash Program


1 12020 3:001010





label35

Extraction of OrganicsFrom Lake SedimentFrom Lake Sediment

Environmental and Regulatory, Organic EDGE


Heating Program

Sample Weight1 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, grind, or cryomill sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

Solvents(1:1) Methanol/Dichloromethane

SorbentsN/A

Wash Program


1 10020 2:0000

2 10020 2:0000


1 (1:1) Methanol/Dichloromethane 15 0:20150


label36

Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)

Environmental and Regulatory, IndustriaEDGE


Heating Program

Sample WeightEntire Filter

Q-DiscC1


NotesNone

Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program


1 11520 3:00155


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label37

Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)



Heating Program


Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.

SolventsHexane

SorbentsN/A

Wash Program


1 10015 0:10100


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label38

Extraction of PAHsFrom Quartz Air FilterFrom Quartz Air Filter



Heating Program

Sample Weight3 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Add the sample on top of the sorbent. No mixing is required.

Solvents(1:1) Hexane/Dichloromethane

SorbentsSand (3 g)

Wash Program


1 12020 2:00010


1 (1:1) Hexane/Dichloromethane 15 0:15100

2 (1:1) Hexane/Dichloromethane 15 0:00100


label39

Extraction of DioxinsFrom SoilFrom Soil



Heating Program

Sample Weight10 g

Q-DiscC1


NotesNone


SolventsOptions:DichloromethaneMethy tert-butyl etherToluene

SorbentsN/A

Wash Program


1 15020 1:0000

2 15020 1:0000

3 15020 1:0000





label40

Extraction of Diesel and OilsFrom SoilFrom Soil

Environmental and Regulatory, Plastics,EDGE


Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.


SorbentsDiatomaceous Earth (1 g)

Wash Program


1 10020 2:0000

2 10010 2:00010





label41

Extraction of PAHsFrom Soil, Loam or ClayFrom Soil, Loam or Clay



Heating Program

Sample Weight30 g

Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 12015 1:001010


1 (1:1) Acetone/Hexane 15 0:30150

2 (1:1) Acetone/Hexane 15 0:00150


label42

Extraction of PCBsFrom Soil, Loam or ClayFrom Soil, Loam or Clay



Heating Program

Sample Weight30 g

Q-DiscC4


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 12015 1:001010


1 (1:1) Acetone/Hexane 15 0:30150

2 (1:1) Acetone/Hexane 15 0:00150

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.d) Bring to volume or evaporate as necessary for analysis.

label43

Extraction of DioxinsFrom Soil, Loam or ClayFrom Soil, Loam or Clay



Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone


SolventsToluene

SorbentsN/A

Wash Program


1 15020 1:0000

2 15020 1:0000

3 15015 1:0005


1 Toluene 30 0:30150

2 Toluene 30 0:30150


label44

Cleaning ofPUF FiltersPUF Filters



Heating Program


Q-DiscC1


NotesNone


SolventsOptions:HexaneAcetone

SorbentsN/A

Wash Program


1 11510 3:00100

2 11510 3:00100

3 11530 3:00100


1 Hexane 15 0:15150

2 Acetone 15 0:00150


label45

Food Testing

46

Extraction of PesticidesFrom AvocadosFrom Avocados



Heating Program

Sample Weight5 g

Q-DiscM2 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.


SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)

Wash Program


1 4020 1:00010





label47

Extraction of PolyphenolsFrom BeansFrom Beans



Heating Program

Sample Weight0.1 g

Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 8020 10:001010




General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label48

Extraction of FatFrom Bone MealFrom Bone Meal

Food TestingEDGE


Heating Program

Sample Weight10 g

Q-DiscC4


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sorbent in the Q-Cup.



Wash Program


1 14020 1:0000

2 14010 1:00010


1 Petroluem Ether 15 0:15150



label49

Extraction of FatFrom BreadFrom Bread

Food TestingEDGE


Heating Program

Sample Weight4 g

Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Dry the sample and mill, or blend, to a fine powder.3. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 14010 1:00010

2 14010 1:00010





label50

Extraction of PolyphenolsFrom CacaoFrom Cacao



Heating Program

Sample Weight0.1 g

Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 8020 10:001010





label51




Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9





SorbentsN/A

Wash Program


1 9010 0:3055





label52




Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9C4






SorbentsN/A

Wash Program


1 5015 2:0055





label53




Heating Program

Sample Weight1.5 g

Q-DiscM2 + C9






Wash Program


1 4020 2:0055





label54

Extraction of LipidsFrom Chicken (Baked)From Chicken (Baked)

