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A facile method for the preparation of chitosan-based scaffolds

with anisotropic and inter-connective pore structures for tissue

engineering applications

Jiabing Ran, Lingjun Xie, Guanglin Sun, Jingxiao Hu, Si Chen, Pei Jiang, Xinyu

Shen*, and Hua Tong*

Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry

and Molecular Sciences, Wuhan University, Wuhan, 430072, China.

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Figure. S1 SM images of the cross-section of (A) upper part, (B) middle part, and (C)

lower part of sample 2#; the statistical chart right next to each image shows the

corresponding pore diameter distribution.

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Figure. S2 SM images of the cross-section of bottom of CS/HAp, CS/PVA, CS/GEL,

CS/SF, and CS-SF/HAp hydrogels.

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Figure. S3 (A) stress-strain curves of CS, CS/HAp, CS/SF, and CS-SF/HAp scaffolds.

The insert shows the stress-strain curves of the four scaffolds with strain ranging

between 0 and 0.1; (B) histograms of the elastic modulus and compressive modulus at

strain of 0.7 of CS, CS/HAp, CS/SF, and CS-SF/HAp scaffolds.