aquaculture products in vietnam

8/18/2019 AQUACULTURE PRODUCTS IN VIETNAM

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TECHINAL IMPROVEMENT OF MARINE

FIN FISH HATCHERY PRODUCTION

USING INVE AQUACULTURE PRODUCTSIN VIETNAM

Nguyen Huu Dung

Regional Technical and Sale Support Manager

E-mail: [email protected]

Mobil. +84 94 724 8778


2/31

A STUDY ON STATUS OF MARINE FIN

FISH HATCHERY PRODUCTION IN

VIETNAMPurpose: Understand status of techniques, ways of using

INVE products in fin fish hatcheries in order to suggest

technical recommendations on appropriate application of

INVE product to improve the hatchery productivity.

Time: 15 September – 31 December, 2015

Targets:

– 14 hatcheries in Khánh Hòa province

– 11 hatcheries in Bà Rịa – Vũng Tàu province,

– Yet the new hatcheries established in 2015 or those of seasonal

operation, hatcheries invested by Chinese and Taiwanese, or those

applying mesocosmic nursing systems.


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POPULARLY APPLIED PROTOCOL

Algae

Rotifer

Artemia

Formulated feeds

DPH

0 402 251410 18 60 80


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INVE PRODUCTS HAVE BEEN USED IN

VIETNAMESE FISH HATCHERIES

Algae

Rotifer

Artemia

Formulated feeds

DPH

0 402 251410 18 60 80

Rotifer feed: S-parkle

Enrichment: DHA Protein Selco

Cyst: Hi5, GSL, SepArt

Enrich diet: A1 DHA Selco

NRD ½; 2/3; 3/5; 5/8

NRD G8; G12; P16


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PRODUCTION OF SURVEYED

HATCHERIS

Fish speciesSize in

TL (cm)

Production

(1000 pcs)

Survival rate

(%)

Comp. to

2014 (%)

Asian sea bass 2-3 61.800 25 – 40 - 20

Pompanos 3-4 1.610 25 – 30 + 10

Cobia 10-12 520 1,5 – 2,0 + 70

Groupers 6-8 140 0,1 - 2,0 + 60

Others - 300 - + 20


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IMPORTANT ISSUE OF VIETNAMESE

MARINE FIN FISH HATCHERY PRODUCTION

Very high deformityrate

– Sea bass: 10 – 80%,

– Cobia: 10 – 15% – Groupers: 8 – 10%

– Pompanos: 10 – 35%


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GWS – GREEN WATER SUBTITUTION

Solution for Green Water in Hatchery

Rotifer feeding stage

Benefits – Reduce strees due to light

– Maintain rotifer quality

– Improve feeding efficiency


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GWS: ADVANTAGES AND SUGGESTED

APPLICATION IN VIETNAM

Microalgae culture

– Unstable at production sites

– Risk of contamination

Sanolife GWS application – Once/day in early morning

– 4 g/m3/day

– Well mixed with clean water,

keep overnight at 4-6oC

– Re-mix again before adding

to fish tank


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ROTIFER CULTURE – VIETNAM

EXPERIENCES

Correctly quantify before/in order to feeding your

rotifer NOT ESTIMATEDLY QUANTIFICATION

Use appropriate ciliate trap & debris collectors

Improve tools for rotifer collection


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HARVEST ROTIFER USING FLOWTHROUGH COLLECTOR


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APPLICATION OF S-PARKLE TO

FEED ROTIFER


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APPLICATION OF S-PARKLE


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APPLICATION OF S-PARKLE IN

VIETNAM

Rotifer density (rot./mL) Feeding rate (g/mill. Rot./day)

200 – 300 0.50

300 – 500 0.45

500 – 700 0.40

700 – 900 0.35

> 900 0.30

Notes: Lower feeding rate as compared to European

condition due to dissolved oxygen limitation.


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ROTIFER ENRICHMENT PROTOCOL

Rotifer density: 1000 rot. / mlTime: max 6 hours

Enrichment diet: DHA Protein

Selco

Mix with potable water, keep inrefrigerator for 6 hrs, re-mix again

before adding to enriching tank.