Food TestingEDGE


Heating Program

Sample Weight9 g

Q-DiscC1


NotesNone


Solvents(2:1) Chloroform/Methanol

SorbentsN/A

Wash Program


1 3030 4:00010


1 (2:1) Chloroform/Methanol 30 0:2030



label55

Extraction of LipidsFrom Chicken (Fried)From Chicken (Fried)

Food TestingEDGE


Heating Program

Sample Weight9 g

Q-DiscC1


NotesNone


Solvents(2:1) Chloroform/Methanol

SorbentsN/A

Wash Program


1 3030 4:00010




label56

Hydrolysis & Extraction of Total FatFrom ChocolateFrom Chocolate

Food TestingEDGE


Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9


NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.

ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.

SolventsExtraction:Petroleum Ether(25:11) Concentrated Hydrochloric Acid/WaterWash:Water

SorbentsN/A

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Water 15 0:00100



label57

Hydrolysis & Extraction of Total FatFrom CookiesFrom Cookies

Food TestingEDGE


Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9


NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.

ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.

SolventsExtraction:Petroleum Ether(25:11) Concentrated Hyrdochloric Acid/WaterWash:Water

SorbentsN/A

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Water 15 0:00150



label58

Extraction of FatFrom DATEM PowderFrom DATEM Powder

Food TestingEDGE


Heating Program

Sample Weight1 g

Q-DiscC4


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.3. Weigh the sand directly on top of the sample to compact down.


SorbentsSand (1 g)

Wash Program


1 10010 1:0000

2 10010 1:0000

3 10010 1:0000

4 1005 1:0005





59

Extraction of FlavonoidsFrFromom Echinacea

Food TestingEDGE

label


Heating Program

Sample Weight2 g

Q-DiscM2 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of theC9 Q-Disc.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 6020 1:00020


1 (70:30) Methanol/Water 15 0:1060

2 (70:30) Methanol/Water 10 0:0060

General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.

label 60

Extraction of FatFrom Egg PowderFrom Egg Powder

Food TestingEDGE


Heating Program

Sample Weight5 g

Q-DiscC4


NotesNone


SolventsToluene

SorbentsN/A

Wash Program


1 14020 1:0000

2 14015 1:0005


1 Toluene 15 0:15150

2 Toluene 15 0:00150


label61

Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods



Heating Program

Sample Weight≤ 5 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.


SorbentsWet Samples Only: Sand (use equal amount to sample size)

Wash Program


1 14010 1:00010

2 14010 1:00010





label62

Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone



SorbentsC18 (2.5 g)

Wash Program


1 13520 2:00510





label63

Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC4


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsOptions:DichloromethaneMethyl tert-butyl etherToluene

SorbentsC18 (2 g)

Wash Program


1 15020 1:0000

2 15020 1:0000

3 15020 1:0000





label64

Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)



Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9


NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsC18 (2.5 g)

Wash Program


1 12020 3:001010





label65




Heating Program

Sample Weight5 g

Q-DiscC1


NotesNone



SorbentsC18 (1.7 g)

Wash Program


1 12020 3:001010





label66




Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9





SorbentsC18 (0.8 g)

Wash Program


1 12020 3:001010





label67

Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.



Wash Program


1 4020 3:00010





label68

ExtractionFrom Goldenseal RootFrom Goldenseal Root

Food Testing, NutraceuticalsEDGE


Heating Program

Sample Weight5 g

Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.

SolventsAcetonitrile

SorbentsN/A

Wash Program


1 10015 1:00105


1 Acetonitrile 15 0:15150



label69

Extraction of PesticidesFrom HopsFrom Hops



Heating Program

Sample Weight0.5 g

Q-DiscM2 + C9


NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.


SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used

Wash Program


1 4015 3:00510





label70

Extraction of Total FatFrom Hot DogsFrom Hot Dogs

Food TestingEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.

SolventsExtraction:Petroleum EtherWash:Water

SorbentsSand (2 g)

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Water 10 0:00150



label71

Extraction of PAHsFrom Infant FormulaFrom Infant Formula

Food Testing, Nutraceuticals, Personal EDGE


Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.

