aph161 - lecture 11: the cytoskeleton is always under construction

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APh161 - Lecture 11: The Cytoskeleton is APh161 - Lecture 11: The Cytoskeleton is Always Under Construction Always Under Construction Rob Phillips California Institute of Technology Mullins et al.

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Rob Phillips California Institute of Technology. APh161 - Lecture 11: The Cytoskeleton is Always Under Construction. Mullins et al. Polymerization Processes 1: Tobacco Mosaic Virus. Caspar. A reminder on the cytoskeleton. Cytoskeletal Action During Cell Division. - PowerPoint PPT Presentation

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Page 1: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

APh161 - Lecture 11: The Cytoskeleton is APh161 - Lecture 11: The Cytoskeleton is Always Under ConstructionAlways Under Construction

Rob PhillipsCalifornia Institute of Technology

Mullins et al.

Page 2: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Polymerization Processes 1: Tobacco Polymerization Processes 1: Tobacco

Mosaic VirusMosaic Virus

Caspar

Page 3: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

A reminder on the cytoskeletonA reminder on the cytoskeleton

Page 4: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Cytoskeletal Action During Cell DivisionCytoskeletal Action During Cell Division

Page 5: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Cytoskeleton Mediated MitosisCytoskeleton Mediated Mitosis

Show the movies in the Lecture 11 directory – see http://www.bio.unc.edu/faculty/salmon/lab/mitosis/mitosismovies.html

Page 6: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Cell Division in BacteriaCell Division in Bacteria

Petra Levin

FtsZ rings during division

Page 7: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Actin Polymerization Rate in vitroActin Polymerization Rate in vitro

Page 8: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Actin and its PartnersActin and its Partners

Page 9: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Life at the Leading EdgeLife at the Leading Edge

Page 10: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Microtubule Dynamics: Part of the Microtubule Dynamics: Part of the

Polymerization PuzzlePolymerization Puzzle

Page 11: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Microtubule Dynamics In VitroMicrotubule Dynamics In Vitro

Show Borisy movies,http://www.borisylab.nwu.edu/pages/supplemental/treadmill.html

Flyvbjerg et al., 1996

Catastrophe rate vs. growth rate

Page 12: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

The Story of ParMThe Story of ParM

(Dyche Mullins et al.)

Page 13: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

ParM Length DistributionParM Length Distribution

Mullins et al., Science 2004

Page 14: APh161 - Lecture 11: The Cytoskeleton is Always Under Construction

Polymerization of Pyrene Labeled Polymerization of Pyrene Labeled

ActinActin

Lim et al., Science 2004