Final dose: 200 g/m3:

– At starting time: 100 g/m3 – Three hours later: add 100 g/m3

REPLACE AIRSTONE WITH

Tube with 1.5mm diameter

holes


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GENERAL PROBLEMS OF ARTEMIA INCUBATION

AND ENRICHMENT IN VIETNAM

Aeration with airstone or open tube Natural light or néon lamp, light intensity

1200 Lux

Not fully separate cyst shells from nauplii

Evaluate hatching efficiency by wet weight Nauplii are not cleaned at enriching time

Low density (150 NPL/ml) and low

concentration (150 g/m3)

Enriching time too short (6 hrs)Egg incubation and NPL enriching many

batches per day – coincide with feeding

frequency

HIGH COST – ENRICHED ARTEMIA QUALITY LOWER THAN FISH REQUIREMENT


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RECOMMENDED TYPE OF ARTEMIA

SepArt helps fullseparation of cyst shell

from nauplii at harvesting


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INVE AQUACULTURE - NGOC TRAI - SEMINAR - 2016


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ARTEMIA INCUBATION


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ARTEMIA INCUBATION

RECOMMENDATIONS

USE DRILLED TUBES

USE LED LAMP FOR HIGHER LIGHT INTENSITY ≥ 2500 LUX

INCUBATION TIME NOT MORE THAN 24 hours

CONTROL OF MICROBIAL DEVELOPMENT DURING INCUBATION


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CONTROL OF pH and MICROBIAL

DEVELOPMENT DURING INCUBATION

ACE

A1 DHA SELCO


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APPLICATION OF ACE IN

INCUBATION TANK

Without ACE With ACE


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ARTEMIA NAUPLII HARVESTING

• Create light source at the tank bottom

• Use Nauplii collector for more viable and clean naupliii


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SUGGESTED ARTEMIA NAUPLII

ENRICHMENT FOR VIETNAMESE

HATCHERY NPL density: 500 NPL / ml

Enrichment period: 20-22 hrs

Enrichment diet: A1 DHA Selco

Mix with potable water, keep inrefrigerator for 6 hrs, re-mix again

before adding to enriching tank.

Final concentration: 400 g/m3, and:

– Starting time: 200 g/m3

– 10 hrs later: add 200 g/m3

REPLACE AIR STONES WITH

1.5mm holes TUBE


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0%

20%

40%

60%

80%

100%

120%

0 6 12 18 24

E n r i c

h m e n t E f f i c i e n c y

HUFA n-3 ≥20:3

During the first 14 h of enrichment rate reach 6% per hour.

The subsequent hours, from 14h –

24 h, the enrichmentreaches a rate of 2% per hour.


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0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

- - - - - 6 12 18 24

0 6 12 18 24 - - - -

EASY DHA enrichment Cold Storage

H U F A

( m g / g d w )

Enrichment kinetics GSL2010 + Cold storage

ARA 20:4(n-6) EPA 20:5(n-3) DHA 22:6(n-3)

After 24 hours, with 600 ppm Easy DHA Selco + ACE reach approximately 55 mg HUFA (n-3 ≥20:3)/g

dry weight. A typical DHA/EPA ratio of 1,4 can easily

be obtained when using Easy DHA Selco®.


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COLD STORAGE OF ENRICHED ARTEMIA

NAUPLII TO SAVE YOUR LABOUR

IARC’s COOL STORAGE

Made in DenmarkLOCAL MADE COOL STORAGEMade in Nha Trang – VIETNAM


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Tank volume: 10 m3 Water Depth: 100 cm Stock ing density: 100-120 ferti lized eggs per l iter Hatch ing rate : ca. 95% Temp. : 28 - 30oC

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 3

GWS (g/m3) 7:00 AM 1.0 2.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 3.0 3.0 1.0 2.0 2.0 1.0 - - - - - - - - - - - -

