SALMON EGGS 2013/2014DELIVERED OVER 10 MONTHS, FROM OCTOBER TO AUGUST

seeds of vitalityand profitability

AquaGen

AquaGen carries out several selections of brood-stock at different levels before the final selection is allowed to produce eggs for the farmers. The selection process occurs both at the family level and at the individual level. The salmon eggs that AquaGen will deliver are the results of selective breeding for a range of impor-tant traits through 10 generations. How long the different traits have been selected for, as well as the intensity of the selection is very important for the total progress which today’s products repre-sent. At the present time a total of 22 traits are recorded from 800 families. A comprehensive recording program ensures progress in these de-sirable traits while at the same time minimalizing the risk of unwanted or unexpected side effects.

AquaGen has two main groups of broodstock which are divided according to the following cat-egorization of traits:

1. Effective (E). Special emphasis on production and qualitative traits.

2. Robust (R). Special emphasis on resistance and robustness traits.

Crossing fish from the two groups will produce descendants with different profiles for the desired traits.

Genetic Background for Salmon Eggs 2013/2014

1972-75: 2005:First feeding year for juveniles of brood fish The four year classes collected from 41 Norwegian salmon rivers. were merged into one.

72 76 80 84 88 92 96 00 04Class of Year 73 77 81 85 89 93 97 01(first feeding) 74 78 82 86 90 94 98 02

75 79 83 87 91 95 99 03GROWTH SEA WATER

SEXUAL MATURATION

ISAFILET FAT

SKELETAL DEFORMITIESProduction/Efficiency TraitsHealth/Robustness TraitsThe top-ranking families first fed in 2010 are the base for brood fish stripping in 2013/2014

FILET YIELD

Traits

0 1 2 3Generation

864

BODY SHAPE

FILET COLOUR

05 06 07 08 10

GROWTH FRESH WATERIPN

09

SLAUGHTER YIELD

FURUNCULOSIS

Implementation of traits based on traditional selective breeding methods in AquaGen Atlantic Salmon (Salmo salar )

5 97

The figure below shows the main traits which are measured, selected and controlled in the selective breeding program (breeding nucleus) set up in relation to the cohort/generation to be phased in.

Salmon eggs which will be delivered to our cus-tomers hatcheries in the 2013/2014 season will be produced from the top-ranking families that were first fed in 2010 (marked with grey in the chart). Elite material from this generation is transferred to our egg producers. After a four year production cycle the best individuals are se-lected as broodstock. In this way the best genet-ics will be reproduced on a large scale to benefit all fish farmers.

Since 2009 AquaGen has implemented new mod-ern technology called Marker Assisted Selection. Based on this new technology platform we are able to directly select parents to be used in egg-production that possess specific genetic markers (QTLs) that are highly associated with resistance to specific viral diseases. This double selection, both on family- and individual level, ensures a balanced weight to robust and effective traits as well as high IPN-, PD resistance and/or strong fi-let colour in the offspring.

Biosecurity and Quality ControlFish health surveillance in AquaGen is carried out through the entire life-cycle of the fish. In the final nine months before stripping the monitoring is intensified. This includes comprehensive autopsy and screening of all broodstock populations in the AquaGen system.

Production is monitored by both internal and external fish-health personnel, and by government and private entities. AquaGen is certified according to GLOBAL GAP (food safety, environmental protection, fish welfare, and health, safety and welfare for employees), FREEDOM FOOD (animal welfare), ISO 9001:2008 (quality lead-ership) and Code-EFABAR

(good practice within animal farm breeding and reproduction).

AquaGen’s production plants at Hemne and Tingvoll are located in the EU-approved ISA-free compartments at the highest security level. ISA has never been detected in these compartments.

AquaGen’s broodstock populations are routinely screened for IPN virus, ISA virus, PD virus and the BKD bacterium during the entire production cycle of four years.