SolventsCyclohexane

SorbentsN/A

Wash Program


1 12020 1:00010


1 Cyclohexane 15 0:30150



label72

Extraction of Total FatFrom Meats (High-Fat)From Meats (High-Fat)

Food TestingEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.


SorbentsSand (2 g)

Wash Program


1 14010 1:00010

2 14010 1:00010





label73

Extraction of Total FatFrom Meats (Potted)From Meats (Potted)

Food TestingEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone


SolventsExtraction:Petroleum EtherWash:Water

SorbentsSand (2 g)

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Water 10 0:00150



label74

Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone



SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label75

Extraction of FatFrom Nuts (Cashews)From Nuts (Cashews)

Food TestingEDGE


Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.


SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label76

Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone



SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label77

Extraction of Total FatFrom Nuts (Peanuts)From Nuts (Peanuts)

Food TestingEDGE


Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone



SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label78

Extraction of Total FatFrom PepperoniFrom Pepperoni

Food TestingEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone



SorbentsSand (2 g)

Wash Program


1 14010 1:00010

2 14010 1:00010


1 Water 10 0:00150



label79

Extraction of LipidsFrom Potatoes (Baked)From Potatoes (Baked)

Food TestingEDGE


Heating Program

Sample Weight9 g

Q-DiscC1


NotesNone


Solvents(3:2) Hexane/Isopropanol

SorbentsN/A

Wash Program


1 3030 4:00010


1 (3:2) Hexane/Isopropanol 30 0:2030



label80

Extraction of LipidsFrom Potatoes (Fried)From Potatoes (Fried)

Food TestingEDGE


Heating Program

Sample Weight9 g

Q-DiscC1


NotesNone


Solvents(3:2) Hexane/Isopropanol

SorbentsN/A

Wash Program


1 3030 4:00010




label81

Extraction of NutrientsFrom Protein PowderFrom Protein Powder



Heating Program

Sample Weight1 g

Q-DiscC4


NotesNone



SorbentsSand (3 g)

Wash Program


1 15015 0:101010


1 (80:20) Methanol/Water 15 0:15150

2 (80:20) Methanol/Water 15 0:00150


label82

Extraction of Total FatFrom Protein PowderFrom Protein Powder



Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone



SorbentsSand (6 g)

Wash Program


1 12015 2:00105





label83

Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds



Heating Program

Sample Weight12 g

Q-DiscC1


NotesNone



SorbentsSand (12 g)

Wash Program


1 14020 1:0000

2 14015 1:0005





label84

Extraction of PesticidesFrom RiceFrom Rice



Heating Program

Sample Weight5 g

Q-DiscM2 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.

Solvents1% Acetic Acid in Acetonitile


Wash Program


1 4020 3:00010





label85

Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)



Heating Program

Sample Weight5 g

Q-DiscC1M1 + C9


NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsC18 (2.5 g)

Wash Program


1 12020 3:001010





label86

Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9


NotesMatrix matched calibration curve required without the use of sorbents.

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 4015 3:00510





label87

ExtractionFrom Spirulina AlgaeFrom Spirulina Algae



Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.

2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 10015 1:00105





label88

Extraction of PesticidesFrom StrawberriesFrom Strawberries



Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.


SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)

Wash Program


1 4020 3:00010





label89

Extraction of PAHsFrom Yerba MateFrom Yerba Mate



Heating Program

Sample Weight0.5 g

Q-DiscC1


NotesNone


SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone

SorbentsN/A

Wash Program


1 120-15015 1:00105





label90

Geoscience & Mining

91

Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust



Heating Program

Sample Weight10 g

Q-DiscC9 + G1 + C9

EquipmentEDGE60 mL collection vials - amber

NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.

SolventsOptions:DichloromethaneMethyl tert-butyl ether

SorbentsN/A

Wash Program


1 15020 2:0000

2 15020 2:0000

3 15020 2:0000



2 Methyl tert-butyl ether 20 0:30150


label92

Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix



Heating Program

Sample Weight15 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.