7:00 AM - - 1.0 2.0 4.0 6.0 8.0 10.0 10.0 8.0 6.0 4.0 2.0 - - - - - - - - - - - - - - - - - -

15:00 PM - - 2.0 3.0 5.0 7.0 9.0 10.0 9.0 7.0 5.0 3.0 1.0 - - - - - - - - - - - - - - - - - -

7:00 AM - - - - - - - - 1.0 2.0 3.0 4.0 - - - - - - - - - - - - - - - - - - -

15:00 PM - - - - - - - - 2.0 3.0 4.0 3.0 - - - - - - - - - - - - - - - - - - -

7:00 AM - - - - - - - - - - - 2.0 5.0 6.0 7.0 9.0 10.0 10.0 10.0 11.0 12.0 12.0 12.0 12.0 11.0 10.0 9.0 8.0 7.0 6.0 -

15:00 PM - - - - - - - - - - - 3.0 6.0 7.0 8.0 10.0 10.0 11.0 11.0 12.0 13.0 13.0 13.0 12.0 11.0 11.0 10.0 9.0 8.0 5.0 3.

O -R ang e 1/2 - - - - - 0.5 0.6 0.7 0.8 0.9 1.0 1.0 1.0 1.0 0.5 0.3 0.2 - - - - - - - - - - - - - -

O -R ang e 2/3 - - - - - - - - - - - - 0.5 1.0 1.5 1.8 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 5.

O -R ang e 3/5 - - - - - - - - - - - - - - - - - - - - - 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 20.

Water exchange rate (Tank volume percentage) 16:00 PM - 10.0 - - 10.0 - - 10.0 - - 15.0 - - 15.0 - - 20.0 - 100 - 20.0 - 20.0 - 20.0 20.0 20.0 20.0 20.0 20.0 100.

Rotifer enrichment notes (ToC= 28-32oC)

Density: max. 1000 rot./ml

Time: max. 6hrs

Enrichment diet: DHA Protein Selco

Diet accumulated dose = 150 ppm, of which:

150 ppm at starting time (T0), and

150 ppm at 3hr post-start (T3)

eration: TUBE WITH 1.5mm holes AERATION

Enrichment diet: A1 DHA Selco

Diet accumulated dose = 400 ppm, of which:

200 ppm at starting time (T0), and

200 ppm at T12

Aeration: TUBE WITH 1.5mm holes AERATION

Enriched GSL Artemia feeding (Nau./ml)

Micro-diet weaning (g/m3/day)

Artemia enrichment notes (ToC= 28-32oC)

Harvest Nauplii at 24 hrs post-incubation

Density: max. 400 Nauplii/ml

Time: max. 22 hrs

FEEDING TIME TABLE FOR HATCHERY PRODUCTION OF BARRAM UNDI

ApplicationsWhen /

What

Days Post-hatching

Enriched rotifer feeding (rot./ml)

Non-enriched AF Artemia umbrela (Instar 1/ml)


28/31

APPLICATION OF IMPROVED

TECHNIQUES IN VIETNAM

Center for Aquatic Animal Health and Breeding Studies – 3

batches of barramundi

– 9 m3 of rotifer culture tanks (900 rot./ml) enough for 3 mill. Eggs/batch

– Reduced Artemia cyst from 40 kg to 20 Kg for 1 million eggs

– Survival rates from hatching to 3cm TL = 38-42%

– Deformity rates < 2,7%

Center for Aquatic Animal Health and Breeding Studies

– 350 g hybrid grouper eggs – 58,000 fingerlings of 6-8 cm

– 200 g of E. malabaricus egg – 62,000 fingerlings of 8-10 cm – Deformity rates were 3.1 and 2.3 % respectively

Cương Lan Co., Ltd in Nha Trang city – 2 batches of barramundi

– Rotifer culture volume reduced from 60 m3 to 10 m3 for 4 million egg batch

– Deformity rates < 1,8%


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MAIN POINTS SUGGESTED TO BE

CONSIDERED IN CURENT HATCHERY PROTOCOL

FOR EFFICIENT HATCHERY PRODUCTION

Algae

Rotifer

Artemia

Formulated feeds

Day old

0 402 251410 18 60 80 APPROPRIATE GREENWATER CONDITION

CLEANED ROTIFER TANK

CORRECT ROT. QUANTIFICATION

ROTIFER FEED QUALITYPROPER ENRICHMENT

SELECT SUFFICIENT CYST

IMPROVE HATCHING EFF.

PROPER ENRICHMENT


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THANK YOU VERY MUCH FOR YOUR

COLLABORATION AND SUPPORT

aquaculture products in vietnam

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