Indonesian Crab Development CenterCBAD TAKALAR - SOUTH SULAWESI

APLICATION OF PROBIOTIC AND MOLASES FOR IMPROVING OF REARING MEDIA ON CRABLET MASS PRODUCTION OF

SWIMMING CRAB (Portunus pelagicus, Linn)

Sugeng RaharjoNana S.S. Udi PutraEddy NurcahyonoMakulau Sultan

Introduction• CBAD Takalar has been producing crablet of

Portunus pelagicus since 2005• Crablet production was unstable• Attacking of pathogen bacteria (Vibrio sp) caused

a production loss • Environmant was become an issue global-food

safety and human health• Preventive treatment: probiotic added for

reduction pathogen bacteria but its has a short life time, so need the substrute for feeding

• Probiotic plays as a biological balance for bacteria pathogen like Vibrio sp.

Objective

• To know the effectiveness of probiotic bacteria and molases toward increasing the survival rate of swimming crab larvae

Materials and methods

• Probiotic : Commercial product ”Develop TM” • Molases: Sugarcan factory in Takalar – South

Sulawesi Indonesia

Materials and Methods• Conical fiber pond : 250 L• Stocking density : 60 ind/mL• Rearing period : 15 days• Diet :

zoeae-1 to zoeae-4 : Branchionus plicatilis (density 10-15 ind/ml) and fatty acid Ω3– HUFA (100 ppm) and multivitamin (100 ppm)zoeae-3 until finish : Artemia salina (4 times per day)

• Treatments : 1. Probiotic : 10 ppm2. Probiotic with molases : 10 ppm of probiotic

and 100 ppm of molases

• added every 3 day after water exchange

Materials and Methods

Data Collecting and Analysis

• Growth stage of larvae• Survival rate• Population of bacteria • Water quality

Results & Discussion

REARING PERIOD (day)

TREATMENTS

ROBIOTIC PROBIOTIC AND MOLASES

1Zoeae-1

Zoeae-1

2 Zoeae-1 Zoeae-1

3 Zoeae-1 Zoeae-1(10%)/ Zoeae-2 (90%)

4Zoeae-2

Zoeae-2

5 Zoeae-2

Zoeae-3

6 Zoeae-3

Zoeae-3

7 Zoeae-3 Zoeae-4

8 Zoeae-4 Zoeae-4

9Zoeae-4

Zoeae-4 (40%)/Megalop-1 (60%)

10Zoeae-4(90%)/ Megalop-1 (10%) Megalop-1

11 Megalop-1 Megalop-2

12 Megalop-2 Megalop-3

13 Megalop-3

Megalop-4

14 Megalop-4

Megalop-5 (80%)/Crablet-1(20%)

15 Megalop-5/Crablet-1 (10%)

Crablet-1

Table 1. Growth stage development of swimming crab larvae for 15 days rearing periode

GRO

WTH

S

TAG

E

1 2 3 4 5 6 7 8 9 10 11 12 13 14 150

102030405060708090

100

Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet

Rearing of the day

Po

pu

lati

on

of

Gro

wth

sta

ge

(%

)

1 2 30

10

20

30

40

50

60

70

80

90

100

Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet

Rearing period

Po

pu

lati

on

of

Gro

wth

sta

ge

(%

)

Figure 1. Growth stage development of swimming crab larvae (A) Treatment of Probiotic and molases and (B) Treatment of probiotic only.