Individual testing of parents at stripping can be carried out in addition if ordered in advance.

Relevant health information about brood fish and eggs will be documented in a separate HEALTH CV and included in all egg deliveries.

Delivery Time In the egg season of 2013/2014 AquaGen will deliver eggs over 10 months, from late October 2013 to August 2014:

Category Delivery Time

• Early eggs week 43-50 (Oct. 21 - Dec. 15)• Normal eggs week 51-13 (Dec. 16 - Mar. 30)• Late eggs week 14-35 (Mar. 31 - Aug. 31)

In the figure below the differing production time-lines are presented for salmon through the fresh-water phase and the salt-water phase, based on early, normal and late eggs. The figure shows how AquaGen by means of managed production of broodstock can contribute to the optimal exploita-tion of our customer’s production facility capacity.

If there should be an extra need for eggs as a re-sult of operating accidents, diseases or lack of ca-pacity in own facility, AquaGen often has the abil-ity to help out with deliveries in such situations.

Production plan for salmon in the FRESH-WATER PHASECategory

Oct Nov Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun0-year Early eggs 0-/1-year During the winter the smolt can also be kept in salt water 0-year in tanks and transfered to sea in the spring.

Production plan for salmon in the SEA-WATER PHASECategory

Jul Aug Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar0-year 0-/1-year 0-year

SmoltHarvest

4th year in sea1st year in sea

HarvestHarvestSmolt

Smolt

Smolt

Sep3rd year in sea

Smolt SmoltSmolt

2nd year in sea

Late eggsNormal eggs

Smolt

1st year 2nd yearDes

3rd year

Early ordering, preferably before July 1, 2013 contributes to better production planning and higher de-livery assurance in regard to choice of product type, amount and time of delivery. Depending on the results from genotyping of this season’s broodstock, some types of products have limited availability.

Product Description

Eggs

AquaGen® Atlantic QTL-innOva®

IPN Eggs from broodstock which produces a balance between Robust and Effec-tive traits as well as extra high IPN resistance (RPS=82 %) in the offspring. The males are chosen using the genetic marker for IPN.

IPN/RED Eggs from broodstock which produces a balance between Robust and Effec-tive traits as well as extra high IPN resistance (RPS=82 %) and strong filet colour in the offspring. The males are chosen using the genetic markers for IPN and filet colour.

IPN/PD Eggs from broodstock which produces a balance between Robust and Effec-tive traits as well as extra high IPN resistance (RPS=82 %) and PD resistance in the offspring. The males are chosen using the genetic markers for IPN and PD.

IPN/PD/RED Eggs from broodstock which produces a balance between Robust and Effec-tive traits as well as extra high IPN resistance (RPS=82 %), PD resistance and strong filet colour in the offspring. The males are chosen using the ge-netic markers for IPN, PD and filet colour.

Trea

tmen

t

Treatment of broodstock/eggs

Sterilization Sterilization of eggs which after pressure treatment on recently fertilized eggs makes the fish sterile. This procedure can be done on all types of eggs listed above.

Virus testing Individual testing of female- and male broodstock for IPNV, PDV and/or ISAV at the time of stripping. Broodstock free from virus are used in the egg pro-duction only.

Robust (R) = Health-related traits such as resistance to disease, reduced deformities, reduced early sexual maturity.

Effective (E) = Production-related traits such as growth, filet color, fat content, harvest amounts, body form.

QTL = Gene marker (Quantitative Trait Loci). Area on the salmon genome (marker) which is closely connected to a gene which controls a specific characteristic in the individual.

RPS = Relative percent survival after IPN challenge.

Product Overview for Salmon Eggs 2013/2014

AquaGen AS • PO Box 1240 • Sluppen • N-7462 Trondheim

Phone: +47 72 45 05 00 • Fax: +47 73 54 62 91

firmapost@aquagen.no • www.aquagen.no/enseeds of vitalityand profitability

AquaGen