aquaculture biosecure dr...definition of bioflocs by francois brenta ... biosecurity in shrimp...

Download Aquaculture Biosecure Dr...Definition of Bioflocs By Francois Brenta ... BIOSECURITY IN SHRIMP FARMING –Indoor Biofloc Systems Applied to Broodstock Production Heterotrophic Bacteria

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  • byFrancoisBrenta

    AquacultureBiosecureSystems

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    Source:2016SurveyfromtheGlobalAquacultureAlliance

    ByFrancoisBrenta

    Diseases ***Production costs - Feed/Fishmeal

    Seed stock quality & availability Access to disease-free broodstock

    ***International market prices Production costs - Others

    Product quality control Environmental management Feed quality and availability

    Production costs - Fuel Access to Credit

    Banned chemicals / antibiotic use ***International trade barriers

    Market coordination Infrastructure

    Public Relations Management Conflicts with other users

    Not Important Extremely Important Moderately Important

    GOAL'2016'Survey'Issues'&'Challenges'in'Shrimp'Aquaculture''

    All'Countries'

    Asterisk indicates a Top 3 issue in GOAL 2007 Survey

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    BroodstockSupplyChain

    ByFrancoisBrenta

    Sub-adults

    Juveniles

    PLs

    Nauplii

    Broodstock

    Nauplii

    Broodstock

    PLs

    Nauplii

    Sub-adults

    Juveniles

    PLs

    Processing

    ImportedSPFBroodstock

    Broodstock

    Quarantine Breeding Maturation Hatchery Farm

    WildBroodstock

    PondraisedBroodstock

    Pondsurvivors

    HighRisk!!!

    Biosecure

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    ReasonforLackofSupplyofDiseaseFreeBroodstock

    ByFrancoisBrenta

    q Wild broodstock!!!! Extremely high biosecurity risk.

    q Outdoor broodstock production!!! Very High biosecurity risk.

    q Lack of biosecurity in indoor broodstock production facilities!! High biosecurity risk. Inadequate shrimp health monitoring programs. Unable to achieve and maintain

    health status. Inadequate water treatment. Unable to bio-remediate to rationalize water

    management. Inadequate zoning and insufficient access restriction.

    q Insufficient biosecure indoor broodstock production facilities! High business risk.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    WhyBioflocTechnology

    ByFrancoisBrenta

    qCan sustain high carrying capacity.

    qCost-effective bio-remediation tool.

    qReduces very significantly water exchange requirements.

    q Low capital expenditure vs. other bio-remediation systems such as RAS.

    qReduces FCR and wastes in water column.

    qEnhances natural gonadal development in broodstock.

    q Low environmental impact.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    DefinitionofBioflocs

    ByFrancoisBrenta

    qAggregates formed by interaction between particulate organic matter and a largerange of microorganisms, such as bacteria, (phytoplankton), rotifers, ciliatesflagellates protozoa and copepods.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    TheBioflocSystem

    ByFrancoisBrenta

    TAN[NH3 +NH4+ ]

    FecesExcretionFeedwaste

    NO2

    NO3(Non-toxic)

    Feeds

    Corg

    (C:Nratio)

    MicrobialBiomass

    Cinorg+

    Oxygen

    NitrifyingBacteria

    PhysicalSubstrate

    Uptakeofnitrogencompoundsgeneratinginsitumicrobialproteinandmaintainingwaterquality.

    Improvingcost-efficiencybyreducingFCRandproteindemand

    Naturalgonadaldevelopment

    Improvinghealthconditionsbystimulatingimmuneresponse

    Reducesverysignificantlywaterrequirements.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    HeterotrophicBacteria

    ByFrancoisBrenta

    AssimilateAmmoniaintoprotein

    Consumeorganiccarbon

    Veryfastcellduplication

    Formthebiofloc

    Imhoffconeshowingbioflocsettled

    Floclevelwillincreasewiththegrowthofheterotrophicbacteria

    HeterotrophicbacteriaBacillusspp.

