announcements raise your hand if using pubcrawler?
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Announcements
•Raise your hand if using Pubcrawler?
Announcements
•No recitations tomorrow.
•Write researcher for mutant DNA yet?
• If one primer anneals at 50oC and the other at 60oC what’s a good temp for your PCR annealing?
• Raise your hand if using Pubcrawler?
If primer #1 is calculated to anneal at 50oC and primer #2 at 60oC what’s a good temp to try for your annealing temperature in the first PCR experiment you do?a. 45oCb. 50oCc. 55oCd. 60oCe. 65oC
Facilitated Diffusion
Osmosis (diffusion of water)
Water follows solute (salt)HEY!
If the membrane is permeable to water and monosaccharides…1. Which solutes will diffuse into the cell?2. In which direction will water move?3. Is the cell’s solution iso, hypo, or hypertonic to the beaker’s?4. Will the cell die?
An artificial cell with an aqueous solution enclosed in a selectivelypermeable membrane has just been immersed in this beaker.
If the membrane is permeable to water and monosaccharides…1. In which direction will water move?A. Into the cell causing it to swell upB. Out of the cell causing it to shrinkC. Equally in both directions
An artificial cell with an aqueous solution enclosed in a selectivelypermeable membrane has just been immersed in this beaker.
If the membrane is permeable to water and monosaccharides…2. Is the cell’s solution iso, hypo, or hypertonic to the beaker’s?A. The cell’s solution is isotonic to the beaker’sB. The cell’s solution is hypotonic to the beaker’sC. The cell’s solution is hypertonic to the beakers
An artificial cell with an aqueous solution enclosed in a selectivelypermeable membrane has just been immersed in this beaker.
ATP ADP + P
Active Transport
binding
hydrolysis
release
Based on the model of sucrose uptake shown, which of the following treatments would increase the rate of sucrose transport into the cell.a. Decreasing extracellular sucrose concentration.b. Decreasing extracellular pHc. Decreasing cytoplasmic pHd. Adding an inhibitor that blocks the creation of ATP.e. Adding a substance that makes the membrane more permeable to H+
cytoplasm outside cell
Based on the model of sucrose uptake shown, which of the following treatments would increase the rate of sucrose transport into the cell.a. Decreasing extracellular sucrose concentration.b. Decreasing extracellular pHc. Decreasing cytoplasmic pHd. Adding an inhibitor that blocks the creation of ATP.e. Adding a substance that makes the membrane more permeable to H+
cytoplasm outside cell