Solvents(1:1) Acetone/Dichloromethane

SorbentsN/A

Wash Program


1 10020 3:001010





label93

Extraction ofMolybdenumMolybdenum

Geoscience and Mining, Metals and AllEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone


SolventsAcetone

SorbentsN/A

Wash Program


1 14015 1:00105


1 Acetone 15 0:15140

2 Acetone 15 0:00140


label94

Industrial Hygiene

95




Heating Program


Q-DiscC1


NotesNone


SolventsHexane

SorbentsN/A

Wash Program


1 11520 3:00155


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label96




Heating Program


Q-DiscC1


NotesNone


SolventsHexane

SorbentsN/A

Wash Program


1 10015 0:10100


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label97




Heating Program

Sample Weight2 g

Q-DiscC4


NotesNone


SolventsMethanol

SorbentsN/A

Wash Program


1 13015 2:001010

2 13015 2:001010


1 Methanol 15 0:15150

2 Methanol 15 0:00150


label98




Heating Program


Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 11510 3:00100

2 11510 3:00100

3 11530 3:00100


1 Hexane 15 0:15150

2 Acetone 15 0:00150


label99

Materials Science

100

Extraction ofFilm PowderFilm Powder

Materials Science, Plastics, Polymers, aEDGE


Heating Program

Sample Weight1 g

Q-DiscM1 + C9


Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.

Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.

SolventsOptions:CyclohexaneDichloromethane

SorbentsN/A

Wash Program


1 *40-7015 10:0005





label101

Extraction of PigmentsFrom PlasticsFrom Plastics



Heating Program

Sample Weight1 g

Q-DiscM1 + C9





SorbentsN/A

Wash Program


1 *40-8015 10:0005





label102

Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9



Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program


1 12030 15:00010





label103

Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9



Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup

Solvents(1:1) Isopropanol/Cyclohexane

SorbentsN/A

Wash Program


1 *8020 10:00010


1 (1:1) Isopropanol/Cyclohexane 10 0:1580



label104




Heating Program


Q-DiscC1


NotesNone


SolventsHexane

SorbentsN/A

Wash Program


1 11520 3:00155


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label105




Heating Program


Q-DiscC1


NotesNone


SolventsHexane

SorbentsN/A

Wash Program


1 10015 0:10100


1 Hexane 10 0:15150

2 Hexane 10 0:00150


label106




Heating Program

Sample Weight2 g

Q-DiscC4


NotesNone


SolventsMethanol

SorbentsN/A

Wash Program


1 13015 2:001010

2 13015 2:001010


1 Methanol 15 0:15150

2 Methanol 15 0:00150


label107

Extraction of AntioxidantsFrom ResinFrom Resin

Materials ScienceEDGE


Heating Program

Sample Weight1 g

Q-DiscC1


NotesNone


SolventsMethanol

SorbentsN/A

Wash Program


1 10015 2:00510


1 Methanol 15 0:20150


label108

Extraction of PAHsFrom XAD ResinFrom XAD Resin

Materials ScienceEDGE


Heating Program

Sample Weight15 g

Q-DiscC1


NotesBe sure that sample or sand is present in the Q-Cup when running this method. Sample size does matter, as you decrease the sample size you will build up more pressure within the cell.



SorbentsN/A

Wash Program


1 15020 2:001010





label109




Heating Program


Q-DiscC1


NotesNone



SorbentsN/A

Wash Program


1 11510 3:00100

2 11510 3:00100

3 11530 3:00100


1 Hexane 15 0:15150

2 Acetone 15 0:00150


label110

Metals & Alloys

111

Extraction ofMolybdenumMolybdenum

Geoscience and Mining, Metals and AllEDGE


Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone


SolventsAcetone

SorbentsN/A

Wash Program


1 14015 1:00105


1 Acetone 15 0:15140

2 Acetone 15 0:00140


label112

Nutraceuticals

113

Extraction of FlavonoidsFrom EchinaceaFrom Echinacea



Heating Program

Sample Weight2 g

Q-DiscM2 + C9


NotesNone

Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 6020 1:00020


1 (70:30) Methanol/Water 15 0:1060

2 (70:30) Methanol/Water 10 0:0060


label114




Heating Program

Sample Weight5 g

Q-DiscC1


NotesNone



SorbentsC18 (1.7 g)

Wash Program


1 12020 3:001010





label115




Heating Program

Sample Weight2 g

Q-DiscC9 + G1 + C9





SorbentsC18 (0.8 g)

Wash Program


1 12020 3:001010





label116

ExtractionFrom Goldenseal RootFrom Goldenseal Root



Heating Program

Sample Weight5 g

Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 10015 1:00105





label117




Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9


NotesNone


SolventsCyclohexane

SorbentsN/A

Wash Program


1 12020 1:00010





label118

Extraction of NutrientsFrom Protein PowderFrom Protein Powder



Heating Program

Sample Weight1 g

Q-DiscC4


NotesNone



SorbentsSand (3 g)

Wash Program


1 15015 0:101010


1 (80:20) Methanol/Water 15 0:15150

2 (80:20) Methanol/Water 15 0:00150


label119

Extraction of Total FatFrom Protein PowderFrom Protein Powder



Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone



SorbentsSand (6 g)

Wash Program


1 12015 2:00105





label120

ExtractionFrom Spirulina AlgaeFrom Spirulina Algae



Heating Program

Sample Weight2 g

Q-DiscC1


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®.