    Molasses as carbon source applications, followed bydrop of TAN levels. Nitrogen assimilated asheterotrophic bacteria biomass. Da Silva et al., 2013.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    Chemo-autotrophicBacteria

    ByFrancoisBrenta

    NitrifyingBacteria

    Lateestablishmentinthesystem

    Requiretobeattachedforeffectivenitrification

    Consumeinorganiccarbon(alkalinity)

    Inoculumand/orProbiotics

    AmmoniaOxidizerBacteria(AOB) Nitrosomonas,Nitrosococcus,Nitrosospira Oxidizeammoniaintonitrite(NO2)

    NitriteOxidizerBacteria(NBO) Nitrobacter,Nitrococcus,Nitrospira OxidizeNO2 intonitrate(NO3)nontoxic

    Nitrification process in biofloc system where nitriteis being oxidized into nitrate. Da Silva et al., 2013.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    BioflocSystemDynamics

    ByFrancoisBrenta

    qImmatureSystem

    Heterotrophicpathway100%ofNrecyclingApplicationofmolassesandbicarbonates

    Increaseinbioflocs(surfacearea) Proteinsourceandboostsimmunesystem

    qMatureSystem

    Nitrificationprocessestablished Chemo-autotrophicpathway 65%ofNrecycling

    Heterotrophicpathway 35%ofNrecycling Carbonprovidedbyfeeds(organic)andalkalinity(inorganic)

    ApplicationsofbicarbonatesOccasionalapplicationsofmolasses

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    ImpactofBioflocsonWaterQualityandWaterConsumption

    ByFrancoisBrenta

    BFT

    60%lessNitrogenwastesand70%lessPhosphoruswastesthanconventional

    system.

    Recyclingnitrogenintobacterialprotein

    Establishmentofmicrobialfoodchain(protein-rich)

    TransferofNintoshrimpbiomass

    MaintenanceofNinnon-toxiclevels

    Lessgenerationofwaste

    Waterexchangedecreasesfrom50-100%perdayto5%-10%perweek.

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    ImpactofBioflocsonFeedConsumption

    ByFrancoisBrenta

    30%- 40%ofshrimpsbiomassisobtainedbybioflocconsumptionin

    BFTsystem(Burfordetal.,2004;Cardonaetal.,2015)

    Crude ProteinofBioflocs Reference

    43% McIntoshetal.,2000

    12- 42% Soares etal.,2004

    26- 41.9% Ju etal., 2008

    31% Tacon etal.,2010

    38.8- 40.5% Kuhnetal.,2010

    28- 43% Maic etal.,2012

    ReduceFCR

    Reduceproteindemandinthefeeds

    Consequentlyreducesfeedcosts

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    ImpactofBioflocsontheHealthoftheShrimp

    ByFrancoisBrenta

    Microbialflocsarerichinpolysaccharides,taurineandfatsolublevitamins,allofwhichcancontributetoahealthystatusoftheprawns.Thecontentsofbioflocscanbeinfluencedbyinputsandlightexposure.

    Biofloc has bioactive compounds that contribute for a healthy statusof cultured prawns (Ju et al., 2008b)

    Expressions of certain haemocytes enzymes related to immunesystem is enhanced in biofloc reared L. vannamei (Jang et al., 2011)

    Bioflocs have positive effect in the immune response of L. vannameileading to higher resistance against IMNV challenge (Ekasari et al., 2014)

    Immune system and antioxidants enhanced in L. vannamei juvenilesreared in biofloc (Xu & Pan, 2013)

    Prawns show resistance to Vibrio spp when reared in BFT (Liu et al., 2017)

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    BioflocManagement

    ByFrancoisBrenta

    Fullscaleproductionfrom

    broodstocktobroodstockin300

    DOC.

    Maturation-larval:60DOC.

    Nursery:60DOCfromPLto2g.

    Grow-out:120DOCfrom2gto

    20g.

    Pre-conditioning:60DOCfrom

    20gto35g.

    100m2 ~100m3 forproducing

    1000broodstock.

    Costperbroodstock20-40USD.

    Concreteraceways

    Aerationwithairlifts

    Greenhouse aerationwithporousdiffusers

    Greenhouse aerationwithpaddle-wheels

  • BIOSECURITYINSHRIMPFARMING IndoorBioflocSystemsAppliedtoBroodstockProduction

    BioflocManagement

    ByFrancoisBrenta

    qFeeding

    Feedtrays

    Wastesfromsyphoning

    ABW FeedRates DailyMaximumFeedLoad(g/m3)vs.ABW(g)atspecificShrimpLoads(g/m3)- Rule:Keep