antimalarial pp presentation
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Antimalarial Artemisinin
Michelle AtkinsJavier Ramirez
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What is Malaria?
A disease of the blood caused by the Plasmodium parasite.
100+ species of malaria parasite exists.
The most common is Plasmodium falciparum.
214M cases worldwide in 2015.
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Anti-Malarial Artemisinin Works faster against Uncomplicated
Malaria. Artemisinin is extracted from the
plant Artemisia Annua: Used in Chinese Traditional
Medicine.
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The Problem
Artemisinin Molecule
A. Annua plant
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Objectives
Find a stable source of affordable artemisinin.
Produce high yield biological strains of arteminisinic acid.
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Hypothesis
“The discovery of a plant dehydrogenase and a second cytochrome will provide an efficient
biosynthetic route to artemisinic acid”.
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The Amyris Approach
2. Automated DNA Assembly
1. Design Biosynthetic Pathway
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Automated DNA Assembly (Cont.)
Next Generation Sequencing
DNA Regions are assembled in vivo in yeast
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Hi-RYSE (Hyper-Integration for Rapid Yeast Strain Engineering)
Several new DNA designs
Single step strain conversion
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Pathway to Artemisinic Acid
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Synthesis of Artemisinin
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Purification Optimization
Feedback Controlled Pulse-Feed Extractive Fermentation Process Increases the Yield from 8 g/L to 28 g/l.
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Conclusion Plant Extraction
Mass Production Worldwide Supply
Yeast