2. Weigh the sample into the Q-Cup.


SorbentsN/A

Wash Program


1 10015 1:00105





label121

Extraction of PAHsFrom Yerba MateFrom Yerba Mate



Heating Program

Sample Weight0.5 g

Q-DiscC1


NotesNone


SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone

SorbentsN/A

Wash Program


1 120-15015 1:00105





label122

Personal Care Products

123




Heating Program

Sample Weight4 g

Q-DiscC9 + G1 + C9


NotesNone


SolventsCyclohexane

SorbentsN/A

Wash Program


1 12020 1:00010





label124

Extraction ofMouthwashMouthwash

Personal Care (Health and Beauty)EDGE


Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.

2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. 3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.

SolventsHexane

SorbentsOptions:Sodium Polyacrylate (5 g)Diatomaceous Earth (5 g)

Wash Program


1 4020 3:00010


1 Hexane 15 0:15100

2 Hexane 15 0:00100


label125

Extraction ofToothpasteToothpaste

Personal Care (Health and Beauty)EDGE


Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


Notes*for sand- mix into sample*for Diatamaceous Earth- layer under sample, do not mix.

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent/sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup*.

SolventsHexane

SorbentsOptions:Sand (15 g)Diatamaceous Earth (5 g)

Wash Program


1 4020 3:00010


1 Hexane 15 0:1540

2 Hexane 15 0:0040


label126

Plastics, Polymers, & Oils

127

Extraction ofFilm PowderFilm Powder



Heating Program

Sample Weight1 g

Q-DiscM1 + C9





SorbentsN/A

Wash Program


1 *40-7015 10:0005





label128

Extraction of AdditivesFrom PETFrom PET

Plastics, Polymers, and OilsEDGE


Heating Program

Sample Weight1 g

Q-DiscC1





SorbentsN/A

Wash Program


1 115*25 5:00510


1 (40:60) Acetone/Hexane 15 0:15150

2 (40:60) Acetone/Hexane 15 0:00150


label129

Extraction of AdditivesFrom PigmentFrom Pigment



Heating Program

Sample Weight1 g

Q-DiscC1


NotesNone


SolventsDimethylformamide

SorbentsN/A

Wash Program


1 10020 1:001010


1 Dimethylformamide 10 0:15125

2 Dimethylformamide 10 0:00125


label130

Extraction of PigmentsFrom PlasticsFrom Plastics



Heating Program

Sample Weight1 g

Q-DiscM1 + C9





SorbentsN/A

Wash Program


1 *40-8015 10:0005





label131

Extraction of AdditivesFrom Polyethylene PowderFrom Polyethylene Powder



Heating Program

Sample Weight1 g

Q-DiscM1 + C9



Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the C9 Q-Disc on top of the M1 Q-Disc. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program


1 *9030 15:00010





label132

Extraction of Chemical ModifierFrom Polyethylene ResinFrom Polyethylene Resin



Heating Program

Sample Weight0.1 g

Q-DiscC1





SorbentsNone

Wash Program


1 *6020 15:001010


1 (40:60) Acetone/Hexane 10 0:1560

2 (40:60) Acetone/Hexane 10 0:0060


label133

Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9



Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.

SolventsIsopropanol

SorbentsN/A

Wash Program


1 12030 15:00010





label134

Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride



Heating Program

Sample Weight1 g

Q-DiscC9 + G1 + C9



Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup

Solvents(1:1) Isopropanol/Cyclohexane

SorbentsN/A

Wash Program


1 *8020 10:00010





label135

Extraction of Diesel and OilsFrom SoilFrom Soil

Environmental and Regulatory, Plastics,EDGE


Heating Program

Sample Weight5 g

Q-DiscC9 + G1 + C9


NotesNone

Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.



Wash Program


1 10020 2:0000

2 10010 2:00010





label136

automated extraction system method note...

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