B

APo

pula

tion

of g

row

th st

age

(%)

20%

90%

20% 10%

60%

Addition of molases into water rearing media of swimming crab was accelerated growth stage. Its mean that larvaes have a high growth performance than using probiotic only.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 150.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

Probiotic

Probiotic and Mo-lases

Rearing period

Le

ng

ht

of

ca

rap

ac

e (

mm

)

1 2 3 4 5 6 7 8 9 10 11 12 13 14 150.0000

0.0002

0.0004

0.0006

0.0008

0.0010

0.0012

0.0014

Probiotic

Probiotic and Mo-lases

We

igh

t (g

)

Figure 2. Growth of swimming carb larvae in rearing period (A) body weight and (B) lenght of carapace

A

B

Body weight and Lenght of carapace of probiotic and molases treatment were more lenght and weight than treatment probiotic only but not significantly different (P>0,05).Have a tending that probiotic and molases treatment was higher performance growth

•Addition of molases into water rearing media of swimming crab tends to reduce the growth stage, as larvae growth was optimal.

•In the final rearing period showed lenght of carapace and weight body of treatment probiotic and molases were more lenght and weight than treatment of probiotic only but not significantly different (P>0,05).

But unsignificant different between both treatment might be caused by molases concentration and C-N ratio of feed were not optimal yet

Figure 3. Survival rate of each stage on rearing period of swimming crab larvae

Z1 Z2 Z3 Z4 M C0

20

40

60

80

100

ProbioticProbiotic and Molases

Growth stage

Su

rviv

al r

ate

(%

)

SURV

IVAL

RAT

E

Survival rate of probiotic and molases treatment were higher survival rate than treatment probiotic only but not significantly different (P>0,05) for Z2 and Crablet stage.Thereby Probiotic and molases played an important role in increasing SR.

Treatments Parameters Growth Stage

Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalop

Probiotic Bacteria TotalVibrio sp

2,4 X 103

2,0 X 102

2,0 X 104

2,0 X 103

3,2 X 104

1,2 X 103

1,1 X 103

2,1 X 102

6,0 X 104

4,2 X 103

Probiotic and Molases

Bacteria TotalVibrio sp

2,0 X 104

3,2 X 103

3,4 X 104

3 X 102

4,6 X 104

3,2 X 102

4,2 X 106

2,2 X 102

4,2 X 106

2 X 102

Table 2. Population density of bacteria total and Vibrio sp. on each growth stage (cfu/ml).

POPU

LATI

ON

OF

BACT

ERIA

Treatment of probiotic and molases was higher population density of bacteria total than treatment of probiotic only. Its indicated that molases plays an important role in providing nutrition for probiotic.

In the both treatments showed that the presence of population of probiotic bacteria will be able to inhibit the population of bactera pathogen. Aplication probiotic in the water media will have benefit more if probiotic could growth and developed well, furthermore nutrition should be provided. So in this research addition of molases as substrate and nutrition also important.

Parameters Treatment

Probiotic Probiotic dan Molases

Dissolved Oxygen (mg/l)pHTemperature (0C)Salinity (ppt)Ammonia (mg/l)Nitrit (mg/l)

4,8 – 5,67,8 – 8,627 – 3133 – 35

Not detectedNot detected

4,6 – 6,28,1 – 8,427 – 3133 -35

Not detectedNot detected

Table 3. Water quality performance in both treatments probiotic and probiotic with molases of rearing of swimming crab larvae (Portunus pelagicus,linn)

WAT

ER Q

UAL

ITY

Conclusion & RecommendationApplication of the probiotic bacteria with molases in water media of swimming

crab larvae (Portunus pelagicus,linn) tends to increase the survival rate, body weight, lenght of carapace, and able to accelerate the growth of zoeae stage, in addition also controlled the population growth of Vibrio sp (102 cfu/ml). Furthermore using probiotic with molases was effective in improving of rearing water media on mass producing crablet of swimming crab.

For the further it should be conducted the detail study in using of concentration molases, probiotic density and other combination substrate for getting the high survival rate and growth such as C-N ratio.

Further Information

CENTER FOR BRACKISHWATER AQUACULTURE DEVELOPMENT TAKALAR

ADDRESS :BONTOLOE VILLAGE SOUTH GALESONG –TAKALAR 92245

SOUTH SULAWESI – INDONESIATEL : 62-411-320730FAX: 62-418-2327666

SUCC

ESS A

ND THANK